CD146 antibody | OJ79c

100% Secure


Mouse anti Human CD146:Alexa Fluor® 647

Mouse anti Human CD146:Preservative Free

Mouse anti Human CD146:FITC

Mouse anti Human CD146

Product Type
Monoclonal Antibody
Clone
OJ79c
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2141A647T F 25 Tests/0.25ml
MCA2141A647 F 100 Tests/1ml
MCA2141XZ C E F IF 1 mg
MCA2141FT F 25 µg
MCA2141F F 0.1 mg
MCA2141T C E F IF 25 µg
MCA2141 C E F IF 0.2 mg
Mouse anti Human CD146 antibody, clone OJ79c recognizes human Cell surface glycoprotein MUC18, also known as CD146, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule (MCAM) or S-endo 1 endothelial-associated antigen. CD146 is a 646 amino acid single pass type 1 transmembrane glycoprotein with a calculated molecular mass of ~72 kDa. However due to extensive N-linked glycosylation CD146 migrates in polyacrylamide gels with an apparent molecular mass of ~118 kDa. CD146 is a member of the immunoglobulin superfamily bearing 2 V-type Ig-like and 3 C-type Ig-like domains. CD146 is expressed by all endothelial cells and by melanoma cells and appears to act as an adhesion molecule (UniProt: P43121). Expression in melanoma may be linked to tumor progression (Lehmann et al. 1989).

Mouse anti Human CD146 antibody, clone OJ79c is highly expressed on pericytes and has been utilized for the identification of perivascular mesenchymal precursor cells from cardiac muscle using flow cytometry (Chen et al. 2014).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Dog
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
Pack Size: 25 µg
0.09%Sodium Azide
Pack Size: 0.2 mg
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Recombinant human MUC18 (D1-D5) Fc protein.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse Sp2/0 Ag.14 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P43121 Related reagents
Entrez Gene
MCAM Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0009653 anatomical structure morphogenesis
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD146 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
ELISA
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen
Flow Cytometry Neat 1/10
ELISA
Flow Cytometry 1/10 1/50
Immunofluorescence
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Melanoma
Histology Positive Control Tissue
Melanoma

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 100 Tests/1ml F
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for CD146 antibody

  1. Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
    PLoS One. 7: e35577.
  2. Crisan, M. et al. (2008) A perivascular origin for mesenchymal stem cells in multiple human organs.
    Cell Stem Cell. 3: 301-13.
  3. Iohara, K. et al. (2008) A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp.
    Stem Cells. 26 (9): 2408-18.
  4. Park, T.S. et al. (2010) Placental Perivascular Cells for Human Muscle Regeneration.
    Stem Cells Dev. 20: 451-63.
  5. Smith, K. et al. (2011) Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.
    Immunology. 132: 256-65.
  6. James, A.W. et al. (2012) Perivascular stem cells: a prospectively purified mesenchymal stem cell population for bone tissue engineering.
    Stem Cells Transl Med. 1 (6): 510-9.
  7. Wassmer, S.C. et al. (2011) Vascular endothelial cells cultured from patients with cerebral or uncomplicated malaria exhibit differential reactivity to TNF.
    Cell Microbiol. 13: 198-209.
  8. Lee, J.H. et al. (2012) Generation of osteogenic construct using periosteal-derived osteoblasts and polydioxanone/pluronic F127 scaffold with periosteal-derived CD146 positive endothelial-like cells.
    J Biomed Mater Res A.101: 942-53.
  9. Boneberg, E.M. et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process.
    Microvasc Res. 78: 325-31.
  10. Murakami, M. et al. (2012) Identification of novel function of vimentin for quality standard for regenerated pulp tissue.
    J Endod. 38: 920-6.
  11. Nielsen, C.T. et al. (2011) Distinct features of circulating microparticles and their relationship to clinical manifestations in systemic lupus erythematosus.
    Arthritis Rheum. 63: 3067-77.
  12. Zhang, Y. et al. (2009) The Impact of Proliferative Potential of Umbilical Cord–Derived Endothelial Progenitor Cells and Hypoxia on Vascular Tubule Formation In Vitro.
    Stem Cells Dev. 18: 359-75.
  13. Iversen, L.V. et al. (2013) A heparin-based method for flow cytometric analysis of microparticles directly from platelet-poor plasma in calcium containing buffer.
    J Immunol Methods. 388 (1-2): 49-59.
  14. Meireles, A.L. et al. (2011) Increased levels of circulating endothelial progenitor cells in human T-cell lymphotropic virus type I carriers.
    Arch Med Res. 42: 34-7.
  15. Iversen, L.V. et al. (2013) Circulating microparticles and plasma levels of soluble E- and P-selectins in patients with systemic sclerosis.
    Scand J Rheumatol. 42 (6): 473-82.
  16. Murakami, M. et al. (2013) The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential.
    Biomaterials. pii: S0142-9612(13)00942-3.
  17. Ruetze, M. et al. (2013) A novel niche for skin derived precursors in non-follicular skin.
    J Dermatol Sci. 69: 132-9.
  18. Dokić, J. et al. (2013) Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells.
    Eur J Immunol. 43: 1862-72.
  19. Chen, W.C. et al. (2015) Human myocardial pericytes: multipotent mesodermal precursors exhibiting cardiac specificity.
    Stem Cells. 33 (2): 557-73.
  20. Iversen, L.V. et al. (2015) Cross-sectional study of soluble selectins, fractions of circulating microparticles and their relationship to lung and skin involvement in systemic sclerosis.
    BMC Musculoskelet Disord. 16: 191.
  21. Tawonsawatruk T et al. (2016) Adipose derived pericytes rescue fractures from a failure of healing - non-union.
    Sci Rep. 6: 22779.
  22. Boissier, R. et al. (2016) Histologic and urodynamic effects of autologous stromal vascular fraction extracted from fat tissue with minimal ex vivo manipulation on a porcine model of intrinsic sphincter deficiency
    The Journal of Urology. Jun 2 [Epub ahead of print]
  23. Collison, J.L. et al. (2015) Heterogeneity in the Locomotory Behavior of Human Monocyte Subsets over Human Vascular Endothelium In Vitro.
    J Immunol. 195 (3): 1162-70.
  24. Stefanska, A. et al. (2016) Human kidney pericytes produce renin.
    Kidney Int. 90 (6): 1251-61.
  25. Lapin, M. et al. (2016) MINDEC-An Enhanced Negative Depletion Strategy for Circulating Tumour Cell Enrichment.
    Sci Rep. 6: 28929.
  26. Muerza-Cascante, M.L. et al. (2016) Endosteal-like extracellular matrix expression on melt electrospun written scaffolds.
    Acta Biomater. pii: S1742-7061(16)30706-1.
  27. Eliasberg, C.D. et al. (2017) Perivascular Stem Cells Diminish Muscle Atrophy Following Massive Rotator Cuff Tears in a Small Animal Model.
    J Bone Joint Surg Am. 99 (4): 331-341.
  28. James, A.W. et al. (2017) Isolation and characterization of canine perivascular stem/stromal cells for bone tissue engineering.
    PLoS One. 12 (5): e0177308.
  29. Shen J et al. (2017) Effects of WNT3A and WNT16 on the Osteogenic and Adipogenic Differentiation of Perivascular Stem/Stromal Cells.
    Tissue Eng Part A. May 22 [Epub ahead of print]
  30. Lapin, M. et al. (2017) Single-cell mRNA profiling reveals transcriptional heterogeneity among pancreatic circulating tumour cells.
    BMC Cancer. 17 (1): 390.
  31. Esteves, C.L. et al. (2017) Isolation and characterization of equine native MSC populations.
    Stem Cell Res Ther. 8 (1): 80.
  32. Gaceb, A. et al. (2017) Pericytes secrete pro-regenerative molecules in response to platelet-derived growth factor-BB.
    J Cereb Blood Flow Metab. : 271678X17719645.

Further Reading

  1. Kuzu, I. et al. (1993) Expression of adhesion molecules on the endothelium of normal tissue vessels and vascular tumors.
    Lab Invest. 69 (3): 322-8.
  2. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.