CD314 antibody | 1D11

Mouse anti Human CD314

Product Type
Monoclonal Antibody
Clone
1D11
Isotype
IgG1
Specificity
CD314

Product Code Applications Pack Size List Price Your Price Qty
MCA2405
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.2 mg loader
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loader
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Mouse anti Human CD314 antibody, clone 1D11 recognizes CD314, also known as natural killer receptor G2 (NKG2D) and as killer cell lectin-like receptor subfamily K, member 1 (KLRK1).

CD314 is a C-type lectin-like activating receptor which is expressed on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. CD314 forms homodimers that signal through an associated DAP10 adaptor protein.

Ligands of CD314 include MICA, MICB and UL16 binding protein (ULBP), which are inducibly expressed. Ligand binding toCD314 results in NK cell activation and potent co-stimulation of effector T cells.

Mouse anti Human CD314 antibody, clone 1D11 is reported to inhibit T cell recognition of MICA (Bauer et al. 1999).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
NKL cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised RBF/DnJ mice were fused with cells of the p3 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD314 antibody

  1. Bauer, S. et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA.
    Science. 285 (5428): 727-9.
  2. Das, H. et al. (2004) Mechanisms of Vdelta1 gammadelta T cell activation by microbial components.
    J Immunol. 172 (11): 6578-86.
  3. Groh, V. et al. (2001) Costimulation of CD8alphabeta T cells by NKG2D via engagement by MIC induced on virus-infected cells.
    Nat Immunol. 2 (3): 255-60.
  4. Jinushi, M. et al. (2003) Autocrine/paracrine IL-15 that is required for type I IFN-mediated dendritic cell expression of MHC class I-related chain A and B is impaired in hepatitis C virus infection.
    J Immunol. 171 (10): 5423-9.
  5. Roberts, A.I. et al. (2001) NKG2D receptors induced by IL-15 costimulate CD28-negative effector CTL in the tissue microenvironment.
    J Immunol. 167: 5527-30.
  6. Holmen, C. et al. (2007) Anti endothelial cell autoantibodies selectively activate SAPK/JNK signalling in Wegener's granulomatosis.
    J Am Soc Nephrol. 18: 2497-508.
  7. Sugita, J. et al. (2010) Differential effects of interleukin-12 and interleukin-15 on expansion of NK cell receptor-expressing CD8+ T cells.
    Ann Hematol. 89: 115-20.
  8. Gumperz, J. et al. (2002) Functionally distinct subsets of CD1d-restricted natural killer T cells revealed by CD1d tetramer staining.
    J Exp Med. 195:625-36.
  9. View The Latest Product References
  10. Wu, J. et al. (2002) T cell antigen receptor engagement and specificity in the recognition of stress-inducible MHC class I-related chains by human epithelial gamma delta T cells.
    J Immunol. 169:1236-40.
  11. Wu, J. et al. (2000) DAP10 and DAP12 form distinct, but functionally cooperative, receptor complexes in natural killer cells.
    J Exp Med. 192:1059-68.
  12. Groh, V. et al. (2003) Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis.
    Proc Natl Acad Sci U S A. 100:9452-7
  13. Voigt, J. et al. (2014) Human natural killer cells acting as phagocytes against Candida albicans and mounting an inflammatory response that modulates neutrophil antifungal activity.
    J Infect Dis. 209 (4): 616-26.
  14. Matzner, P. et al. (2013) Resilience of the immune system in healthy young students to 30-hour sleep deprivation with psychological stress.
    Neuroimmunomodulation. 20: 194-204.
  15. Tahrali, I. et al. (2019) CD3-CD56+ NK cells display an inflammatory profile in RR-MS patients.
    Immunol Lett. Oct 04 [Epub ahead of print].
  16. Tanaka, J. et al. (2012) Expansion of NK cells from cord blood with antileukemic activity using GMP-compliant substances without feeder cells.
    Leukemia. 26 (5): 1149-52.

Further Reading

  1. Groh, V. et al. (2003) Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis.
    Proc Natl Acad Sci U S A. 100 (16): 9452-7.

Immunohistology - Frozen

Synonyms
NKG2D
RRID
AB_567174
UniProt
P26718
Entrez Gene
KLRC4-KLRK1
GO Terms
GO:0005887 integral to plasma membrane
GO:0005529 sugar binding
GO:0030101 natural killer cell activation
GO:0031295 T cell costimulation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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