CD138 antibody | B-A38











Mouse anti Human CD138:Alexa Fluor® 647
Mouse anti Human CD138:FITC
Mouse anti Human CD138
Mouse anti Human CD138:RPE
- Product Type
- Monoclonal Antibody
- Clone
- B-A38
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA2459A647T | F | 25 Tests/0.25ml |
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MCA2459A647 | F | 100 Tests/1ml |
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MCA2459FT | F | 25 µg |
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MCA2459F | F | 0.1 mg |
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MCA2459T | C F IF P* WB | 0.1 ml |
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MCA2459GA | C F FN* IF P* WB | 0.1 mg |
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MCA2459 | C F FN* IF P* WB | 2 ml |
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MCA2459PE | F | 100 Tests |
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MCA2459PET | F | 25 Tests |
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Syndecan-1 is expressed on the surface of plasma cells within the hematopoietic system and on the surface of mature epithelial cells (O’Connell et al. 2004). It acts as an extracellular matrix receptor, involved in many cellular functions, including cell binding, cell signaling and cytoskeletal organization through cell-cell adhesion and cell-matrix adhesion (Sanderson et al. 2008).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilizedPack Size: 25 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
- Reconstitution
- Pack Size: 100 TestsReconstitute with 1 ml distilled waterPack Size: 25 TestsReconstitute with 0.25 ml distilled water
- Preparation
- Purified IgG prepared by ion exchange chromatography.
- Preparation
- Purified IgG prepared by ion exchange chromatography
- Preparation
- Purified IgG prepared by ion exchange chromatography
- Preparation
- Purified IgG prepared by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Immunogen
- U266 cell line.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- Pack Size: 0.1 mlIgG concentration 0.1mg/mlPack Size: 0.1 mg, 2 mlIgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c (Iffa Credo) mice were fused with cells of the mouse X63/Ag.8653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 100 TestsPrior to reconstitution store at +4oC following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 25 TestsPrior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P18827
- Entrez Gene
- SDC1
- GO Terms
- GO:0005887 integral to plasma membrane
- GO:0005737 cytoplasm
- GO:0005925 focal adhesion
- GO:0006629 lipid metabolic process
- GO:0008022 protein C-terminus binding
- GO:0008092 cytoskeletal protein binding
- GO:0042157 lipoprotein metabolic process
- GO:0048627 myoblast development
- GO:0055002 striated muscle cell development
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD138 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | ||
Flow Cytometry | Neat | ||
Flow Cytometry | Neat | ||
Functional Assays 1 | |||
Immunofluorescence | |||
Immunohistology - Frozen | 1/100 | 1/500 | |
Immunohistology - Paraffin 2 | 1/100 | 1/500 Pack Size: 0.1 ml, 0.1 mg 1/100 Pack Size: 2 ml |
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Western Blotting | |||
Flow Cytometry | Neat | 1/5 |
- 1 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
- 2This product requires heat pre-treatment. Sodium citrate buffer pH6.0 is recommended for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Bone Marrow
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
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Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Application Based External Images
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Product Specific References
References for CD138 antibody
-
Borset, M. et al. (1993) Lack of IL-1 secretion from human myeloma cells highly purified by immunomagnetic separation.
Br J Haematol. 85 (3): 446-51. -
Du, S. et al. (2010) Systemic mastocytosis in association with chronic lymphocytic leukemia and plasma cell myeloma.
Int J Clin Exp Pathol. 3 (4): 448-57. -
Kylänpää, L. et al. (2009) Syndecan-1 and tenascin expression in cystic tumors of the pancreas.
JOP. 10 (4): 378-82. -
Beauvais, D.M. et al. (2009) Syndecan-1 regulates alphavbeta3 and alphavbeta5 integrin activation during angiogenesis and is blocked by synstatin, a novel peptide inhibitor.
J Exp Med. 206: 691-705. -
Beauvais, D.M. and Rapraeger, A.C. (2010) Syndecan-1 couples the insulin-like growth factor-1 receptor to inside-out integrin activation
J Cell Sci. 123: 3796-807. -
Kim, Y.C. et al. (2010) Presence of Porphyromonas gingivalis and plasma cell dominance in gingival tissues with periodontitis.
Oral Dis. 16: 375-81. -
Chang, H. et al. (2010) CKS1B nuclear expression is inversely correlated with p27Kip1 expression and is predictive of an adverse survival in patients with multiple myeloma.
Haematologica. 95: 1542-7. -
Mahshid Y et al. (2009) High expression of 5-lipoxygenase in normal and malignant mantle zone B lymphocytes.
BMC Immunol. 10: 2. -
Guedez, L. et al. (2005) Tissue inhibitor of metalloproteinase 1 (TIMP-1) promotes plasmablastic differentiation of a Burkitt lymphoma cell line: implications in the pathogenesis of plasmacytic/plasmablastic tumors.
Blood. 105: 1660-8. -
Li, K. et al. (2010) Anaplastic lymphoma kinase-positive diffuse large B-cell lymphoma presenting as an isolated nasopharyngeal mass: a case report and review of literature.
Int J Clin Exp Pathol. 4: 190-6. -
Yang, Y. et al. (2007) The syndecan-1 heparan sulfate proteoglycan is a viable target for myeloma therapy.
Blood. 110: 2041-8. -
Thaunat, O. et al. (2010) Chronic rejection triggers the development of an aggressive intragraft immune response through recapitulation of lymphoid organogenesis.
J Immunol. 185: 717-28. -
Cannizzo, E. et al. (2012) The role of CD19 and CD27 in the diagnosis of multiple myeloma by flow cytometry: a new statistical model.
Am J Clin Pathol. 137 (3): 377-86. -
Li, K. et al. (2012) A rare and unique case of aggressive IgE-γ plasma cell myeloma in a 28-year-old woman presented initially as an orbital mass.
Hum Pathol. 43: 2376-84. -
Christianson, H.C. et al. (2013) Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for their internalization and functional activity.
Proc Natl Acad Sci U S A. 110 (43): 17380-5. -
Malminen, M. et al. (2002) Functional expression of NF1 tumor suppressor protein: association with keratin intermediate filaments during the early development of human epidermis.
BMC Dermatol. 2: 10. -
Itoua Maïga, R. et al. (2014) Flow cytometry assessment of in vitro generated CD138+ human plasma cells.
Biomed Res Int. 2014: 536482. -
Di Niro, R. et al. (2016) Responsive population dynamics and wide seeding into the duodenal lamina propria of transglutaminase-2-specific plasma cells in celiac disease.
Mucosal Immunol. 9 (1): 254-64. -
Yigit, N. et al. (2015) Nuclear factor-erythroid 2, nerve growth factor receptor, and CD34-microvessel density are differentially expressed in primary myelofibrosis, polycythemia vera, and essential thrombocythemia.
Hum Pathol. 46 (8): 1217-25. -
Lum, D. & Wong, K.P. (2006) Sarcomatoid plasmacytoma: a diagnosis not often considered.
Pathology. 38 (6): 593-6. -
Gill, J. et al. (2009) A case of hyperIgG4 disease or IgG4-related sclerosing disease presenting as retroperitoneal fibrosis, chronic sclerosing sialadenitis and mediastinal lymphadenopathy.
Pathology. 41 (3): 297-300. -
Adepu, S. et al. (2015) Incipient renal transplant dysfunction associates with tubular syndecan-1 expression and shedding.
Am J Physiol Renal Physiol. 309 (2): F137-45. -
Hara, S. et al. (2016) Distribution and components of interstitial inflammation and fibrosis in IgG4-related kidney disease: Analysis of autopsy specimens
Hum Pathol. May 28 [Epub ahead of print] -
Hosseini, A. et al. (2016) Morphometric analysis of inflammation in bronchial biopsies following exposure to inhaled diesel exhaust and allergen challenge in atopic subjects.
Part Fibre Toxicol. 13: 2. -
Uenoyama, A. et al. (2016) Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.
Biosci Biotechnol Biochem. 80 (7): 1344-55. -
Hourai, R. et al. (2017) IgG4-positive cell infiltration in various cardiovascular disorders - results from histopathological analysis of surgical samples.
BMC Cardiovasc Disord. 17 (1): 52. -
Nagata, K. et al. (2017) Epstein-Barr Virus Lytic Reactivation Activates B Cells Polyclonally and Induces Activation-Induced Cytidine Deaminase Expression: A Mechanism Underlying Autoimmunity and Its Contribution to Graves' Disease.
Viral Immunol. Mar 23. [Epub ahead of print] -
Tran, D.N. et al. (2017) Polychromatic flow cytometry is more sensitive than microscopy in detecting small monoclonal plasma cell populations.
Cytometry B Clin Cytom. 92 (2): 136-144. -
Puchalapalli, M. et al. (2019) The Laminin-α1 Chain-Derived Peptide, AG73, Binds to Syndecans on MDA-231 Breast Cancer Cells and Alters Filopodium Formation.
Anal Cell Pathol (Amst). 2019: 9192516. -
Mittal, S. et al. (2013) Lymphoid aggregates that resemble tertiary lymphoid organs define a specific pathological subset in metal-on-metal hip replacements.
PLoS One. 8 (5): e63470.
Further Reading
-
Anttonen, A. et al. (1999) Syndecan-1 expression has prognostic significance in head and neck carcinoma.
Br J Cancer. 79 (3-4): 558-64. -
O'Connell, F.P. et al. (2004) CD138 (syndecan-1), a plasma cell marker immunohistochemical profile in hematopoietic and nonhematopoietic neoplasms.
Am J Clin Pathol. 121:254-63. -
Sanderson, R.D. et al. (2008) Syndecan-1: a dynamic regulator of the myeloma microenvironment.
Clin Exp Metastasis. 25:149-59.
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