CD173 antibody | BRIC231
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Mouse anti Human CD173
- Product Type
- Monoclonal Antibody
- Clone
- BRIC231
- Isotype
- IgG1
- Specificity
- CD173
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| Mouse anti Human CD173 antibody, clone BRIC231 recognizes human type 2 H blood group antigen, also known as CD173. Active H substances in man, are expressed by many cells and tissues and also by erythrocytes. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- TRIS buffered glycine
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Human erythroleukemic cell line (HEL) established from a 30 year old patient with relapsed erythroleukemia following treatment for Hodgkin lymphoma.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
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| Haemagglutination | |
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| Immunofluorescence | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD173 antibody
-
Clausen, H. and Hakomori, S. (1989) ABH and related histo-blood group antigens; immunochemical differences in carrier isotypes and their distribution.
Vox Sang 56(1): 1-20. -
Siegel, G. et al. (2013) Phenotype, donor age and gender affect function of human bone marrow-derived mesenchymal stromal cells.
BMC Med. 11: 146. -
Hotta, H. et al. (2013) Lewis y antigen is expressed in oral squamous cell carcinoma cell lines and tissues, but disappears in the invasive regions leading to the enhanced malignant properties irrespective of sialyl-Lewis x.
Glycoconj J. 30: 585-97. -
Guix, S. et al. (2007) Norwalk virus RNA is infectious in mammalian cells.
J Virol. 81: 12238-48. -
Sharpe, C. et al. (2014) Mixed field reactions in ABO and Rh typing chimerism likely resulting from twin haematopoiesis.
Blood Transfus. 12: 608-10. -
Cheetham, S. et al. (2007) Binding patterns of human norovirus-like particles to buccal and intestinal tissues of gnotobiotic pigs in relation to A/H histo-blood group antigen expression.
J Virol. 81: 3535-44. -
Hutson, A.M. et al. (2003) Norwalk virus-like particle hemagglutination by binding to h histo-blood group antigens.
J Virol. 77: 405-15. -
Matsumoto, S. et al. (2015) A Cytotoxic Antibody Recognizing Lacto-N-fucopentaose I (LNFP I) on Human Induced Pluripotent Stem (hiPS) Cells.
J Biol Chem. 290 (33): 20071-85.
View The Latest Product References
-
Schäfer R, et al.. (2020) Modulating endothelial adhesion and migration impacts stem cell therapies efficacy.
EBioMedicine. 60:102987. -
Lin, R-J. et al.. (2019) B3GALT5 Knockout Alters Glycosphingolipid Profile and Facilitates Transition to Human Naïve Pluripotency (December 27, 2019).
Stem Cell Dec 27 [Epub ahead of print - Reviewed]
- Synonyms
- Blood Group H Type 2
- RRID
- AB_323275
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