CD184 antibody | 12G5

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Mouse anti Human CD184:APC

Mouse anti Human CD184

Mouse anti Human CD184:RPE

Product Type
Monoclonal Antibody
Clone
12G5
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA6126APC
Datasheet
F
MSDS
100 Tests/1ml loader

MCA6126
Datasheet
F FN IC P
MSDS
0.1 mg loader

MCA6126PE
Datasheet
F
MSDS
100 Tests/1ml loader

Mouse anti Human CD184 clone 12G5, recognizes CD184 also known as C-X-C chemokine receptor 4 (CXCR4) and fusion which is a G-protein-coupled chemokine receptor. It has an extracellular N-terminus, a 7-transmembrane structure with 7 helical regions connected by 6 membrane loops and a cytoplasmic C-terminus. It is expressed in a number of hematopoietic cells including hematopoietic stem cells, T cells, B cells, monocytes, macrophages, neutrophils and eosinophils (Teicher & Fricker 2010).

CD184’s ligand is C-X-C chemokine ligand 12 (CXCL12) which is also known as stromal cell-derived factor 1 (SDF-1). Upon binding of the ligand signaling events are initiated which can result in alterations in gene expression, actin polymerisation, cell skeleton rearrangement and cell migration (Domanska et al. 2013). Via these pathways CD184 plays a role in homing hematopoietic stem and progenitor cells in bone marrow and mobilizes the cells to the periphery during stress or injury (Pawig et al. 2015). As well as homeostatic processes, CD184 has also been linked with various pathologies, including systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis and tumorogenesis. Its expression in CD4+ T cells has also shown that it acts as a natural co-receptor for HIV type I by assisting with triggering membrane fusion of the virus to the target cell (Feng et al. 1996).

Clone 12G5 has been used in flow cytometry experiments to examine CD184 cell surface expression in cells modified to express Δ20 IFNITM2, an IFN-induced transmembrane protein which is known for its ability to inhibit the binding of several human viruses (Wu et al. 2017).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Primate
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Allophycocyanin (APC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Immunogen
CP-MAC-infected Sup-T1 cells
Purity
>95% by SDS PAGE
Purity
>95% by SDS PAGE
Purity
>95% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P61073
Entrez Gene
CXCR4
GO Terms
GO:0005886 plasma membrane
GO:0000187 activation of MAPK activity
GO:0001666 response to hypoxia
GO:0003779 actin binding
GO:0009615 response to virus
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0006954 inflammatory response
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0009986 cell surface
GO:0015026 coreceptor activity
GO:0016023 cytoplasmic membrane-bounded vesicle
GO:0016493 C-C chemokine receptor activity
GO:0016494 C-X-C chemokine receptor activity
GO:0019059 initiation of viral infection
GO:0030260 entry into host cell
GO:0031252 cell leading edge
GO:0031625 ubiquitin protein ligase binding
GO:0032027 myosin light chain binding
GO:0043130 ubiquitin binding
GO:0050920 regulation of chemotaxis
Regulatory
For research purposes only

Applications of CD184 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry
Functional Assays
Immunocytochemistry
Immunohistology - Paraffin
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F F 0.1 mg loader
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control:APC MCA929APC F 100 Tests loader
Mouse IgG2a Negative Control MCA929 F 100 Tests loader
Mouse IgG2a Negative Control:RPE MCA929PE F 100 Tests loader