CD14 antibody | MEM-18
Filter by Application:F E Reset
|Mouse anti Human CD14 antibody, clone MEM-18 recognizes human CD14, also known as Myeloid cell-specific leucine-rich glycoprotein. CD14 is a 375 amino acid ~55 kDa GPI-anchored cell membrane protein found predominantly on monocytes and macrophages, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology CD14 present on Langerhans cells, follicular dendritic cells, histocytes and high endothelial venules. In ELISA clone MEM-18 recognizes the soluble form CD14 and has been used successfully in the develpopment of a sensitive ELISA as a capture reagent in conjunction with biotinylated Mouse anti CD14 antibody, clone UCHM1 as a detection reagent
Mouse anti Human CD14 antibody, clone MEM-18 is reported to block the binding of bacterial lipopolysaccharide (LPS) to monocytes (Prager et al. 2001) and has been used successfully for the detection of soluble CD14 in saliva samples (Bergandi et al. 2007).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by caprylic acid and ammonium sulphate precipitation
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Crude protein prepared by ammonium sulfate precipitation of urine from a proteinuria patient.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from mice immunised BALB/c mice were fused with cells from the NS1-Ag4/1 mouse myeloma line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Western Blotting 1|
- 1 MEM-18 recognizes CD14 under non-reducing conditions
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD14 antibody
Plötz, S.G. et al. (2001) The interaction of human peripheral blood eosinophils with bacterial lipopolysaccharide is CD14 dependent.
Blood. 97 (1): 235-41.
Prager, E. et al. (2001) Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells.
J Immunol. 166 (4): 2364-71.
Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.
Vet Immunol Immunopathol. 80 (1-2): 93-109.
Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
PLoS One. 7: e35577.
Angel, C.E. et al. (2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.
J Immunol. 176: 5730-4.
Shao, D.D. et al. (2008) Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation.
J Leukoc Biol. 83: 1323-33.
Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
J Biomed Biotechnol. 2012: 172420.
Jafarshad, A. et al. (2007) A novel antibody-dependent cellular cytotoxicity mechanism involved in defense against malaria requires costimulation of monocytes FcgammaRII and FcgammaRIII.
J Immunol. 178: 3099-106.
View The Latest Product References
Kang, S.D. et al. (2013) Isolation of functional human endothelial cells from small volumes of umbilical cord blood.
Ann Biomed Eng. 41 (10): 2181-92.
Grognuz, A. et al. (2016) Human Fetal Progenitor Tenocytes for Regenerative Medicine.
Cell Transplant. 25 (3): 463-79.
Chen, R. et al. (2017) In Vitro Response of Human Peripheral Blood Mononuclear Cells (PBMC) to Collagen Films Treated with Cold Plasma.
Polymers (Basel). 9 (7): 254
- Entrez Gene
- GO Terms
- GO:0005886 plasma membrane
- GO:0001530 lipopolysaccharide binding
- GO:0001847 opsonin receptor activity
- GO:0006915 apoptosis
- GO:0006909 phagocytosis
- GO:0006954 inflammatory response
- GO:0008063 Toll signaling pathway
- GO:0031225 anchored to membrane
- GO:0016019 peptidoglycan receptor activity
- View More GO Terms
- GO:0032760 positive regulation of tumor necrosis factor production
- GO:0045087 innate immune response
- GO:0070891 lipoteichoic acid binding
- GO:0071222 cellular response to lipopolysaccharide
- GO:0071223 cellular response to lipoteichoic acid
Always be the first to know.
When we launch new products and resources to help you achieve more in the lab.Yes, sign me up