CD253 antibody | 2E5

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Mouse anti Human CD253:Low Endotoxin

Product Type: Monoclonal Antibody
Clone: 2E5
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2144ELGA	F FN WB			0.1 mg
MCA2144EL	F FN WB			0.5 mg

Mouse anti Human CD253 antibody, clone 2E5 recognizes the 281 amino acid protein termed TNF related apoptosis-inducing ligand (TRAIL), also known as Apo2L.

TRAIL is a cytotoxic protein closely related to Fas (CD95), which induces extensive apoptosis in a range of cell types. TRAIL induces apoptosis via the receptors DR4 and DR5. Most normal tissues are resistant to TRAIL induced apoptosis due to their expression of decoy receptors, DcR1 and DcR2, which antagonise TRAIL activity.

TRAIL is expressed by cell lines, activated lymphocytes and on syncytiotrophoblast cells in first trimester pregnancy.

This antibody neutralizes TRAIL activity.

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Immunogen: Recombinant human CD253 (TRAIL) aa 95-281.
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Endotoxin Level: <0.01EU/ug

Storage Information

Storage: Pack Size: 0.1 mg
Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

Pack Size: 0.5 mg
Store at -20^oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P50591 Related reagents
Entrez Gene: TNFSF10 Related reagents
GO Terms: GO:0007165 signal transduction; GO:0005887 integral to plasma membrane; GO:0005125 cytokine activity; GO:0005164 tumor necrosis factor receptor binding; GO:0046872 metal ion binding; GO:0005615 extracellular space; GO:0005625 soluble fraction; GO:0006919 activation of caspase activity; GO:0006955 immune response; GO:0007267 cell-cell signaling; GO:0008624 induction of apoptosis by extracellular signals; GO:0008633 activation of pro-apoptotic gene products; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
Regulatory: For research purposes only

Applications of CD253 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry
Functional Assays
Western Blotting

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control:Low Endotoxin	MCA928EL	0.5 mg	C F

Product Specific References

References for CD253 antibody

Plasilova, M. et al. (2002) TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors.
Leukemia. 16: 67-73.

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