CD14 antibody | MEM-18

Mouse anti Human CD14

Product Type
Monoclonal Antibody
Clone
MEM-18
Isotype
IgG1
Specificity
CD14

Product Code Applications Pack Size List Price Your Price Qty
MCA2185
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F IP WB * 0.2 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD14 antibody, clone MEM-18 recognizes human CD14, also known as Myeloid cell-specific leucine-rich glycoprotein. CD14 is a 375 amino acid ~55 kDa GPI-anchored cell membrane protein found predominantly on monocytes and macrophages, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology CD14 present on Langerhans cells, follicular dendritic cells, histocytes and high endothelial venules. In ELISA clone MEM-18 recognizes the soluble form CD14 and has been used successfully in the develpopment of a sensitive ELISA as a capture reagent in conjunction with biotinylated Mouse anti CD14 antibody, clone UCHM1 as a detection reagent

Mouse anti Human CD14 antibody, clone MEM-18 is reported to block the binding of bacterial lipopolysaccharide (LPS) to monocytes (Prager et al. 2001) and has been used successfully for the detection of soluble CD14 in saliva samples (Bergandi et al. 2007).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by caprylic acid and ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Crude protein prepared by ammonium sulfate precipitation of urine from a proteinuria patient.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from mice immunised BALB/c mice were fused with cells from the NS1-Ag4/1 mouse myeloma line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Immunoprecipitation
Western Blotting 1
  1. 1 MEM-18 recognizes CD14 under non-reducing conditions
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

References for CD14 antibody

  1. Plötz, S.G. et al. (2001) The interaction of human peripheral blood eosinophils with bacterial lipopolysaccharide is CD14 dependent.
    Blood. 97 (1): 235-41.
  2. Prager, E. et al. (2001) Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells.
    J Immunol. 166 (4): 2364-71.
  3. Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.
    Vet Immunol Immunopathol. 80 (1-2): 93-109.
  4. Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
    PLoS One. 7: e35577.
  5. Angel, C.E. et al. (2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.
    J Immunol. 176: 5730-4.
  6. Shao, D.D. et al. (2008) Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation.
    J Leukoc Biol. 83: 1323-33.
  7. Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
    J Biomed Biotechnol. 2012: 172420.
  8. Jafarshad, A. et al. (2007) A novel antibody-dependent cellular cytotoxicity mechanism involved in defense against malaria requires costimulation of monocytes FcgammaRII and FcgammaRIII.
    J Immunol. 178: 3099-106.
  9. View The Latest Product References
  10. Kang, S.D. et al. (2013) Isolation of functional human endothelial cells from small volumes of umbilical cord blood.
    Ann Biomed Eng. 41 (10): 2181-92.
  11. Grognuz, A. et al. (2016) Human Fetal Progenitor Tenocytes for Regenerative Medicine.
    Cell Transplant. 25 (3): 463-79.
  12. Chen, R. et al. (2017) In Vitro Response of Human Peripheral Blood Mononuclear Cells (PBMC) to Collagen Films Treated with Cold Plasma.
    Polymers (Basel). 9 (7): 254

Flow Cytometry

Immunocytochemistry

RRID
AB_324230
UniProt
P08571
Entrez Gene
CD14
GO Terms
GO:0005886 plasma membrane
GO:0001530 lipopolysaccharide binding
GO:0001847 opsonin receptor activity
GO:0006915 apoptosis
GO:0006909 phagocytosis
GO:0006954 inflammatory response
GO:0008063 Toll signaling pathway
GO:0031225 anchored to membrane
GO:0016019 peptidoglycan receptor activity
GO:0032760 positive regulation of tumor necrosis factor production
GO:0045087 innate immune response
GO:0070891 lipoteichoic acid binding
GO:0071222 cellular response to lipopolysaccharide
GO:0071223 cellular response to lipoteichoic acid
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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