CD158b antibody | GL183

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Mouse anti Human CD158b

Product Type: Monoclonal Antibody
Clone: GL183
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1584	F IP			0.2 mg

Mouse anti Human CD158b antibody, clone GL183 recognizes human Killer cell immunoglobulin-like receptor 2DL3, also known as CD158b, KIR-023GB, MHC class I NK cell receptor, p58 natural killer cell receptor clone CL-6 or Natural killer-associated transcript 2. CD158b is a 341 amino acid, ~58 kDa single pass type-1 transmembrane glycoprotein containing two Ig-like C2-type domains. expressed by a subset of NK cells.

This antibody also recognizes a ~50 kDa molecule in some NK clones, which is highly homologous to p58.2 in the extracellular domain, but has a shorter cytoplasmic tail (Moretta et al. 1985). Both molecules are members of the newly described natural killer cell receptor family.

CD158b functions as a receptor specific for HLA Class I molecules, including Cw3 and related HLA-C alleles. Mouse anti Human CD158b antibody, clone GL183 can restore the lysis by human NK clones of otherwise lysis protected targets expressing Cw3.

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin
Immunogen: NK cell clone E57 (Moretta et al. 1985).
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunized Balb/c mice were fused with cells of the mouse P3UI myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P43628 Related reagents
Entrez Gene: KIR2DL3 Related reagents
GO Terms: GO:0005515 protein binding; GO:0003823 antigen binding; GO:0004872 receptor activity; GO:0005887 integral to plasma membrane; GO:0006955 immune response; GO:0050776 regulation of immune response
Regulatory: For research purposes only

Applications of CD158b antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		1/10	1/50
Immunoprecipitation

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Product Specific References

References for CD158b antibody

Moretta, A. et al. (1990) A novel surface antigen expressed by a subset of human CD3- CD16+ natural killer cells. Role in cell activation and regulation of cytolytic function.
J Exp Med. 171 (3): 695-714.
Moretta, A. et al. (1993) P58 molecules as putative receptors for major histocompatibility complex (MHC) class I molecules in human natural killer (NK) cells. Anti-p58 antibodies reconstitute lysis of MHC class I-protected cells in NK clones displaying different specificities.
J Exp Med. 178 (2): 597-604.
Moretta, A. et al. (1995) Existence of both inhibitory (p58) and activatory (p50) receptors for HLA-C molecules in human natural killer cells.
J Exp Med. 182 (3): 875-84.
Pridgeon, C. et al. (2003) Natural killer cells in the synovial fluid of rheumatoid arthritis patients exhibit a CD56bright,CD94bright,CD158negative phenotype.
Rheumatology (Oxford). 42 (7): 870-8.
Marget, M. et al. (2005) A HLA-Cw6 specific single-chain antibody fragment (scFv) recognizing a natural killer cell receptor epitope
Mol Immunol. 42: 643-9.
Poggi, A. et al. (2005) Regulation of gammadelta T cell survival by soluble HLA-I: involvement of CD8 and activating killer Ig-like receptors.
Eur J Immunol. 35: 2670-8.
Borhis, G. et al. (2013) A peptide antagonist disrupts NK cell inhibitory synapse formation.
J Immunol. 190 (6): 2924-30.
Poggi, A. et al. (2005) Patients with paroxysmal nocturnal hemoglobinuria have a high frequency of peripheral-blood T cells expressing activating isoforms of inhibiting superfamily receptors.
Blood. 106: 2399-408.
Valés-Gómez, M. et al. (2003) Expression of the UL16 glycoprotein of Human Cytomegalovirus protects the virus-infected cell from attack by natural killer cells.
BMC Immunol. 4:4.
Spaggiari, G.M. et al. (2002) Soluble HLA class I molecules induce natural killer cell apoptosis through the engagement of CD8: evidence for a negative regulation exerted by members of the inhibitory receptor superfamily.
Blood. 99: 1706-14.
Warren, H.S. et al. (2001) Biphasic response of NK cells expressing both activating and inhibitory killer Ig-like receptors.
Int Immunol. 13: 1043-52.
Ghio, M. et al. (2009) Soluble HLA-I-mediated secretion of TGF-beta1 by human NK cells and consequent down-regulation of anti-tumor cytolytic activity.
Eur J Immunol. 39: 3459-68.
Spaggiari, G.M. et al. (2003) IFN-gamma production in human NK cells through the engagement of CD8 by soluble or surface HLA class I molecules.
Eur J Immunol. 33: 3049-59.
Bachelet, I. et al. (2005) The inhibitory receptor IRp60 (CD300a) is expressed and functional on human mast cells.
J Immunol. 175: 7989-95.
Zimmer, J. et al. (1998) Activity and phenotype of natural killer cells in peptide transporter (TAP)-deficient patients (type I bare lymphocyte syndrome).
J Exp Med. 187: 117-22.
Castriconi, R,. et al. (2009) NK cells recognize and kill human glioblastoma cells with stem cell-like properties.
J Immunol. 182: 3530-9.
Ghio, M. et al. (2009) Soluble HLA-I-mediated secretion of TGF-beta1 by human NK cells and consequent down-regulation of anti-tumor cytolytic activity.
Eur J Immunol. 39 (12): 3459-68.
Naiyer, M.M. et al. (2017) KIR2DS2 recognizes conserved peptides derived from viral helicases in the context of HLA-C.
Sci Immunol. 2 (15) [Epub ahead of print].

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