CD236 antibody | BRIC4

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Mouse anti Human CD236

Product Type
Monoclonal Antibody
Clone
BRIC4
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1986 C F H WB 0.2 mg
Mouse anti Human CD236 antibody, clone BRIC4 recognizes the erythrocyte transmembrane glycoprotein CD236, also known as glycophorin C (GPC). CD236 is an integral membrane protein which carries the Gerbich blood group antigens. Expression of CD236 is not restricted to erythroid cells, but is broadly distributed in both erythroid and non-erythroid tissues.
Mouse anti Human CD236 antibody, clone BRIC4 specifically reacts with an epitope located within residues 2-21 of CD236.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared from tissue culture supernatant
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Human erythrocytes.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P04921 Related reagents
Entrez Gene
GYPC Related reagents
GO Terms
GO:0005515 protein binding
GO:0005887 integral to plasma membrane
GO:0030863 cortical cytoskeleton
Regulatory
For research purposes only

Applications of CD236 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Haemagglutination
Immunohistology - Frozen
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Product Specific References

References for CD236 antibody

  1. Anstee, D.J .et al. (1984) Two individuals with elliptocytic red cells apparently lack three minor erythrocyte membrane sialoglycoproteins.
    Biochem J. 218 (2): 615-9.
  2. Dahr. W/ et al. (1989) High-frequency antigens of human erythrocyte membrane sialoglycoproteins. VI. Monoclonal antibodies reacting with the N-terminal domain of glycophorin C.
    Biol Chem Hoppe Seyler. 370 (8): 849-54.