CD151 antibody | 11G5a

Mouse anti Human CD151

Product Type
Monoclonal Antibody
Clone
11G5a
Isotype
IgG1
Specificity
CD151

Product Code Applications Pack Size List Price Your Price Qty
MCA1856F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
MCA1856FT
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SDS Safety Datasheet SDS
F 25 µg loader
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loader
MCA1856GA
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SDS Safety Datasheet SDS
C E F IF IP P * WB * 0.1 mg loader
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loader
MCA1856
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F IF IP P * WB * 0.2 mg loader
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loader
MCA1856T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F IF IP P * WB * 25 µg loader
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loader
MCA1856PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD151 antibody, clone 11G5a recognizes the human CD151 cell surface antigen, also known as PETA-3. CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen (Crew et al. 2004).

It should be noted that CD151 is very closely associated with the alpha3 beta1 integrin in cells and co-immunoprecipitation may occur even in quite stringent conditions (Yauch. et al. 1998).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
MCA1856GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA1856, MCA1856T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1 1/100
Immunoprecipitation
Western Blotting 2
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
  2. 2 Clone 11G5a recognises human CD151 under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Histology Positive Control Tissue
Tonsil/spleen.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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loader
Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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loader
Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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loader
Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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loader
Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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loader
Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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loader
Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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loader
Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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loader
Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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loader
Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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loader
Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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loader
Description Mouse IgG1 Negative Control

References for CD151 antibody

  1. Karamatic Crew, V. et al. (2004) CD151, the first member of the tetraspanin (TM4) superfamily detected on erythrocytes, is essential for the correct assembly of human basement membranes in kidney and skin.
    Blood. 104 (8): 2217-23.
  2. Ke, A.W. et al. (2011) CD151 amplifies signaling by integrin α6β1 to PI3K and induces the epithelial-mesenchymal transition in HCC cells.
    Gastroenterology. 140: 1629-41.e15.
  3. Zheng, Z. & Liu, Z. (2006) CD151 gene delivery activates PI3K/Akt pathway and promotes neovascularization after myocardial infarction in rats.
    Mol Med. 12 (9-10): 214-20.
  4. Zheng, Z. & Liu, Z. (2007) CD151 gene delivery increases eNOS activity and induces ECV304 migration, proliferation and tube formation.
    Acta Pharmacol Sin. 28 (1): 66-72.
  5. Franco, M. et al. (2010) The tetraspanin CD151 is required for Met-dependent signaling and tumor cell growth.
    J Biol Chem. 285 (50): 38756-64.
  6. Hasegawa, M. et al. (2007) CD151 dynamics in carcinoma-stroma interaction: integrin expression, adhesion strength and proteolytic activity.
    Lab Invest. 87: 882-92.
  7. Spoden, G. et al. (2008) Clathrin- and caveolin-independent entry of human papillomavirus type 16--involvement of tetraspanin-enriched microdomains (TEMs).
    PLoS One. 3: e3313.
  8. Huang, X.Y. et al. (2010) Overexpression of CD151 as an adverse marker for intrahepatic cholangiocarcinoma patients.
    Cancer. 116: 5440-51.
  9. View The Latest Product References
  10. Ke, A.W. et al. (2009) Role of overexpression of CD151 and/or c-Met in predicting prognosis of hepatocellular carcinoma.
    Hepatology. 49: 491-503.
  11. Devbhandari, R.P. et al. (2011) Profiling of the tetraspanin CD151 web and conspiracy of CD151/integrin β1 complex in the progression of hepatocellular carcinoma.
    PLoS One. 6: e24901.
  12. Shi, G.M. et al. (2010) CD151 modulates expression of matrix metalloproteinase 9 and promotes neoangiogenesis and progression of hepatocellular carcinoma.
    Hepatology. 52: 183-96.
  13. Nishiuchi, R. et al. (2005) Potentiation of the ligand-binding activity of integrin alpha3beta1 via association with tetraspanin CD151.
    Proc Natl Acad Sci U S A.102: 1939-44.
  14. Qiao, Y. et al. (2017) CD151, a laminin receptor showing increased expression in asthmatic patients, contributes to airway hyperresponsiveness through calcium signaling.
    J Allergy Clin Immunol. 139 (1): 82-92.e5.
  15. Hochdorfer, D. et al. (2016) Tetraspanin CD151 Promotes Initial Events in Human Cytomegalovirus Infection.
    J Virol. 90 (14): 6430-42.
  16. Wadkin, J.C.R. et al. (2017) CD151 supports VCAM-1-mediated lymphocyte adhesion to liver endothelium and is upregulated in chronic liver disease and hepatocellular carcinoma.
    Am J Physiol Gastrointest Liver Physiol. 313 (2): G138-G149.
  17. Burkard, C. et al. (2017) Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function.
    PLoS Pathog. 13 (2): e1006206.
  18. Yang, Y.M. et al. (2013) Overexpression of CD151 predicts prognosis in patients with resected gastric cancer.
    PLoS One. 8 (3): e58990.

Further Reading

  1. Kwon, M.J. et al. (2012) Clinical significance of CD151 overexpression in subtypes of invasive breast cancer.
    Br J Cancer. 106: 923-30.
  2. Yauch, R.L. et al. (1998) Highly stoichiometric, stable, and specific association of integrin alpha3beta1 with CD151 provides a major link to phosphatidylinositol 4-kinase, and may regulate cell migration.
    Mol Biol Cell. 9 (10): 2751-65.

Flow Cytometry

Immunofluorescence

Immunoprecipitation

RRID
AB_323247
UniProt
P48509
Entrez Gene
CD151
GO Terms
GO:0005515 protein binding
GO:0007155 cell adhesion
GO:0005829 cytosol
GO:0005887 integral to plasma membrane
GO:0005624 membrane fraction
GO:0031581 hemidesmosome assembly
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