CD166 antibody | 3A6






Mouse anti Human CD166:Alexa Fluor® 488
Mouse anti Human CD166:Alexa Fluor® 647
Mouse anti Human CD166:FITC
Mouse anti Human CD166
Mouse anti Human CD166:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 3A6
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1926A488T | F | 25 Tests/0.25ml |
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MCA1926A488 | F | 100 Tests/1ml |
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MCA1926A647T | F | 25 Tests/0.25ml |
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MCA1926A647 | F | 100 Tests/1ml |
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MCA1926FT | F | 25 µg |
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MCA1926F | F | 0.1 mg |
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MCA1926T | C E F IP | 25 µg |
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MCA1926GA | C E F IP | 0.1 mg |
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MCA1926 | C E F IP | 0.2 mg |
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MCA1926PE | F | 100 Tests |
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Mouse anti Human CD166 antibody, clone 3A6 is reported to cross-react with CD166 on ovine tissues and provides a useful tool for the identification and characterization of ovine mesenchymal stem cells in conjunction with CD44 which is expressed by this cell lineage and the hematopoietic cell marker CD45 which is not expressed on mesenchymal stem cells (Sanjurjo-Rodríguez et al. 2017).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Immunogen
- Human thymic epithelial cells.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the P3X63 Ag8 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of reconstitution
More Information
- UniProt
- Q13740 Related reagents
- Entrez Gene
- ALCAM Related reagents
- GO Terms
- GO:0005102 receptor binding
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0007165 signal transduction
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD166 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | ||
Flow Cytometry | Neat | 1/5 | |
Flow Cytometry | Neat | ||
ELISA | |||
Flow Cytometry | 1/10 | 1/50 | |
Immunohistology - Frozen | |||
Immunoprecipitation | |||
Flow Cytometry |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | F | 100 Tests/1ml |
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Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
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Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD166 antibody
-
Yeh, S.P. et al. (2005) Mesenchymal stem cells can be easily isolated from bone marrow of patients with various haematological malignancies but the surface antigens expression may be changed after prolonged ex vivo culture.
Leukemia. 19: 1505-7. -
Patel, D. D. et al. (1997) CD166 Workshop: Tissue distribution and functional analysis of antibodies reactive for CD166, a ligand for CD6.
In Leukocyte Typing IV. Kishimoto, T. et al. eds Garland publishing Inc. New York p. 461-4. -
Tondreau, T. et al. (2008) Gene expression pattern of functional neuronal cells derived from human bone marrow mesenchymal stromal cells.
BMC Genomics. 9:166. -
Wang, D. et al. (2004) Proteomic profiling of bone marrow mesenchymal stem cells upon transforming growth factor beta1 stimulation.
J Biol Chem. 279 (42): 43725-34. -
Green, L.R. et al. (2011) Cooperative role for tetraspanins in adhesin-mediated attachment of bacterial species to human epithelial cells.
Infect Immun. 79 (6): 2241-9. -
Agha-Hosseini, F. et al. (2010) In vitro isolation of stem cells derived from human dental pulp.
Clin Transplant. 24: E23-8. -
Bhattacharya, S. et al. (2010) Toponome imaging system: in situ protein network mapping in normal and cancerous colon from the same patient reveals more than five-thousand cancer specific protein clusters and their subcellular annotation by using a three symbol code.
J Proteome Res. 9: 6112-25. -
Ali, H. et al. (2015) Multi-Lineage Differentiation of Human Umbilical Cord Wharton's Jelly Mesenchymal Stromal Cells Mediates Changes in the Expression Profile of Stemness Markers.
PLoS One. 10 (4): e0122465. -
Holmannova D et al. (2016) Effects of conventional CPB and mini-CPB on neutrophils CD162, CD166 and CD195 expression.
Perfusion. Sep 13. pii: 0267659116669586. [Epub ahead of print] -
Prins, H.J. et al. (2016) Bone Regeneration Using the Freshly Isolated Autologous Stromal Vascular Fraction of Adipose Tissue in Combination With Calcium Phosphate Ceramics.
Stem Cells Transl Med. 5 (10): 1362-1374. -
Srouji, S. et al. (2009) The Schneiderian membrane contains osteoprogenitor cells: in vivo and in vitro study.
Calcif Tissue Int. 84 (2): 138-45. -
Katsube, Y. et al. (2010) Restoration of cellular function of mesenchymal stem cells from a hypophosphatasia patient.
Gene Ther. 17 (4): 494-502. -
Fridriksdottir, A.J. et al. (2015) Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture.
Nat Commun. 6: 8786. -
Brune, J.C. et al. (2011) Mesenchymal stromal cells from primary osteosarcoma are non-malignant and strikingly similar to their bone marrow counterparts.
Int J Cancer. 129 (2): 319-30. -
Chen, F. et al. (2018) Bone morphogenetic protein 7-transduced human dermal-derived fibroblast cells differentiate into osteoblasts and form bone in vivo.
Connect Tissue Res. 59 (3): 223-232.