CD236 antibody | BRIC4

Mouse anti Human CD236

Product Type
Monoclonal Antibody
Clone
BRIC4
Isotype
IgG1
Specificity
CD236

Product Code Applications Pack Size List Price Your Price Qty
MCA1986
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F H WB 0.2 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD236 antibody, clone BRIC4 recognizes the erythrocyte transmembrane glycoprotein CD236, also known as glycophorin C (GPC). CD236 is an integral membrane protein which carries the Gerbich blood group antigens. Expression of CD236 is not restricted to erythroid cells, but is broadly distributed in both erythroid and non-erythroid tissues.
Mouse anti Human CD236 antibody, clone BRIC4 specifically reacts with an epitope located within residues 2-21 of CD236.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
<0.1% sodium azide (NaN3)
≤100mM glycine
Immunogen
Human erythrocytes.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Haemagglutination
Immunohistology - Frozen
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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References for CD236 antibody

  1. Anstee, D.J .et al. (1984) Two individuals with elliptocytic red cells apparently lack three minor erythrocyte membrane sialoglycoproteins.
    Biochem J. 218 (2): 615-9.
  2. Dahr. W/ et al. (1989) High-frequency antigens of human erythrocyte membrane sialoglycoproteins. VI. Monoclonal antibodies reacting with the N-terminal domain of glycophorin C.
    Biol Chem Hoppe Seyler. 370 (8): 849-54.

Synonyms
Glycophorin C
RRID
AB_323277
UniProt
P04921
Entrez Gene
GYPC
GO Terms
GO:0005515 protein binding
GO:0005887 integral to plasma membrane
GO:0030863 cortical cytoskeleton
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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