CD13 antibody | WM15
Low endotoxin Mouse anti Human CD13 antibody, clone WM15 (MCA1270EL) used to inhibit CD13 activity through ligation.
Image caption:
Effect of CD13 ligation on neutrophil and recombinant human CD13 activity.
Neutrophils were pre-incubated with bestatin (0.1 mM), anti-CD13 mAb WM-15 (8 μg/ml), anti-CD13 mAb MY7 (5 μg/ml), anti-CD13 mAb WM-47 (1:40 v/v) or vehicle control for 30 min. Cleavage of L-Alanine 4-nitroanilide (2 mM) was measured over 60 min and is expressed as a percentage of the activity observed with control treated cells. Data represent the mean ± SEM of n = 7 (Bestatin), n = 3 (WM-15 and WM-47) or mean ± SD of n = 2 independent experiments (MY7) each performed in triplicate (A). rCD13 (200 ng/ml) was pre-incubated alone or with increasing concentrations of bestatin for 30 min. Cleavage of L-Alanine 4-nitroanilide was measured over 60 min and converted to nmol substrate/min. The mean ±SEM from three independent experiments, each performed in triplicate, are shown. (B). ***, P <0.001, **, P <0.01, ns, non-significant vs. control, by Student's paired t test.
From: Fiddler CA, Parfrey H, Cowburn AS, Luo D, Nash GB, Murphy G, et al. (2016)
The Aminopeptidase CD13 Induces Homotypic Aggregation in Neutrophils and Impairs Collagen Invasion.
PLoS ONE 11(7): e0160108.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Phycoerythrin conjugated Mouse anti Human CD13 antibody, clone WM15 (MCA1270PE) used to evaluate CD13 expression on bone-marrow derived mesenchymal stem cells by flow cytometry
Image caption:
Immunophenotypic and differentiation characterization of BM-MSCs of aplastic anemia (AA) patients. (a) Representative flow cytometric histograms showing immunophenotype of BM-MSCs of AA patients and controls.
From: Chaturvedi CP, Tripathy NK, Minocha E, Sharma A, Rahman K, Nityanand S.
Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia. Stem Cells Int. 2018 Oct 17;2018:6901761.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
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- Product Type
- Monoclonal Antibody
- Clone
- WM15
- Isotype
- IgG1
- Specificity
- CD13
Antibody Quality and Performance Guarantee
| Mouse anti Human CD13 antibody, clone WM15 recognizes human CD13 also known as aminopeptidase N. CD13 is a single pass type II glycosylated integral membrane protein with a predicted molecular mass of ~110 kDa and an apparent molecular mass of ~150 kDa expressed by granulocytes, monocytes, fibroblasts, endothelial cells and by myeloid leukaemia cells (Bradstock et al. 1985). CD13 acts as a major cell surface receptor for group 1 coronoviruses (Breslin et al. 2003) which bind to a critical sequence encompassing amino acid residies 288-295 (Kolb et al. 1997). CD13 functions as an aminopeptidase enzyme, a metalloprotease present as both a membrane bound form and also a soluble aminopeptidase N. Mouse anti Human CD13, clone WM15 inhibits infection of cells by human coronavirus (Lachance et al. 1998) but not hepatitis C virus (Koutsoudakis et al. 2006) and inhibits aminopeptidase N activity of the CD13 molecule (Asmun et al. 1992). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Human AML cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice where fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Flow Cytometry | |
1/50 | 1/100 |
| Immunohistology - Frozen | |
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| Immunoprecipitation | |
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| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Bradstock, K.F. et al. (1985) Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells.
Pathology. 17 (3): 392-9.
References for CD13 antibody
-
Bradstock, K.F. et al. (1985) Myeloid progenitor surface antigen identified by monoclonal antibody.
Br J Haematol. 61 (1): 11-20. -
Favaloro, E.J. et al. (1988) Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b, CD-13 and CD-33.
Br J Haematol. 69 (2): 163-71. -
Favaloro, E.J. (1991) CD-13 (gp150; aminopeptidase-N): co-expression on endothelial and haemopoietic cells with conservation of functional activity.
Immunol Cell Biol. 69 ( Pt 4): 253-60. -
Favaloro, E.J. et al. (1993) The hepatobiliary disease marker serum alanine aminopeptidase predominantly comprises an isoform of the haematological myeloid differentiation antigen and leukaemia marker CD-13/gp150.
Clin Chim Acta. 220 (1): 81-90. -
Favaloro, E.J. et al. (1993) CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated.
Exp Hematol. 21 (13): 1695-701. -
Gredmark, S. et al. (2004) Human Cytomegalovirus Induces Inhibition of Macrophage Differentiation by Binding to Human Aminopeptidase N/CD13
J Immunol. 173: 4897-907 -
Thielitz, A. et al. (2004) Identification of extra- and intracellular alanyl aminopeptidases as new targets to modulate keratinocyte growth and differentiation.
Biochem Biophys Res Commun. 321 (4): 795-801. -
Lassnig, C. et al. (2005) Development of a transgenic mouse model susceptible to human coronavirus 229E.
Proc Natl Acad Sci U S A. 102 (23): 8275-80.
View The Latest Product References
-
Stolzing, A. et al. (2008) Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapies.
Mech Ageing Dev. 129: 163-73. -
Negussie, A.H. et al. (2010) Synthesis and in vitro evaluation of cyclic NGR peptide targeted thermally sensitive liposome.
J Control Release. 143: 265-73. -
Grzywacz, B. et al. (2011) Natural killer-cell differentiation by myeloid progenitors.
Blood. 117: 3548-58. -
Tavoosidana, G. et al. (2011) Multiple recognition assay reveals prostasomes as promising plasma biomarkers for prostate cancer.
Proc Natl Acad Sci U S A. 108: 8809-14. -
Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
J Biomed Biotechnol. 2012:172420. -
McCormack, E. et al. (2013) Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia.
Blood. 121 (7): e34-42. -
Fiddler, C.A. et al. (2016) The Aminopeptidase CD13 Induces Homotypic Aggregation in Neutrophils and Impairs Collagen Invasion.
PLoS One. 11 (7): e0160108. -
Chaturvedi, C.P. et al. (2018) Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia.
Stem Cells Int. 2018: 6901761. -
Menon, R. et al. (2023) Human Induced Pluripotent Stem Cell-Derived Pericytes as Scalable and Editable Source to Study Direct Lineage Reprogramming Into Induced Neurons.
Cell Reprogram. 25 (5): 212-23.
- Synonyms
- Aminopeptidase N
- RRID
- AB_321309
- UniProt
- P15144
- Entrez Gene
- ANPEP
- GO Terms
- GO:0001525 angiogenesis
- GO:0004177 aminopeptidase activity
- GO:0008237 metallopeptidase activity
- GO:0005829 cytosol
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005793 ER-Golgi intermediate compartment
- GO:0008270 zinc ion binding
- GO:0030154 cell differentiation
- View More GO Terms
- GO:0044419 interspecies interaction between organisms
MCA1270
166543
1709
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