Mouse anti Human CD173 antibody, clone BRIC231 recognizes human type 2 H blood group antigen, also known as CD173. Active H substances in man, are expressed by many cells and tissues and also by erythrocytes.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- TRIS buffered glycine
- Preservative Stabilisers
- Human erythroleukemic cell line (HEL) established from a 30 year old patient with relapsed erythroleukemia following treatment for Hodgkin lymphoma.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD173 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for CD173 antibody
Clausen, H. and Hakomori, S. (1989) ABH and related histo-blood group antigens; immunochemical differences in carrier isotypes and their distribution.
Vox Sang 56(1): 1-20.
Siegel, G. et al. (2013) Phenotype, donor age and gender affect function of human bone marrow-derived mesenchymal stromal cells.
BMC Med. 11: 146.
Hotta, H. et al. (2013) Lewis y antigen is expressed in oral squamous cell carcinoma cell lines and tissues, but disappears in the invasive regions leading to the enhanced malignant properties irrespective of sialyl-Lewis x.
Glycoconj J. 30: 585-97.
Guix, S. et al. (2007) Norwalk virus RNA is infectious in mammalian cells.
J Virol. 81: 12238-48.
Sharpe, C. et al. (2014) Mixed field reactions in ABO and Rh typing chimerism likely resulting from twin haematopoiesis.
Blood Transfus. 12: 608-10.
Cheetham, S. et al. (2007) Binding patterns of human norovirus-like particles to buccal and intestinal tissues of gnotobiotic pigs in relation to A/H histo-blood group antigen expression.
J Virol. 81: 3535-44.
Hutson, A.M. et al. (2003) Norwalk virus-like particle hemagglutination by binding to h histo-blood group antigens.
J Virol. 77: 405-15.
Matsumoto, S. et al. (2015) A Cytotoxic Antibody Recognizing Lacto-N-fucopentaose I (LNFP I) on Human Induced Pluripotent Stem (hiPS) Cells.
J Biol Chem. 290 (33): 20071-85.
Schäfer R, et al.. (2020) Modulating endothelial adhesion and migration impacts stem cell therapies efficacy.
Lin, R-J. et al.. (2019) B3GALT5 Knockout Alters Glycosphingolipid Profile and Facilitates Transition to Human Naïve Pluripotency (December 27, 2019).
Stem Cell Dec 27 [Epub ahead of print - Reviewed]
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra