CD227 antibody | C595 (NCRC48)

Mouse anti Human CD227

Product Type
Monoclonal Antibody
Clone
C595 (NCRC48)
Isotype
IgG3
Specificity
CD227

Product Code Applications Pack Size List Price Your Price Qty
MCA1742
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F IF P * WB 0.2 mg loader
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loader
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Mouse anti Human CD227 antibody, clone C595 (NCRC48) recognizes CD227, also known as mucin 1 which is a breast cancer associated mucin encoded by the Muc-1 gene. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.

Mouse anti Human CD227 antibody, clone C595 recognizes the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Urinary MUC-1 mucin.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of a mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/1000
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires heat mediated antigen retrieval prior to staining of paraffin embedded material. Citrate buffer pH6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Breast carcinoma

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References for CD227 antibody

  1. Price, M.R. et al. (1990) Immunological and structural features of the protein core of human polymorphic epithelial mucin.
    Mol Immunol. 27 (8): 795-802.
  2. Price, M.R. et al. (1990) C595--a monoclonal antibody against the protein core of human urinary epithelial mucin commonly expressed in breast carcinomas.
    Br J Cancer. 61 (5): 681-6.
  3. Price, M.R. et al. (1991) Purification of anti-epithelial mucin monoclonal antibodies by epitope affinity chromatography.
    J Immunol Methods. 139 (1): 83-90.
  4. Denton, G. et al. (1995) Primary sequence determination and molecular modelling of the variable region of an antiMUC1 mucin monoclonal antibody.
    Eur J Cancer. 31A (2): 214-21.
  5. Denton, G. et al. (1997) Production and characterization of a recombinant anti-MUC1 scFv reactive with human carcinomas.
    Br J Cancer. 76 (5): 614-21.
  6. Murray, A. et al (2001) Production and characterization of 188Re-C595 antibody for radioimmunotherapy of transitional cell bladder cancer.
    J Nucl Med. 42: 726-32.
  7. Hisatsune, A. et al. (2011) Anti-MUC1 antibody inhibits EGF receptor signaling in cancer cells.
    Biochem Biophys Res Commun. 405: 377-81.
  8. Ragupathi, G. et al. (2005) Antibodies against tumor cell glycolipids and proteins, but not mucins, mediate complement-dependent cytotoxicity.
    J Immunol. 174: 5706-12.
  9. View The Latest Product References
  10. Huang, D.M. et al. (2004) Modulation of anti-adhesion molecule MUC-1 is associated with arctiin-induced growth inhibition in PC-3 cells.
    Prostate. 2004 May 15;59(3): 260-7.

Synonyms
CA 15-3
MUCIN 1
RRID
AB_322211
UniProt
P15941
Entrez Gene
MUC1
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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