CD173 antibody | BRIC231
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Mouse anti Human CD173 antibody, clone BRIC231 recognizes human type 2 H blood group antigen, also known as CD173. Active H substances in man, are expressed by many cells and tissues and also by erythrocytes.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- TRIS buffered glycine
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Human erythroleukemic cell line (HEL) established from a 30 year old patient with relapsed erythroleukemia following treatment for Hodgkin lymphoma.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of CD173 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Haemagglutination | |||
| Immunofluorescence |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F |
Product Specific References
References for CD173 antibody
-
Clausen, H. and Hakomori, S. (1989) ABH and related histo-blood group antigens; immunochemical differences in carrier isotypes and their distribution.
Vox Sang 56(1): 1-20. -
Siegel, G. et al. (2013) Phenotype, donor age and gender affect function of human bone marrow-derived mesenchymal stromal cells.
BMC Med. 11: 146. -
Hotta, H. et al. (2013) Lewis y antigen is expressed in oral squamous cell carcinoma cell lines and tissues, but disappears in the invasive regions leading to the enhanced malignant properties irrespective of sialyl-Lewis x.
Glycoconj J. 30: 585-97. -
Guix, S. et al. (2007) Norwalk virus RNA is infectious in mammalian cells.
J Virol. 81: 12238-48. -
Sharpe, C. et al. (2014) Mixed field reactions in ABO and Rh typing chimerism likely resulting from twin haematopoiesis.
Blood Transfus. 12: 608-10. -
Cheetham, S. et al. (2007) Binding patterns of human norovirus-like particles to buccal and intestinal tissues of gnotobiotic pigs in relation to A/H histo-blood group antigen expression.
J Virol. 81: 3535-44. -
Hutson, A.M. et al. (2003) Norwalk virus-like particle hemagglutination by binding to h histo-blood group antigens.
J Virol. 77: 405-15. -
Matsumoto, S. et al. (2015) A Cytotoxic Antibody Recognizing Lacto-N-fucopentaose I (LNFP I) on Human Induced Pluripotent Stem (hiPS) Cells.
J Biol Chem. 290 (33): 20071-85.
