CD166 antibody | 3A6

Product Code Applications Pack Size List Price Your Price Qty
MCA1926
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F IP 0.2 mg loader
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loader
MCA1926GA
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SDS Safety Datasheet SDS
C E F IP 0.1 mg loader
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loader
MCA1926T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F IP 25 µg loader
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loader
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Mouse anti Human CD166 antibody, clone 3A6 recognizes the 100 kDa adhesion molecule CD166, also known as ALCAM. CD166 is a member of the Ig superfamily and is expressed on activated T-cells, B cells and other cells including thymic epithelial cells, fibroblasts, keratinocytes and neurons. CD6 has been identified as a receptor for ALCAM (Skonier et al. 1996).

Mouse anti Human CD166 antibody, clone 3A6 is reported to cross-react with CD166 on ovine tissues and provides a useful tool for the identification and characterization of ovine mesenchymal stem cells in conjunction with CD44 which is expressed by this cell lineage and the hematopoietic cell marker CD45 which is not expressed on mesenchymal stem cells (Sanjurjo-Rodríguez et al. 2017).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
MCA1926, MCA1926GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA1926T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human thymic epithelial cells.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the P3X63 Ag8 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/10 1/50
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

References for CD166 antibody

  1. Yeh, S.P. et al. (2005) Mesenchymal stem cells can be easily isolated from bone marrow of patients with various haematological malignancies but the surface antigens expression may be changed after prolonged ex vivo culture.
    Leukemia. 19: 1505-7.
  2. Patel, D. D. et al. (1997) CD166 Workshop: Tissue distribution and functional analysis of antibodies reactive for CD166, a ligand for CD6.
    In Leukocyte Typing IV. Kishimoto, T. et al. eds Garland publishing Inc. New York p. 461-4.
  3. Tondreau, T. et al. (2008) Gene expression pattern of functional neuronal cells derived from human bone marrow mesenchymal stromal cells.
    BMC Genomics. 9:166.
  4. Wang, D. et al. (2004) Proteomic profiling of bone marrow mesenchymal stem cells upon transforming growth factor beta1 stimulation.
    J Biol Chem. 279 (42): 43725-34.
  5. Green, L.R. et al. (2011) Cooperative role for tetraspanins in adhesin-mediated attachment of bacterial species to human epithelial cells.
    Infect Immun. 79 (6): 2241-9.
  6. Agha-Hosseini, F. et al. (2010) In vitro isolation of stem cells derived from human dental pulp.
    Clin Transplant. 24: E23-8.
  7. Bhattacharya, S. et al. (2010) Toponome imaging system: in situ protein network mapping in normal and cancerous colon from the same patient reveals more than five-thousand cancer specific protein clusters and their subcellular annotation by using a three symbol code.
    J Proteome Res. 9: 6112-25.
  8. Ali, H. et al. (2015) Multi-Lineage Differentiation of Human Umbilical Cord Wharton's Jelly Mesenchymal Stromal Cells Mediates Changes in the Expression Profile of Stemness Markers.
    PLoS One. 10 (4): e0122465.
  9. View The Latest Product References
  10. Holmannova D et al. (2016) Effects of conventional CPB and mini-CPB on neutrophils CD162, CD166 and CD195 expression.
    Perfusion. Sep 13. pii: 0267659116669586. [Epub ahead of print]
  11. Prins, H.J. et al. (2016) Bone Regeneration Using the Freshly Isolated Autologous Stromal Vascular Fraction of Adipose Tissue in Combination With Calcium Phosphate Ceramics.
    Stem Cells Transl Med. 5 (10): 1362-1374.
  12. Srouji, S. et al. (2009) The Schneiderian membrane contains osteoprogenitor cells: in vivo and in vitro study.
    Calcif Tissue Int. 84 (2): 138-45.
  13. Katsube, Y. et al. (2010) Restoration of cellular function of mesenchymal stem cells from a hypophosphatasia patient.
    Gene Ther. 17 (4): 494-502.
  14. Fridriksdottir, A.J. et al. (2015) Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture.
    Nat Commun. 6: 8786.
  15. Brune, J.C. et al. (2011) Mesenchymal stromal cells from primary osteosarcoma are non-malignant and strikingly similar to their bone marrow counterparts.
    Int J Cancer. 129 (2): 319-30.
  16. Chen, F. et al. (2018) Bone morphogenetic protein 7-transduced human dermal-derived fibroblast cells differentiate into osteoblasts and form bone in vivo.
    Connect Tissue Res. 59 (3): 223-232.
  17. Juan, C.H. et al. (2020) In Vitro Differentiation of Human Placenta-Derived Multipotent Cells into Schwann-Like Cells.
    Biomolecules. 10 (12) Dec 10 [Epub ahead of print].

Flow Cytometry

Immunofluorescence

Synonyms
ALCAM
RRID
AB_323338
UniProt
Q13740
Entrez Gene
ALCAM
GO Terms
GO:0005102 receptor binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0007165 signal transduction
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