CD130 antibody | A14-5C5
Mouse anti Human CD130
- Product Type
- Monoclonal Antibody
- Clone
- A14-5C5
- Isotype
- IgG1
- Specificity
- CD130
| Mouse anti Human CD130 antibody, clone A14-5C5, recognizes CD130, also known as glycoprotein 130 (gp130) and STAT3. It is a ubiquitously expressed receptor subunit of the IL-6 family of cytokines and plays a role in many biological processes including hematopoiesis, immune response, inflammation, cardiovascular action, and neuronal survival. CD130 also plays a critical role during development with CD130 deficient mice being embryonically lethal as a result of myocardial hypoplasia and defects in hematopoiesis (White & Stephens 2011). CD130 is known to bind various cytokines in the IL-6 family, including IL-6, IL-11, IL-27, CT-1, oncostatin M and ciliary neurotrophic factor (White & Stephens 2011). Upon activation by these cytokines, various signaling pathways can be initiated including JAK/STAT, MAPK, mTOR, ERK and PI3K/Atk (Silver & Hunter 2010). The purified Mouse anti Human CD130 antibody, clone A14-5C5 (MCA6232GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human CD130 antibody, clone E03-3C2 (MCA6233B) as the detection antibody. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Sf21 derived recombinant human CD130 (Leu24-Glu619)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
- ELISA Matched Pair(s)
-
ELISA Matched Pair-Detection Antibody:
MCA6233B
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Synonyms
- IL-6RB
- UniProt
- P40189
- Entrez Gene
- IL6ST
- GO Terms
- GO:0002675 positive regulation of acute inflammatory response
- GO:0002821 positive regulation of adaptive immune response
- GO:0005576 extracellular region
- GO:0004897 ciliary neurotrophic factor receptor activity
- GO:0005127 ciliary neurotrophic factor receptor binding
- GO:0005615 extracellular space
- GO:0005896 interleukin-6 receptor complex
- GO:0005900 oncostatin-M receptor complex
- GO:0010575 positive regulation vascular endothelial growth factor production
- View More GO Terms
- GO:0010613 positive regulation of cardiac muscle hypertrophy
- GO:0034097 response to cytokine stimulus
- GO:0042102 positive regulation of T cell proliferation
- GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein
- GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
- GO:0042803 protein homodimerization activity
- GO:0045509 interleukin-27 receptor activity
- GO:0045669 positive regulation of osteoblast differentiation
- GO:0045768 positive regulation of anti-apoptosis
- GO:0048861 leukemia inhibitory factor signaling pathway
- GO:0070104 negative regulation of interleukin-6-mediated signaling pathway
- GO:0070106 interleukin-27-mediated signaling pathway
- GO:0070110 ciliary neurotrophic factor receptor complex
- GO:0070120 ciliary neurotrophic factor-mediated signaling pathway
MCA6232GA
100005244
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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