CD206 antibody | 15-2

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Mouse anti Human CD206

Product Type
Monoclonal Antibody
Clone
15-2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2155T C F IF WB* 25 µg
MCA2155 C F IF WB* 0.2 mg
Mouse anti Human CD206 monoclonal antibody, clone 15-2 recognizes human macrophage mannose receptor 1, also known as CD206 or C-type lectin domain family 13 member D-like. CD206 is a ~175 kDa single pass type I transmembrane glycoprotein belonging to the group of pattern recognition receptors (Paveley et al. 2011). CD206 has multiple carbohydrate recognition motifs and acts as a receptor for bacteria, fungi and other pathogens (Ezekowitz et al. 1990). CD206 is predominantly expressed in tissue macrophages and dendritic cells (Engering et al. 1997) and can also be found in a subpopulation of endothelial cells (Pack et al. 2007) and sperm cells (Cardona-Maya et al. 2006). CD206 can also be detected in a soluble form in human plasma and is elevated in patients with acute sepsis (Rødgaard-Hansen et al. 2013).

Mouse anti CD206, clone 15-2 has been used extensively to monitor mannose receptor modulation in macrophages treated with a wide range of cytokines and growth factors (Chang et al. 2004) and to indicate CD206 as a marker for alternative activation of macrophages (Joerink et al. 2011).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified human mannose receptor.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice where fused with cells of the SP2/0 Ag.14 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P22897 Related reagents
Entrez Gene
MRC1 Related reagents
GO Terms
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005537 mannose binding
GO:0006898 receptor-mediated endocytosis
Regulatory
For research purposes only

Applications of CD206 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Western Blotting 1
  1. 1Clone 15-2 recognises a protein of approximately 175kDa under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Flow Cytometry

Immunofluorescence

Western Blotting

Product Specific References

References for CD206 antibody

  1. Barrett-Bergshoeff, M. et al. (1997) Monoclonal antibodies against the human mannose receptor that inhibit the binding of tissue-type plasminogen activator.
    Thromb Haemost. 77: 718-24.
  2. Koning, N. et al. (2009) Distribution of the immune inhibitory molecules CD200 and CD200R in the normal central nervous system and multiple sclerosis lesions suggests neuron-glia and glia-glia interactions.
    J Neuropathol Exp Neurol. 68: 159-67.
  3. Emara, M. et al. (2011) Recognition of the major cat allergen Fel d 1 through the cysteine-rich domain of the mannose receptor determines its allergenicity.
    J Biol Chem. 286:13033-40.
  4. Chang, S.K. et al. (2008) B lymphocyte stimulator regulates adaptive immune responses by directly promoting dendritic cell maturation.
    J Immunol. 180: 7394-403.
  5. MacKinnon, A.C. et al. (2008) Regulation of alternative macrophage activation by galectin-3.
    J Immunol. 180: 2650-8.
  6. Lai, W.K. et al. (2006) Expression of DC-SIGN and DC-SIGNR on human sinusoidal endothelium: a role for capturing hepatitis C virus particles.
    Am J Pathol. 169: 200-8.
  7. Ueno, N. et al. (2009) Differences in human macrophage receptor usage, lysosomal fusion kinetics and survival between logarithmic and metacyclic Leishmania infantum chagasi promastigotes.
    Cell Microbiol. 11: 1827-41.
  8. Kato, M. et al. (2000) Expression of multilectin receptors and comparative FITC-dextran uptake by human dendritic cells.
    Int. Immunol. 12:1511-9.
  9. Chang, Y.C. et al. (2004) Modulation of macrophage differentiation and activation by decoy receptor 3.
    J Leukoc Biol. 75: 486-94.
  10. Yamamoto, H. et al. (2011) Sphingosylphosphorylcholine and lysosulfatide have inverse regulatory functions in monocytic cell differentiation into macrophages.
    Arch Biochem Biophys. 506: 83-91.
  11. He, L.Z. et al. (2007) Antigenic targeting of the human mannose receptor induces tumor immunity.
    J Immunol. 178: 6259-67.
  12. Sturge, J. et al. (2007) Mannose receptor regulation of macrophage cell migration.
    J Leukoc Biol. 82: 585-93.
  13. Torrelles, J.B. et al. (2006) Fine discrimination in the recognition of individual species of phosphatidyl-myo-inositol mannosides from Mycobacterium tuberculosis by C-type lectin pattern recognition receptors.
    J Immunol. 177 (3): 1805-16.
  14. Larsson, K. et al. (2015) COX/mPGES-1/PGE2 pathway depicts an inflammatory-dependent high-risk neuroblastoma subset.
    Proc Natl Acad Sci U S A. 112 (26): 8070-5.
  15. Stankevich, K.S. et al. (2015) Surface modification of biomaterials based on high-molecular polylactic acid and their effect on inflammatory reactions of primary human monocyte-derived macrophages: perspective for personalized therapy.
    Mater Sci Eng C Mater Biol Appl. 51: 117-26.
  16. Yamane, K. & Leung, K.P. (2016) Rabbit M1 and M2 macrophages can be induced by human recombinant GM-CSF and M-CSF.
    FEBS Open Bio. 6 (9): 945-53.
  17. Karna, S.L. et al. (2016) RNA-Seq Transcriptomic Responses of Full-Thickness Dermal Excision Wounds to Pseudomonas aeruginosa Acute and Biofilm Infection.
    PLoS One. 11 (10): e0165312.
  18. Özçelik H et al. (2015) Harnessing the multifunctionality in nature: a bioactive agent release system with self-antimicrobial and immunomodulatory properties.
    Adv Healthc Mater. 4 (13): 2026-36.
  19. Argueta-Donohué J et al. (2016) Differential phagocytosis of Leishmania mexicana promastigotes and amastigotes by monocyte-derived dendritic cells.
    Microbiol Immunol. 60 (6): 369-81.