Mouse anti Human CD175 antibody, clone BRIC111 recognizes Tn antigen on glycophorin A and glycophorin B in human erythrocytes. Tn is a cryptantigen which was designated CD175 at the 7th Leucocyte Typing Workshop. Tn antigen is not expressed on normal haemopoietic cells but exposure of the Tn is associated with polyagglutination.
- Target Species
- Product Form
- Purified IgG - liquid
- Buffer Solution
- TRIS buffered glycine
- Preservative Stabilisers
- Human Tn red blood cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of a myeloma cell line
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD175 antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Breast carcinoma
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Secondary Antibodies Available
Product Specific References
King, M.J. et al. (1991) Immunochemical studies on the differential binding properties of two monoclonal antibodies reacting with Tn red cells.
Transfusion 31 (2): 142-9.
References for CD175 antibody
Deutscher, S.L. et al. (2010) Carbohydrate antigens in nipple aspirate fluid predict the presence of atypia and cancer in women requiring diagnostic breast biopsy.
BMC Cancer. 10: 519.
King, M.J. et al. (1994) Two different glycosyltransferase defects that result in GalNAc alpha-O-peptide (Tn) expression.
Glycobiology. 4 (3): 267-79.
Wua, A.M. et al. (2005) Further characterization of the binding properties of two monoclonal antibodies recognizing human Tn red blood cells.
J Biomed Sci. 12 (1): 153-66.
Cao, Y. (2008) Expression of CD175 (Tn), CD175s (sialosyl-Tn) and CD176 (Thomsen-Friedenreich antigen) on malignant human hematopoietic cells.
Int J Cancer. 123: 89-99.
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