Mouse anti Human CD236 antibody, clone BRIC4 recognizes the erythrocyte transmembrane glycoprotein CD236, also known as glycophorin C (GPC). CD236 is an integral membrane protein which carries the Gerbich blood group antigens. Expression of CD236 is not restricted to erythroid cells, but is broadly distributed in both erythroid and non-erythroid tissues.
Mouse anti Human CD236 antibody, clone BRIC4 specifically reacts with an epitope located within residues 2-21 of CD236.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Human erythrocytes.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to plasma membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD236 antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for CD236 antibody
Anstee, D.J .et al. (1984) Two individuals with elliptocytic red cells apparently lack three minor erythrocyte membrane sialoglycoproteins.
Biochem J. 218 (2): 615-9.
Dahr. W/ et al. (1989) High-frequency antigens of human erythrocyte membrane sialoglycoproteins. VI. Monoclonal antibodies reacting with the N-terminal domain of glycophorin C.
Biol Chem Hoppe Seyler. 370 (8): 849-54.
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