CD326 antibody | MOC-31

100% Secure


Mouse anti Human CD326:Preservative Free

Product Type
Monoclonal Antibody
Clone
MOC-31
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2162XZ C F P* 1 mg
Mouse anti Human CD326 antibody, clone MOC31 recognizes human CD326, also known as Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial glycoprotein 314, KS 1/4 antigen, Major gastrointestinal tumor-associated protein GA733-2 and Tumor-associated calcium signal transducer 1. Human CD326 is a 314 amino acid ~40 kDa type 1 single pass transmembrane glycoprotein containing a single thyroglobulin domain,

CD326 is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells.

CD326 expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium (Spizzo et al. 2002).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None Present
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P16422 Related reagents
Entrez Gene
EPCAM Related reagents
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0005923 tight junction
GO:0008284 positive regulation of cell proliferation
GO:0016323 basolateral plasma membrane
GO:0016324 apical plasma membrane
GO:0016328 lateral plasma membrane
Regulatory
For research purposes only

Applications of CD326 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 10ug/ml
Immunohistology - Frozen 10ug/ml
Immunohistology - Paraffin 1
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Lung carcinoma

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Product Specific References

References for CD326 antibody

  1. Souhami, R.L. et al. (1987) Antigens of small-cell lung cancer. First International Workshop.
    Lancet. 2 (8554): 325-6.
  2. Ralhan R et al. (2010) Nuclear and cytoplasmic accumulation of Ep-ICD is frequently detected in human epithelial cancers.
    PLoS One. 5 (11): e14130.
  3. Ralhan, R. et al. (2010) EpCAM nuclear localization identifies aggressive thyroid cancer and is a marker for poor prognosis.
    BMC Cancer. 10: 331.
  4. Dankers, P.Y. et al. (2010) The use of fibrous, supramolecular membranes and human tubular cells for renal epithelial tissue engineering: towards a suitable membrane for a bioartificial kidney.
    Macromol Biosci. 10: 1345-54.
  5. Kawashima, R. et al. (2011) EpCAM- and EGFR-targeted selective gene therapy for biliary cancers using Z33-fiber-modified adenovirus.
    Int J Cancer. 129: 1244-53.
  6. Assi J et al. (2015) Nuclear Ep-ICD Expression Is a Predictor of Poor Prognosis in "Low Risk" Prostate Adenocarcinomas.
    PLoS One. 10 (2): e0107586.
  7. Kunavisarut T et al. (2012) Immunohistochemical analysis based Ep-ICD subcellular localization index (ESLI) is a novel marker for metastatic papillary thyroid microcarcinoma.
    BMC Cancer. 12: 523.
  8. He HC et al. (2012) An Ep-ICD based index is a marker of aggressiveness and poor prognosis in thyroid carcinoma.
    PLoS One. 7 (9): e42893.
  9. Srivastava G et al. (2014) Nuclear Ep-ICD accumulation predicts aggressive clinical course in early stage breast cancer patients.
    BMC Cancer. 14: 726.

Further Reading

  1. Winter, M.J. et al. (2003) The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology.
    Am J Pathol. 163 (6): 2139-48.