CD158d antibody | mAb#33

Mouse anti Human CD158d

Product Type
Monoclonal Antibody
Clone
mAb#33
Isotype
IgG1
Specificity
CD158d

Product Code Applications Pack Size List Price Your Price Qty
MCA6122
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F FN IC IP WB 0.1 mg loader
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loader
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Mouse anti Human CD158d, clone mAb#33, recognizes the extracellular portion of CD158d also known as Killer Cell Immunoglobulin-like Receptor, 2 domains, long cytoplasmic tail, 4 (KIR2DL4). CD158d is a 45 kDa natural killer (NK) cell marker, although cell surface expression and function depends on the genotype of particular individuals. As some individual have no detectable CD158d on the surface of resting NK cells. In some individuals surface levels of CD158d can increase upon stimulation by IL-2 (Kikuchi-Maki et a. 2003). The killer cell Ig-like receptor protein regulates the responsiveness of NK cells upon their contact with tumor cells, virus infected cells, cytokines and chemokines. CD158d is constitutively expressed by NK cells and recognizes major histocompatibility complex (MHC) class I molecules on target cells. The non-classical MHC class I molecule HLA-G is the only known ligand of CD158d (Rahagioakab & Long 1999).

CD158d contains two Ig domains and a single tyrosine based inhibition motif in the cytoplasmic domain. It also contains a charged arginine near the top of the transmembrane region which helps regulate the signal for cytotoxicity and cytokine secretion when engaged at the cell surface. These two features give CD158d both inhibitory and activating characteristics (Fauve & Long 2002).

This mAb#33 clone has been used in flow cytometry to examine CD158d levels upon infection of human extravillous cytotrophoblast cells with Toxoplasma gondii (Xu et al. 2013).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
NK3.3 cells and KIR2DL4-Ig fusion protein
Purity
>95% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/100
Functional Assays
Immunocytochemistry
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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References for CD158d antibody

  1. Kikuchi-maki, A. et al. (2003) KIR2DL4 is an IL-2-regulated NK cell receptor that exhibits limited expression in humans but triggers strong IFN-gamma production.
    J Immunol. 171 (7): 3415-25.
  2. Rajagopalan, S. & Long, E.O. (1999) A human histocompatibility leukocyte antigen (HLA)-G-specific receptor expressed on all natural killer cells.
    J Exp Med. 189 (7): 1093-100.
  3. Faure, M. & Long, E.O. (2002) KIR2DL4 (CD158d), an NK cell-activating receptor with inhibitory potential.
    J Immunol. 168 (12): 6208-14.
  4. Xu, X. et al. (2013) Toxoplasma gondii infection regulates the balance of activating and inhibitory receptors on decidual natural killer cells.
    PLoS One. 8 (2): e55432.

Synonyms
KIR2DL4
UniProt
Q99706
Entrez Gene
KIR2DL4
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