CD262 antibody | DJR2-4
Mouse anti Human CD262 antibody, clone DJR2-4recognises human CD262, also known as Death receptor 5 (DR5) and TNF-related apoptosis-inducing ligand receptor 2 (TRAIL-R2). It is a ~55 kDa member of the TNF receptor superfamily binding tumor necrosis factor-related apoptosis inducing ligand (TNFSF10, TRAIL or APO-2L). Once activated, this receptor induces a cascade of caspases resulting in apoptosis. FADD, a death domain containing adaptor protein is required for apoptosis mediated by DR5. DR5 is widely expressed in adult and fetal tissues. Staining may be enhanced with the use of a sensitive, three-layer staining technique.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Extracellular domain of DR5-human IgG1 Fc fusion protein.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- O14763
- Entrez Gene
- TNFRSF10B
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0006919 activation of caspase activity
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0007250 activation of NF-kappaB-inducing kinase activity
- GO:0008625 induction of apoptosis via death domain receptors
- GO:0008633 activation of pro-apoptotic gene products
- GO:0008656 caspase activator activity
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0045569 TRAIL binding
- Regulatory
- For research purposes only
Applications of CD262 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/10 | 1/20 | |
| Immunofluorescence |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
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