CD14 antibody | MEM-18
FITC conjugated Mouse anti Human CD14 antibody, clone MEM-18 (MCA2185F) used for the detection of monocytes in PBMCs by flow cytometry.
Image caption:
Accessory Cells Are Activated by iRBCs PBMCs from ten malaria-naïve donors were cultured with live iRBCs or E. coli LPS for 24 h or left unstimulated. Surface expression of HLA-DR, CD40, and CD80 on accessory cells was analysed by flow cytometry. Accessory cells were defined as HLA-DR+ CD11c+ cells (A, left), and CD14+ monocytes and CD14− mDCs were selected for further analysis (A, right). Representative examples of surface expression levels of CD40, HLA-DR, and CD80 on HLA-DR+ CD11c+ CD14+ monocytes (B) and HLA-DR+ CD11c+ CD14− mDCs (C) are shown. Data are based on the collection of 100,000 total events. Results for all ten donors are summarized in (D). *, p <0.05; **, p <0.01; ***, p <0.001; p-values are for paired t tests comparing stimulated and unstimulated cells from the same donor.
From: Newman KC, Korbel DS, Hafalla JC, Riley EM (2006)
Cross-Talk with Myeloid Accessory Cells Regulates Human Natural Killer Cell Interferon-γ Responses to Malaria.
PLoS Pathog 2(12): e118.
Mouse anti Human CD14 antibody, clone MEM-18 recognizes human CD14, also known as Myeloid cell-specific leucine-rich glycoprotein. CD14 is a 375 amino acid ~55 kDa GPI-anchored cell membrane protein found predominantly on monocytes and macrophages, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology CD14 present on Langerhans cells, follicular dendritic cells, histocytes and high endothelial venules. In ELISA clone MEM-18 recognizes the soluble form CD14 and has been used successfully in the develpopment of a sensitive ELISA as a capture reagent in conjunction with biotinylated Mouse anti CD14 antibody, clone UCHM1 as a detection reagent
Mouse anti Human CD14 antibody, clone MEM-18 is reported to block the binding of bacterial lipopolysaccharide (LPS) to monocytes (Prager et al. 2001) and has been used successfully for the detection of soluble CD14 in saliva samples (Bergandi et al. 2007).
Mouse anti Human CD14 antibody, clone MEM-18 is reported to block the binding of bacterial lipopolysaccharide (LPS) to monocytes (Prager et al. 2001) and has been used successfully for the detection of soluble CD14 in saliva samples (Bergandi et al. 2007).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by caprylic acid and ammonium sulphate precipitation
- Preparation
- Purified IgG prepared by caprylic acid and ammonium sulfate precipitation.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Immunogen
- Crude protein prepared by ammonium sulfate precipitation of urine from a proteinuria patient.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from mice immunised BALB/c mice were fused with cells from the NS1-Ag4/1 mouse myeloma line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of reconstitution.
More Information
- UniProt
- P08571 Related reagents
- Entrez Gene
- CD14 Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0001530 lipopolysaccharide binding
- GO:0001847 opsonin receptor activity
- GO:0006915 apoptosis
- GO:0006909 phagocytosis
- GO:0006954 inflammatory response
- GO:0008063 Toll signaling pathway
- GO:0031225 anchored to membrane
- GO:0016019 peptidoglycan receptor activity
- GO:0032760 positive regulation of tumor necrosis factor production
- GO:0045087 innate immune response
- GO:0070891 lipoteichoic acid binding
- GO:0071222 cellular response to lipopolysaccharide
- GO:0071223 cellular response to lipoteichoic acid
- Regulatory
- For research purposes only
Applications of CD14 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| ELISA | |||
| Flow Cytometry | |||
| Immunoprecipitation | |||
| Western Blotting 1 | |||
| Flow Cytometry | Neat |
- 1 MEM-18 recognizes CD14 under non-reducing conditions
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | 100 Tests | F | ||
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F | ||
| Mouse IgG1 Negative Control:RPE | MCA928PE | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F |
Product Specific References
References for CD14 antibody
-
Plötz, S.G. et al. (2001) The interaction of human peripheral blood eosinophils with bacterial lipopolysaccharide is CD14 dependent.
Blood. 97 (1): 235-41. -
Prager, E. et al. (2001) Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells.
J Immunol. 166 (4): 2364-71. -
Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.
Vet Immunol Immunopathol. 80 (1-2): 93-109. -
Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
PLoS One. 7: e35577. -
Angel, C.E. et al. (2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.
J Immunol. 176: 5730-4. -
Shao, D.D. et al. (2008) Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation.
J Leukoc Biol. 83: 1323-33. -
Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
J Biomed Biotechnol. 2012: 172420. -
Jafarshad, A. et al. (2007) A novel antibody-dependent cellular cytotoxicity mechanism involved in defense against malaria requires costimulation of monocytes FcgammaRII and FcgammaRIII.
J Immunol. 178: 3099-106. -
Kang, S.D. et al. (2013) Isolation of functional human endothelial cells from small volumes of umbilical cord blood.
Ann Biomed Eng. 41 (10): 2181-92. -
Grognuz, A. et al. (2016) Human Fetal Progenitor Tenocytes for Regenerative Medicine.
Cell Transplant. 25 (3): 463-79. -
Chen, R. et al. (2017) In Vitro Response of Human Peripheral Blood Mononuclear Cells (PBMC) to Collagen Films Treated with Cold Plasma
Polymers. 9 (7): 254.
