CD158b antibody | GL183
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Mouse anti Human CD158b
- Product Type
- Monoclonal Antibody
- Clone
- GL183
- Isotype
- IgG1
- Specificity
- CD158b
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| Mouse anti Human CD158b antibody, clone GL183 recognizes human Killer cell immunoglobulin-like receptor 2DL3, also known as CD158b, KIR-023GB, MHC class I NK cell receptor, p58 natural killer cell receptor clone CL-6 or Natural killer-associated transcript 2. CD158b is a 341 amino acid, ~58 kDa single pass type-1 transmembrane glycoprotein containing two Ig-like C2-type domains. expressed by a subset of NK cells. This antibody also recognizes a ~50 kDa molecule in some NK clones, which is highly homologous to p58.2 in the extracellular domain, but has a shorter cytoplasmic tail (Moretta et al. 1985). Both molecules are members of the newly described natural killer cell receptor family. CD158b functions as a receptor specific for HLA Class I molecules, including Cw3 and related HLA-C alleles. Mouse anti Human CD158b antibody, clone GL183 can restore the lysis by human NK clones of otherwise lysis protected targets expressing Cw3. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- NK cell clone E57 (Moretta et al. 1985).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the mouse P3UI myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/50 |
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD158b antibody
-
Ciccone, E. et al. (1992) Evidence of a natural killer (NK) cell repertoire for (allo) antigen recognition: definition of five distinct NK-determined allospecificities in humans.
J Exp Med. 175 (3): 709-18. -
Biassoni R et al. (1996) The human leukocyte antigen (HLA)-C-specific "activatory" or "inhibitory" natural killer cell receptors display highly homologous extracellular domains but differ in their transmembrane and intracytoplasmic portions.
J Exp Med. 183 (2): 645-50. -
Long EO et al. (1996) Inhibitory MHC class I receptors on NK and T cells: a standard nomenclature.
Immunol Today. 17 (2): 100. -
Moretta, A. et al. (1990) A novel surface antigen expressed by a subset of human CD3- CD16+ natural killer cells. Role in cell activation and regulation of cytolytic function.
J Exp Med. 171 (3): 695-714. -
Moretta, A. et al. (1993) P58 molecules as putative receptors for major histocompatibility complex (MHC) class I molecules in human natural killer (NK) cells. Anti-p58 antibodies reconstitute lysis of MHC class I-protected cells in NK clones displaying different specificities.
J Exp Med. 178 (2): 597-604. -
Moretta, A. et al. (1995) Existence of both inhibitory (p58) and activatory (p50) receptors for HLA-C molecules in human natural killer cells.
J Exp Med. 182 (3): 875-84. -
Pridgeon, C. et al. (2003) Natural killer cells in the synovial fluid of rheumatoid arthritis patients exhibit a CD56bright,CD94bright,CD158negative phenotype.
Rheumatology (Oxford). 42 (7): 870-8. -
Marget, M. et al. (2005) A HLA-Cw6 specific single-chain antibody fragment (scFv) recognizing a natural killer cell receptor epitope
Mol Immunol. 42: 643-9.
View The Latest Product References
-
Poggi, A. et al. (2005) Regulation of gammadelta T cell survival by soluble HLA-I: involvement of CD8 and activating killer Ig-like receptors.
Eur J Immunol. 35: 2670-8. -
Borhis, G. et al. (2013) A peptide antagonist disrupts NK cell inhibitory synapse formation.
J Immunol. 190 (6): 2924-30. -
Poggi, A. et al. (2005) Patients with paroxysmal nocturnal hemoglobinuria have a high frequency of peripheral-blood T cells expressing activating isoforms of inhibiting superfamily receptors.
Blood. 106: 2399-408. -
Valés-Gómez, M. et al. (2003) Expression of the UL16 glycoprotein of Human Cytomegalovirus protects the virus-infected cell from attack by natural killer cells.
BMC Immunol. 4:4. -
Spaggiari, G.M. et al. (2002) Soluble HLA class I molecules induce natural killer cell apoptosis through the engagement of CD8: evidence for a negative regulation exerted by members of the inhibitory receptor superfamily.
Blood. 99: 1706-14. -
Warren, H.S. et al. (2001) Biphasic response of NK cells expressing both activating and inhibitory killer Ig-like receptors.
Int Immunol. 13: 1043-52. -
Ghio, M. et al. (2009) Soluble HLA-I-mediated secretion of TGF-beta1 by human NK cells and consequent down-regulation of anti-tumor cytolytic activity.
Eur J Immunol. 39: 3459-68. -
Spaggiari, G.M. et al. (2003) IFN-gamma production in human NK cells through the engagement of CD8 by soluble or surface HLA class I molecules.
Eur J Immunol. 33: 3049-59. -
Bachelet, I. et al. (2005) The inhibitory receptor IRp60 (CD300a) is expressed and functional on human mast cells.
J Immunol. 175: 7989-95. -
Zimmer, J. et al. (1998) Activity and phenotype of natural killer cells in peptide transporter (TAP)-deficient patients (type I bare lymphocyte syndrome).
J Exp Med. 187: 117-22. -
Castriconi, R.et al. (2009) NK cells recognize and kill human glioblastoma cells with stem cell-like properties.
J Immunol. 182 (6): 3530-9. -
Ghio, M. et al. (2009) Soluble HLA-I-mediated secretion of TGF-beta1 by human NK cells and consequent down-regulation of anti-tumor cytolytic activity.
Eur J Immunol. 39 (12): 3459-68. -
Naiyer, M.M. et al>. (2017) KIR2DS2 recognizes conserved peptides derived from viral helicases in the context of HLA-C.
Sci Immunol. 2 (15) [Epub ahead of print].
Further Reading
-
Moretta A et al. (1997) Major histocompatibility complex class I-specific receptors on human natural killer and T lymphocytes.
Immunol Rev. 155: 105-17.
- Synonyms
- KIR2DL3
- RRID
- AB_2265256
- UniProt
- P43628
- Entrez Gene
- KIR2DL3
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006955 immune response
- GO:0050776 regulation of immune response
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