CD142 antibody | TF9-10H10

Mouse anti Human CD142

Product Type
Monoclonal Antibody
Clone
TF9-10H10
Isotype
IgG1
Specificity
CD142

Product Code Applications Pack Size List Price Your Price Qty
9010-5059
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD142 antibody, clone TF9-10H10 recognizes human CD142, also known as Tissue Factor, is the membrane receptor for coagulation factors VII and VIIa and is the cell surface initiator of coagulation. It is the major molecule of this type and is criticial for controlling hemostasis, thrombosis and inflammation.

Mouse anti Human CD142 antibody, clone TF9-10H10 recognizes an epitope within the extracellular domain, epitope locus I. It recognizes both the reduced and native non-reduced human and primate tissue factors. It does not inhibit coagulation or neutralize factor VII binding to CD142.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Primate
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
100 mM Mannitol
Immunogen
Denatured Tissue factor isolated from human brain by the Factor VII affinity method (Guha et al. 1986).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the P3Ag8.653.1myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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Description Mouse IgG1 Negative Control

References for CD142 antibody

  1. Morrissey, J.H. et al. (1988) Monoclonal antibody analysis of purified and cell-associated tissue factor.
    Thromb Res. 52 (3): 247-61.
  2. Contrino, J. et al. (1994) In situ characterization of antigenic and functional tissue factor expression in human tumors utilizing monoclonal antibodies and recombinant factor VIIa as probes.
    Am J Pathol. 145 (6): 1315-22.
  3. Drake, T.A. et al. (1989) Selective cellular expression of tissue factor in human tissues. Implications for disorders of hemostasis and thrombosis.
    Am J Pathol. 134 (5): 1087-97.
  4. Stearns-Kurosawa, D.J. et al. (2006) Sepsis and pathophysiology of anthrax in a nonhuman primate model.
    Am J Pathol. 169 (2): 433-44.
  5. Mueller, B.M. et al. (1992) Expression of tissue factor by melanoma cells promotes efficient hematogenous metastasis.
    Proc Natl Acad Sci U S A. 89 (24): 11832-6.
  6. Agmon-Levin, N. et al. (2011) Vitamin D: an instrumental factor in the anti-phospholipid syndrome by inhibition of tissue factor expression.
    Ann Rheum Dis. 70: 145-50.
  7. Ruf, W. et al. (1991) Antibody mapping of tissue factor implicates two different exon-encoded regions in function.
    Biochem J. 278 ( Pt 3): 729-33.

Immunofluorescence

Western Blotting

Synonyms
Tissue Factor
RRID
AB_620060
UniProt
P13726
Entrez Gene
F3
GO Terms
GO:0001938 positive regulation of endothelial cell proliferation
GO:0002020 protease binding
GO:0002541 activation of plasma proteins involved in acute inflammatory response
GO:0002543 activation of blood coagulation via clotting cascade
GO:0016021 integral to membrane
GO:0005543 phospholipid binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006919 activation of caspase activity
GO:0007598 blood coagulation, extrinsic pathway
GO:0010641 positive regulation of platelet-derived growth factor receptor signaling pathway
GO:0031012 extracellular matrix
GO:0031233 intrinsic to external side of plasma membrane
GO:0043498 cell surface binding
GO:0045766 positive regulation of angiogenesis
GO:0050927 positive regulation of positive chemotaxis
GO:0051897 positive regulation of protein kinase B signaling cascade
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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