CD29 antibody | 12G10

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Anti Human CD29 Antibody, clone 12G10 thumbnail image 1

Anti Human CD29 Antibody, clone 12G10 thumbnail image 2

Anti Human CD29 Antibody, clone 12G10 thumbnail image 3

Anti Human CD29 Antibody, clone 12G10 thumbnail image 4

Anti Human CD29 Antibody, clone 12G10 gallery image 1 — Figure A. FITC conjugated Mouse anti Human CD61 (MCA2588F) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated Mouse anti Human CD61 (MCA2588F) and Alexa Fluor 647 conjugated Mouse anti Human CD29 (**MCA2028A647**). All experiments performed on red cell lysed human blood gated on live platelets, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Anti Human CD29 Antibody, clone 12G10 gallery image 2 — Figure A. FITC conjugated Mouse anti Human CD14 (MCA596FITC) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Human CD14 (MCA596F) and RPE conjugated Mouse anti Human CD29 (**MCA2028PE**). All experiments performed on red cell lysed human blood gated on monocytes in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.

Anti Human CD29 Antibody, clone 12G10 gallery image 3 — Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD61 (MCA2588A647) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD61 (MCA2588A647) and RPE conjugated Mouse anti Human CD29 (MCA2028PE). All experiments performed on human blood gated on platelets in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.

Anti Human CD29 Antibody, clone 12G10 gallery image 4 — **Published customer image:**
Mouse anti Human CD28 antibody, clone 12G10 (**MCA2028**) used for the identification of rabbit adipose stromal stem cells by flow cytometry.
**Image caption:**
Rabbit fat pad originate performing ASC *in vitro*. a Photomicrographs of fibroblastoid elements adhering to plastic after adipose tissue digestion (upper left panel) subsequently generating colonies (upper right panel) visualized by Crystal Violet staining (lower left panel) and reaching confluence after three passages (lower right panel Ph1, scale bar 100 μm). b Rabbit ASC cumulative population doubling after passage 3(P3). c Mean values ± SEM of antigen expressed on rabbit ASC by FACS (left table); representative immunophenotypical characterizations of rabbit ASC with cells positive for CD29, CD49e and CD90 (isotype control in gray). d Adipogenic differentiation after 10 days of induction visualized by Oil Red O staining and a corresponding non-induced control (upper panels; scale bar 100 μm). Representative osteogenic differentiation visualized by Alizarin Red staining after 14 days of induction and a corresponding non-induced control (lower panels)

From: Piccinno MS, Veronesi E, Loschi P, Pignatti M, Murgia A, Grisendi G, Castelli I, Bernabei D, Candini O, Conte P, Paolucci P, Horwitz EM, De Santis G, Iughetti L, Dominici M.
Adipose stromal/stem cells assist fat transplantation reducing necrosis and increasing graft performance.
Apoptosis. 2013 Oct;18(10):1274-89.

Mouse anti Human CD29:Alexa Fluor® 647

Mouse anti Human CD29:FITC

Mouse anti Human CD29

Mouse anti Human CD29:RPE

Product Type: Monoclonal Antibody
Clone: 12G10
Isotype: IgG1

Product Code	Applications	Pack Size
MCA2028A647	F	100 Tests/1ml
MCA2028F	F	0.1 mg
MCA2028T	C E F IF IP WB	25 µg
MCA2028	C E F IF IP WB	0.2 mg
MCA2028PE	F	100 Tests

Mouse anti Human CD29 monoclonal antibody, clone 12G10 recognizes human CD29 also known as beta1 integrin or VLA-4 subunit alpha. CD29 is a ~130 kDa under reducing, ~115 kDa under non-reducing conditions single pass type I transmembrane glycoprotein. CD29 acts as the common beta subunit of the heterodimeric very late antigens 1-6, complexing with CD49a-f respectively where it forms part of the receptors for laminin , collagen and fibronectin. the VLA heterodimers mediate cell-cell and cell-matrix interactions.

Mouse anti Human CD29, clone 12G10 binding to cells adhering via VLA-4 results in actin cytoskeletal disruption and subsequent inhibition of attachment and spreading whilst 12G10 binding to cells adhering via VLA-5 results in enhancement of both these processes (Humphries et al. 2005). Clone 12G10 enhances alpha 5 beta 1-fibronectin interactions and binds to a region of CD25 containing the binding epitopes of several other anti CD29 antibody clones. However, unlike these, binding of 12G10 is enhanced in the presence of ligands such as fibronectin fragments (Mould et al. 1995). Binding of antibody clone 12G10 to the integrin β1 subunit is affected by divalent cations and the binding epitope appears to be located around residues 207-218 in the b1 subunit putative A-domain (Mould et al. 1998).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat
Mouse
Mink

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Alexa Fluor 647 - liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose

Carrier Free

Yes

Immunogen

Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from an immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Shelf Life: 12 months from date of despatch
Shelf Life: 18 months from date of despatch
Shelf Life: 18 months from date of despatch.
Shelf Life: 12 months from date of reconstitution

More Information

UniProt: P05556 Related reagents
Entrez Gene: ITGB1 Related reagents
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of CD29 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/2
Flow Cytometry	Neat
ELISA		10ug/ml
Flow Cytometry	1/25	1/50
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Flow Cytometry	Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:Alexa Fluor® 647	MCA928A647	100 Tests/1ml	F
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Application Based External Images

Functional Assays

Immunofluorescence

Product Specific References

References for CD29 antibody

Sodek, K.L. et al. (2009) Compact spheroid formation by ovarian cancer cells is associated with contractile behavior and an invasive phenotype.
Int J Cancer. 124: 2060-70.
Mould, A.P. et al. (1995) Regulation of integrin alpha 5 beta 1 function by anti-integrin antibodies and divalent cations.
Biochem Soc Trans. 23 (3): 395S.
Mould, A.P. et al. (1995) Identification of a novel anti-integrin monoclonal antibody that recognises a ligand-induced binding site epitope on the beta 1 subunit.
FEBS Lett. 363 (1-2): 118-22.
Matthews,B.D. et al. (2010) Ultra-rapid activation of TRPV4 ion channels by mechanical forces applied to cell surface [beta]1 integrins.
Integr Biol (Camb). 2: 435-42.
Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
Vet Immunol Immunopathol. 119: 27-37.
Kawaguchi, N. et al. (2003) ADAM12 induces actin cytoskeleton and extracellular matrix reorganization during early adipocyte differentiation by regulating beta1 integrin function.
J Cell Sci. 116: 3893-904.
Loughran, G. et al. (2005) Mystique is a new insulin-like growth factor-I-regulated PDZ-LIM domain protein that promotes cell attachment and migration and suppresses Anchorage-independent growth.
Mol Biol Cell. 2005 Apr;16(4):1811-22.
Werner, J. et al. (2012) Expression of integrins and Toll-like receptors in cervical cancer: Effect of infectious agents.
Innate Immun. 18: 55-69.
Iba, K. et al. (2000) The cysteine-rich domain of human ADAM 12 supports cell adhesion through syndecans and triggers signaling events that lead to beta1 integrin-dependent cell spreading.
J Cell Biol. 149: 1143-56.
Meng, X. et al. (2005) Evidence for the presence of a low-mass beta1 integrin on the cell surface.
J Cell Sci. 118: 4009-16.
Whittard, J.D. and Akiyama, S.K. (2001) Positive regulation of cell-cell and cell-substrate adhesion by protein kinase A.
J Cell Sci. 114: 3265-72.
Rodriguez-Teja, M. et al. (2015) AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival.
J Pathol. 235 (4): 581-92.
Zhong, C. et al. (1998) Rho-mediated contractility exposes a cryptic site in fibronectin and induces fibronectin matrix assembly.
J Cell Biol. 141: 539-51.
Zhou, J. et al. (2008) Salvicine inactivates beta 1 integrin and inhibits adhesion of MDA-MB-435 cells to fibronectin via reactive oxygen species signaling.
Mol Cancer Res. 6: 194-204.
Thodeti, C.K. et al. (2003) ADAM12/syndecan-4 signaling promotes beta 1 integrin-dependent cell spreading through protein kinase Calpha and RhoA.
J Biol Chem. 278: 9576-84.
Lee, H. et al. (2006) A critical role for the membrane-type 1 matrix metalloproteinase in collagen phagocytosis.
Mol Biol Cell. 17: 4812-26.
Gravelle, S. et al. (2010) Up-regulation of integrin expression in lung adenocarcinoma cells caused by bacterial infection: in vitro study.
Innate Immun. 2010 Feb;16(1):14-26.
Jović, M. et al. (2007) EHD1 regulates beta1 integrin endosomal transport: effects on focal adhesions, cell spreading and migration.
J Cell Sci. 120: 802-14.
Newsome, P.N. et al. (2014) Serum from patients with fulminant hepatic failure causes hepatocyte detachment and apoptosis by a beta(1)-integrin pathway.
Hepatology. 40: 636-45.
Piccinno, M.S. et al. (2013) Adipose stromal/stem cells assist fat transplantation reducing necrosis and increasing graft performance.
Apoptosis. 18 (10): 1274-89.

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