CD130 antibody | A14-5C5

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Mouse anti Human CD130

Product Type
Monoclonal Antibody
Clone
A14-5C5
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6232GA
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human CD130 antibody, clone A14-5C5, recognizes CD130, also known as glycoprotein 130 (gp130) and STAT3. It is a ubiquitously expressed receptor subunit of the IL-6 family of cytokines and plays a role in many biological processes including hematopoiesis, immune response, inflammation, cardiovascular action, and neuronal survival. CD130 also plays a critical role during development with CD130 deficient mice being embryonically lethal as a result of myocardial hypoplasia and defects in hematopoiesis (White & Stephens 2011). CD130 is known to bind various cytokines in the IL-6 family, including IL-6, IL-11, IL-27, CT-1, oncostatin M and ciliary neurotrophic factor (White & Stephens 2011). Upon activation by these cytokines, various signaling pathways can be initiated including JAK/STAT, MAPK, mTOR, ERK and PI3K/Atk (Silver & Hunter 2010).

The purified Mouse anti Human CD130 antibody, clone A14-5C5 (MCA6232GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human CD130 antibody, clone E03-3C2 (MCA6233B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Sf21 derived recombinant human CD130 (Leu24-Glu619)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P40189
Entrez Gene
IL6ST
GO Terms
GO:0002675 positive regulation of acute inflammatory response
GO:0002821 positive regulation of adaptive immune response
GO:0005576 extracellular region
GO:0004897 ciliary neurotrophic factor receptor activity
GO:0005127 ciliary neurotrophic factor receptor binding
GO:0005615 extracellular space
GO:0005896 interleukin-6 receptor complex
GO:0005900 oncostatin-M receptor complex
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0010613 positive regulation of cardiac muscle hypertrophy
GO:0034097 response to cytokine stimulus
GO:0042102 positive regulation of T cell proliferation
GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0042803 protein homodimerization activity
GO:0045509 interleukin-27 receptor activity
GO:0045669 positive regulation of osteoblast differentiation
GO:0045768 positive regulation of anti-apoptosis
GO:0048861 leukemia inhibitory factor signaling pathway
GO:0070104 negative regulation of interleukin-6-mediated signaling pathway
GO:0070106 interleukin-27-mediated signaling pathway
GO:0070110 ciliary neurotrophic factor receptor complex
GO:0070120 ciliary neurotrophic factor-mediated signaling pathway
Regulatory
For research purposes only

Applications of CD130 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Fluorescent Spectraviewer

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