CD209 antibody | MR-1

Mouse anti Human CD209

Product Type
Monoclonal Antibody
Clone
MR-1
Isotype
IgG1
Specificity
CD209

Product Code Applications Pack Size List Price Your Price Qty
MCA2318T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 25 µg loader
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loader
MCA2318
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 0.2 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD209 antibody, clone MR-1 recognizes human DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), a ~45 kDa C-type lectin that binds ICAM-3 also known as CD209. CD209 is primarily expressed on a population of immature dendritic cells in peripheral tissues and on immature myeloid monocyte-derived DC's in vitro.
CD209 is involved in dendritic cell (DC) migration and the initial interaction between DC's and naive T lymphocytes. CD209 also binds HIV-1 gp120 and plays a key role in the dissemination of HIV-1 by DC's.
Mouse anti Human CD209 antibody, clone MR-1 is reported to partially block the functional activity of DC-SIGN (Melero et al.).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Immature myeloid monocyte-derived dendritic cells (MDDCs).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fuzed with cells of the Mouse SP2/0-Ag14 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunofluorescence
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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loader
Description Mouse IgG1 Negative Control

References for CD209 antibody

  1. Relloso, M. et al. (2002) DC-SIGN (CD209) expression is IL-4 dependent and is negatively regulated by IFN, TGF-beta, and anti-inflammatory agents.
    J Immunol. 168 (6): 2634-43.
  2. Melero, I. et al. (2002) An anti-ICAM-2 (CD102) monoclonal antibody induces immune-mediated regressions of transplanted ICAM-2-negative colon carcinomas.
    Cancer Res. 62 (11): 3167-74.
  3. Domínguez-Soto, A. et al. (2011) Dendritic Cell-Specific ICAM-3-Grabbing Nonintegrin Expression on M2-Polarized and Tumor-Associated Macrophages Is Macrophage-CSF Dependent and Enhanced by Tumor-Derived IL-6 and IL-10.
    J Immunol. 186: 2192-200.
  4. Chang, S.K. et al. (2008) B lymphocyte stimulator regulates adaptive immune responses by directly promoting dendritic cell maturation.
    J Immunol.180: 7394-403.
  5. Alvarez, C.P. et al (2002) C-type lectins DC-SIGN and L-SIGN mediate cellular entry by Ebola virus in cis and in trans.
    J Virol. 76: 6841-4.
  6. Zhang, S.S. et al. (2008) Plasminogen activator Pla of Yersinia pestis utilizes murine DEC-205 (CD205) as a receptor to promote dissemination
    J Biol Chem. 283: 31511-21.
  7. Relloso, M. et al. (2002) Dendritic cell (DC)-specific intercellular adhesion molecule 3 (ICAM-3)-grabbing nonintegrin (DC-SIGN, CD209), a C-type surface lectin in human DCs, is a receptor for Leishmania amastigotes.
    J Biol Chem. 277:36766-9
  8. Ciudad, M.T. et al. (2017) Analysis of the HLA-DR peptidome from human dendritic cells reveals high affinity repertoires and nonconventional pathways of peptide generation.
    J Leukoc Biol. 101 (1): 15-27.
  9. View The Latest Product References
  10. Chen, J.M. et al. (2017) Bovine Lactoferrin Inhibits Dengue Virus Infectivity by Interacting with Heparan Sulfate, Low-Density Lipoprotein Receptor, and DC-SIGN.
    Int J Mol Sci. 18 (9)Sep 12 [Epub ahead of print].

Flow Cytometry

Functional Assays

Synonyms
DC-SIGN
RRID
AB_566637
UniProt
Q9NNX6
Entrez Gene
CD209
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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