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CD262 antibody | DJR2-4

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Anti Human CD262 Antibody, clone DJR2-4 gallery image 1
BJAB cells stained with Mouse anti Human CD262 (MCA4638T):green. Image courtesy of Dr C. Gillet. University of Strasbourg

Mouse anti Human CD262

Product Type
Monoclonal Antibody
Clone
DJR2-4
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA4638T
Datasheet
F IF
SDS
25 µg loader

Mouse anti Human CD262 antibody, clone DJR2-4recognises human CD262, also known as Death receptor 5 (DR5) and TNF-related apoptosis-inducing ligand receptor 2 (TRAIL-R2). It is a ~55 kDa member of the TNF receptor superfamily binding tumor necrosis factor-related apoptosis inducing ligand (TNFSF10, TRAIL or APO-2L). Once activated, this receptor induces a cascade of caspases resulting in apoptosis. FADD, a death domain containing adaptor protein is required for apoptosis mediated by DR5. DR5 is widely expressed in adult and fetal tissues. Staining may be enhanced with the use of a sensitive, three-layer staining technique.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Extracellular domain of DR5-human IgG1 Fc fusion protein.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O14763
Entrez Gene
TNFRSF10B
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0006919 activation of caspase activity
GO:0007166 cell surface receptor linked signaling pathway
GO:0007250 activation of NF-kappaB-inducing kinase activity
GO:0008625 induction of apoptosis via death domain receptors
GO:0008633 activation of pro-apoptotic gene products
GO:0008656 caspase activator activity
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0045569 TRAIL binding
Regulatory
For research purposes only

Applications of CD262 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/20
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

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Apoptosis Resources

Apoptosis Phases

Apoptosis Webinar

Apoptosis Kits

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