CD146 antibody | OJ79c

Mouse anti Human CD146

Product Type
Monoclonal Antibody
Clone
OJ79c
Isotype
IgG1
Specificity
CD146

Product Code Applications Pack Size List Price Your Price Qty
MCA2141T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F IF 25 µg loader
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loader
MCA2141
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SDS Safety Datasheet SDS
C E F IF 0.2 mg loader
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loader
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Mouse anti Human CD146 antibody, clone OJ79c recognizes human Cell surface glycoprotein MUC18, also known as CD146, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule (MCAM) or S-endo 1 endothelial-associated antigen. CD146 is a 646 amino acid single pass type 1 transmembrane glycoprotein with a calculated molecular mass of ~72 kDa. However due to extensive N-linked glycosylation CD146 migrates in polyacrylamide gels with an apparent molecular mass of ~118 kDa. CD146 is a member of the immunoglobulin superfamily bearing 2 V-type Ig-like and 3 C-type Ig-like domains. CD146 is expressed by all endothelial cells and by melanoma cells and appears to act as an adhesion molecule (UniProt: P43121). Expression in melanoma may be linked to tumor progression (Lehmann et al. 1989).

Mouse anti Human CD146 antibody, clone OJ79c is highly expressed on pericytes and has been utilized for the identification of perivascular mesenchymal precursor cells from cardiac muscle using flow cytometry (Chen et al. 2014).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Dog
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 25 µg
0.09%Sodium Azide
Pack Size: 0.2 mg
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant human MUC18 (D1-D5) Fc protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse Sp2/0 Ag.14 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/10 1/50
Immunofluorescence
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Melanoma

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

References for CD146 antibody

  1. Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
    PLoS One. 7: e35577.
  2. Crisan, M. et al. (2008) A perivascular origin for mesenchymal stem cells in multiple human organs.
    Cell Stem Cell. 3: 301-13.
  3. Iohara, K. et al. (2008) A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp.
    Stem Cells. 26 (9): 2408-18.
  4. Park, T.S. et al. (2010) Placental Perivascular Cells for Human Muscle Regeneration.
    Stem Cells Dev. 20: 451-63.
  5. Smith, K. et al. (2011) Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.
    Immunology. 132: 256-65.
  6. James, A.W. et al. (2012) Perivascular stem cells: a prospectively purified mesenchymal stem cell population for bone tissue engineering.
    Stem Cells Transl Med. 1 (6): 510-9.
  7. Wassmer, S.C. et al. (2011) Vascular endothelial cells cultured from patients with cerebral or uncomplicated malaria exhibit differential reactivity to TNF.
    Cell Microbiol. 13: 198-209.
  8. Lee, J.H. et al. (2012) Generation of osteogenic construct using periosteal-derived osteoblasts and polydioxanone/pluronic F127 scaffold with periosteal-derived CD146 positive endothelial-like cells.
    J Biomed Mater Res A.101: 942-53.
  9. View The Latest Product References
  10. Boneberg, E.M. et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process.
    Microvasc Res. 78: 325-31.
  11. Murakami, M. et al. (2012) Identification of novel function of vimentin for quality standard for regenerated pulp tissue.
    J Endod. 38: 920-6.
  12. Nielsen, C.T. et al. (2011) Distinct features of circulating microparticles and their relationship to clinical manifestations in systemic lupus erythematosus.
    Arthritis Rheum. 63: 3067-77.
  13. Zhang, Y. et al. (2009) The Impact of Proliferative Potential of Umbilical Cord–Derived Endothelial Progenitor Cells and Hypoxia on Vascular Tubule Formation In Vitro.
    Stem Cells Dev. 18: 359-75.
  14. Iversen, L.V. et al. (2013) A heparin-based method for flow cytometric analysis of microparticles directly from platelet-poor plasma in calcium containing buffer.
    J Immunol Methods. 388 (1-2): 49-59.
  15. Meireles, A.L. et al. (2011) Increased levels of circulating endothelial progenitor cells in human T-cell lymphotropic virus type I carriers.
    Arch Med Res. 42: 34-7.
  16. Iversen, L.V. et al. (2013) Circulating microparticles and plasma levels of soluble E- and P-selectins in patients with systemic sclerosis.
    Scand J Rheumatol. 42 (6): 473-82.
  17. Murakami, M. et al. (2013) The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential.
    Biomaterials. pii: S0142-9612(13)00942-3.
  18. Ruetze, M. et al. (2013) A novel niche for skin derived precursors in non-follicular skin.
    J Dermatol Sci. 69: 132-9.
  19. Dokić, J. et al. (2013) Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells.
    Eur J Immunol. 43: 1862-72.
  20. Chen, W.C. et al. (2015) Human myocardial pericytes: multipotent mesodermal precursors exhibiting cardiac specificity.
    Stem Cells. 33 (2): 557-73.
  21. Iversen, L.V. et al. (2015) Cross-sectional study of soluble selectins, fractions of circulating microparticles and their relationship to lung and skin involvement in systemic sclerosis.
    BMC Musculoskelet Disord. 16: 191.
  22. Tawonsawatruk T et al. (2016) Adipose derived pericytes rescue fractures from a failure of healing - non-union.
    Sci Rep. 6: 22779.
  23. Boissier, R. et al. (2016) Histologic and urodynamic effects of autologous stromal vascular fraction extracted from fat tissue with minimal ex vivo manipulation on a porcine model of intrinsic sphincter deficiency
    J Urology. Jun 2 [Epub ahead of print]
  24. Esteves, C.L. et al. (2017) Equine Mesenchymal Stromal Cells Retain a Pericyte-Like Phenotype.
    Stem Cells Dev. 26 (13): 964-72.
  25. Stefanska, A. et al. (2016) Human kidney pericytes produce renin.
    Kidney Int. 90 (6): 1251-61.
  26. Lapin, M. et al. (2016) MINDEC-An Enhanced Negative Depletion Strategy for Circulating Tumour Cell Enrichment.
    Sci Rep. 6: 28929.
  27. Muerza-Cascante, M.L. et al. (2016) Endosteal-like extracellular matrix expression on melt electrospun written scaffolds.
    Acta Biomater. pii: S1742-7061(16)30706-1.
  28. Eliasberg, C.D. et al. (2017) Perivascular Stem Cells Diminish Muscle Atrophy Following Massive Rotator Cuff Tears in a Small Animal Model.
    J Bone Joint Surg Am. 99 (4): 331-41.
  29. James, A.W. et al. (2017) Isolation and characterization of canine perivascular stem/stromal cells for bone tissue engineering.
    PLoS One. 12 (5): e0177308.
  30. Shen, J. et al. (2018) Effects of WNT3A and WNT16 on the Osteogenic and Adipogenic Differentiation of Perivascular Stem/Stromal Cells.
    Tissue Eng Part A. 24 (1-2): 68-80.
  31. Lapin, M. et al. (2017) Single-cell mRNA profiling reveals transcriptional heterogeneity among pancreatic circulating tumour cells.
    BMC Cancer. 17 (1): 390.
  32. Esteves, C.L. et al. (2017) Isolation and characterization of equine native MSC populations.
    Stem Cell Res Ther. 8 (1): 80.
  33. Gaceb, A. et al. (2017) Pericytes secrete pro-regenerative molecules in response to platelet-derived growth factor-BB.
    J Cereb Blood Flow Metab. : 271678X17719645.
  34. Noda, S. et al. (2019) Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation.
    Sci Rep. 9 (1): 5430.
  35. Gaceb, A. et al. (2020) An In Vitro. Partial Lesion Model of Differentiated Human Mesencephalic Neurons: Effect of Pericyte Secretome on Phenotypic Markers.
    J Mol Neurosci. 70 (11): 1914-25.
  36. Stefanska, A. et al. (2021) Role of Pericytes in the Development of the Renin/Angiotensin System: Induction of Functional Renin in Cultures of Pericytes.
    Methods Mol Biol. 2235: 169-180.
  37. Orikasa, S. et al. (2022) Hypoxia-inducible factor 1α induces osteo/odontoblast differentiation of human dental pulp stem cells via Wnt/β-catenin transcriptional cofactor BCL9.
    Sci Rep. 12 (1): 682.

Further Reading

  1. Kuzu, I. et al. (1993) Expression of adhesion molecules on the endothelium of normal tissue vessels and vascular tumors.
    Lab Invest. 69 (3): 322-8.
  2. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Flow Cytometry

Immunofluorescence

Synonyms
MUC18
RRID
AB_324068
UniProt
P43121
Entrez Gene
MCAM
GO Terms
GO:0005886 plasma membrane
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0009653 anatomical structure morphogenesis
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