CD126 antibody | B-R6
Mouse anti Human CD126 antibody, clone B-R6 used for the evaluation of IL6Rα expression on human muscle tissue by immunofluorescence.
Image caption:
Circulating IL-6 and muscle IL-6 mRNA and IL-6 receptor response muscle lengthening contractions (MLC).(1a) Average serum interleukin-6 (IL-6) concentration. Time 0 hr corresponds to pre-intervention values; all other time-points correspond to post-intervention time (hr). (1b) Relative IL-6 mRNA expression, expressed as fold-change from 0 hr. (1c) Pearson correlation of serum IL-6 concentration versus muscle IL-6 mRNA (fold-change), correlation is representative of the individual data points and presented as mean values (●)±SD (error bars). (1d) Relative IL-6 receptor (IL-6Rα) mRNA expression, expressed as fold-change from 0 hr. (1e) Immunofluorescent image (40×) of a muscle cross-section triple-stained for Pax7 (red), IL-6Rα (green) and nuclei (DAPI = blue); IL-6Rα staining is apparent on the sarcolemma and satellite cell membrane. White arrow denotes one of the Pax7+ nuclei which co-localized with IL-6Rα (scale bar = 100 μm). Values are reported as mean ±S.E.M. Mean values represent the mean for all 8 subjects per time-point (8 samples per time-point, 40 samples total). *p<0.05 vs. 0 hr.
From: McKay BR, De Lisio M, Johnston APW, O'Reilly CE, Phillips SM, Tarnopolsky MA, et al. (2009)
Association of Interleukin-6 Signalling with the Muscle Stem Cell Response Following Muscle-Lengthening Contractions in Humans.
PLoS ONE 4(6): e6027.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD126 antibody, clone B-R6 used for the evaluation of IL6Rα expression on human muscle tissue by immunofluorescence.
Image caption:
Control Images: (S5a): Immunofluorescent (IF) stain of 7 µm muscle cross-sections for IL-6Rα (green - streptavidin-FITC), nuclei (DAPI) and the secondary antibody used for Pax7 (secondary only - Alexa 594) acquired using the 20× objective. Arrows denote nuclei that co-localize with IL-6Rα and do not show any non-specific secondary binding of the Alexa 594, or any Alexa 594 interaction with the IL-6Rα antibody. (S5b): Immunofluorescent (IF) stain of 7 μm muscle cross-sections for Pax7 (red - Alexa 594), nuclei (DAPI) and the secondary antibody used for IL-6 (secondary - tertiary only: immunoglobulin biotinylated secondary antibody + streptavidin-FITC) acquired using the 20× objective. Arrows denote nuclei that co-localize with Pax7 and do not show any non-specific secondary binding of the secondary antibodies (FITC), or any FITC interaction with the Pax7 antibody. (S5c): Immunofluorescent (IF) stain of 7 μm muscle cross-sections for IL-6 (green - streptavidin FITC), nuclei (DAPI) and the secondary antibody used for Pax7 (secondary only - Alexa 594) acquired using the 20× objective. Arrows denote nuclei that co-localize with IL-6 and do not show any non-specific secondary binding of the Alexa 594, or any Alexa 594 interaction with the IL-6 antibody. (S5d): Immunofluorescent (IF) stain of 7 μm muscle cross-sections for PCNA (red - Texas Red), nuclei (DAPI) and the secondary antibody used for Pax7 (secondary only - Alexa 488) acquired using the 20× objective. Arrows denote nuclei that co-localize with PCNA and do not show any non-specific secondary binding of the Alexa 488, or any Alexa 488 interaction with the PCNA antibody. (S5e): Immunofluorescent (IF) stain of 7 μm muscle cross-sections for Pax7 (green - Alexa 488), nuclei (DAPI) and the secondary antibody used for PCNA (secondary only: Texas Red) acquired using the 20× objective. Arrows denote nuclei that co-localize with Pax7 and do not show any non-specific secondary binding of the secondary antibody (Texas Red), or any Texas Red interaction with the Pax7 antibody. (S5f): Immunofluorescent (IF) stain of 7 μm muscle cross-sections for phosphorylated STAT3 (p-STAT3) (red - Texas Red), nuclei (DAPI) and the secondary antibody used for Pax7 (secondary only - Alexa 488) acquired using the 20× objective. Arrows denote nuclei that co-localize with p-STAT3 and do not show any non-specific secondary binding of the Alexa 488, or any Alexa 488 interaction with the PCNA antibody.
From: McKay BR, De Lisio M, Johnston APW, O'Reilly CE, Phillips SM, Tarnopolsky MA, et al. (2009)
Association of Interleukin-6 Signalling with the Muscle Stem Cell Response Following Muscle-Lengthening Contractions in Humans.
PLoS ONE 4(6): e6027.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD126 antibody, clone B-R6 recognizes human Interleukin-6 receptor subunit alpha, also known as CD126, IL-6R or Membrane glycoprotein 80. Human CD126 is a 468 amino acid single pass type I transmembrane glycoprotein bearing a single Ig-like C2 type domain, two fibronectin type III domains and a single WSXWS motif which may be involved in receptor activation (Daqil et al. 2012).
Mouse anti Human CD126 antibody, clone B-R6 inhibits IL-6 mediated proliferation of XG-1 cells (Lu et al. 1995) and partially blocks binding of IL-6 to its receptor (Fourcin et al. 1994), clone B-R6 recognizes both the soluble and membranous human CD126
Removal of Sodium azide is recommended prior to use in functional assays. The use of EQU003 for this purpose.
Mouse anti Human CD126 antibody, clone B-R6 inhibits IL-6 mediated proliferation of XG-1 cells (Lu et al. 1995) and partially blocks binding of IL-6 to its receptor (Fourcin et al. 1994), clone B-R6 recognizes both the soluble and membranous human CD126
Removal of Sodium azide is recommended prior to use in functional assays. The use of EQU003 for this purpose.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- CHO cells transfected with cDNA of the IL-6 receptor.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cellls from immunised BALB/c (Iffa Credo) mice were fused with the X63/Ag.8653 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P08887 Related reagents
- Entrez Gene
- IL6R Related reagents
- GO Terms
- GO:0002384 hepatic immune response
- GO:0002446 neutrophil mediated immunity
- GO:0002548 monocyte chemotaxis
- GO:0002690 positive regulation of leukocyte chemotaxis
- GO:0006953 acute-phase response
- GO:0005615 extracellular space
- GO:0005896 interleukin-6 receptor complex
- GO:0050830 defense response to Gram-positive bacterium
- GO:0050829 defense response to Gram-negative bacterium
- GO:0010536 positive regulation of activation of Janus kinase activity
- GO:0016323 basolateral plasma membrane
- GO:0016324 apical plasma membrane
- GO:0042981 regulation of apoptosis
- GO:0019899 enzyme binding
- GO:0031018 endocrine pancreas development
- GO:0032717 negative regulation of interleukin-8 production
- GO:0032722 positive regulation of chemokine production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0032966 negative regulation of collagen biosynthetic process
- GO:0034097 response to cytokine stimulus
- GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
- GO:0042803 protein homodimerization activity
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0045669 positive regulation of osteoblast differentiation
- GO:0045768 positive regulation of anti-apoptosis
- GO:0048661 positive regulation of smooth muscle cell proliferation
- GO:0070102 interleukin-6-mediated signaling pathway
- GO:0070119 ciliary neurotrophic factor binding
- GO:0070120 ciliary neurotrophic factor-mediated signaling pathway
- Regulatory
- For research purposes only
Applications of CD126 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry | 1/50 | ||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F |
Product Specific References
References for CD126 antibody
-
Durandy, A. et al. (1994) Role of IL-6 in promoting growth of human EBV-induced B-cell tumors in severe combined immunodeficient mice.
J Immunol. 152 (11): 5361-7. -
Lu, Z.Y. et al. (1995) Interleukin-10 is a proliferation factor but not a differentiation factor for human myeloma cells.
Blood. 85 (9): 2521-7. -
Horiuchi, S. et al. (2006) Human T-cell leukemia virus type-I Tax induces expression of interleukin-6 receptor (IL-6R): Shedding of soluble IL-6R and activation of STAT3 signaling.
Int J Cancer. 119 (4): 823-30. -
McKay, B.R. et al. (2009) Association of interleukin-6 signalling with the muscle stem cell response following muscle-lengthening contractions in humans.
PLoS One. 4: e6027.
