CD184 antibody | 12G5

Mouse anti Human CD184

Product Type
Monoclonal Antibody
Clone
12G5
Isotype
IgG2a
Specificity
CD184

Product Code Applications Pack Size List Price Your Price Qty
MCA6126
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F FN IC P 0.1 mg loader
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loader
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Mouse anti Human CD184 clone 12G5, recognizes CD184 also known as C-X-C chemokine receptor 4 (CXCR4) and fusion which is a G-protein-coupled chemokine receptor. It has an extracellular N-terminus, a 7-transmembrane structure with 7 helical regions connected by 6 membrane loops and a cytoplasmic C-terminus. It is expressed in a number of hematopoietic cells including hematopoietic stem cells, T cells, B cells, monocytes, macrophages, neutrophils and eosinophils (Teicher & Fricker 2010).

CD184’s ligand is C-X-C chemokine ligand 12 (CXCL12) which is also known as stromal cell-derived factor 1 (SDF-1). Upon binding of the ligand signaling events are initiated which can result in alterations in gene expression, actin polymerisation, cell skeleton rearrangement and cell migration (Domanska et al. 2013). Via these pathways CD184 plays a role in homing hematopoietic stem and progenitor cells in bone marrow and mobilizes the cells to the periphery during stress or injury (Pawig et al. 2015). As well as homeostatic processes, CD184 has also been linked with various pathologies, including systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis and tumorogenesis. Its expression in CD4+ T cells has also shown that it acts as a natural co-receptor for HIV type I by assisting with triggering membrane fusion of the virus to the target cell (Feng et al. 1996).

Clone 12G5 has been used in flow cytometry experiments to examine CD184 cell surface expression in cells modified to express Δ20 IFNITM2, an IFN-induced transmembrane protein which is known for its ability to inhibit the binding of several human viruses (Wu et al. 2017).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Primate
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
CP-MAC-infected Sup-T1 cells
Purity
>95% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Functional Assays
Immunocytochemistry
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for CD184 antibody

  1. Wu, W.L. et al. (2017) Δ20 IFITM2 differentially restricts X4 and R5 HIV-1.
    Proc Natl Acad Sci U S A. 114 (27): 7112-7117.
  2. Pawig, L. et al. (2015) Diversity and Inter-Connections in the CXCR4 Chemokine Receptor/Ligand Family: Molecular Perspectives.
    Front Immunol. 6: 429.
  3. WalenkampA, M.E. et al. (2017) CXCR4 Ligands: The Next Big Hit?
    J Nucl Med. 58 (Suppl 2): 77S-82S.
  4. Hamm, H.E. (1998) The many faces of G protein signaling.
    J Biol Chem. 273 (2): 669-72.
  5. Teicher, B.A. & Fricker, S.P. (2010) CXCL12 (SDF-1)/CXCR4 pathway in cancer.
    Clin Cancer Res. 16 (11): 2927-31.
  6. Domanska, U.M. et al. (2013) A review on CXCR4/CXCL12 axis in oncology: no place to hide.
    Eur J Cancer. 49 (1): 219-30.

Synonyms
CXCR4
UniProt
P61073
Entrez Gene
CXCR4
GO Terms
GO:0005886 plasma membrane
GO:0000187 activation of MAPK activity
GO:0001666 response to hypoxia
GO:0003779 actin binding
GO:0009615 response to virus
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0006954 inflammatory response
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0009986 cell surface
GO:0015026 coreceptor activity
GO:0016023 cytoplasmic membrane-bounded vesicle
GO:0016493 C-C chemokine receptor activity
GO:0016494 C-X-C chemokine receptor activity
GO:0019059 initiation of viral infection
GO:0030260 entry into host cell
GO:0031252 cell leading edge
GO:0031625 ubiquitin protein ligase binding
GO:0032027 myosin light chain binding
GO:0043130 ubiquitin binding
GO:0050920 regulation of chemotaxis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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