CD146 antibody | OJ79c


















Mouse anti Human CD146:Alexa Fluor® 647
Mouse anti Human CD146:Preservative Free
Mouse anti Human CD146:FITC
Mouse anti Human CD146
- Product Type
- Monoclonal Antibody
- Clone
- OJ79c
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA2141A647T | F | 25 Tests/0.25ml |
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MCA2141A647 | F | 100 Tests/1ml |
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MCA2141XZ | C E F IF | 1 mg |
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MCA2141FT | F | 25 µg |
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MCA2141F | F | 0.1 mg |
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MCA2141T | C E F IF | 25 µg |
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MCA2141 | C E F IF | 0.2 mg |
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Mouse anti Human CD146 antibody, clone OJ79c is highly expressed on pericytes and has been utilized for the identification of perivascular mesenchymal precursor cells from cardiac muscle using flow cytometry (Chen et al. 2014).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig Dog - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- Pack Size: 25 µg
0.09% Sodium Azide Pack Size: 0.2 mg0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Recombinant human MUC18 (D1-D5) Fc protein.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse Sp2/0 Ag.14 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P43121
- Entrez Gene
- MCAM
- GO Terms
- GO:0005886 plasma membrane
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0009653 anatomical structure morphogenesis
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD146 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | |||
ELISA | |||
Flow Cytometry | 1/50 | 1/100 | |
Immunofluorescence | |||
Immunohistology - Frozen | |||
Flow Cytometry | Neat | 1/10 | |
ELISA | |||
Flow Cytometry | 1/10 | 1/50 | |
Immunofluorescence | |||
Immunohistology - Frozen |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Melanoma
- Histology Positive Control Tissue
- Melanoma
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD146 antibody
-
Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
PLoS One. 7: e35577. -
Crisan, M. et al. (2008) A perivascular origin for mesenchymal stem cells in multiple human organs.
Cell Stem Cell. 3: 301-13. -
Iohara, K. et al. (2008) A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp.
Stem Cells. 26 (9): 2408-18. -
Park, T.S. et al. (2010) Placental Perivascular Cells for Human Muscle Regeneration.
Stem Cells Dev. 20: 451-63. -
Smith, K. et al. (2011) Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.
Immunology. 132: 256-65. -
James, A.W. et al. (2012) Perivascular stem cells: a prospectively purified mesenchymal stem cell population for bone tissue engineering.
Stem Cells Transl Med. 1 (6): 510-9. -
Wassmer, S.C. et al. (2011) Vascular endothelial cells cultured from patients with cerebral or uncomplicated malaria exhibit differential reactivity to TNF.
Cell Microbiol. 13: 198-209. -
Lee, J.H. et al. (2012) Generation of osteogenic construct using periosteal-derived osteoblasts and polydioxanone/pluronic F127 scaffold with periosteal-derived CD146 positive endothelial-like cells.
J Biomed Mater Res A.101: 942-53. -
Boneberg, E.M. et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process.
Microvasc Res. 78: 325-31. -
Murakami, M. et al. (2012) Identification of novel function of vimentin for quality standard for regenerated pulp tissue.
J Endod. 38: 920-6. -
Nielsen, C.T. et al. (2011) Distinct features of circulating microparticles and their relationship to clinical manifestations in systemic lupus erythematosus.
Arthritis Rheum. 63: 3067-77. -
Zhang, Y. et al. (2009) The Impact of Proliferative Potential of Umbilical Cord–Derived Endothelial Progenitor Cells and Hypoxia on Vascular Tubule Formation In Vitro.
Stem Cells Dev. 18: 359-75. -
Iversen, L.V. et al. (2013) A heparin-based method for flow cytometric analysis of microparticles directly from platelet-poor plasma in calcium containing buffer.
J Immunol Methods. 388 (1-2): 49-59. -
Meireles, A.L. et al. (2011) Increased levels of circulating endothelial progenitor cells in human T-cell lymphotropic virus type I carriers.
Arch Med Res. 42: 34-7. -
Iversen, L.V. et al. (2013) Circulating microparticles and plasma levels of soluble E- and P-selectins in patients with systemic sclerosis.
Scand J Rheumatol. 42 (6): 473-82. -
Murakami, M. et al. (2013) The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential.
Biomaterials. pii: S0142-9612(13)00942-3. -
Ruetze, M. et al. (2013) A novel niche for skin derived precursors in non-follicular skin.
J Dermatol Sci. 69: 132-9. -
Dokić, J. et al. (2013) Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells.
Eur J Immunol. 43: 1862-72. -
Chen, W.C. et al. (2015) Human myocardial pericytes: multipotent mesodermal precursors exhibiting cardiac specificity.
Stem Cells. 33 (2): 557-73. -
Iversen, L.V. et al. (2015) Cross-sectional study of soluble selectins, fractions of circulating microparticles and their relationship to lung and skin involvement in systemic sclerosis.
BMC Musculoskelet Disord. 16: 191. -
Tawonsawatruk T et al. (2016) Adipose derived pericytes rescue fractures from a failure of healing - non-union.
Sci Rep. 6: 22779. -
Boissier, R. et al. (2016) Histologic and urodynamic effects of autologous stromal vascular fraction extracted from fat tissue with minimal ex vivo manipulation on a porcine model of intrinsic sphincter deficiency
J Urology. Jun 2 [Epub ahead of print] -
Esteves, C.L. et al. (2017) Equine Mesenchymal Stromal Cells Retain a Pericyte-Like Phenotype.
Stem Cells Dev. 26 (13): 964-72. -
Stefanska, A. et al. (2016) Human kidney pericytes produce renin.
Kidney Int. 90 (6): 1251-61. -
Lapin, M. et al. (2016) MINDEC-An Enhanced Negative Depletion Strategy for Circulating Tumour Cell Enrichment.
Sci Rep. 6: 28929. -
Muerza-Cascante, M.L. et al. (2016) Endosteal-like extracellular matrix expression on melt electrospun written scaffolds.
Acta Biomater. pii: S1742-7061(16)30706-1. -
Eliasberg, C.D. et al. (2017) Perivascular Stem Cells Diminish Muscle Atrophy Following Massive Rotator Cuff Tears in a Small Animal Model.
J Bone Joint Surg Am. 99 (4): 331-41. -
James, A.W. et al. (2017) Isolation and characterization of canine perivascular stem/stromal cells for bone tissue engineering.
PLoS One. 12 (5): e0177308. -
Shen, J. et al. (2018) Effects of WNT3A and WNT16 on the Osteogenic and Adipogenic Differentiation of Perivascular Stem/Stromal Cells.
Tissue Eng Part A. 24 (1-2): 68-80. -
Lapin, M. et al. (2017) Single-cell mRNA profiling reveals transcriptional heterogeneity among pancreatic circulating tumour cells.
BMC Cancer. 17 (1): 390. -
Esteves, C.L. et al. (2017) Isolation and characterization of equine native MSC populations.
Stem Cell Res Ther. 8 (1): 80. -
Gaceb, A. et al. (2017) Pericytes secrete pro-regenerative molecules in response to platelet-derived growth factor-BB.
J Cereb Blood Flow Metab. : 271678X17719645. -
Noda, S. et al. (2019) Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation.
Sci Rep. 9 (1): 5430.
Further Reading
-
Kuzu, I. et al. (1993) Expression of adhesion molecules on the endothelium of normal tissue vessels and vascular tumors.
Lab Invest. 69 (3): 322-8. -
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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