CD253 antibody | 2E5

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Mouse anti Human CD253:Low Endotoxin

Product Type
Monoclonal Antibody
Clone
2E5
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2144ELGA F FN WB 0.1 mg
MCA2144EL F FN WB 0.5 mg
Mouse anti Human CD253 antibody, clone 2E5 recognizes the 281 amino acid protein termed TNF related apoptosis-inducing ligand (TRAIL), also known as Apo2L.

TRAIL is a cytotoxic protein closely related to Fas (CD95), which induces extensive apoptosis in a range of cell types. TRAIL induces apoptosis via the receptors DR4 and DR5. Most normal tissues are resistant to TRAIL induced apoptosis due to their expression of decoy receptors, DcR1 and DcR2, which antagonise TRAIL activity.

TRAIL is expressed by cell lines, activated lymphocytes and on syncytiotrophoblast cells in first trimester pregnancy.

This antibody neutralizes TRAIL activity.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant human CD253 (TRAIL) aa 95-281.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Endotoxin Level
<0.01EU/ug

Storage Information

Storage
Pack Size: 0.1 mg
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.5 mg
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P50591 Related reagents
Entrez Gene
TNFSF10 Related reagents
GO Terms
GO:0007165 signal transduction
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0046872 metal ion binding
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006919 activation of caspase activity
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0008624 induction of apoptosis by extracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
Regulatory
For research purposes only

Applications of CD253 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Functional Assays
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:Low Endotoxin MCA928EL 0.5 mg C F

Product Specific References

References for CD253 antibody

  1. Plasilova, M. et al. (2002) TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors.
    Leukemia. 16: 67-73.