CD158b antibody | GL183

This antibody also recognizes a ~50 kDa molecule in some NK clones, which is highly homologous to p58.2 in the extracellular domain, but has a shorter cytoplasmic tail (Moretta et al. 1985). Both molecules are members of the newly described natural killer cell receptor family.
CD158b functions as a receptor specific for HLA Class I molecules, including Cw3 and related HLA-C alleles. Mouse anti Human CD158b antibody, clone GL183 can restore the lysis by human NK clones of otherwise lysis protected targets expressing Cw3.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- NK cell clone E57 (Moretta et al. 1985).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the mouse P3UI myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P43628
- Entrez Gene
- KIR2DL3
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006955 immune response
- GO:0050776 regulation of immune response
- Regulatory
- For research purposes only
Applications of CD158b antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/10 | 1/50 | |
Immunoprecipitation |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
![]() |
Product Specific References
References for CD158b antibody
-
Moretta, A. et al. (1990) A novel surface antigen expressed by a subset of human CD3- CD16+ natural killer cells. Role in cell activation and regulation of cytolytic function.
J Exp Med. 171 (3): 695-714. -
Moretta, A. et al. (1993) P58 molecules as putative receptors for major histocompatibility complex (MHC) class I molecules in human natural killer (NK) cells. Anti-p58 antibodies reconstitute lysis of MHC class I-protected cells in NK clones displaying different specificities.
J Exp Med. 178 (2): 597-604. -
Moretta, A. et al. (1995) Existence of both inhibitory (p58) and activatory (p50) receptors for HLA-C molecules in human natural killer cells.
J Exp Med. 182 (3): 875-84. -
Pridgeon, C. et al. (2003) Natural killer cells in the synovial fluid of rheumatoid arthritis patients exhibit a CD56bright,CD94bright,CD158negative phenotype.
Rheumatology (Oxford). 42 (7): 870-8. -
Marget, M. et al. (2005) A HLA-Cw6 specific single-chain antibody fragment (scFv) recognizing a natural killer cell receptor epitope
Mol Immunol. 42: 643-9. -
Poggi, A. et al. (2005) Regulation of gammadelta T cell survival by soluble HLA-I: involvement of CD8 and activating killer Ig-like receptors.
Eur J Immunol. 35: 2670-8. -
Borhis, G. et al. (2013) A peptide antagonist disrupts NK cell inhibitory synapse formation.
J Immunol. 190 (6): 2924-30. -
Poggi, A. et al. (2005) Patients with paroxysmal nocturnal hemoglobinuria have a high frequency of peripheral-blood T cells expressing activating isoforms of inhibiting superfamily receptors.
Blood. 106: 2399-408. -
Valés-Gómez, M. et al. (2003) Expression of the UL16 glycoprotein of Human Cytomegalovirus protects the virus-infected cell from attack by natural killer cells.
BMC Immunol. 4:4. -
Spaggiari, G.M. et al. (2002) Soluble HLA class I molecules induce natural killer cell apoptosis through the engagement of CD8: evidence for a negative regulation exerted by members of the inhibitory receptor superfamily.
Blood. 99: 1706-14. -
Warren, H.S. et al. (2001) Biphasic response of NK cells expressing both activating and inhibitory killer Ig-like receptors.
Int Immunol. 13: 1043-52. -
Ghio, M. et al. (2009) Soluble HLA-I-mediated secretion of TGF-beta1 by human NK cells and consequent down-regulation of anti-tumor cytolytic activity.
Eur J Immunol. 39: 3459-68. -
Spaggiari, G.M. et al. (2003) IFN-gamma production in human NK cells through the engagement of CD8 by soluble or surface HLA class I molecules.
Eur J Immunol. 33: 3049-59. -
Bachelet, I. et al. (2005) The inhibitory receptor IRp60 (CD300a) is expressed and functional on human mast cells.
J Immunol. 175: 7989-95. -
Zimmer, J. et al. (1998) Activity and phenotype of natural killer cells in peptide transporter (TAP)-deficient patients (type I bare lymphocyte syndrome).
J Exp Med. 187: 117-22. -
Castriconi, R.et al. (2009) NK cells recognize and kill human glioblastoma cells with stem cell-like properties.
J Immunol. 182 (6): 3530-9. -
Ghio, M. et al. (2009) Soluble HLA-I-mediated secretion of TGF-beta1 by human NK cells and consequent down-regulation of anti-tumor cytolytic activity.
Eur J Immunol. 39 (12): 3459-68. -
Naiyer, M.M. et al>. (2017) KIR2DS2 recognizes conserved peptides derived from viral helicases in the context of HLA-C.
Sci Immunol. 2 (15) [Epub ahead of print].
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra