CD173 antibody | BRIC231

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Mouse anti Human CD173

Product Type: Monoclonal Antibody
Clone: BRIC231
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1984	F H IF			0.2 mg

Mouse anti Human CD173 antibody, clone BRIC231 recognizes human type 2 H blood group antigen, also known as CD173. Active H substances in man, are expressed by many cells and tissues and also by erythrocytes.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Pig

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Buffer Solution

TRIS buffered glycine

Preservative Stabilisers

0.09%

Sodium Azide

Immunogen

Human erythroleukemic cell line (HEL) established from a 30 year old patient with relapsed erythroleukemia following treatment for Hodgkin lymphoma.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

Regulatory: For research purposes only

Applications of CD173 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry
Haemagglutination
Immunofluorescence

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Application Based External Images

Flow Cytometry

Haemagglutination

Immunofluorescence

Product Specific References

References for CD173 antibody

Clausen, H. and Hakomori, S. (1989) ABH and related histo-blood group antigens; immunochemical differences in carrier isotypes and their distribution.
Vox Sang 56(1): 1-20.
Siegel, G. et al. (2013) Phenotype, donor age and gender affect function of human bone marrow-derived mesenchymal stromal cells.
BMC Med. 11: 146.
Hotta, H. et al. (2013) Lewis y antigen is expressed in oral squamous cell carcinoma cell lines and tissues, but disappears in the invasive regions leading to the enhanced malignant properties irrespective of sialyl-Lewis x.
Glycoconj J. 30: 585-97.
Guix, S. et al. (2007) Norwalk virus RNA is infectious in mammalian cells.
J Virol. 81: 12238-48.
Sharpe, C. et al. (2014) Mixed field reactions in ABO and Rh typing chimerism likely resulting from twin haematopoiesis.
Blood Transfus. 12: 608-10.
Cheetham, S. et al. (2007) Binding patterns of human norovirus-like particles to buccal and intestinal tissues of gnotobiotic pigs in relation to A/H histo-blood group antigen expression.
J Virol. 81: 3535-44.
Hutson, A.M. et al. (2003) Norwalk virus-like particle hemagglutination by binding to h histo-blood group antigens.
J Virol. 77: 405-15.
Matsumoto, S. et al. (2015) A Cytotoxic Antibody Recognizing Lacto-N-fucopentaose I (LNFP I) on Human Induced Pluripotent Stem (hiPS) Cells.
J Biol Chem. 290 (33): 20071-85.

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