CD253 antibody | 2E5
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Mouse anti Human CD253 antibody, clone 2E5 recognizes the 281 amino acid protein termed TNF related apoptosis-inducing ligand (TRAIL), also known as Apo2L.
TRAIL is a cytotoxic protein closely related to Fas (CD95), which induces extensive apoptosis in a range of cell types. TRAIL induces apoptosis via the receptors DR4 and DR5. Most normal tissues are resistant to TRAIL induced apoptosis due to their expression of decoy receptors, DcR1 and DcR2, which antagonise TRAIL activity.
TRAIL is expressed by cell lines, activated lymphocytes and on syncytiotrophoblast cells in first trimester pregnancy.
This antibody neutralizes TRAIL activity.
TRAIL is a cytotoxic protein closely related to Fas (CD95), which induces extensive apoptosis in a range of cell types. TRAIL induces apoptosis via the receptors DR4 and DR5. Most normal tissues are resistant to TRAIL induced apoptosis due to their expression of decoy receptors, DcR1 and DcR2, which antagonise TRAIL activity.
TRAIL is expressed by cell lines, activated lymphocytes and on syncytiotrophoblast cells in first trimester pregnancy.
This antibody neutralizes TRAIL activity.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant human CD253 (TRAIL) aa 95-281.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Endotoxin Level
- < 0.01 EU/ug
Storage Information
- Storage
- Pack Size: 0.1 mgStore at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.5 mgStore at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- P50591
- Entrez Gene
- TNFSF10
- GO Terms
- GO:0007165 signal transduction
- GO:0005887 integral to plasma membrane
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0046872 metal ion binding
- GO:0005615 extracellular space
- GO:0005625 soluble fraction
- GO:0006919 activation of caspase activity
- GO:0006955 immune response
- GO:0007267 cell-cell signaling
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0008633 activation of pro-apoptotic gene products
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- Regulatory
- For research purposes only
Applications of CD253 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Functional Assays | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Low Endotoxin | MCA928EL | C F | 0.5 mg |
|
Product Specific References
References for CD253 antibody
-
Plasilova, M. et al. (2002) TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors.
Leukemia. 16: 67-73.
