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CD26 antibody | M-A261

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Anti Human CD26 Antibody, clone M-A261 thumbnail image 1 Anti Human CD26 Antibody, clone M-A261 thumbnail image 2
Anti Human CD26 Antibody, clone M-A261 gallery image 1
Figure A. RPE conjugated mouse anti human CD19 (MCA1940PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti human CD19 (MCA1940PE) and FITC conjugated mouse anti human CD26 (MCA1317F). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Human CD26 Antibody, clone M-A261 gallery image 2
Figure A. Alexa Fluor 488 conjugated mouse anti human CD19 (MCA1940A488) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. Alexa Fluor 488 conjugated mouse anti human CD19 (MCA1940A488) and RPE conjugated mouse anti human CD26 (MCA1317PE). All experiments performed on human peripheral blood lymphocytes in the presence of human seroblock (BUF070A).

Mouse anti Human CD26:FITC

Mouse anti Human CD26

Mouse anti Human CD26:RPE

Product Type
Monoclonal Antibody
Clone
M-A261
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1317F
Datasheet
F
SDS
0.1 mg loader

MCA1317GA
Datasheet
C* F IP
SDS
0.1 mg loader

MCA1317
Datasheet
C* F IP
SDS
0.2 mg loader

MCA1317T
Datasheet
C* F IP
SDS
25 µg loader

MCA1317PE
Datasheet
F
SDS
100 Tests loader

Mouse anti Human CD26 antibody, clone M-A261, recognizes human dipeptidyl peptidase 4, also known as adenosine deaminase complexing protein 2, CD26, TP103, ADABP or DPP IV. CD26 is a 766 amino acid ~110 kDa single pass type II transmembrane glycoprotein expressed by activated T cells, B cells and macrophages. A soluble form of CD26 can be derived by cleavage of the membrane bound form between residues 38-39 (Uniprot: 27487).

CD26 acts as a functional receptor for the pathogenic Middle East respiratory syndrome coronavirus [MERS-CoV] (Raj et al. 2014), a severe and frequently fatal disease in humans and marmosets sharing identical CD26 sequence at the site of interaction with the MERS-CoV spike protein (Lu et al. 2013, Wang et al. 2013).

Mouse anti Human CD26 antibody, clone M-A261 has been used successfully for the immunohistochemical detection of CD26 on formalin fixed, paraffin embedded tissues and the demonstration of elevated CD26 expression in thyroid neoplasia (Kholová et al. 2003). Mouse anti Human CD26 antibody, clone M-A261 has also been used for the immunohistochemical detection of CD26 on cryostat sections using both immunoperoxidase and immunofluorescence staining in liver biopsies from NASH patients (Balaban et al. 2007).

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
Human T-cell leukemia cells (T-CLL)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P27487
Entrez Gene
DPP4
GO Terms
GO:0001666 response to hypoxia
GO:0002020 protease binding
GO:0004252 serine-type endopeptidase activity
GO:0004177 aminopeptidase activity
GO:0008239 dipeptidyl-peptidase activity
GO:0005102 receptor binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0004872 receptor activity
GO:0030139 endocytic vesicle
GO:0006508 proteolysis
GO:0008284 positive regulation of cell proliferation
GO:0009986 cell surface
GO:0010716 negative regulation of extracellular matrix disassembly
GO:0016324 apical plasma membrane
GO:0031258 lamellipodium membrane
GO:0031295 T cell costimulation
GO:0033632 regulation of cell-cell adhesion mediated by integrin
GO:0042110 T cell activation
GO:0042803 protein homodimerization activity
GO:0043542 endothelial cell migration
GO:0045121 membrane raft
GO:0071438 invadopodium membrane
Regulatory
For research purposes only

Applications of CD26 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/50 1/100
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunoprecipitation
Flow Cytometry Neat
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Histology Positive Control Tissue
Thymus
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Immunoprecipitation

Product Specific References

References for CD26 antibody

  1. Berg, L.P. et al. (2002) Functional consequences of noncognate interactions between CD4+ memory T lymphocytes and the endothelium.
    J Immunol. 168 (7): 3227-34.
  2. Post, S. et al. (2010) Impaired recruitment of HHT-1 mononuclear cells to the ischaemic heart is due to an altered CXCR4/CD26 balance.
    Cardiovasc Res. 85: 494-502.
  3. Balaban, Y.H. et al. (2007) Dipeptidyl peptidase IV (DDP IV) in NASH patients.
    Ann Hepatol. 6: 242-50.
  4. Krijnen, P.A. et al. (2012) Loss of DPP4 activity is related to a prothrombogenic status of endothelial cells: implications for the coronary microvasculature of myocardial infarction patients.
    Basic Res Cardiol. 107: 233.
  5. Kholová, I. et al. (2003) Immunohistochemical detection of dipeptidyl peptidase IV (CD 26) in thyroid neoplasia using biotinylated tyramine amplification.
    Neoplasma. 50: 159-64.
  6. Lutz, M.S. and Burk, R.D. (2006) Primary cilium formation requires von hippel-lindau gene function in renal-derived cells.
    Cancer Res. 66: 6903-7.
  7. James, M.J. (2003) Anergic T cells exert antigen-independent inhibition of cell-cell interactions via chemokine metabolism.
    Blood.102: 2173-9.
  8. Snooks, M.J. et al. (2008) Vectorial entry and release of hepatitis A virus in polarized human hepatocytes.
    J Virol. 82: 8733-42.
  9. Sun, A.L. et al. (2012) Dipeptidyl peptidase-IV is a potential molecular biomarker in diabetic kidney disease.
    Diab Vasc Dis Res. 9: 301-8.
  10. Le Naour, F. et al. (2006) Profiling of the tetraspanin web of human colon cancer cells.
    Mol Cell Proteomics. 5: 845-57.
  11. Akilov, O.E. et al. (2012) Resistance of Sézary cells to TNF-α-induced apoptosis is mediated in part by a loss of TNFR1 and a high level of the IER3 expression.
    Exp Dermatol. 21: 287-92.

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