CD326 antibody | MOC-31
Mouse anti Human CD326 antibody, clone MOC-31 (MCA2162XZ) used for the detection of CD326 positive cells by immunohistochemistry on formalin fixed, paraffin embedded prostate tissue sections.
Image caption:
Immunohistochemical analysis of Ep-ICD and EpEx in prostate tissues. Panel shows (a) Nuclear/Cytoplasmic Ep-ICD immunostaining in (I) Normal, (II) BPH, and Prostate Adenocarcinoma: (III) Gleason Score <7, (IV) Gleason Score = 7; and (V) Gleason Score >7. Panel (b) Membrane EpEx immunostaining in (I) Normal (II) BPH, and Prostate Adenocarcinoma (III) Gleason Score <7, (IV) Gleason Score = 7; and (V) Gleason Score >7. Arrows show nuclear (N) Ep-ICD staining and Membrane (M) EpEx staining (Original Magnification x 400).
From: Assi J, Srivastava G, Matta A, MacMillan C, Ralhan R, et al. (2015)
Nuclear Ep-ICD Expression Is a Predictor of Poor Prognosis in “Low Risk” Prostate Adenocarcinomas.
PLoS ONE 10(2): e0107586.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD326 antibody, clone MOC-31 (MCA2162XZ) used for the detection of CD326 positive cells in tumor tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis of EpEx, Ep-ICD and β catenin in thyroid cancer. The ATC did not show detectable membranous EpEx staining (IA); all the other subtypes of TC analyzed and normal thyroid tissues showed varying levels of plasma membranous EpEx staining (IB-IF). Nuclear Ep-ICD staining was observed in undifferentiated and poorly differentiated TC (IIA-IIC, and IIF), but not in well differentiated TC and the adjacent normal thyroid tissue (IID, IIE). Correlated with nuclear Ep-ICD staining, nuclear or cytoplasmic β catenin staining was observed in aggressive TC (IIIA-IIIC, and IIIF), while membranous staining was observed in the less aggressive TC and the adjacent normal thyroid tissues (IIID, IIIE). Panels A, B and F- I and II, C and F III- original magnification × 40; panels A III, B III, C, D, E - I and II, D and E III- original magnification × 20. The abbreviations M, C, and N denote membranous, cytoplasmic and nuclear localization of the proteins.
From: Ralhan R, Cao J, Lim T, Macmillan C, Freeman JL, Walfish PG.
EpCAM nuclear localization identifies aggressive thyroid cancer and is a marker for poor prognosis.
BMC Cancer. 2010 Jun 25;10:331.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD326 antibody, clone MOC-31 (MCA2162XZ) used for the detection of CD326 positive cells in tumor tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Differential immunohistochemical expression of EpEx, Ep-ICD and β-catenin proteins in different regions of an ATC tumor with varying degrees of aggressive tumor pathology. No membranous EpEx staining was observed in the ATC tissue section (IA), faint membranous EpEx staining in tissue section showing squamous cell carcinoma (IB), membranous EpEx staining in both poorly differentiated TC section and the adjacent normal thyroid tissue section (IC, ID). Nuclear and cytoplasmic Ep-ICD staining in undifferentiated and poorly differentiated TC tissue sections (IIA-IIC), membranous and cytoplasmic staining in the adjacent thyroid normal tissue (IID). Nuclear and cytoplasmic β catenin staining in ATC section (IIIA), membranous β catenin staining in different regions of this tumor with varying degrees of aggressive tumor pathology (IIIB-IIID). A-D, original magnification × 10.
From: Ralhan R, Cao J, Lim T, Macmillan C, Freeman JL, Walfish PG.
EpCAM nuclear localization identifies aggressive thyroid cancer and is a marker for poor prognosis.
BMC Cancer. 2010 Jun 25;10:331.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD326 antibody, clone MOC-31 (MCA2162XZ) used for the detection of CD326 positive cells in epithelial tumor tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis of EpEx expression in epithelial cancers. The photomicrographs depict MOC31 stained membrane EpEx in epithelial cancers. The panel I shows low level of membrane EpEx expression in normal breast (A) and prostate (B, Ia), BPH (B, Ib), and normal esophagus (C) and head and neck (D) tissues. The corresponding cancer tissues depicting increased level of EpEx in the membrane are shown in panel II (A–D). In contrast many of the cancer tissues of each cancer type showed absence of membrane EpEx (panel III, A–D). Original magnification ×400.
From: Ralhan R, He HC-H, So AK-C, Tripathi SC, Kumar M, et al. (2010)
Nuclear and Cytoplasmic Accumulation of Ep-ICD Is Frequently Detected in Human Epithelial Cancers.
PLoS ONE 5(11): e14130.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD326 antibody, clone MOC-31 (MCA2162XZ) used for the detection of CD326 positive cells in epithelial tumor tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
EpEx immunohistochemical analysis in epithelial cancers control tissues. The negative control photomicrographs are shown. HNSCC (A), prostate cancer (B), colon cancer (C), breast cancer (D), ESCC (E) and normal esophagus (F); panels G and H are positive controls for EpEx staining. Original magnification ×400.
From: Ralhan R, He HC-H, So AK-C, Tripathi SC, Kumar M, et al. (2010)
Nuclear and Cytoplasmic Accumulation of Ep-ICD Is Frequently Detected in Human Epithelial Cancers.
PLoS ONE 5(11): e14130.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD326 antibody, clone MOC-31 (MCA2162XZ) used for the detection of CD326 positive cells in epithelial tumor tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Fluorescence immunostaining in CX-1 cells with anti-EpEx (MOC31) and anti-Ep-ICD antibodies. Secondary antibodies are FITC-anti-mouse (green) and TRITC-anti-rabbit (red). A) EpEx; B) Ep-ICD; C) DAPI; D) EpEx and DAPI (A&C merged); E) Ep-ICD and DAPI (B & C merged); F) EpEx and Ep-ICD (A & B merged); G) EpEx, Ep-ICD, and DAPI (A, B, C merged); I) Measurement of the density of three colors across the line in the cells in H.
From: Ralhan R, He HC-H, So AK-C, Tripathi SC, Kumar M, et al. (2010)
Nuclear and Cytoplasmic Accumulation of Ep-ICD Is Frequently Detected in Human Epithelial Cancers.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD326 antibody, clone MOC-31 (MCA2162XZ) used for the detection of CD326 staining by immunohistochemistry of formalin fixed, paraffin embedded tumor tissue sections.
Image caption:
Immunohistochemical analysis of EpEx and Ep-ICD expression in papillary thyroid carcinomas and benign tissues. The representative photomicrographs show immunostaining of EpEx and Ep-ICD in paraffin-embedded thyroid benign nodule goiters, non-aggressive PTC and aggressive PTC tissues. Strong membranous EpEx immunostaining was observed in benign cases (A) and non-aggressive PTC tissues (C); reduced staining of membrane EpEx was observed in aggressive PTC cases (E, G). The benign thyroid nodules and non-aggressive PTC (D) showed predominant cytoplasm localization of Ep-ICD and no detectable nuclear Ep-ICD staining (B, D), while the aggressive PTC cases showed strong nuclear and cytoplasmic Ep-ICD accumulation (F, H). M, membrane staining; C, cytoplasmic staining; N, nuclear staining; Loss of M, loss of membrane expression. Original magnification × 400.
From: He HC-H, Kashat L, Kak I, Kunavisarut T, Gundelach R, et al. (2012)
An Ep-ICD Based Index Is a Marker of Aggressiveness and Poor Prognosis in Thyroid Carcinoma.
PLoS ONE 7(9): e42893.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD326 antibody, clone MOC-31 (MCA2162XZ) used for the detection of CD326 staining by immunofluorescence on formalin fixed, paraffin embedded tumor tissue sections.
Image caption:
Fluorescence immunostaining with anti-EpEx and anti-Ep-ICD antibodies in aggressive and non-aggressive papillary thyroid carcinomas. Secondary antibodies are FITC-anti-mouse (green) and TRITC-anti-rabbit (red). A-F images from a non-aggressive PTC; G-L Images from an aggressive PTC. A,G) EpEx; B,H) DAPI; C, I) Ep-ICD; D) EpEx and DAPI (A & C merged); E) Ep-ICD and DAPI (B & C merged); F) EpEx, Ep-ICD, and DAPI (A, B, C merged). J) EpEx and DAPI (G & I merged); K) Ep-ICD and DAPI (H & I merged); L) EpEx, Ep-ICD, and DAPI (G, H, I merged). M, Membranous staining; C, Cytoplasm staining; N, Nuclear staining. Original magnification × 400.
From: He HC-H, Kashat L, Kak I, Kunavisarut T, Gundelach R, et al. (2012)
An Ep-ICD Based Index Is a Marker of Aggressiveness and Poor Prognosis in Thyroid Carcinoma.
PLoS ONE 7(9): e42893.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD326 antibody, clone MOC-31 (MCA2162XZ) used for the detection of CD326 positive cells by immunohistochemistry on formalin fixed, paraffin embedded breast cancer tissue sections.
Image caption:
Immunohistochemical analysis of EpEx expression in breast cancer. Expression of EpEx in (I) normal breast tissues; (II) DCIS; (III) IDC; (IV) ILC; (V) IMC; (VI) negative control breast cancer tissue incubated with isotype specific IgG showing no detectable immunostaining for EpEx. Membranous EpEx expression was more frequently observed in breast carcinomas compared to normal tissues, except ILC (original magnification × 400). The arrows labeled M depict membrane staining.
From: Srivastava G, Assi J, Kashat L, Matta A, Chang M, Walfish PG, Ralhan R.
Nuclear Ep-ICD accumulation predicts aggressive clinical course in early stage breast cancer patients.
BMC Cancer. 2014 Sep 29;14:726.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD326 antibody, clone MOC-31 (MCA2162XZ) used for the detection of CD326 staining by immunohistochemistry of formalin fixed, paraffin embedded tumor tissue sections.
Image caption:
Immunohistochemical analysis of EpEx and Ep-ICD expression in papillary thyroid microcarcinoma. The representative photomicrographs show immunostaining of EpEx and Ep-ICD in PTMC. Strong membranous EpEx immunostaining was observed in the non-metastatic group (A), whereas decreased staining of membranous EpEx was observed in aggressive PTMC cases (B). The non-metastatic PTMC show predominant cytoplasmic localization of Ep-ICD and no detectable nuclear Ep-ICD staining (C), while the aggressive cases show nuclear and strong cytoplasmic Ep-ICD accumulation (D). (E) depicts the negative control. Original magnification x 400.
From: Kunavisarut T, Kak I, Macmillan C, Ralhan R, Walfish PG.
Immunohistochemical analysis based Ep-ICD subcellular localization index (ESLI) is a novel marker for metastatic papillary thyroid microcarcinoma.
BMC Cancer. 2012 Nov 15;12:523.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD326 antibody, clone MOC-31 (MCA2162) used for the detection of CD326 positive cells in epithelial tumor tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis of EpEx expression in epithelial cancers. Membrane EpEx expression was observed in all the epithelial cancers. Panel I shows intense membrane EpEx in colon cancer (A), liver (B), bladder (C), lung (D), ovarian (E) and pancreatic (F) cancer. The panels II A–F show reduced or absence of membrane EpEx in a subset of each of these epithelial cancers. Original magnification ×400.
From: Ralhan R, He HC-H, So AK-C, Tripathi SC, Kumar M, et al. (2010)
Nuclear and Cytoplasmic Accumulation of Ep-ICD Is Frequently Detected in Human Epithelial Cancers.
PLoS ONE 5(11): e14130.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD326 antibody, clone MOC-31 (MCA2162) used for the detection of CD326 positive cells in epithelial tumor tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis of EpEx expression in epithelial cancers. Membrane EpEx expression was observed in all the epithelial cancers. Panel I shows intense membrane EpEx in colon cancer (A), liver (B), bladder (C), lung (D), ovarian (E) and pancreatic (F) cancer. The panels II A–F show reduced or absence of membrane EpEx in a subset of each of these epithelial cancers. Original magnification ×400.
From: Ralhan R, He HC-H, So AK-C, Tripathi SC, Kumar M, et al. (2010)
Nuclear and Cytoplasmic Accumulation of Ep-ICD Is Frequently Detected in Human Epithelial Cancers.
PLoS ONE 5(11): e14130.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
P IF ResetMouse anti Human CD326:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- MOC-31
- Isotype
- IgG1
- Specificity
- CD326
Antibody Quality and Performance Guarantee
| Mouse anti Human CD326 antibody, clone MOC31 recognizes human CD326, also known as Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial glycoprotein 314, KS 1/4 antigen, Major gastrointestinal tumor-associated protein GA733-2 and Tumor-associated calcium signal transducer 1. Human CD326 is a 314 amino acid ~40 kDa type 1 single pass transmembrane glycoprotein containing a single thyroglobulin domain, CD326 is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. CD326 expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium (Spizzo et al. 2002). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None Present
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20°C only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
10ug/ml | |
| Immunohistology - Frozen | |
10ug/ml | |
| Immunohistology - Paraffin 1 | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
- Histology Positive Control Tissue
- Human lung carcinoma
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD326 antibody
-
Souhami, R.L. et al. (1987) Antigens of small-cell lung cancer. First International Workshop.
Lancet. 2 (8554): 325-6. -
Ralhan R et al. (2010) Nuclear and cytoplasmic accumulation of Ep-ICD is frequently detected in human epithelial cancers.
PLoS One. 5 (11): e14130. -
Dankers, P.Y. et al. (2010) The use of fibrous, supramolecular membranes and human tubular cells for renal epithelial tissue engineering: towards a suitable membrane for a bioartificial kidney.
Macromol Biosci. 10: 1345-54. -
Ralhan, R. et al. (2010) EpCAM nuclear localization identifies aggressive thyroid cancer and is a marker for poor prognosis.
BMC Cancer. 10: 331. -
Kawashima, R. et al. (2011) EpCAM- and EGFR-targeted selective gene therapy for biliary cancers using Z33-fiber-modified adenovirus.
Int J Cancer. 129: 1244-53. -
He HC et al. (2012) An Ep-ICD based index is a marker of aggressiveness and poor prognosis in thyroid carcinoma.
PLoS One. 7 (9): e42893. -
Kunavisarut T et al. (2012) Immunohistochemical analysis based Ep-ICD subcellular localization index (ESLI) is a novel marker for metastatic papillary thyroid microcarcinoma.
BMC Cancer. 12: 523. -
Srivastava G et al. (2014) Nuclear Ep-ICD accumulation predicts aggressive clinical course in early stage breast cancer patients.
BMC Cancer. 14: 726.
View The Latest Product References
-
Assi, J. et al. (2015) Nuclear Ep-ICD expression is a predictor of poor prognosis in "low risk" prostate adenocarcinomas.
PLoS One. 10 (2): e0107586. -
Somasundaram, R.T. et al. (2016) Subcellular differential expression of Ep-ICD in oral dysplasia and cancer is associated with disease progression and prognosis.
BMC Cancer. 16: 486.
Further Reading
-
Winter, M.J. et al. (2003) The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology.
Am J Pathol. 163 (6): 2139-48.
- Synonyms
- Ep-CAM
- RRID
- AB_324459
- UniProt
- P16422
- Entrez Gene
- EPCAM
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0005923 tight junction
- GO:0008284 positive regulation of cell proliferation
- GO:0016323 basolateral plasma membrane
- GO:0016324 apical plasma membrane
- GO:0016328 lateral plasma membrane
MCA2162XZ
155403
159567
167105
167108
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