CD14 antibody | MEM-18

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Anti Human CD14 Antibody, clone MEM-18 thumbnail image 1

Anti Human CD14 Antibody, clone MEM-18 thumbnail image 2

Anti Human CD14 Antibody, clone MEM-18 thumbnail image 3

Anti Human CD14 Antibody, clone MEM-18 thumbnail image 4

Anti Human CD14 Antibody, clone MEM-18 thumbnail image 5

Anti Human CD14 Antibody, clone MEM-18 gallery image 1 — Staining of human peripheral blood monocytes with Mouse anti Human CD14:FITC (**MCA2185F**)

Anti Human CD14 Antibody, clone MEM-18 gallery image 2 — Staining of human peripheral blood monocytes with Mouse anti Human CD14:RPE (**MCA2185PE**)

Anti Human CD14 Antibody, clone MEM-18 gallery image 3 — Sandwich ELISA analysis of Human CD14 binding using Mouse anti CD14 antibody, clone MEM-18 (**MCA2185**) as a capture reagent and biotinylated Mouse anti Human CD14 antibody, clone UCHM1(MCA596GA) as a detection reagent with purified CD14 as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum samples diluted 1:200 and 1:400 are shown green and red respectively, while plasma samples also diluted 1:200 and 1:400 are blue and orange respectively

Anti Human CD14 Antibody, clone MEM-18 gallery image 4 — Sandwich ELISA analysis of Human CD14 binding using Mouse anti CD14 antibody, clone MEM-18 (**MCA2185**) as a capture reagent and biotinylated Mouse anti Human CD14 antibody, clone Tük4(MCA1568) as a detection reagent with purified CD14 as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum samples diluted 1:200 and 1:400 are shown green and red respectively, while plasma samples also diluted 1:200 and 1:400 are blue and orange respectively

Anti Human CD14 Antibody, clone MEM-18 gallery image 5 — **Published customer image:**
FITC conjugated Mouse anti Human CD14 antibody, clone MEM-18 (**MCA2185F**) used for the detection of monocytes in PBMCs by flow cytometry.
**Image caption:**
Accessory Cells Are Activated by iRBCs PBMCs from ten malaria-naïve donors were cultured with live iRBCs or E. coli LPS for 24 h or left unstimulated. Surface expression of HLA-DR, CD40, and CD80 on accessory cells was analysed by flow cytometry. Accessory cells were defined as HLA-DR+ CD11c+ cells (A, left), and CD14+ monocytes and CD14− mDCs were selected for further analysis (A, right). Representative examples of surface expression levels of CD40, HLA-DR, and CD80 on HLA-DR+ CD11c+ CD14+ monocytes (B) and HLA-DR+ CD11c+ CD14− mDCs (C) are shown. Data are based on the collection of 100,000 total events. Results for all ten donors are summarized in (D). *, p <0.05; **, p <0.01; ***, p <0.001; p-values are for paired t tests comparing stimulated and unstimulated cells from the same donor.

From: Newman KC, Korbel DS, Hafalla JC, Riley EM (2006)
Cross-Talk with Myeloid Accessory Cells Regulates Human Natural Killer Cell Interferon-γ Responses to Malaria.
PLoS Pathog 2(12): e118.

Mouse anti Human CD14:FITC

Mouse anti Human CD14

Mouse anti Human CD14:RPE

Product Type: Monoclonal Antibody
Clone: MEM-18
Isotype: IgG1

Product Code	Applications	Pack Size
MCA2185FT	F	25 µg
MCA2185F	F	0.1 mg
MCA2185	E F IP WB*	0.2 mg
MCA2185PET	F	25 Tests
MCA2185PE	F	100 Tests

Mouse anti Human CD14 antibody, clone MEM-18 recognizes human CD14, also known as Myeloid cell-specific leucine-rich glycoprotein. CD14 is a 375 amino acid ~55 kDa GPI-anchored cell membrane protein found predominantly on monocytes and macrophages, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology CD14 present on Langerhans cells, follicular dendritic cells, histocytes and high endothelial venules. In ELISA clone MEM-18 recognizes the soluble form CD14 and has been used successfully in the develpopment of a sensitive ELISA as a capture reagent in conjunction with biotinylated Mouse anti CD14 antibody, clone UCHM1 as a detection reagent

Mouse anti Human CD14 antibody, clone MEM-18 is reported to block the binding of bacterial lipopolysaccharide (LPS) to monocytes (Prager et al. 2001) and has been used successfully for the detection of soluble CD14 in saliva samples (Bergandi et al. 2007).

Product Details

Target Species

Human

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Pack Size: 25 Tests

Reconstitute in 0.25 ml disilled water

Pack Size: 100 Tests

Reconstitute with 1 ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by caprylic acid and ammonium sulphate precipitation

Preparation

Purified IgG prepared by caprylic acid and ammonium sulfate precipitation.

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose

Immunogen

Crude protein prepared by ammonium sulfate precipitation of urine from a proteinuria patient.

Approx. Protein Concentrations

IgG concentration 0.1mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from mice immunised BALB/c mice were fused with cells from the NS1-Ag4/1 mouse myeloma line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: P08571 Related reagents
Entrez Gene: CD14 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0001530 lipopolysaccharide binding; GO:0001847 opsonin receptor activity; GO:0006915 apoptosis; GO:0006909 phagocytosis; GO:0006954 inflammatory response; GO:0008063 Toll signaling pathway; GO:0031225 anchored to membrane; GO:0016019 peptidoglycan receptor activity; GO:0032760 positive regulation of tumor necrosis factor production; GO:0045087 innate immune response; GO:0070891 lipoteichoic acid binding; GO:0071222 cellular response to lipopolysaccharide; GO:0071223 cellular response to lipoteichoic acid
Regulatory: For research purposes only

Applications of CD14 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/10
ELISA
Flow Cytometry
Immunoprecipitation
Western Blotting ¹
Flow Cytometry		Neat

¹ MEM-18 recognizes CD14 under non-reducing conditions

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul or 100ul whole blood
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Application Based External Images

Flow Cytometry

Immunocytochemistry

Product Specific References

References for CD14 antibody

Plötz, S.G. et al. (2001) The interaction of human peripheral blood eosinophils with bacterial lipopolysaccharide is CD14 dependent.
Blood. 97 (1): 235-41.
Prager, E. et al. (2001) Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells.
J Immunol. 166 (4): 2364-71.
Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.
Vet Immunol Immunopathol. 80 (1-2): 93-109.
Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
PLoS One. 7: e35577.
Angel, C.E. et al. (2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.
J Immunol. 176: 5730-4.
Shao, D.D. et al. (2008) Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation.
J Leukoc Biol. 83: 1323-33.
Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
J Biomed Biotechnol. 2012: 172420.
Jafarshad, A. et al. (2007) A novel antibody-dependent cellular cytotoxicity mechanism involved in defense against malaria requires costimulation of monocytes FcgammaRII and FcgammaRIII.
J Immunol. 178: 3099-106.
Kang, S.D. et al. (2013) Isolation of functional human endothelial cells from small volumes of umbilical cord blood.
Ann Biomed Eng. 41 (10): 2181-92.
Grognuz, A. et al. (2016) Human Fetal Progenitor Tenocytes for Regenerative Medicine.
Cell Transplant. 25 (3): 463-79.
Chen, R. et al. (2017) In Vitro Response of Human Peripheral Blood Mononuclear Cells (PBMC) to Collagen Films Treated with Cold Plasma
Polymers. 9 (7): 254.

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