CD158b antibody | GL183

Mouse anti Human CD158b

Product Type
Monoclonal Antibody
Clone
GL183
Isotype
IgG1
Specificity
CD158b

Product Code Applications Pack Size List Price Your Price Qty
MCA1584
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SDS Safety Datasheet SDS
F IP 0.2 mg loader
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loader
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Mouse anti Human CD158b antibody, clone GL183 recognizes human Killer cell immunoglobulin-like receptor 2DL3, also known as CD158b, KIR-023GB, MHC class I NK cell receptor, p58 natural killer cell receptor clone CL-6 or Natural killer-associated transcript 2. CD158b is a 341 amino acid, ~58 kDa single pass type-1 transmembrane glycoprotein containing two Ig-like C2-type domains. expressed by a subset of NK cells.

This antibody also recognizes a ~50 kDa molecule in some NK clones, which is highly homologous to p58.2 in the extracellular domain, but has a shorter cytoplasmic tail (Moretta et al. 1985). Both molecules are members of the newly described natural killer cell receptor family.

CD158b functions as a receptor specific for HLA Class I molecules, including Cw3 and related HLA-C alleles. Mouse anti Human CD158b antibody, clone GL183 can restore the lysis by human NK clones of otherwise lysis protected targets expressing Cw3.

Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
NK cell clone E57 (Moretta et al. 1985).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the mouse P3UI myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Mouse IgG1 Negative Control

References for CD158b antibody

  1. Ciccone, E. et al. (1992) Evidence of a natural killer (NK) cell repertoire for (allo) antigen recognition: definition of five distinct NK-determined allospecificities in humans.
    J Exp Med. 175 (3): 709-18.
  2. Biassoni R et al. (1996) The human leukocyte antigen (HLA)-C-specific "activatory" or "inhibitory" natural killer cell receptors display highly homologous extracellular domains but differ in their transmembrane and intracytoplasmic portions.
    J Exp Med. 183 (2): 645-50.
  3. Long EO et al. (1996) Inhibitory MHC class I receptors on NK and T cells: a standard nomenclature.
    Immunol Today. 17 (2): 100.
  4. Moretta, A. et al. (1990) A novel surface antigen expressed by a subset of human CD3- CD16+ natural killer cells. Role in cell activation and regulation of cytolytic function.
    J Exp Med. 171 (3): 695-714.
  5. Moretta, A. et al. (1993) P58 molecules as putative receptors for major histocompatibility complex (MHC) class I molecules in human natural killer (NK) cells. Anti-p58 antibodies reconstitute lysis of MHC class I-protected cells in NK clones displaying different specificities.
    J Exp Med. 178 (2): 597-604.
  6. Moretta, A. et al. (1995) Existence of both inhibitory (p58) and activatory (p50) receptors for HLA-C molecules in human natural killer cells.
    J Exp Med. 182 (3): 875-84.
  7. Pridgeon, C. et al. (2003) Natural killer cells in the synovial fluid of rheumatoid arthritis patients exhibit a CD56bright,CD94bright,CD158negative phenotype.
    Rheumatology (Oxford). 42 (7): 870-8.
  8. Marget, M. et al. (2005) A HLA-Cw6 specific single-chain antibody fragment (scFv) recognizing a natural killer cell receptor epitope
    Mol Immunol. 42: 643-9.
  9. View The Latest Product References
  10. Poggi, A. et al. (2005) Regulation of gammadelta T cell survival by soluble HLA-I: involvement of CD8 and activating killer Ig-like receptors.
    Eur J Immunol. 35: 2670-8.
  11. Borhis, G. et al. (2013) A peptide antagonist disrupts NK cell inhibitory synapse formation.
    J Immunol. 190 (6): 2924-30.
  12. Poggi, A. et al. (2005) Patients with paroxysmal nocturnal hemoglobinuria have a high frequency of peripheral-blood T cells expressing activating isoforms of inhibiting superfamily receptors.
    Blood. 106: 2399-408.
  13. Valés-Gómez, M. et al. (2003) Expression of the UL16 glycoprotein of Human Cytomegalovirus protects the virus-infected cell from attack by natural killer cells.
    BMC Immunol. 4:4.
  14. Spaggiari, G.M. et al. (2002) Soluble HLA class I molecules induce natural killer cell apoptosis through the engagement of CD8: evidence for a negative regulation exerted by members of the inhibitory receptor superfamily.
    Blood. 99: 1706-14.
  15. Warren, H.S. et al. (2001) Biphasic response of NK cells expressing both activating and inhibitory killer Ig-like receptors.
    Int Immunol. 13: 1043-52.
  16. Ghio, M. et al. (2009) Soluble HLA-I-mediated secretion of TGF-beta1 by human NK cells and consequent down-regulation of anti-tumor cytolytic activity.
    Eur J Immunol. 39: 3459-68.
  17. Spaggiari, G.M. et al. (2003) IFN-gamma production in human NK cells through the engagement of CD8 by soluble or surface HLA class I molecules.
    Eur J Immunol. 33: 3049-59.
  18. Bachelet, I. et al. (2005) The inhibitory receptor IRp60 (CD300a) is expressed and functional on human mast cells.
    J Immunol. 175: 7989-95.
  19. Zimmer, J. et al. (1998) Activity and phenotype of natural killer cells in peptide transporter (TAP)-deficient patients (type I bare lymphocyte syndrome).
    J Exp Med. 187: 117-22.
  20. Castriconi, R.et al. (2009) NK cells recognize and kill human glioblastoma cells with stem cell-like properties.
    J Immunol. 182 (6): 3530-9.
  21. Ghio, M. et al. (2009) Soluble HLA-I-mediated secretion of TGF-beta1 by human NK cells and consequent down-regulation of anti-tumor cytolytic activity.
    Eur J Immunol. 39 (12): 3459-68.
  22. Naiyer, M.M. et al>. (2017) KIR2DS2 recognizes conserved peptides derived from viral helicases in the context of HLA-C.
    Sci Immunol. 2 (15) [Epub ahead of print].

Further Reading

  1. Moretta A et al. (1997) Major histocompatibility complex class I-specific receptors on human natural killer and T lymphocytes.
    Immunol Rev. 155: 105-17.

Synonyms
KIR2DL3
RRID
AB_2265256
UniProt
P43628
Entrez Gene
KIR2DL3
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0006955 immune response
GO:0050776 regulation of immune response
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