CD138 antibody | B-A38

Mouse anti Human CD138

Product Type
Monoclonal Antibody
Clone
B-A38
Isotype
IgG1
Specificity
CD138

Product Code Applications Pack Size List Price Your Price Qty
MCA2459GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F FN * IF P * WB 0.1 mg loader
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loader
MCA2459
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SDS Safety Datasheet SDS
C F FN * IF P * WB 2 ml loader
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loader
MCA2459T
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SDS Safety Datasheet SDS
C F IF P * WB 0.1 ml loader
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loader
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Mouse anti human CD138 antibody, clone B-A38 recognizes human CD138, also known as Syndecan-1 (SDC-1). CD138 is a member of the transmembrane heparan sulfate proteoglycan family (O’Connell et al. 2004, Sanderson et al. 2008). It is composed of a core protein (comprising 3 domains; a short cytoplasmic domain, a transmembrane domain, and a long extracellular domain) and covalently attached heparan sulfate chains (Sanderson et al. 2008).

Syndecan-1 is expressed on the surface of plasma cells within the hematopoietic system and on the surface of mature epithelial cells (O’Connell et al. 2004). It acts as an extracellular matrix receptor, involved in many cellular functions, including cell binding, cell signaling and cytoskeletal organization through cell-cell adhesion and cell-matrix adhesion (Sanderson et al. 2008).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
U266 cell line.
Approx. Protein Concentrations
Pack Size: 0.1 mg, 2 ml
IgG concentration 0.1 mg/ml
Pack Size: 0.1 ml
IgG concentration 0.1mg/ml
Fusion Partners
Spleen cells from immunized Balb/c (Iffa Credo) mice were fused with cells of the mouse X63/Ag.8653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Functional Assays 1
Immunofluorescence
Immunohistology - Frozen 1/100 1/500
Immunohistology - Paraffin 2 1/100 1/500 Pack Size: 0.1 mg, 0.1 ml
1/100 Pack Size: 2 ml
Western Blotting
  1. 1 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
  2. 2This product requires heat pre-treatment. Sodium citrate buffer pH6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Bone Marrow

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References for CD138 antibody

  1. Elbezanti, O.W. et al. (2022) Development of a Novel Bruton’s Tyrosine Kinase Inhibitor that exerts Anti-Cancer Activities Potentiates Response of Chemotherapeutic Agents In Multiple Myeloma Stem Cell-Like Cells
    bioRxiv 2022.03.10.483708 [Epub ahead of print].
  2. Borset, M. et al. (1993) Lack of IL-1 secretion from human myeloma cells highly purified by immunomagnetic separation.
    Br J Haematol. 85 (3): 446-51.
  3. Du, S. et al. (2010) Systemic mastocytosis in association with chronic lymphocytic leukemia and plasma cell myeloma.
    Int J Clin Exp Pathol. 3 (4): 448-57.
  4. Kylänpää, L. et al. (2009) Syndecan-1 and tenascin expression in cystic tumors of the pancreas.
    JOP. 10 (4): 378-82.
  5. Beauvais, D.M. et al. (2009) Syndecan-1 regulates alphavbeta3 and alphavbeta5 integrin activation during angiogenesis and is blocked by synstatin, a novel peptide inhibitor.
    J Exp Med. 206: 691-705.
  6. Beauvais, D.M. and Rapraeger, A.C. (2010) Syndecan-1 couples the insulin-like growth factor-1 receptor to inside-out integrin activation
    J Cell Sci. 123: 3796-807.
  7. Kim, Y.C. et al. (2010) Presence of Porphyromonas gingivalis and plasma cell dominance in gingival tissues with periodontitis.
    Oral Dis. 16: 375-81.
  8. Chang, H. et al. (2010) CKS1B nuclear expression is inversely correlated with p27Kip1 expression and is predictive of an adverse survival in patients with multiple myeloma.
    Haematologica. 95: 1542-7.
  9. View The Latest Product References
  10. Mahshid Y et al. (2009) High expression of 5-lipoxygenase in normal and malignant mantle zone B lymphocytes.
    BMC Immunol. 10: 2.
  11. Guedez, L. et al. (2005) Tissue inhibitor of metalloproteinase 1 (TIMP-1) promotes plasmablastic differentiation of a Burkitt lymphoma cell line: implications in the pathogenesis of plasmacytic/plasmablastic tumors.
    Blood. 105: 1660-8.
  12. Li, K. et al. (2010) Anaplastic lymphoma kinase-positive diffuse large B-cell lymphoma presenting as an isolated nasopharyngeal mass: a case report and review of literature.
    Int J Clin Exp Pathol. 4: 190-6.
  13. Yang, Y. et al. (2007) The syndecan-1 heparan sulfate proteoglycan is a viable target for myeloma therapy.
    Blood. 110: 2041-8.
  14. Thaunat, O. et al. (2010) Chronic rejection triggers the development of an aggressive intragraft immune response through recapitulation of lymphoid organogenesis.
    J Immunol. 185: 717-28.
  15. Cannizzo, E. et al. (2012) The role of CD19 and CD27 in the diagnosis of multiple myeloma by flow cytometry: a new statistical model.
    Am J Clin Pathol. 137 (3): 377-86.
  16. Li, K. et al. (2012) A rare and unique case of aggressive IgE-γ plasma cell myeloma in a 28-year-old woman presented initially as an orbital mass.
    Hum Pathol. 43: 2376-84.
  17. Christianson, H.C. et al. (2013) Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for their internalization and functional activity.
    Proc Natl Acad Sci U S A. 110 (43): 17380-5.
  18. Malminen, M. et al. (2002) Functional expression of NF1 tumor suppressor protein: association with keratin intermediate filaments during the early development of human epidermis.
    BMC Dermatol. 2: 10.
  19. Itoua Maïga, R. et al. (2014) Flow cytometry assessment of in vitro generated CD138+ human plasma cells.
    Biomed Res Int. 2014: 536482.
  20. Di Niro, R. et al. (2016) Responsive population dynamics and wide seeding into the duodenal lamina propria of transglutaminase-2-specific plasma cells in celiac disease.
    Mucosal Immunol. 9 (1): 254-64.
  21. Yigit, N. et al. (2015) Nuclear factor-erythroid 2, nerve growth factor receptor, and CD34-microvessel density are differentially expressed in primary myelofibrosis, polycythemia vera, and essential thrombocythemia.
    Hum Pathol. 46 (8): 1217-25.
  22. Lum, D. & Wong, K.P. (2006) Sarcomatoid plasmacytoma: a diagnosis not often considered.
    Pathology. 38 (6): 593-6.
  23. Gill, J. et al. (2009) A case of hyperIgG4 disease or IgG4-related sclerosing disease presenting as retroperitoneal fibrosis, chronic sclerosing sialadenitis and mediastinal lymphadenopathy.
    Pathology. 41 (3): 297-300.
  24. Adepu, S. et al. (2015) Incipient renal transplant dysfunction associates with tubular syndecan-1 expression and shedding.
    Am J Physiol Renal Physiol. 309 (2): F137-45.
  25. Hara, S. et al. (2016) Distribution and components of interstitial inflammation and fibrosis in IgG4-related kidney disease: analysis of autopsy specimens.
    Hum Pathol. 55: 164-73.
  26. Hosseini, A. et al. (2016) Morphometric analysis of inflammation in bronchial biopsies following exposure to inhaled diesel exhaust and allergen challenge in atopic subjects.
    Part Fibre Toxicol. 13: 2.
  27. Uenoyama, A. et al. (2016) Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.
    Biosci Biotechnol Biochem. 80 (7): 1344-55.
  28. Hourai, R. et al. (2017) IgG4-positive cell infiltration in various cardiovascular disorders - results from histopathological analysis of surgical samples.
    BMC Cardiovasc Disord. 17 (1): 52.
  29. Nagata, K. et al. (2017) Epstein-Barr Virus Lytic Reactivation Activates B Cells Polyclonally and Induces Activation-Induced Cytidine Deaminase Expression: A Mechanism Underlying Autoimmunity and Its Contribution to Graves' Disease.
    Viral Immunol. Mar 23. [Epub ahead of print]
  30. Tran, D.N. et al. (2017) Polychromatic flow cytometry is more sensitive than microscopy in detecting small monoclonal plasma cell populations.
    Cytometry B Clin Cytom. 92 (2): 136-144.
  31. Puchalapalli, M. et al. (2019) The Laminin-α1 Chain-Derived Peptide, AG73, Binds to Syndecans on MDA-231 Breast Cancer Cells and Alters Filopodium Formation.
    Anal Cell Pathol (Amst). 2019: 9192516.
  32. Mittal, S. et al. (2013) Lymphoid aggregates that resemble tertiary lymphoid organs define a specific pathological subset in metal-on-metal hip replacements.
    PLoS One. 8 (5): e63470.
  33. Egeland, N.G. et al. (2020) MiR-18a and miR-18b are expressed in the stroma of oestrogen receptor alpha negative breast cancers.
    BMC Cancer. 20 (1): 377.
  34. Forsberg, P.A. et al. (2019) Cellular proliferation by multiplex immunohistochemistry identifies aggressive disease behavior in relapsed multiple myeloma.
    Leuk Lymphoma. 60 (8): 2085-7.
  35. Ebian, F.H. et al. (2021) Predictive Value of CD229, CD319 and c-Maf Overexpression for Treatment Response in Multiple Myeloma Patients
    Egypt J Hosp Med. 85 (1): 3157-65.
  36. Hara, S. et al. (2019) High Level Estradiol Induces EBV Reactivation and EBV gp350/220(+)CD138(+) Double-positive B Cell Population in Graves' Disease Patients and Healthy Controls.
    Yonago Acta Med. 62 (2): 240-243.

Further Reading

  1. Anttonen, A. et al. (1999) Syndecan-1 expression has prognostic significance in head and neck carcinoma.
    Br J Cancer. 79 (3-4): 558-64.
  2. O'Connell, F.P. et al. (2004) CD138 (syndecan-1), a plasma cell marker immunohistochemical profile in hematopoietic and nonhematopoietic neoplasms.
    Am J Clin Pathol. 121:254-63.
  3. Sanderson, R.D. et al. (2008) Syndecan-1: a dynamic regulator of the myeloma microenvironment.
    Clin Exp Metastasis. 25:149-59.

Functional Assays

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

Synonyms
SYNDECAN-1
RRID
AB_566507
UniProt
P18827
Entrez Gene
SDC1
GO Terms
GO:0005887 integral to plasma membrane
GO:0005737 cytoplasm
GO:0005925 focal adhesion
GO:0006629 lipid metabolic process
GO:0008022 protein C-terminus binding
GO:0008092 cytoskeletal protein binding
GO:0042157 lipoprotein metabolic process
GO:0048627 myoblast development
GO:0055002 striated muscle cell development
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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