CD142 antibody | TF9-10H10

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Mouse anti Human CD142

Product Type
Monoclonal Antibody
Clone
TF9-10H10
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
9010-5059 C F IF WB 0.1 mg
Mouse anti Human CD142 antibody, clone TF9-10H10 recognizes human CD142, also known as Tissue Factor, is the membrane receptor for coagulation factors VII and VIIa and is the cell surface initiator of coagulation. It is the major molecule of this type and is criticial for controlling hemostasis, thrombosis and inflammation.

Mouse anti Human CD142 antibody, clone TF9-10H10 recognizes an epitope within the extracellular domain, epitope locus I. It recognizes both the reduced and native non-reduced human and primate tissue factors. It does not inhibit coagulation or neutralize factor VII binding to CD142.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Primate
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
200 mMMannitol
Immunogen
Denatured Tissue factor isolated from human brain by the Factor VII affinity method (Guha et al. 1986).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the P3Ag8.653.1myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P13726 Related reagents
Entrez Gene
F3 Related reagents
GO Terms
GO:0001938 positive regulation of endothelial cell proliferation
GO:0002020 protease binding
GO:0002541 activation of plasma proteins involved in acute inflammatory response
GO:0002543 activation of blood coagulation via clotting cascade
GO:0016021 integral to membrane
GO:0005543 phospholipid binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006919 activation of caspase activity
GO:0007598 blood coagulation, extrinsic pathway
GO:0010641 positive regulation of platelet-derived growth factor receptor signaling pathway
GO:0031012 extracellular matrix
GO:0031233 intrinsic to external side of plasma membrane
GO:0043498 cell surface binding
GO:0045766 positive regulation of angiogenesis
GO:0050927 positive regulation of positive chemotaxis
GO:0051897 positive regulation of protein kinase B signaling cascade
Regulatory
For research purposes only

Applications of CD142 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Immunofluorescence

Western Blotting

Product Specific References

References for CD142 antibody

  1. Morrissey, J.H. et al. (1988) Monoclonal antibody analysis of purified and cell-associated tissue factor.
    Thromb Res. 52 (3): 247-61.
  2. Contrino, J. et al. (1994) In situ characterization of antigenic and functional tissue factor expression in human tumors utilizing monoclonal antibodies and recombinant factor VIIa as probes.
    Am J Pathol. 145 (6): 1315-22.
  3. Drake, T.A. et al. (1989) Selective cellular expression of tissue factor in human tissues. Implications for disorders of hemostasis and thrombosis.
    Am J Pathol. 134 (5): 1087-97.
  4. Stearns-Kurosawa, D.J. et al. (2006) Sepsis and pathophysiology of anthrax in a nonhuman primate model.
    Am J Pathol. 169 (2): 433-44.
  5. Mueller, B.M. et al. (1992) Expression of tissue factor by melanoma cells promotes efficient hematogenous metastasis.
    Proc Natl Acad Sci U S A. 89 (24): 11832-6.
  6. Agmon-Levin, N. et al. (2011) Vitamin D: an instrumental factor in the anti-phospholipid syndrome by inhibition of tissue factor expression.
    Ann Rheum Dis. 70: 145-50.
  7. Ruf, W. et al. (1991) Antibody mapping of tissue factor implicates two different exon-encoded regions in function.
    Biochem J. 278 ( Pt 3): 729-33.