CD209 antibody | MR-1
Mouse anti Human CD209 antibody, clone MR-1 (MCA2318) used to evaluate DC-SIGN expression on cultured THP-1 cells by flow cytometry.
Image caption:
Role of DC-SIGN in the anti-DENV2 activity of bLF. The THP-1 cells were stimulated with recombinant IL-4, GM-CSF, and TNF-α according to a previous report [19], and dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) expression was subsequently measured by flow cytometry using mouse anti-human DC-SIGN antibodies and fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse immunoglobulin G (IgG) antibodies (A) The DC-SIGN-expressing and non-expessing THP-1 cells were treated with 200 μg/mL bLF, then infected with DENV-2 at MOI of 5, and analyzed by infectious center assay; (B) The infection rate equals 100 × (plaque number/cell number) (* p<0.05).
From: Chen JM, Fan YC, Lin JW, Chen YY, Hsu WL, Chiou SS.
Bovine Lactoferrin Inhibits Dengue Virus Infectivity by Interacting with Heparan Sulfate, Low-Density Lipoprotein Receptor, and DC-SIGN.
Int J Mol Sci. 2017 Sep 12;18(9). pii: E1957.
Mouse anti Human CD209:FITC
Mouse anti Human CD209:Low Endotoxin
Mouse anti Human CD209
Mouse anti Human CD209:RPE
- Product Type
- Monoclonal Antibody
- Clone
- MR-1
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA2318F | F | 0.1 mg |
|
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| MCA2318EL | F FN IF | 0.5 mg |
|
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| MCA2318T | F IF | 25 µg |
|
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| MCA2318 | F IF | 0.2 mg |
|
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| MCA2318PE | F | 100 Tests |
|
Mouse anti Human CD209 antibody, clone MR-1 recognizes human DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), a ~45 kDa C-type lectin that binds ICAM-3 also known as CD209. CD209 is primarily expressed on a population of immature dendritic cells in peripheral tissues and on immature myeloid monocyte-derived DC's in vitro.
CD209 is involved in dendritic cell (DC) migration and the initial interaction between DC's and naive T lymphocytes. CD209 also binds HIV-1 gp120 and plays a key role in the dissemination of HIV-1 by DC's.
Mouse anti Human CD209 antibody, clone MR-1 is reported to partially block the functional activity of DC-SIGN (Melero et al.).
CD209 is involved in dendritic cell (DC) migration and the initial interaction between DC's and naive T lymphocytes. CD209 also binds HIV-1 gp120 and plays a key role in the dissemination of HIV-1 by DC's.
Mouse anti Human CD209 antibody, clone MR-1 is reported to partially block the functional activity of DC-SIGN (Melero et al.).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Immature myeloid monocyte-derived dendritic cells (MDDCs).
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fuzed with cells of the Mouse SP2/0-Ag14 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
Applications of CD209 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | |||
| Functional Assays | |||
| Immunofluorescence | |||
| Flow Cytometry | 1/50 | 1/100 | |
| Immunofluorescence | |||
| Flow Cytometry | Neat | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
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| Mouse IgG1 Negative Control:Low Endotoxin | MCA928EL | C F | 0.5 mg |
|
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| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
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| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD209 antibody
-
Relloso, M. et al. (2002) DC-SIGN (CD209) expression is IL-4 dependent and is negatively regulated by IFN, TGF-beta, and anti-inflammatory agents.
J Immunol. 168 (6): 2634-43. -
Melero, I. et al. (2002) An anti-ICAM-2 (CD102) monoclonal antibody induces immune-mediated regressions of transplanted ICAM-2-negative colon carcinomas.
Cancer Res. 62 (11): 3167-74. -
Domínguez-Soto, A. et al. (2011) Dendritic Cell-Specific ICAM-3-Grabbing Nonintegrin Expression on M2-Polarized and Tumor-Associated Macrophages Is Macrophage-CSF Dependent and Enhanced by Tumor-Derived IL-6 and IL-10.
J Immunol. 186: 2192-200. -
Chang, S.K. et al. (2008) B lymphocyte stimulator regulates adaptive immune responses by directly promoting dendritic cell maturation.
J Immunol.180: 7394-403. -
Alvarez, C.P. et al (2002) C-type lectins DC-SIGN and L-SIGN mediate cellular entry by Ebola virus in cis and in trans.
J Virol. 76: 6841-4. -
Zhang, S.S. et al. (2008) Plasminogen activator Pla of Yersinia pestis utilizes murine DEC-205 (CD205) as a receptor to promote dissemination
J Biol Chem. 283: 31511-21. -
Relloso, M. et al. (2002) Dendritic cell (DC)-specific intercellular adhesion molecule 3 (ICAM-3)-grabbing nonintegrin (DC-SIGN, CD209), a C-type surface lectin in human DCs, is a receptor for Leishmania amastigotes.
J Biol Chem. 277:36766-9 -
Ciudad, M.T. et al. (2016) Analysis of the HLA-DR peptidome from human dendritic cells reveals high affinity repertoires and nonconventional pathways of peptide generation.
J Leukoc Biol. Jun 30. pii: jlb.6HI0216-069R. [Epub ahead of print] -
Chen, J.M. et al. (2017) Bovine Lactoferrin Inhibits Dengue Virus Infectivity by Interacting with Heparan Sulfate, Low-Density Lipoprotein Receptor, and DC-SIGN.
Int J Mol Sci. 18 (9)Sep 12 [Epub ahead of print].
