CD169 antibody | 7-239

Mouse anti Human CD169

Product Type
Monoclonal Antibody
Clone
7-239
Isotype
IgG1
Specificity
CD169

Product Code Applications Pack Size List Price Your Price Qty
MCA6125
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F FN IP WB 0.1 mg loader
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loader
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Mouse anti Human CD169 clone 7-239, recognizes CD169 also known as Siglec-1 or Sialoadhesin, is a member of the Siglec family of proteins. It is expressed by subpopulations of macrophages and dendritic cells. Some subpopulations of macrophages express CD169 at a low level, but this expression can be upregulated upon induction by IFN-α (O’Neill et al. 2013). CD169+ cells are largely found in the lymph nodes, spleen, but are also present in smaller amounts in intestinal tracts, liver and bone marrow (Hartnell et al. 2001). The most characterized functions of CD169 are its roles in cell-cell interactions and phagocytosis of sialylated pathogens.

CD169 has an approximate molecular weight of 185 kDa and recognizes sialic acid-containing sugar chains. Structurally, it contains an extracellular domain containing 17 immunoglobulin-like domains and one v-set domain via which it binds its’ ligands. It also contains 16 C2-set domains which extend the binding site away from the surface of the cell. This extension helps bind granulocytes, B cells, erythrocytes and a subset of CD8 T cells (Eakin et al. 2016).

Increased expression of CD169 has been found to be associated with various conditions, including atherosclerosis, type I diabetes, chronic rejection and systemic sclerosis (Bornhöfft et al. 2018).

Mouse anti Human CD169 clone 7-239 has been used in flow cytometry experiments to measure cell surface expression of CD169 upon cell stimulation with IFN-α (OhAinle et al. 2018).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Human rhinovirus 14-infected monocyte-derived dendritic cells
Purity
>95% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for CD169 antibody

  1. Hammonds, J.E. et al. (2017) Siglec-1 initiates formation of the virus-containing compartment and enhances macrophage-to-T cell transmission of HIV-1.
    PLoS Pathog. 13 (1): e1006181.
  2. Izquierdo-useros, N. et al. (2012) Siglec-1 is a novel dendritic cell receptor that mediates HIV-1 trans-infection through recognition of viral membrane gangliosides.
    PLoS Biol. 10 (12): e1001448.
  3. Pino, M. et al. (2015) HIV-1 immune activation induces Siglec-1 expression and enhances viral trans-infection in blood and tissue myeloid cells.
    Retrovirology. 12: 37.
  4. Martinez-picado, J. et al. (2016) Identification of Siglec-1 null individuals infected with HIV-1.
    Nat Commun. 7: 12412.
  5. Perez-Zsolt, D. et al. (2019) Anti-Siglec-1 antibodies block Ebola viral uptake and decrease cytoplasmic viral entry.
    Nat Microbiol. 4 (9): 1558-1570.
  6. Rose, T. et al. (2017) Are interferon-related biomarkers advantageous for monitoring disease activity in systemic lupus erythematosus? A longitudinal benchmark study.
    Rheumatology (Oxford). 56 (9): 1618-26.
  7. Sharma, V. et al. (2021) Cerebrospinal fluid CD4+ T cell infection in humans and macaques during acute HIV-1 and SHIV infection.
    PLoS Pathog. 17 (12): e1010105.

Further Reading

  1. OhAinle, M. et al. (2018) A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV.
    Elife. 7Dec 06 [Epub ahead of print].
  2. Hartnell, A. et al. (2001) Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations.
    Blood. 97 (1): 288-96.
  3. Eakin, A.J. et al. (2016) Siglec-1 and -2 as potential biomarkers in autoimmune disease.
    Proteomics Clin Appl. 10 (6): 635-44.
  4. Bornhöfft, K.F. et al. (2018) Siglecs: A journey through the evolution of sialic acid-binding immunoglobulin-type lectins.
    Dev Comp Immunol. 86: 219-231.

Synonyms
Siglec-1
UniProt
Q9BZZ2
Entrez Gene
SIGLEC1
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0005529 sugar binding
GO:0006897 endocytosis
GO:0006954 inflammatory response
GO:0007160 cell-matrix adhesion
GO:0016337 cell-cell adhesion
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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