CD169 antibody | 7-239
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Mouse anti Human CD169
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD169 clone 7-239, recognizes CD169 also known as Siglec-1 or Sialoadhesin, is a member of the Siglec family of proteins. It is expressed by subpopulations of macrophages and dendritic cells. Some subpopulations of macrophages express CD169 at a low level, but this expression can be upregulated upon induction by IFN-α (O’Neill et al. 2013). CD169+ cells are largely found in the lymph nodes, spleen, but are also present in smaller amounts in intestinal tracts, liver and bone marrow (Hartnell et al. 2001). The most characterized functions of CD169 are its roles in cell-cell interactions and phagocytosis of sialylated pathogens.
CD169 has an approximate molecular weight of 185 kDa and recognizes sialic acid-containing sugar chains. Structurally, it contains an extracellular domain containing 17 immunoglobulin-like domains and one v-set domain via which it binds its’ ligands. It also contains 16 C2-set domains which extend the binding site away from the surface of the cell. This extension helps bind granulocytes, B cells, erythrocytes and a subset of CD8 T cells (Eakin et al. 2016).
Increased expression of CD169 has been found to be associated with various conditions, including atherosclerosis, type I diabetes, chronic rejection and systemic sclerosis (Bornhöfft et al. 2018).
Mouse anti Human CD169 clone 7-239 has been used in flow cytometry experiments to measure cell surface expression of CD169 upon cell stimulation with IFN-α (OhAinle et al. 2018).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Human rhinovirus 14-infected monocyte-derived dendritic cells
- >95% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
This product should be stored undiluted.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
References for CD169 antibody
Hammonds, J.E. et al. (2017) Siglec-1 initiates formation of the virus-containing compartment and enhances macrophage-to-T cell transmission of HIV-1.
PLoS Pathog. 13 (1): e1006181.
Izquierdo-useros, N. et al. (2012) Siglec-1 is a novel dendritic cell receptor that mediates HIV-1 trans-infection through recognition of viral membrane gangliosides.
PLoS Biol. 10 (12): e1001448.
Pino, M. et al. (2015) HIV-1 immune activation induces Siglec-1 expression and enhances viral trans-infection in blood and tissue myeloid cells.
Retrovirology. 12: 37.
Martinez-picado, J. et al. (2016) Identification of Siglec-1 null individuals infected with HIV-1.
Nat Commun. 7: 12412.
Perez-Zsolt, D. et al. (2019) Anti-Siglec-1 antibodies block Ebola viral uptake and decrease cytoplasmic viral entry.
Nat Microbiol. 4 (9): 1558-1570.
Rose, T. et al. (2017) Are interferon-related biomarkers advantageous for monitoring disease activity in systemic lupus erythematosus? A longitudinal benchmark study.
Rheumatology (Oxford). 56 (9): 1618-26.
Sharma, V. et al. (2021) Cerebrospinal fluid CD4+ T cell infection in humans and macaques during acute HIV-1 and SHIV infection.
PLoS Pathog. 17 (12): e1010105.
Hartnell, A. et al. (2001) Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations.
Blood. 97 (1): 288-96.
Eakin, A.J. et al. (2016) Siglec-1 and -2 as potential biomarkers in autoimmune disease.
Proteomics Clin Appl. 10 (6): 635-44.
Bornhöfft, K.F. et al. (2018) Siglecs: A journey through the evolution of sialic acid-binding immunoglobulin-type lectins.
Dev Comp Immunol. 86: 219-231.
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