CD15 antibody | MCS-1
Mouse anti Human CD15
- Product Type
- Monoclonal Antibody
- Clone
- MCS-1
- Isotype
- IgG3
- Specificity
- CD15
Antibody Quality and Performance Guarantee
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| Mouse anti Human CD15 antibody, clone MCS-1 recognizes human CD15, otherwise known as Lewis X and stage-specfic embryonic antigen-1 (SSEA-1). The CD15 antigen is itself a terminal trisaccharide, Gal-beta1-4 (Fuc-alpha1-3) GlcNAc (3-FAL), which is found on a variety of glycoproteins and glycolipids. CD15 is predominately expressed by peripheral blood granulocytes and various epithelial tissues. It plays an important role in cell adhesion and has been used as a marker for diagnosis of Hodgkin’s lymphoma. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 20ul of the suggested working dilution to label 1x106 cells in 100ul.
Source Reference
-
Sagawa, K. et al. (1995) Murine monoclonal antibodies (MCS-1 and MCS-2) reactive with human myeloid leukemia cells.
Kurume Med J. 42 (3): 177-85.
References for CD15 antibody
-
Drexler, H.G. & Minowada, J. (1986) The use of monoclonal antibodies for the identification and classification of acute myeloid leukemias.
Leuk Res. 10 (3): 279-90. -
Oxford University Press. (1998) .
Leucocyte Typing IV. p798-810. -
Chimori, Y. & Minowada, J. (1991) Identification and characterization of a colon-cancer-associated antigen expressed on leukemia-lymphoma cell lines.
Int J Cancer. 47 (3): 426-30.
- Synonyms
- Lewis X
- RRID
- AB_10672021
MCA5669
100311R
165099
169551
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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