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CD138 antibody | B-A38

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Anti Human CD138 Antibody, clone B-A38 thumbnail image 1 Anti Human CD138 Antibody, clone B-A38 thumbnail image 2 Anti Human CD138 Antibody, clone B-A38 thumbnail image 3 Anti Human CD138 Antibody, clone B-A38 thumbnail image 4 Anti Human CD138 Antibody, clone B-A38 thumbnail image 5 Anti Human CD138 Antibody, clone B-A38 thumbnail image 6 Anti Human CD138 Antibody, clone B-A38 thumbnail image 7 Anti Human CD138 Antibody, clone B-A38 thumbnail image 8 Anti Human CD138 Antibody, clone B-A38 thumbnail image 9 Anti Human CD138 Antibody, clone B-A38 thumbnail image 10 Anti Human CD138 Antibody, clone B-A38 thumbnail image 11 Anti Human CD138 Antibody, clone B-A38 thumbnail image 12
Anti Human CD138 Antibody, clone B-A38 gallery image 1
Staining of U266 cell line with Alexa Fluor® 647 conjugated Mouse anti Human CD138 antibody, clone B-A38 (MCA2459A647)
Anti Human CD138 Antibody, clone B-A38 gallery image 2
Staining of U266 cell line with FITC conjugated Mouse anti Human CD138 antibody, clone B-A38 (MCA2459F)
Anti Human CD138 Antibody, clone B-A38 gallery image 3
Published customer image:
Mouse anti Human CD138 antibody, clone B-A38 (MCA2459GA) used for the detection of syndecan-1 expressing cells in human skin by immunofluorescence.
Image caption:
Double immunolabeling and CLSM analysis of developing and adult epidermis for NF1 protein and syndecan-1. (a) At 14 weeks EGA, the NF1 protein (red) shows fibrillar pattern in basal cells while syndecan-1 (green) is localized to the plasma membrane of keratinocytes in all epidermal layers. Close spatial distribution or colocalization (yellow) is detected on some basal cells. (b) In adult epidermis the immunosignals for the NF1 protein (red) and syndecan-1 (green) are completely separate. Scale bars 10 μm in a,b, 2 μm in c,d.

From: Malminen M, Peltonen S, Koivunen J, Peltonen J.
Functional expression of NF1 tumor suppressor protein: association with keratin intermediate filaments during the early development of human epidermis.
BMC Dermatol. 2002 Aug 29;2:10.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD138 Antibody, clone B-A38 gallery image 4
Published customer image:
AlexaFluor A647 conjugated Mouse anti Human CD138 antibody, clone B-A38 (MCA2459A647) used for the detection of CD138 on bone marrow cells by flow cytometry.
Image caption:
B-A38 and DL-101 are both present on bone marrow plasma cells. Four bone marrow samples were used to test the capacity of DL-101 to detect CD138+ cells. (a) The analysis was done on viable cells, using Sytox Blue exclusion, and then on gated cells, which represented less than 0.5% of total cells. (b) Profiles are showed for B-A38 and DL-101 PE-conjugated mAbs for one sample gated on region. The mean frequency ± S.E.M for CD138+ cells was 78.7% ± 1.2% for B-A38 and 59.3% ± 2.1% for DL-101. (c) Frequencies of total are shown for all bone marrow samples according to B-A38 and DL-101; the mean ± S.E.M. was 0.20% ± 0.06% and 0.11% ± 0.05%, respectively. No significant differences were observed between the B-A38 and DL-101 staining in these four bone marrow samples (nonparametric Kruskal-Wallis test).

From: Rayelle Itoua Maïga, Jennifer Lemieux, Annie Roy, Carl Simard, and Sonia Néron,
“Flow Cytometry Assessment of In Vitro Generated CD138+ Human Plasma Cells,”
BioMed Research International, vol. 2014, Article ID 536482, 8 pages, 2014.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD138 Antibody, clone B-A38 gallery image 5
Published customer image:
Mouse anti Human CD138 antibody, clone B-A38 (MCA2459GA) used for the detection of syndecan-1 expressing cells in lymphoid follicles by immunofluorescence.
Image caption:
Expression of the B cell survival factors BAFF and April in ectopic lymphoid follicles and the presence of plasma cells in ectopic lymphoid follicles. Immunofluorescence images of staining of BAFF (red) and April (green) detected in the CD21+ follicular dendritic cell network (blue) (A) Paraffin sections of tissues showing organised lymphoid aggregates were also stained with a marker for plasma cells, CD138 (cyan), and B cells (CD20) (red) by double immunofluorescence (B). Abundant plasma cells were detected at the periphery of the aggregates as well as in the surrounding tissue. Shown are representative images from the group of cases containing organised lymphoid aggregates.

From: Mittal S, Revell M, Barone F, Hardie DL, Matharu GS, et al. (2013)
Lymphoid Aggregates That Resemble Tertiary Lymphoid Organs Define a Specific Pathological Subset in Metal-on-Metal Hip Replacements.
PLoS ONE 8(5): e63470.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD138 Antibody, clone B-A38 gallery image 6
Published customer image:
Mouse anti Human CD138 antibody, clone B-A38 (MCA2459GA) used for the detection of syndecan-1 expressing cells in human colonic tissues by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Expression of syndecan-1 in normal colonic epithelium and colon adenocarcinomas. a, immunohistochemical staining for syndecan-1 in normal colon. b-c, syndecan-1 staining of colon adenocarcinomas is lost in correlation with tumor differentiation status. d, example of a moderately differentiated adenocarcinoma with strong stromal staining for syndecan-1 and moderate staining of the tumor. e, f, contiguous sections of the same tumor stained for syndecan-1, (e), or fascin, (f), demonstrating strong staining for fascin in the stroma (arrowed). The tumor cells are negative for fascin and syndecan-1. Isolated strongly fascin positive cells correspond to dendritic and vascular endothelial cells and provideinternal positive controls for the fascin staining.

From: Hashimoto Y, Skacel M, Adams JC.
Association of loss of epithelial syndecan-1 with stage and local metastasis of colorectal adenocarcinomas: an immunohistochemical study of clinically annotated tumors.
BMC Cancer. 2008 Jun 30;8:185.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD138 Antibody, clone B-A38 gallery image 7
Published customer image:
Mouse anti Human CD138, clone B-A38 (MCA2459GA) used for the identification of plasma cells in the bronchial submucosa by immunohistochemistry on acetone fixed, resin embedded tissue sections.
Image caption:
Immunohistochemical staining of CD138-positive plasma cells in human bronchial submucosa tissue. a Representative 20X image of positive staining using mAb B-A38 for CD138 with positive staining in red with Mayer's hematoxylin counterstain in blue from a subject exposed to FAA. b Zoom region (40X) highlighted in black box of panel a. c Positive pixel count quantification of submucosa region for CD138 staining. Data are expressed as mean ± SEM. n = 9 for each experimental condition. *p < 0.05; **p < 0.01

From: Hosseini A, Hirota JA, Hackett TL, McNagny KM, Wilson SJ, Carlsten C.
Morphometric analysis of inflammation in bronchial biopsies following exposure to inhaled diesel exhaust and allergen challenge in atopic subjects.
Part Fibre Toxicol. 2016 Jan 13;13:2.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD138 Antibody, clone B-A38 gallery image 8
Published customer image:
Mouse anti Human CD138 antibody, clone B-A38 (MCA2459GA) used for the evaluation of syndecan-1 expression on plasma cells in cardiovascular surgical specimens by immunohistochemistry on formalin fixed paraffin embedded tissue sections.
Image caption:
Histopathological findings of tissue samples obtained from two patients with aortic stenosis (case 6 and case 8). a-d Case 6. e-h Case 8. a, b, e, f Hematoxylin and eosin (HE) staining. c, g IgG4 staining. d, h CD138 staining. b Higher magnification image of the bracketed area in a. The number of IgG4-positive cells was 73/HPF and the IgG4/CD138 ratio was 45%. f Higher magnification image of the bracketed area in e. Original magnification, ×40 (a, e), and × 200 (b-d, f-h). The number of IgG4-positive cells was 20/HPF and IgG4/CD138 ratio was 17%

From: Hourai R, Kasashima S, Sohmiya K, Yamauchi Y, Ozawa H, Hirose Y, Ogino Y, Katsumata T, Daimon M, Fujita SI, Hoshiga M, Ishizaka N.
IgG4-positive cell infiltration in various cardiovascular disorders - results from histopathological analysis of surgical samples.<
BMC Cardiovasc Disord. 2017 Feb 3;17(1):52.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD138 Antibody, clone B-A38 gallery image 9
Published customer image:
Mouse anti Human CD138 antibody, clone B-A38 (MCA2459GA) used for the determination of CD138 in MDA-231 breast cancer cells (a) by flow cytometry demonstrating the loss of surface expression following CD138 RNA knockown.
Image caption:
Expression of Sdcs 1, 2, and 4 is decreased by 75% at the protein (a, b) and message (c) levels in MDA-231 cells. (a, b) Flow cytometry measuring surface expression of Sdcs in MDA-231: (a) only Sdc 1 expression is decreased at the cell surface when the Sdc 1 expression is silenced in the MDA-231 cells. (b) In Sdc 2 KD cells, only the Sdc 2 surface expression is decreased, and in Sdc 4 KD cells, only the Sdc 4 surface expression is decreased. The decreased expression of these Sdcs is specific. (c) qPCR was used to detect the message levels of each Sdc. The fold change is plotted in comparison to the control. (d) Solid-phase assays were performed with the Sdc KD cells as described in Figure 2(b). Sdcs 1 and 4 bind to AG73 in control cells (NS1 and NS2) as well as the Sdc 2 KD cell lysate. No binding to AG73 was detected in the Sdc 1 and 4 KD cell lysate. Silencing of Sdcs 1 or 4 expression inhibited binding of these receptors to AG73, and thus, binding of the Sdc 1 and 4 antibodies was significantly decreased. Bars: mean OD 450 nm ± SEM. MDA-231-Sdc 1 KD cells compared to MDA-231-NS1 cells. MDA-231-Sdc 2 and 4 KD cells compared to MDA-231-NS2 cells. ∗∗∗ p <0.001. One-way ANOVA with Bonferroni posttest.

From: Puchalapalli M, Mu L, Edwards C, Kaplan-Singer B, Eni P, Belani K, Finkelstein D, Patel A, Sayyad M, Koblinski JE.
The Laminin-α1 Chain-Derived Peptide, AG73, Binds to Syndecans on MDA-231 Breast Cancer Cells and Alters Filopodium Formation.
Anal Cell Pathol (Amst). 2019 Apr 30;2019:9192516.
doi: 10.1155/2019/9192516.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD138 Antibody, clone B-A38 gallery image 10
Published customer image:
Mouse anti human CD138 antibody, clone B-A38 (MCA2459GA) used for the identification of plasma cells in human tonsillar cryostat sections by immunofluorescence.
Image caption:
Immunofluorescence analysis of 5-LO expression in subsets of tonsillar B lymphocytes. Tonsils were prepared for immunofluorescence analyses and stained with anti-IgD, anti-CD38, anti-CD138 and DAPI. A) B cells within the mantle zone were stained with anti-IgD (green, A1); anti-5-LO (red, A2) and DAPI (blue, A3). The combined picture (A4 (A1+A2+A3)) depicts high expression of 5-LO in IgD+ cells. B) Germinal centre B cells were stained with anti-CD38 (green, B1); anti-5-LO (red, B2) and DAPI (blue, B3). The combined picture (B4 (B1+B2+B3)) shows very weak expression of 5-LO in CD38++ cells. C) Plasma cells within the germinal centre were stained with anti-CD38 (green, C1); anti-5-LO (red, C2) and DAPI (blue, C3). The combined picture (C4 (C1+C2+C3)) demonstrates no expression of 5-LO in CD38+++ cells. D) Plasma cells outside the germinal centre were stained with anti-CD138 (green, D1); anti-5-LO (red, D2) and DAPI (blue, D3). The combined picture (D4 (D1+D2+D3)) depicts no expression of 5-LO in CD138+ cells. The figure represents one out of four examined tonsils.

From: Mahshid Y, Lisy MR, Wang X, Spanbroek R, Flygare J, Christensson B, Björkholm M, Sander B, Habenicht AJ, Claesson HE.
High expression of 5-lipoxygenase in normal and malignant mantle zone B lymphocytes.
BMC Immunol. 2009 Jan 9;10:2.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD138 Antibody, clone B-A38 gallery image 11
Published customer image:
Mouse anti human CD138 antibody, clone B-A38 (MCA2459GA) used to visualize plasma cells in human lymphoid follicles by immunofluorescence.
Image caption:
Expression of the B cell survival factors BAFF and April in ectopic lymphoid follicles and the presence of plasma cells in ectopic lymphoid follicles.
Immunofluorescence images of staining of BAFF (red) and April (green) detected in the CD21+ follicular dendritic cell network (blue) (A) Paraffin sections of tissues showing organised lymphoid aggregates were also stained with a marker for plasma cells, CD138 (cyan), and B cells (CD20) (red) by double immunofluorescence (B). Abundant plasma cells were detected at the periphery of the aggregates as well as in the surrounding tissue. Shown are representative images from the group of cases containing organised lymphoid aggregates.

From: Mittal S, Revell M, Barone F, Hardie DL, Matharu GS, Davenport AJ, et al. (2013)
Lymphoid Aggregates That Resemble Tertiary Lymphoid Organs Define a Specific Pathological Subset in Metal-on-Metal Hip Replacements.
PLoS ONE 8(5): e63470.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human CD138 Antibody, clone B-A38 gallery image 12
Published customer image:
Mouse anti human CD138 antibody, clone B-A38 (MCA2459) used to illustrate syndecan-1 expression in Human ER- breast cancer samples by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Expression pattern in an ER- breast tumour (sample id: 13 ER- in Table 2) of a) CISH LNA 5'-3’DIG hsa (80 nM) miR-18a probe and b) CISH LNA 5'-3’DIG hsa miR-18b (80 nM) probe in comparison to IHC staining for c) ER, d) CD4, e) CD8, f) CD20, g) CD68, h) CD138, i) PAX5 and j) actin. Scale bar 100 μm

From: Egeland NG, Jonsdottir K, Aure MR, Sahlberg K, Kristensen VN, Cronin-Fenton D, Skaland I, Gudlaugsson E, Baak JPA, Janssen EAM.
MiR-18a and miR-18b are expressed in the stroma of oestrogen receptor alpha negative breast cancers.
BMC Cancer. 2020 May 5;20(1):377.
doi: 10.1186/s12885-020-06857-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Human CD138:Alexa Fluor® 647

Mouse anti Human CD138:FITC

Mouse anti Human CD138

Mouse anti Human CD138:RPE

Product Type
Monoclonal Antibody
Clone
B-A38
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2459A647
Datasheet
F
SDS
100 Tests/1ml loader

MCA2459A647T
Datasheet
F
SDS
25 Tests/0.25ml loader

MCA2459FT
Datasheet
F
SDS
25 µg loader

MCA2459F
Datasheet
F
SDS
0.1 mg loader

MCA2459
Datasheet
C F FN* IF P* WB
SDS
2 ml loader

MCA2459GA
Datasheet
C F FN* IF P* WB
SDS
0.1 mg loader

MCA2459T
Datasheet
C F IF P* WB
SDS
0.1 ml loader

MCA2459PE
Datasheet
F
SDS
100 Tests loader

MCA2459PET
Datasheet
F
SDS
25 Tests loader

Mouse anti human CD138 antibody, clone B-A38 recognizes human CD138, also known as Syndecan-1 (SDC-1). CD138 is a member of the transmembrane heparan sulfate proteoglycan family (O’Connell et al. 2004, Sanderson et al. 2008). It is composed of a core protein (comprising 3 domains; a short cytoplasmic domain, a transmembrane domain, and a long extracellular domain) and covalently attached heparan sulfate chains (Sanderson et al. 2008).

Syndecan-1 is expressed on the surface of plasma cells within the hematopoietic system and on the surface of mature epithelial cells (O’Connell et al. 2004). It acts as an extracellular matrix receptor, involved in many cellular functions, including cell binding, cell signaling and cytoskeletal organization through cell-cell adhesion and cell-matrix adhesion (Sanderson et al. 2008).

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Pack Size: 100 Tests
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Pack Size: 25 Tests
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Reconstitution
Pack Size: 100 Tests
Reconstitute with 1 ml distilled water
Pack Size: 25 Tests
Reconstitute with 0.25 ml distilled water
Preparation
Purified IgG prepared by ion exchange chromatography.
Preparation
Purified IgG prepared by ion exchange chromatography
Preparation
Purified IgG prepared by ion exchange chromatography
Preparation
Purified IgG prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
U266 cell line.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
Pack Size: 2 ml, 0.1 mg
IgG concentration 0.1 mg/ml
Pack Size: 0.1 ml
IgG concentration 0.1mg/ml
Fusion Partners
Spleen cells from immunized Balb/c (Iffa Credo) mice were fused with cells of the mouse X63/Ag.8653 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Pack Size: 100 Tests
Prior to reconstitution store at +4oC following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 25 Tests
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P18827
Entrez Gene
SDC1
GO Terms
GO:0005887 integral to plasma membrane
GO:0005737 cytoplasm
GO:0005925 focal adhesion
GO:0006629 lipid metabolic process
GO:0008022 protein C-terminus binding
GO:0008092 cytoskeletal protein binding
GO:0042157 lipoprotein metabolic process
GO:0048627 myoblast development
GO:0055002 striated muscle cell development
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD138 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry Neat
Functional Assays 1
Immunofluorescence
Immunohistology - Frozen 1/100 1/500
Immunohistology - Paraffin 2 1/100 1/100 Pack Size: 2 ml
1/500 Pack Size: 0.1 mg, 0.1 ml
Western Blotting
Flow Cytometry Neat 1/5
  1. 1 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
  2. 2This product requires heat pre-treatment. Sodium citrate buffer pH6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Bone Marrow
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Functional Assays

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for CD138 antibody

  1. Borset, M. et al. (1993) Lack of IL-1 secretion from human myeloma cells highly purified by immunomagnetic separation.
    Br J Haematol. 85 (3): 446-51.
  2. Du, S. et al. (2010) Systemic mastocytosis in association with chronic lymphocytic leukemia and plasma cell myeloma.
    Int J Clin Exp Pathol. 3 (4): 448-57.
  3. Kylänpää, L. et al. (2009) Syndecan-1 and tenascin expression in cystic tumors of the pancreas.
    JOP. 10 (4): 378-82.
  4. Beauvais, D.M. et al. (2009) Syndecan-1 regulates alphavbeta3 and alphavbeta5 integrin activation during angiogenesis and is blocked by synstatin, a novel peptide inhibitor.
    J Exp Med. 206: 691-705.
  5. Beauvais, D.M. and Rapraeger, A.C. (2010) Syndecan-1 couples the insulin-like growth factor-1 receptor to inside-out integrin activation
    J Cell Sci. 123: 3796-807.
  6. Kim, Y.C. et al. (2010) Presence of Porphyromonas gingivalis and plasma cell dominance in gingival tissues with periodontitis.
    Oral Dis. 16: 375-81.
  7. Chang, H. et al. (2010) CKS1B nuclear expression is inversely correlated with p27Kip1 expression and is predictive of an adverse survival in patients with multiple myeloma.
    Haematologica. 95: 1542-7.
  8. Mahshid Y et al. (2009) High expression of 5-lipoxygenase in normal and malignant mantle zone B lymphocytes.
    BMC Immunol. 10: 2.
  9. Guedez, L. et al. (2005) Tissue inhibitor of metalloproteinase 1 (TIMP-1) promotes plasmablastic differentiation of a Burkitt lymphoma cell line: implications in the pathogenesis of plasmacytic/plasmablastic tumors.
    Blood. 105: 1660-8.
  10. Li, K. et al. (2010) Anaplastic lymphoma kinase-positive diffuse large B-cell lymphoma presenting as an isolated nasopharyngeal mass: a case report and review of literature.
    Int J Clin Exp Pathol. 4: 190-6.
  11. Yang, Y. et al. (2007) The syndecan-1 heparan sulfate proteoglycan is a viable target for myeloma therapy.
    Blood. 110: 2041-8.
  12. Thaunat, O. et al. (2010) Chronic rejection triggers the development of an aggressive intragraft immune response through recapitulation of lymphoid organogenesis.
    J Immunol. 185: 717-28.
  13. Cannizzo, E. et al. (2012) The role of CD19 and CD27 in the diagnosis of multiple myeloma by flow cytometry: a new statistical model.
    Am J Clin Pathol. 137 (3): 377-86.
  14. Li, K. et al. (2012) A rare and unique case of aggressive IgE-γ plasma cell myeloma in a 28-year-old woman presented initially as an orbital mass.
    Hum Pathol. 43: 2376-84.
  15. Christianson, H.C. et al. (2013) Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for their internalization and functional activity.
    Proc Natl Acad Sci U S A. 110 (43): 17380-5.
  16. Malminen, M. et al. (2002) Functional expression of NF1 tumor suppressor protein: association with keratin intermediate filaments during the early development of human epidermis.
    BMC Dermatol. 2: 10.
  17. Itoua Maïga, R. et al. (2014) Flow cytometry assessment of in vitro generated CD138+ human plasma cells.
    Biomed Res Int. 2014: 536482.
  18. Di Niro, R. et al. (2016) Responsive population dynamics and wide seeding into the duodenal lamina propria of transglutaminase-2-specific plasma cells in celiac disease.
    Mucosal Immunol. 9 (1): 254-64.
  19. Yigit, N. et al. (2015) Nuclear factor-erythroid 2, nerve growth factor receptor, and CD34-microvessel density are differentially expressed in primary myelofibrosis, polycythemia vera, and essential thrombocythemia.
    Hum Pathol. 46 (8): 1217-25.
  20. Lum, D. & Wong, K.P. (2006) Sarcomatoid plasmacytoma: a diagnosis not often considered.
    Pathology. 38 (6): 593-6.
  21. Gill, J. et al. (2009) A case of hyperIgG4 disease or IgG4-related sclerosing disease presenting as retroperitoneal fibrosis, chronic sclerosing sialadenitis and mediastinal lymphadenopathy.
    Pathology. 41 (3): 297-300.
  22. Adepu, S. et al. (2015) Incipient renal transplant dysfunction associates with tubular syndecan-1 expression and shedding.
    Am J Physiol Renal Physiol. 309 (2): F137-45.
  23. Hara, S. et al. (2016) Distribution and components of interstitial inflammation and fibrosis in IgG4-related kidney disease: Analysis of autopsy specimens
    Hum Pathol. May 28 [Epub ahead of print]
  24. Hosseini, A. et al. (2016) Morphometric analysis of inflammation in bronchial biopsies following exposure to inhaled diesel exhaust and allergen challenge in atopic subjects.
    Part Fibre Toxicol. 13: 2.
  25. Uenoyama, A. et al. (2016) Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.
    Biosci Biotechnol Biochem. 80 (7): 1344-55.
  26. Hourai, R. et al. (2017) IgG4-positive cell infiltration in various cardiovascular disorders - results from histopathological analysis of surgical samples.
    BMC Cardiovasc Disord. 17 (1): 52.
  27. Nagata, K. et al. (2017) Epstein-Barr Virus Lytic Reactivation Activates B Cells Polyclonally and Induces Activation-Induced Cytidine Deaminase Expression: A Mechanism Underlying Autoimmunity and Its Contribution to Graves' Disease.
    Viral Immunol. Mar 23. [Epub ahead of print]
  28. Tran, D.N. et al. (2017) Polychromatic flow cytometry is more sensitive than microscopy in detecting small monoclonal plasma cell populations.
    Cytometry B Clin Cytom. 92 (2): 136-144.
  29. Puchalapalli, M. et al. (2019) The Laminin-α1 Chain-Derived Peptide, AG73, Binds to Syndecans on MDA-231 Breast Cancer Cells and Alters Filopodium Formation.
    Anal Cell Pathol (Amst). 2019: 9192516.
  30. Mittal, S. et al. (2013) Lymphoid aggregates that resemble tertiary lymphoid organs define a specific pathological subset in metal-on-metal hip replacements.
    PLoS One. 8 (5): e63470.
  31. Egeland, N.G. et al. (2020) MiR-18a and miR-18b are expressed in the stroma of oestrogen receptor alpha negative breast cancers.
    BMC Cancer. 20 (1): 377.
  32. Forsberg, P.A. et al. (2019) Cellular proliferation by multiplex immunohistochemistry identifies aggressive disease behavior in relapsed multiple myeloma.
    Leuk Lymphoma. 60 (8): 2085-7.

Further Reading

  1. Anttonen, A. et al. (1999) Syndecan-1 expression has prognostic significance in head and neck carcinoma.
    Br J Cancer. 79 (3-4): 558-64.
  2. O'Connell, F.P. et al. (2004) CD138 (syndecan-1), a plasma cell marker immunohistochemical profile in hematopoietic and nonhematopoietic neoplasms.
    Am J Clin Pathol. 121:254-63.
  3. Sanderson, R.D. et al. (2008) Syndecan-1: a dynamic regulator of the myeloma microenvironment.
    Clin Exp Metastasis. 25:149-59.

Fluorescent Spectraviewer

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