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CD326 antibody | MOC-31

Mouse anti Human CD326:Preservative Free

Product Type
Monoclonal Antibody
Clone
MOC-31
Isotype
IgG1
Specificity
CD326

Product Code Applications Pack Size List Price Your Price Qty
MCA2162XZ
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P * 1 mg loader
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loader

Mouse anti Human CD326 antibody, clone MOC31 recognizes human CD326, also known as Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial glycoprotein 314, KS 1/4 antigen, Major gastrointestinal tumor-associated protein GA733-2 and Tumor-associated calcium signal transducer 1. Human CD326 is a 314 amino acid ~40 kDa type 1 single pass transmembrane glycoprotein containing a single thyroglobulin domain,

CD326 is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells.

CD326 expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium (Spizzo et al. 2002).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Antibody purified from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None Present
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20°C only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 10ug/ml
Immunohistology - Frozen 10ug/ml
Immunohistology - Paraffin 1
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl
Histology Positive Control Tissue
Human lung carcinoma

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References for CD326 antibody

  1. Souhami, R.L. et al. (1987) Antigens of small-cell lung cancer. First International Workshop.
    Lancet. 2 (8554): 325-6.
  2. Ralhan R et al. (2010) Nuclear and cytoplasmic accumulation of Ep-ICD is frequently detected in human epithelial cancers.
    PLoS One. 5 (11): e14130.
  3. Dankers, P.Y. et al. (2010) The use of fibrous, supramolecular membranes and human tubular cells for renal epithelial tissue engineering: towards a suitable membrane for a bioartificial kidney.
    Macromol Biosci. 10: 1345-54.
  4. Ralhan, R. et al. (2010) EpCAM nuclear localization identifies aggressive thyroid cancer and is a marker for poor prognosis.
    BMC Cancer. 10: 331.
  5. Kawashima, R. et al. (2011) EpCAM- and EGFR-targeted selective gene therapy for biliary cancers using Z33-fiber-modified adenovirus.
    Int J Cancer. 129: 1244-53.
  6. He HC et al. (2012) An Ep-ICD based index is a marker of aggressiveness and poor prognosis in thyroid carcinoma.
    PLoS One. 7 (9): e42893.
  7. Kunavisarut T et al. (2012) Immunohistochemical analysis based Ep-ICD subcellular localization index (ESLI) is a novel marker for metastatic papillary thyroid microcarcinoma.
    BMC Cancer. 12: 523.
  8. Srivastava G et al. (2014) Nuclear Ep-ICD accumulation predicts aggressive clinical course in early stage breast cancer patients.
    BMC Cancer. 14: 726.
  9. View The Latest Product References
  10. Assi, J. et al. (2015) Nuclear Ep-ICD expression is a predictor of poor prognosis in "low risk" prostate adenocarcinomas.
    PLoS One. 10 (2): e0107586.
  11. Somasundaram, R.T. et al. (2016) Subcellular differential expression of Ep-ICD in oral dysplasia and cancer is associated with disease progression and prognosis.
    BMC Cancer. 16: 486.

Further Reading

  1. Winter, M.J. et al. (2003) The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology.
    Am J Pathol. 163 (6): 2139-48.

Synonyms
Ep-CAM
RRID
AB_324459
UniProt
P16422
Entrez Gene
EPCAM
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0005923 tight junction
GO:0008284 positive regulation of cell proliferation
GO:0016323 basolateral plasma membrane
GO:0016324 apical plasma membrane
GO:0016328 lateral plasma membrane

MCA2162XZ

155403 159567 167105 167108

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