CD326 antibody | HEA-125

Mouse anti Human CD326

Product Type
Monoclonal Antibody
Clone
HEA-125
Isotype
IgG1
Specificity
CD326

Product Code Applications Pack Size List Price Your Price Qty
MCA850G
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SDS Safety Datasheet SDS
C F IF P * 0.1 mg loader
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loader
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Mouse anti human CD236 antibody, clone HEA-125 recognizes CD326 also known as Adenocarcinoma-associated antigen, Epithelial glycoprotein 314 or Epithelial Cell Adhesion Molecule (Ep-CAM). CD326 is a 314 amino acid ~34 kDa single pass type I transmembrane glycoprotein nearing a single thyroglobulin type-1 domain (UniProt: P16422).

CD326 is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells.

CD326 expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells (Balzar et al. 1999), and de novo expression being seen in dysplastic squamous epithelium (Winter et al. 2003). CD326 has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 (Proca et al. 2000) and Ber-EP4 (Patriarca et al. 2012).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
HT29 carcinoma cell line.
Approx. Protein Concentrations
0.1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of a mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Immunofluorescence
Immunohistology - Frozen 1 / 20
Immunohistology - Paraffin 1 1 / 20
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells or 100ul cell suspension.
Histology Positive Control Tissue
Human colon & appendix

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Source Reference

  1. Moldenhauer, G. et al. (1987) Epithelium-specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker.
    Br J Cancer. 56 (6): 714-21.

References for CD326 antibody

  1. Chowdhury, F. et al. (2010) Interactions between endothelial cells and epithelial cells in a combined cell model of airway mucosa: effects on tight junction permeability.
    Exp Lung Res. 36 (1): 1-11.
  2. Winter, M.J. et al. (2003) The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology.
    Am J Pathol. 163 (6): 2139-48.
  3. Momburg, F. et al. (1987) Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues.
    Cancer Res. 47 (11): 2883-91.
  4. Simon, B. et al. (1990) Epithelial glycoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein.
    Proc Natl Acad Sci U S A. 87 (7): 2755-9.
  5. Kemmner, W. et al. (1992) Separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads.
    J Immunol Methods. 147 (2): 197-200.
  6. Inada, M. et al. (2008) Stage-specific regulation of adhesion molecule expression segregates epithelial stem/progenitor cells in fetal and adult human livers.
    Hepatol Int. 2 (1): 50-62.

Immunofluorescence

Immunohistology - Paraffin

Synonyms
Ep-CAM
RRID
AB_322943
UniProt
P16422
Entrez Gene
EPCAM
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0005923 tight junction
GO:0008284 positive regulation of cell proliferation
GO:0016323 basolateral plasma membrane
GO:0016324 apical plasma membrane
GO:0016328 lateral plasma membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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