CD42a antibody | FMC-25

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Mouse anti Human CD42a:FITC

Mouse anti Human CD42a

Mouse anti Human CD42a:RPE

Product Type
Monoclonal Antibody
Clone
FMC-25
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA594F F 0.1 mg
MCA594GA C F 0.1 mg
MCA594 C F 0.25 mg
MCA594PE F 100 Tests
Mouse anti Human CD42a antibody, clone FMC-25 recognizes human CD42a, also known as Platelet glycoprotein IX, Glycoprotein 9 or GP-IX. CD42a is a 177 amino acid, ~20kDa type I single pass transmembrane glycoprotein containing a single leucine-rich repeat containing N-terminal domain and a single leucine-rich repeat containing C-terminal domain.

CD42a is expressed by platelets and megakaryocytes and forms a covalent complex with CD42c (GP-1b-beta), CD42b (GP-1b-alpha) and CD42d (platelet glycoprotein V) to create the platelet surface receptor for von Willebrand factor. Incubation of the intact von Willebrand receptor complex with clone FMC-25 does not appear to inhibit binding of von Willebrand factor to the receptor (Yan et al. 2011). Defects in the GP1BB gene encoding human CD42a can lead to the inherited bleeding disorder Bernard-Soulier syndrome (Diz-Küçükkaya 2013), characterized by prolonged bleeding times, thrombocytopenia and the appearance of giant platelets in the circulation (Johns et al. 2014).

Mouse anti human CD42a antibody, clone FMC-25 has been successfully used as a capture reagent for platelet-autoantibody complexes in the sera of patients presenting thrombocytopenia associated with antiphospholipid syndrome (Godeau et al. 1997).

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Carrier Free
Yes
Immunogen
Peripheral blood mononuclear cells.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
Pack Size: 0.1 mg
IgG concentration 1.0mg/ml
Pack Size: 0.25 mg
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P14770 Related reagents
Entrez Gene
GP9 Related reagents
GO Terms
GO:0005515 protein binding
GO:0007155 cell adhesion
GO:0005887 integral to plasma membrane
GO:0007597 blood coagulation, intrinsic pathway
GO:0030168 platelet activation
Regulatory
For research purposes only

Applications of CD42a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/50 1/200
Immunohistology - Frozen
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Histology Positive Control Tissue
Bone marrow
Histology Positive Control Tissue
Bone marrow
Histology Positive Control Tissue
Bone marrow

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

ELISA

Product Specific References

References for CD42a antibody

  1. Zola, H. et al. (1984) Monoclonal antibodies against antigens of the human platelet surface: preparation and properties.
    Pathology. 16 (1): 73-8.
  2. Berndt, M.C. et al. (1985) Molecular characterization of quinine/quinidine drug-dependent antibody platelet interaction using monoclonal antibodies.
    Blood. 66 (6): 1292-301.
  3. Berndt, M.C. et al. (1985) Purification and preliminary characterization of the glycoprotein Ib complex in the human platelet membrane.
    Eur J Biochem. 151 (3): 637-49.
  4. Berndt, M.C. et al. (1983) Additional glycoprotein defects in Bernard-Soulier's syndrome: confirmation of genetic basis by parental analysis.
    Blood. 62 (4): 800-7.
  5. San Miguel, J.F. et al. (1985) Characterization of blast cells in chronic granulocytic leukaemia in transformation, acute myelofibrosis and undifferentiated leukaemia. II. Studies with monoclonal antibodies and terminal transferase.
    Br J Haematol. 59 (2): 297-309.
  6. San Miguel, J.F. et al. (1986) Surface marker analysis in acute myeloid leukaemia and correlation with FAB classification.
    Br J Haematol. 64 (3): 547-60.
  7. Smith GA et al. (2007) Severe fetomaternal alloimmune thrombocytopenia due to anti-human platelet antigen (HPA)-1a in a mother with a rare and silenced ITGB3*0101 (GPIIIa) allele.
    Vox Sang. 93 (4): 325-30.
  8. Berndt, M.C. et al. (1988) Ristocetin-dependent reconstitution of binding of von Willebrand factor to purified human platelet membrane glycoprotein Ib-IX complex.
    Biochemistry. 27 (2): 633-40.
  9. Yan, R. et al. (2011) Reconstitution of the platelet glycoprotein Ib-IX complex in phospholipid bilayer Nanodiscs.
    Biochemistry. 50: 10598-606.
  10. Sailer, T. et al. (2006) The course of severe autoimmune thrombocytopenia in patients not undergoing splenectomy.
    Haematologica. 91: 1041-5.
  11. Tomicic, M. et al. (2006) Frequency of HPA-15a and HPA-15b (Gov a/b) human platelet alloantigens in the Croatian population.
    Arch Med Res. 37: 172-4.
  12. Starcevic, M. et al. (2010) Neonatal alloimmune thrombocytopenia caused by anti-HLA-A24 alloantibodies.
    Acta Paediatr. 99: 630-2.
  13. Schallmoser, K. et al. (2006) Specificities of platelet autoantibodies and platelet activation in lupus anticoagulant patients: a relation to their history of thromboembolic disease.
    Lupus. 15: 507-14.
  14. Meyer, O. et al. (2003) Diclofenac-induced antibodies against RBCs and platelets: two case reports and a concise review.
    Transfusion. 43: 345-9.
  15. Lubenow, N. et al (2000) Very low platelet counts in post-transfusion purpura falsely diagnosed as heparin-induced thrombocytopenia. Report of four cases and review of literature.
    Thromb Res. 100: 115-25.
  16. Ghevaert, C. et al. (2008) A nonsynonymous SNP in the ITGB3 gene disrupts the conserved membrane-proximal cytoplasmic salt bridge in the alphaIIbbeta3 integrin and cosegregates dominantly with abnormal proplatelet formation and macrothrombocytopenia.
    Blood. 111: 3407-14.
  17. Bub, C.B. et al. (2016) The use of a potential novel tool in virtual crossmatching for platelet transfusion in platelet refractoriness.
    Vox Sang. 110 (1): 70-8.
  18. Michel, M. et al. (2002) Platelet autoantibodies and lupus-associated thrombocytopenia.
    Br J Haematol. 119 (2): 354-8.
  19. Schallmoser, K. et al. (2006) Delayed detectability of anti-HPA-3a by the MAIPA assay in a severe neonatal alloimmune thrombocytopenia, but successful transfusion of incompatible donor platelets: a case report.
    Vox Sang. 91 (2): 181-3.
  20. Wihadmadyatami, H. et al. (2015) Alloantibody against new platelet alloantigen (Lap(a)) on glycoprotein IIb is responsible for a case of fetal and neonatal alloimmune thrombocytopenia.
    Transfusion. 55 (12): 2920-9.