CD312 antibody | 2A1

Mouse anti Human CD312

Product Type
Monoclonal Antibody
Clone
2A1
Isotype
IgG1
Specificity
CD312

Product Code Applications Pack Size List Price Your Price Qty
MCA2330
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SDS Safety Datasheet SDS
C F IP WB 0.2 mg loader
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loader
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Mouse anti Human CD312 antibody, clone 2A1 recognizes human EMR2, a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins, which is closely related to CD97. EMR2, also known as CD312, is predominantly expressed on myeloid dendritic cells, monocytes and tissue macrophages. Various isoforms of EMR2 have been documented. The ligand for the largest isoform of EMR2 has recently been identified as chrondroitin sulphate, which binds to the fourth EGF-like module of EMR2. Mouse anti Human CD312 antibody, clone 2A1 recognizes the stalk region of EMR2.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunohistology - Frozen 1/50 1/200
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Mouse IgG1 Negative Control

References for CD312 antibody

  1. Kwakkenbos, M.J. et al. (2002) The human EGF-TM7 family member EMR2 is a heterodimeric receptor expressed on myeloid cells.
    J Leukoc Biol. 71 (5): 854-62.
  2. Stacey, M. et al. (2003) The epidermal growth factor-like domains of the human EMR2 receptor mediate cell attachment through chondroitin sulfate glycosaminoglycans.
    Blood. 102 (8): 2916-24.
  3. Yona, S. et al. (2008) Ligation of the adhesion-GPCR EMR2 regulates human neutrophil function.
    FASEB J. 22 (3): 741-51.
  4. Lin, H.H. et al. (2004) Autocatalytic cleavage of the EMR2 receptor occurs at a conserved G protein-coupled receptor proteolytic site motif.
    J Biol Chem. 279 (30): 31823-32.
  5. Huang, Y.S. et al. (2018) Membrane-association of EMR2/ADGRE2-NTF is regulated by site-specific N-glycosylation.
    Sci Rep. 8 (1): 4532.
  6. Shankar-Hari, M. et al. (2018) Early PREdiction of sepsis using leukocyte surface biomarkers: the ExPRES-sepsis cohort study.
    Intensive Care Med. 44 (11): 1836-48.
  7. Tseng, W.Y. et al. (2013) Increased soluble CD4 in serum of rheumatoid arthritis patients is generated by matrix metalloproteinase (MMP)-like proteinases.
    PLoS One. 8 (5): e63963.

Further Reading

  1. Kwakkenbos, M.J. et al. (2004) The EGF-TM7 family: a postgenomic view.
    Immunogenetics. 55 (10): 655-66.

Synonyms
EMR2
RRID
AB_566724
UniProt
Q9UHX3
Entrez Gene
EMR2
GO Terms
GO:0005886 plasma membrane
GO:0005509 calcium ion binding
GO:0016021 integral to membrane
GO:0004930 G-protein coupled receptor activity
GO:0007218 neuropeptide signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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