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CD175 antibody | BRIC111

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Mouse anti Human CD175

Product Type
Monoclonal Antibody
Clone
BRIC111
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1985
Datasheet
C E H
SDS
0.2 mg loader

Mouse anti Human CD175 antibody, clone BRIC111 recognizes Tn antigen on glycophorin A and glycophorin B in human erythrocytes. Tn is a cryptantigen which was designated CD175 at the 7th Leucocyte Typing Workshop. Tn antigen is not expressed on normal haemopoietic cells but exposure of the Tn is associated with polyagglutination.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
TRIS buffered glycine
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Human Tn red blood cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of a myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD175 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Haemagglutination
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Breast carcinoma
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

Source Reference

  1. King, M.J. et al. (1991) Immunochemical studies on the differential binding properties of two monoclonal antibodies reacting with Tn red cells.
    Transfusion 31 (2): 142-9.

References for CD175 antibody

  1. Deutscher, S.L. et al. (2010) Carbohydrate antigens in nipple aspirate fluid predict the presence of atypia and cancer in women requiring diagnostic breast biopsy.
    BMC Cancer. 10: 519.
  2. King, M.J. et al. (1994) Two different glycosyltransferase defects that result in GalNAc alpha-O-peptide (Tn) expression.
    Glycobiology. 4 (3): 267-79.
  3. Wua, A.M. et al. (2005) Further characterization of the binding properties of two monoclonal antibodies recognizing human Tn red blood cells.
    J Biomed Sci. 12 (1): 153-66.

Further Reading

  1. Cao, Y. (2008) Expression of CD175 (Tn), CD175s (sialosyl-Tn) and CD176 (Thomsen-Friedenreich antigen) on malignant human hematopoietic cells.
    Int J Cancer. 123: 89-99.

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