CD26 antibody | M-A261

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Anti Human CD26 Antibody, clone M-A261 thumbnail image 1

Anti Human CD26 Antibody, clone M-A261 thumbnail image 2

Anti Human CD26 Antibody, clone M-A261 gallery image 1 — Figure A. RPE conjugated mouse anti human CD19 (MCA1940PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti human CD19 (MCA1940PE) and FITC conjugated mouse anti human CD26 (**MCA1317F**). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.

Anti Human CD26 Antibody, clone M-A261 gallery image 2 — Figure A. Alexa Fluor 488 conjugated mouse anti human CD19 (MCA1940A488) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. Alexa Fluor 488 conjugated mouse anti human CD19 (MCA1940A488) and RPE conjugated mouse anti human CD26 (**MCA1317PE**). All experiments performed on human peripheral blood lymphocytes in the presence of human seroblock (BUF070A).

Mouse anti Human CD26:FITC

Mouse anti Human CD26

Mouse anti Human CD26:RPE

Product Type: Monoclonal Antibody
Clone: M-A261
Isotype: IgG1

Product Code	Applications	Pack Size
MCA1317F	F	0.1 mg
MCA1317T	C* F IP	25 µg
MCA1317GA	C* F IP	0.1 mg
MCA1317	C* F IP	0.2 mg
MCA1317PE	F	100 Tests

Mouse anti Human CD26 antibody, clone M-A261, recognizes human dipeptidyl peptidase 4, also known as adenosine deaminase complexing protein 2, CD26, TP103, ADABP or DPP IV. CD26 is a 766 amino acid ~110 kDa single pass type II transmembrane glycoprotein expressed by activated T cells, B cells and macrophages. A soluble form of CD26 can be derived by cleavage of the membrane bound form between residues 38-39 (Uniprot: 27487).

CD26 acts as a functional receptor for the pathogenic Middle East respiratory syndrome coronavirus [MERS-CoV] (Raj et al. 2014), a severe and frequently fatal disease in humans and marmosets sharing identical CD26 sequence at the site of interaction with the MERS-CoV spike protein (Lu et al. 2013, Wang et al. 2013).

Mouse anti Human CD26 antibody, clone M-A261 has been used successfully for the immunohistochemical detection of CD26 on formalin fixed, paraffin embedded tissues and the demonstration of elevated CD26 expression in thyroid neoplasia (Kholová et al. 2003). Mouse anti Human CD26 antibody, clone M-A261 has also been used for the immunohistochemical detection of CD26 on cryostat sections using both immunoperoxidase and immunofluorescence staining in liver biopsies from NASH patients (Balaban et al. 2007).

Product Details

Target Species

Human

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Reconstitute with 1ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Carrier Free

Yes

Immunogen

Human T-cell leukemia cells (T-CLL)

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch
Shelf Life: 18 months from date of despatch.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: P27487 Related reagents
Entrez Gene: DPP4 Related reagents
GO Terms: GO:0001666 response to hypoxia; GO:0002020 protease binding; GO:0004252 serine-type endopeptidase activity; GO:0004177 aminopeptidase activity; GO:0008239 dipeptidyl-peptidase activity; GO:0005102 receptor binding; GO:0007155 cell adhesion; GO:0016021 integral to membrane; GO:0005576 extracellular region; GO:0004872 receptor activity; GO:0030139 endocytic vesicle; GO:0006508 proteolysis; GO:0008284 positive regulation of cell proliferation; GO:0009986 cell surface; GO:0010716 negative regulation of extracellular matrix disassembly; GO:0016324 apical plasma membrane; GO:0031258 lamellipodium membrane; GO:0031295 T cell costimulation; GO:0033632 regulation of cell-cell adhesion mediated by integrin; GO:0042110 T cell activation; GO:0042803 protein homodimerization activity; GO:0043542 endothelial cell migration; GO:0045121 membrane raft; GO:0071438 invadopodium membrane
Regulatory: For research purposes only

Applications of CD26 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry		Neat
Flow Cytometry	1/50	1/100
Immunohistology - Frozen ¹
Immunohistology - Paraffin
Immunoprecipitation
Flow Cytometry		Neat

¹The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells or 100ul whole blood
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells or 100ul whole blood
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells or 100ul whole blood
Histology Positive Control Tissue: Thymus

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Application Based External Images

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Immunoprecipitation

Product Specific References

References for CD26 antibody

Berg, L.P. et al. (2002) Functional consequences of noncognate interactions between CD4+ memory T lymphocytes and the endothelium.
J Immunol. 168 (7): 3227-34.
Post, S. et al. (2010) Impaired recruitment of HHT-1 mononuclear cells to the ischaemic heart is due to an altered CXCR4/CD26 balance.
Cardiovasc Res. 85: 494-502.
Balaban, Y.H. et al. (2007) Dipeptidyl peptidase IV (DDP IV) in NASH patients.
Ann Hepatol. 6: 242-50.
Krijnen, P.A. et al. (2012) Loss of DPP4 activity is related to a prothrombogenic status of endothelial cells: implications for the coronary microvasculature of myocardial infarction patients.
Basic Res Cardiol. 107: 233.
Kholová, I. et al. (2003) Immunohistochemical detection of dipeptidyl peptidase IV (CD 26) in thyroid neoplasia using biotinylated tyramine amplification.
Neoplasma. 50: 159-64.
Lutz, M.S. and Burk, R.D. (2006) Primary cilium formation requires von hippel-lindau gene function in renal-derived cells.
Cancer Res. 66: 6903-7.
James, M.J. (2003) Anergic T cells exert antigen-independent inhibition of cell-cell interactions via chemokine metabolism.
Blood.102: 2173-9.
Snooks, M.J. et al. (2008) Vectorial entry and release of hepatitis A virus in polarized human hepatocytes.
J Virol. 82: 8733-42.
Sun, A.L. et al. (2012) Dipeptidyl peptidase-IV is a potential molecular biomarker in diabetic kidney disease.
Diab Vasc Dis Res. 9: 301-8.
Le Naour, F. et al. (2006) Profiling of the tetraspanin web of human colon cancer cells.
Mol Cell Proteomics. 5: 845-57.
Akilov, O.E. et al. (2012) Resistance of Sézary cells to TNF-α-induced apoptosis is mediated in part by a loss of TNFR1 and a high level of the IER3 expression.
Exp Dermatol. 21: 287-92.

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