CD262 antibody | DJR2-4

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Anti Human CD262 Antibody, clone DJR2-4 gallery image 1 — BJAB cells stained with Mouse anti Human CD262 (MCA4638T):green. Image courtesy of Dr C. Gillet. University of Strasbourg

Mouse anti Human CD262

Product Type: Monoclonal Antibody
Clone: DJR2-4
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA4638T	F IF			25 µg

Mouse anti Human CD262 antibody, clone DJR2-4recognises human CD262, also known as Death receptor 5 (DR5) and TNF-related apoptosis-inducing ligand receptor 2 (TRAIL-R2). It is a ~55 kDa member of the TNF receptor superfamily binding tumor necrosis factor-related apoptosis inducing ligand (TNFSF10, TRAIL or APO-2L). Once activated, this receptor induces a cascade of caspases resulting in apoptosis. FADD, a death domain containing adaptor protein is required for apoptosis mediated by DR5. DR5 is widely expressed in adult and fetal tissues. Staining may be enhanced with the use of a sensitive, three-layer staining technique.

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Immunogen

Extracellular domain of DR5-human IgG1 Fc fusion protein.

Approx. Protein Concentrations

IgG concentration 0.5mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: O14763 Related reagents
Entrez Gene: TNFRSF10B Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0016021 integral to membrane; GO:0004872 receptor activity; GO:0006919 activation of caspase activity; GO:0007166 cell surface receptor linked signaling pathway; GO:0007250 activation of NF-kappaB-inducing kinase activity; GO:0008625 induction of apoptosis via death domain receptors; GO:0008633 activation of pro-apoptotic gene products; GO:0008656 caspase activator activity; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade; GO:0045569 TRAIL binding
Regulatory: For research purposes only

Applications of CD262 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		1/10	1/20
Immunofluorescence

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F