CD312 antibody | 2A1
Filter by Application:F WB IF Reset
|Mouse anti Human CD312 antibody, clone 2A1 recognizes human EMR2, a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins, which is closely related to CD97. EMR2, also known as CD312, is predominantly expressed on myeloid dendritic cells, monocytes and tissue macrophages. Various isoforms of EMR2 have been documented. The ligand for the largest isoform of EMR2 has recently been identified as chrondroitin sulphate, which binds to the fourth EGF-like module of EMR2. Mouse anti Human CD312 antibody, clone 2A1 recognizes the stalk region of EMR2.|
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/50||1/200|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD312 antibody
Kwakkenbos, M.J. et al. (2002) The human EGF-TM7 family member EMR2 is a heterodimeric receptor expressed on myeloid cells.
J Leukoc Biol. 71 (5): 854-62.
Stacey, M. et al. (2003) The epidermal growth factor-like domains of the human EMR2 receptor mediate cell attachment through chondroitin sulfate glycosaminoglycans.
Blood. 102 (8): 2916-24.
Yona, S. et al. (2008) Ligation of the adhesion-GPCR EMR2 regulates human neutrophil function.
FASEB J. 22 (3): 741-51.
Lin, H.H. et al. (2004) Autocatalytic cleavage of the EMR2 receptor occurs at a conserved G protein-coupled receptor proteolytic site motif.
J Biol Chem. 279 (30): 31823-32.
Huang, Y.S. et al. (2018) Membrane-association of EMR2/ADGRE2-NTF is regulated by site-specific N-glycosylation.
Sci Rep. 8 (1): 4532.
Shankar-Hari, M. et al. (2018) Early PREdiction of sepsis using leukocyte surface biomarkers: the ExPRES-sepsis cohort study.
Intensive Care Med. 44 (11): 1836-48.
Tseng, W.Y. et al. (2013) Increased soluble CD4 in serum of rheumatoid arthritis patients is generated by matrix metalloproteinase (MMP)-like proteinases.
PLoS One. 8 (5): e63963.
Kwakkenbos, M.J. et al. (2004) The EGF-TM7 family: a postgenomic view.
Immunogenetics. 55 (10): 655-66.
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