CD140a antibody | 35248 (57/20)

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Mouse anti Human CD140a

Product Type
Monoclonal Antibody
Clone
35248 (57/20)
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0100-0220 E IF P WB* 0.1 mg
Mouse anti Human CD140a antibody, clone 35248 recognizes the human platelet-derived growth factor receptor alpha, a 1089 amino acid, ~180 kDa single pass type I transmembrane glycoprotein.

PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulphide-linked PDGF-A and PDGF-B chains (Hart et al. 1990). The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF receptor alpha binds all three isoforms with high affinity, PDGF R beta binds PDGF-BB and AB but not PDGF-AA (Hart et al. 1988). Both PDGF receptor alpha and beta are members of the class III subfamily of receptor tyrosine kinases (Westermark 1995).

Mouse anti Human CD140a antibody, clone 35248 has been reported for use in functional studies. The ND50 was determined as 0.2-0.6 μg/ml for blocking 10 ng/ml of PDGF-AA biological activity on a quiescent confluent cell culture of human WS1 cells.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
5%Trehalose
Immunogen
Purified recombinant human platelet-derived growth factor (PDGF) receptor alpha (extracellular domain), derived from the insect cell line Sf21.
Approx. Protein Concentrations
1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P16234 Related reagents
Entrez Gene
PDGFRA Related reagents
GO Terms
GO:0001775 cell activation
GO:0005018 platelet-derived growth factor alpha-receptor activity
GO:0005021 vascular endothelial growth factor receptor activity
GO:0005887 integral to plasma membrane
GO:0005161 platelet-derived growth factor receptor binding
GO:0005524 ATP binding
GO:0018108 peptidyl-tyrosine phosphorylation
GO:0030335 positive regulation of cell migration
GO:0042803 protein homodimerization activity
GO:0045740 positive regulation of DNA replication
GO:0046777 protein autophosphorylation
GO:0048146 positive regulation of fibroblast proliferation
GO:0048407 platelet-derived growth factor binding
Regulatory
For research purposes only

Applications of CD140a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 µg/ml 1 µg/ml
Immunofluorescence
Immunohistology - Paraffin 10µg/ml 20µg/ml
Western Blotting 1 1 µg/ml 2 µg/ml
  1. 1This antibody recognizes CD140a under non-reducing conditions.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Product Specific References

References for CD140a antibody

  1. De Paula, M.L. et al. (2014) The PTEN inhibitor bisperoxovanadium enhances myelination by amplifying IGF-1 signaling in rat and human oligodendrocyte progenitors.
    Glia. 62 (1): 64-77.
  2. Preisner, A. et al. (2015) Non-steroidal anti-inflammatory drug indometacin enhances endogenous remyelination.
    Acta Neuropathol. 130 (2): 247-61.
  3. Kuroda, M. et al. (2017) Peripherally derived FGF21 promotes remyelination in the central nervous system.
    J Clin Invest. 127 (9): 3496-509.