CD326 antibody | HEA-125
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Mouse anti human CD236 antibody, clone HEA-125 recognizes CD326 also known as Adenocarcinoma-associated antigen, Epithelial glycoprotein 314 or Epithelial Cell Adhesion Molecule (Ep-CAM). CD326 is a 314 amino acid ~34 kDa single pass type I transmembrane glycoprotein nearing a single thyroglobulin type-1 domain (UniProt: P16422).
CD326 is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells.
CD326 expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells (Balzar et al. 1999), and de novo expression being seen in dysplastic squamous epithelium (Winter et al. 2003). CD326 has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 (Proca et al. 2000) and Ber-EP4 (Patriarca et al. 2012).
CD326 is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells.
CD326 expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells (Balzar et al. 1999), and de novo expression being seen in dysplastic squamous epithelium (Winter et al. 2003). CD326 has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 (Proca et al. 2000) and Ber-EP4 (Patriarca et al. 2012).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid, ready to use.
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 0.5% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 0.5% Bovine Serum Albumin - Immunogen
- HT29 carcinoma cell line.
- Approx. Protein Concentrations
- 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of a mouse myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P16422
- Entrez Gene
- EPCAM
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0005923 tight junction
- GO:0008284 positive regulation of cell proliferation
- GO:0016323 basolateral plasma membrane
- GO:0016324 apical plasma membrane
- GO:0016328 lateral plasma membrane
- Regulatory
- For research purposes only
Applications of CD326 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Immunofluorescence | |||
| Immunohistology - Frozen | 1 / 20 | ||
| Immunohistology - Paraffin 1 | 1 / 20 | ||
| Immunofluorescence | |||
| Immunohistology - Frozen | Neat | ||
| Immunohistology - Paraffin 1 | Neat |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells or 100ul cell suspension.
- Histology Positive Control Tissue
- Human colon & appendix
- Histology Positive Control Tissue
- Human colon.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
Source Reference
-
Moldenhauer, G. et al. (1987) Epithelium-specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker.
Br J Cancer. 56 (6): 714-21.
References for CD326 antibody
-
F, C. et al. (2010) Interactions between endothelial cells and epithelial cells in a combined cell model of airway mucosa: effects on tight junction permeability.
Exp Lung Res. 36 (1): 1-11. -
Winter, M.J. et al. (2003) The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology.
Am J Pathol. 163 (6): 2139-48. -
Momburg, F. et al. (1987) Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues.
Cancer Res. 47 (11): 2883-91. -
Simon, B. et al. (1990) Epithelial glycoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein.
Proc Natl Acad Sci U S A. 87 (7): 2755-9. -
Kemmner, W. et al. (1992) Separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads.
J Immunol Methods. 147 (2): 197-200. -
Inada, M. et al. (2008) Stage-specific regulation of adhesion molecule expression segregates epithelial stem/progenitor cells in fetal and adult human livers.
Hepatol Int. 2 (1): 50-62.
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