CD206 antibody | 15-2
Mouse anti CD206, clone 15-2 has been used extensively to monitor mannose receptor modulation in macrophages treated with a wide range of cytokines and growth factors (Chang et al. 2004) and to indicate CD206 as a marker for alternative activation of macrophages (Joerink et al. 2011).
- Target Species
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Purified human mannose receptor.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice where fused with cells of the SP2/0 Ag.14 mouse myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005537 mannose binding
- GO:0006898 receptor-mediated endocytosis
- For research purposes only
Applications of CD206 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Western Blotting 1|
- 1Clone 15-2 recognises a protein of approximately 175kDa under non-reducing conditions.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Mouse IgG1 Negative Control||MCA928||F||100 Tests|
Product Specific References
Antibody Characterization Reference
Noorman, F. et al. (1997) Monoclonal antibodies against the human mannose receptor as a specific marker in flow cytometry and immunohistochemistry for macrophages.
J Leukoc Biol. 61 (1): 63-72.
References for CD206 antibody
Barrett-Bergshoeff, M. et al. (1997) Monoclonal antibodies against the human mannose receptor that inhibit the binding of tissue-type plasminogen activator.
Thromb Haemost. 77: 718-24.
Koning, N. et al. (2009) Distribution of the immune inhibitory molecules CD200 and CD200R in the normal central nervous system and multiple sclerosis lesions suggests neuron-glia and glia-glia interactions.
J Neuropathol Exp Neurol. 68: 159-67.
Emara, M. et al. (2011) Recognition of the major cat allergen Fel d 1 through the cysteine-rich domain of the mannose receptor determines its allergenicity.
J Biol Chem. 286:13033-40.
Chang, S.K. et al. (2008) B lymphocyte stimulator regulates adaptive immune responses by directly promoting dendritic cell maturation.
J Immunol. 180: 7394-403.
MacKinnon, A.C. et al. (2008) Regulation of alternative macrophage activation by galectin-3.
J Immunol. 180: 2650-8.
Lai, W.K. et al. (2006) Expression of DC-SIGN and DC-SIGNR on human sinusoidal endothelium: a role for capturing hepatitis C virus particles.
Am J Pathol. 169: 200-8.
Ueno, N. et al. (2009) Differences in human macrophage receptor usage, lysosomal fusion kinetics and survival between logarithmic and metacyclic Leishmania infantum chagasi promastigotes.
Cell Microbiol. 11: 1827-41.
Kato, M. et al. (2000) Expression of multilectin receptors and comparative FITC-dextran uptake by human dendritic cells.
Int Immunol. 12:1511-9.
Chang, Y.C. et al. (2004) Modulation of macrophage differentiation and activation by decoy receptor 3.
J Leukoc Biol. 75: 486-94.
Yamamoto, H. et al. (2011) Sphingosylphosphorylcholine and lysosulfatide have inverse regulatory functions in monocytic cell differentiation into macrophages.
Arch Biochem Biophys. 506: 83-91.
He, L.Z. et al. (2007) Antigenic targeting of the human mannose receptor induces tumor immunity.
J Immunol. 178: 6259-67.
Sturge, J. et al. (2007) Mannose receptor regulation of macrophage cell migration.
J Leukoc Biol. 82: 585-93.
Torrelles, J.B. et al. (2006) Fine discrimination in the recognition of individual species of phosphatidyl-myo-inositol mannosides from Mycobacterium tuberculosis by C-type lectin pattern recognition receptors.
J Immunol. 177 (3): 1805-16.
Larsson, K. et al. (2015) COX/mPGES-1/PGE2 pathway depicts an inflammatory-dependent high-risk neuroblastoma subset.
Proc Natl Acad Sci U S A. 112 (26): 8070-5.
Stankevich, K.S. et al. (2015) Surface modification of biomaterials based on high-molecular polylactic acid and their effect on inflammatory reactions of primary human monocyte-derived macrophages: perspective for personalized therapy.
Mater Sci Eng C Mater Biol Appl. 51: 117-26.
Yamane, K. & Leung, K.P. (2016) Rabbit M1 and M2 macrophages can be induced by human recombinant GM-CSF and M-CSF.
FEBS Open Bio. 6 (9): 945-53.
Karna, S.L. et al. (2016) RNA-Seq Transcriptomic Responses of Full-Thickness Dermal Excision Wounds to Pseudomonas aeruginosa Acute and Biofilm Infection.
PLoS One. 11 (10): e0165312.
Özçelik H et al. (2015) Harnessing the multifunctionality in nature: a bioactive agent release system with self-antimicrobial and immunomodulatory properties.
Adv Healthc Mater. 4 (13): 2026-36.
Argueta-Donohué J et al. (2016) Differential phagocytosis of Leishmania mexicana promastigotes and amastigotes by monocyte-derived dendritic cells.
Microbiol Immunol. 60 (6): 369-81.
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