CD175 antibody | BRIC111
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Mouse anti Human CD175 antibody, clone BRIC111 recognizes Tn antigen on glycophorin A and glycophorin B in human erythrocytes. Tn is a cryptantigen which was designated CD175 at the 7th Leucocyte Typing Workshop. Tn antigen is not expressed on normal haemopoietic cells but exposure of the Tn is associated with polyagglutination.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Buffer Solution
- TRIS buffered glycine
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Human Tn red blood cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of a myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of CD175 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Haemagglutination | |||
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Breast carcinoma
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
Source Reference
-
King, M.J. et al. (1991) Immunochemical studies on the differential binding properties of two monoclonal antibodies reacting with Tn red cells.
Transfusion 31 (2): 142-9.
References for CD175 antibody
-
Deutscher, S.L. et al. (2010) Carbohydrate antigens in nipple aspirate fluid predict the presence of atypia and cancer in women requiring diagnostic breast biopsy.
BMC Cancer. 10: 519. -
King, M.J. et al. (1994) Two different glycosyltransferase defects that result in GalNAc alpha-O-peptide (Tn) expression.
Glycobiology. 4 (3): 267-79. -
Wua, A.M. et al. (2005) Further characterization of the binding properties of two monoclonal antibodies recognizing human Tn red blood cells.
J Biomed Sci. 12 (1): 153-66.
Further Reading
-
Cao, Y.
(2008) Expression of CD175 (Tn), CD175s (sialosyl-Tn) and CD176 (Thomsen-Friedenreich antigen) on malignant human hematopoietic cells.
Int J Cancer. 123: 89-99.
