CD29 antibody | 12G10

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Mouse anti Human CD29:Alexa Fluor® 647

Mouse anti Human CD29:FITC

Mouse anti Human CD29

Mouse anti Human CD29:RPE

Product Type
Monoclonal Antibody
Clone
12G10
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2028A647 F 100 Tests/1ml
MCA2028F F 0.1 mg
MCA2028T C E F IF IP WB 25 µg
MCA2028 C E F IF IP WB 0.2 mg
MCA2028PE F 100 Tests
Mouse anti Human CD29 monoclonal antibody, clone 12G10 recognizes human CD29 also known as beta1 integrin or VLA-4 subunit alpha. CD29 is a ~130 kDa under reducing, ~115 kDa under non-reducing conditions single pass type I transmembrane glycoprotein. CD29 acts as the common beta subunit of the heterodimeric very late antigens 1-6, complexing with CD49a-f respectively where it forms part of the receptors for laminin , collagen and fibronectin. the VLA heterodimers mediate cell-cell and cell-matrix interactions.

Mouse anti Human CD29, clone 12G10 binding to cells adhering via VLA-4 results in actin cytoskeletal disruption and subsequent inhibition of attachment and spreading whilst 12G10 binding to cells adhering via VLA-5 results in enhancement of both these processes (Humphries et al. 2005). Clone 12G10 enhances alpha 5 beta 1-fibronectin interactions and binds to a region of CD25 containing the binding epitopes of several other anti CD29 antibody clones. However, unlike these, binding of 12G10 is enhanced in the presence of ligands such as fibronectin fragments (Mould et al. 1995). Binding of antibody clone 12G10 to the integrin β1 subunit is affected by divalent cations and the binding epitope appears to be located around residues 207-218 in the b1 subunit putative A-domain (Mould et al. 1998).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
Mink
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from an immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Shelf Life
12 months from date of despatch
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution

More Information

UniProt
P05556 Related reagents
Entrez Gene
ITGB1 Related reagents
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD29 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/2
Flow Cytometry Neat
ELISA 10ug/ml
Flow Cytometry 1/25 1/50
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 100 Tests/1ml F
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Functional Assays

Immunofluorescence

Product Specific References

References for CD29 antibody

  1. Sodek, K.L. et al. (2009) Compact spheroid formation by ovarian cancer cells is associated with contractile behavior and an invasive phenotype.
    Int J Cancer. 124: 2060-70.
  2. Mould, A.P. et al. (1995) Regulation of integrin alpha 5 beta 1 function by anti-integrin antibodies and divalent cations.
    Biochem Soc Trans. 23 (3): 395S.
  3. Mould, A.P. et al. (1995) Identification of a novel anti-integrin monoclonal antibody that recognises a ligand-induced binding site epitope on the beta 1 subunit.
    FEBS Lett. 363 (1-2): 118-22.
  4. Matthews,B.D. et al. (2010) Ultra-rapid activation of TRPV4 ion channels by mechanical forces applied to cell surface [beta]1 integrins.
    Integr Biol (Camb). 2: 435-42.
  5. Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
    Vet Immunol Immunopathol. 119: 27-37.
  6. Kawaguchi, N. et al. (2003) ADAM12 induces actin cytoskeleton and extracellular matrix reorganization during early adipocyte differentiation by regulating beta1 integrin function.
    J Cell Sci. 116: 3893-904.
  7. Loughran, G. et al. (2005) Mystique is a new insulin-like growth factor-I-regulated PDZ-LIM domain protein that promotes cell attachment and migration and suppresses Anchorage-independent growth.
    Mol Biol Cell. 2005 Apr;16(4):1811-22.
  8. Werner, J. et al. (2012) Expression of integrins and Toll-like receptors in cervical cancer: Effect of infectious agents.
    Innate Immun. 18: 55-69.
  9. Iba, K. et al. (2000) The cysteine-rich domain of human ADAM 12 supports cell adhesion through syndecans and triggers signaling events that lead to beta1 integrin-dependent cell spreading.
    J Cell Biol. 149: 1143-56.
  10. Meng, X. et al. (2005) Evidence for the presence of a low-mass beta1 integrin on the cell surface.
    J Cell Sci. 118: 4009-16.
  11. Whittard, J.D. and Akiyama, S.K. (2001) Positive regulation of cell-cell and cell-substrate adhesion by protein kinase A.
    J Cell Sci. 114: 3265-72.
  12. Rodriguez-Teja, M. et al. (2015) AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival.
    J Pathol. 235 (4): 581-92.
  13. Zhong, C. et al. (1998) Rho-mediated contractility exposes a cryptic site in fibronectin and induces fibronectin matrix assembly.
    J Cell Biol. 141: 539-51.
  14. Zhou, J. et al. (2008) Salvicine inactivates beta 1 integrin and inhibits adhesion of MDA-MB-435 cells to fibronectin via reactive oxygen species signaling.
    Mol Cancer Res. 6: 194-204.
  15. Thodeti, C.K. et al. (2003) ADAM12/syndecan-4 signaling promotes beta 1 integrin-dependent cell spreading through protein kinase Calpha and RhoA.
    J Biol Chem. 278: 9576-84.
  16. Lee, H. et al. (2006) A critical role for the membrane-type 1 matrix metalloproteinase in collagen phagocytosis.
    Mol Biol Cell. 17: 4812-26.
  17. Gravelle, S. et al. (2010) Up-regulation of integrin expression in lung adenocarcinoma cells caused by bacterial infection: in vitro study.
    Innate Immun. 2010 Feb;16(1):14-26.
  18. Jović, M. et al. (2007) EHD1 regulates beta1 integrin endosomal transport: effects on focal adhesions, cell spreading and migration.
    J Cell Sci. 120: 802-14.
  19. Newsome, P.N. et al. (2014) Serum from patients with fulminant hepatic failure causes hepatocyte detachment and apoptosis by a beta(1)-integrin pathway.
    Hepatology. 40: 636-45.
  20. Piccinno, M.S. et al. (2013) Adipose stromal/stem cells assist fat transplantation reducing necrosis and increasing graft performance.
    Apoptosis. 18 (10): 1274-89.