CD18 antibody | MEM-148
Mouse anti Human CD18 (Activation Epitope):Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- MEM-148
- Isotype
- IgG1
- Specificity
- CD18
- Region
- (ACTIVATION EPITOPE)
Mouse anti Human CD18 (Activation Epitope) antibody, clone MEM-148 recognizes an epitope on the human CD18 molecule that is hidden in the CD11/CD18 heterodimer on resting cells. Clone MEM-148 binds very weakly to resting peripheral blood leukocytes and strongly to all leukocytes upon cellular activation. The epitope recognized by Mouse anti Human CD18 (Activation Epitope) antibody, clone MEM-148 is also exposed during dissociation of the CD11/CD18 by low pH. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None Present
- Immunogen
- Peripheral blood mononuclear cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/20 | 1/200 | |
Immunoprecipitation | |||
Western Blotting | Non-reducing conditions |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG1 Negative Control |
References for CD18 antibody
-
Drbal, K. et al. (2001) A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells.
Blood. 98 (5): 1561-6. -
Ehirchiou, D. et al. (2005) Dual function for a unique site within the beta2I domain of integrin alphaMbeta2.
J Biol Chem. 280: 8324-31. -
Solovjov, D.A. et al. (2005) Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2.
J Biol Chem. 280: 1336-45. -
Cairo, C.W. et al. (2006) Cytoskeletal regulation couples LFA-1 conformational changes to receptor lateral mobility and clustering.
Immunity. 25: 297-308. -
Shi, M. et al. (2007) A structural hypothesis for the transition between bent and extended conformations of the leukocyte beta2 integrins.
J Biol Chem. 282: 30198-206. -
Cheng, M. et al. (2007) Mutation of a conserved asparagine in the I-like domain promotes constitutively active integrins alphaLbeta2 and alphaIIbbeta3.
J Biol Chem. 282: 18225-32. -
Kudlová M. et al. (2007) Expression of an activated form of integrin beta2 chain CD18 in cardiac surgical operations.
Acta Medica (Hradec Kralove). 50: 187-93. -
Anogianaki, A. et al. (2007) Capsaicin an irritant anti-inflammatory compound.
J Biol Regul Homeost Agents. 21: 1-4.
View The Latest Product References
-
Tang, X.Y. et al. (2008) Intercellular adhesion molecule-3 binding of integrin alphaL beta2 requires both extension and opening of the integrin headpiece.
J Immunol. 180: 4793-804. -
Arthos, J. et al. (2008) HIV-1 envelope protein binds to and signals through integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.
Nat Immunol. 9: 301-9. -
Luissint, A.C. et al. (2008) JAM-L-mediated leukocyte adhesion to endothelial cells is regulated in cis by alpha4beta1 integrin activation.
J Cell Biol. 183 (6): 1159-73. -
Feng, C. et al. (2011) Endogenous PMN sialidase activity exposes activation epitope on CD11b/CD18 which enhances its binding interaction with ICAM-1.
J Leukoc Biol. 90: 313-21. -
Dilek, N. et al. (2013) Targeting CD28, CTLA-4 and PD-L1 costimulation differentially controls immune synapses and function of human regulatory and conventional T-cells.
PLoS One. 8 (12): e83139.
- Synonyms
- Integrin Beta 2 Chain
- RRID
- AB_323901
- UniProt
- P05107
- Entrez Gene
- ITGB2
- GO Terms
- GO:0001948 glycoprotein binding
- GO:0007596 blood coagulation
- GO:0006915 apoptosis
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0006954 inflammatory response
- GO:0007159 leukocyte cell-cell adhesion
- GO:0007160 cell-matrix adhesion
- GO:0007229 integrin-mediated signaling pathway
- View More GO Terms
- GO:0007267 cell-cell signaling
- GO:0007275 multicellular organismal development
- GO:0008360 regulation of cell shape
- GO:0019901 protein kinase binding
- GO:0030593 neutrophil chemotaxis
- GO:0050730 regulation of peptidyl-tyrosine phosphorylation
- GO:0050776 regulation of immune response
MCA2086XZ
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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