CD184 antibody | 12G5
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|Mouse anti Human CD184 clone 12G5, recognizes CD184 also known as C-X-C chemokine receptor 4 (CXCR4) and fusion which is a G-protein-coupled chemokine receptor. It has an extracellular N-terminus, a 7-transmembrane structure with 7 helical regions connected by 6 membrane loops and a cytoplasmic C-terminus. It is expressed in a number of hematopoietic cells including hematopoietic stem cells, T cells, B cells, monocytes, macrophages, neutrophils and eosinophils (Teicher & Fricker 2010).
CD184’s ligand is C-X-C chemokine ligand 12 (CXCL12) which is also known as stromal cell-derived factor 1 (SDF-1). Upon binding of the ligand signaling events are initiated which can result in alterations in gene expression, actin polymerisation, cell skeleton rearrangement and cell migration (Domanska et al. 2013). Via these pathways CD184 plays a role in homing hematopoietic stem and progenitor cells in bone marrow and mobilizes the cells to the periphery during stress or injury (Pawig et al. 2015). As well as homeostatic processes, CD184 has also been linked with various pathologies, including systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis and tumorogenesis. Its expression in CD4+ T cells has also shown that it acts as a natural co-receptor for HIV type I by assisting with triggering membrane fusion of the virus to the target cell (Feng et al. 1996).
Clone 12G5 has been used in flow cytometry experiments to examine CD184 cell surface expression in cells modified to express Δ20 IFNITM2, an IFN-induced transmembrane protein which is known for its ability to inhibit the binding of several human viruses (Wu et al. 2017).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Primate
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- CP-MAC-infected Sup-T1 cells
- >95% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
This product should be stored undiluted.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin|
References for CD184 antibody
Wu, W.L. et al. (2017) Δ20 IFITM2 differentially restricts X4 and R5 HIV-1.
Proc Natl Acad Sci U S A. 114 (27): 7112-7117.
Pawig, L. et al. (2015) Diversity and Inter-Connections in the CXCR4 Chemokine Receptor/Ligand Family: Molecular Perspectives.
Front Immunol. 6: 429.
WalenkampA, M.E. et al. (2017) CXCR4 Ligands: The Next Big Hit?
J Nucl Med. 58 (Suppl 2): 77S-82S.
Hamm, H.E. (1998) The many faces of G protein signaling.
J Biol Chem. 273 (2): 669-72.
Teicher, B.A. & Fricker, S.P. (2010) CXCL12 (SDF-1)/CXCR4 pathway in cancer.
Clin Cancer Res. 16 (11): 2927-31.
Domanska, U.M. et al. (2013) A review on CXCR4/CXCL12 axis in oncology: no place to hide.
Eur J Cancer. 49 (1): 219-30.
- Entrez Gene
- GO Terms
- GO:0005886 plasma membrane
- GO:0000187 activation of MAPK activity
- GO:0001666 response to hypoxia
- GO:0003779 actin binding
- GO:0009615 response to virus
- GO:0006915 apoptosis
- GO:0016021 integral to membrane
- GO:0006954 inflammatory response
- GO:0007204 elevation of cytosolic calcium ion concentration
- View More GO Terms
- GO:0009986 cell surface
- GO:0015026 coreceptor activity
- GO:0016023 cytoplasmic membrane-bounded vesicle
- GO:0016493 C-C chemokine receptor activity
- GO:0016494 C-X-C chemokine receptor activity
- GO:0019059 initiation of viral infection
- GO:0030260 entry into host cell
- GO:0031252 cell leading edge
- GO:0031625 ubiquitin protein ligase binding
- GO:0032027 myosin light chain binding
- GO:0043130 ubiquitin binding
- GO:0050920 regulation of chemotaxis
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