CD126 antibody | B-R6

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Mouse anti Human CD126/Il-6R

Product Type
Monoclonal Antibody
Clone
B-R6
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA822 C E F IF IP 0.1 mg
Mouse anti Human CD126 antibody, clone B-R6 recognizes human Interleukin-6 receptor subunit alpha, also known as CD126, IL-6R or Membrane glycoprotein 80. Human CD126 is a 468 amino acid single pass type I transmembrane glycoprotein bearing a single Ig-like C2 type domain, two fibronectin type III domains and a single WSXWS motif which may be involved in receptor activation (Daqil et al. 2012).

Mouse anti Human CD126 antibody, clone B-R6 inhibits IL-6 mediated proliferation of XG-1 cells (Lu et al. 1995) and partially blocks binding of IL-6 to its receptor (Fourcin et al. 1994), clone B-R6 recognizes both the soluble and membranous human CD126

Removal of Sodium azide is recommended prior to use in functional assays. The use of EQU003 for this purpose.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
CHO cells transfected with cDNA of the IL-6 receptor.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cellls from immunised BALB/c (Iffa Credo) mice were fused with the X63/Ag.8653 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P08887 Related reagents
Entrez Gene
IL6R Related reagents
GO Terms
GO:0002384 hepatic immune response
GO:0002446 neutrophil mediated immunity
GO:0002548 monocyte chemotaxis
GO:0002690 positive regulation of leukocyte chemotaxis
GO:0006953 acute-phase response
GO:0005615 extracellular space
GO:0005896 interleukin-6 receptor complex
GO:0050830 defense response to Gram-positive bacterium
GO:0050829 defense response to Gram-negative bacterium
GO:0010536 positive regulation of activation of Janus kinase activity
GO:0016323 basolateral plasma membrane
GO:0016324 apical plasma membrane
GO:0042981 regulation of apoptosis
GO:0019899 enzyme binding
GO:0031018 endocrine pancreas development
GO:0032717 negative regulation of interleukin-8 production
GO:0032722 positive regulation of chemokine production
GO:0032755 positive regulation of interleukin-6 production
GO:0032966 negative regulation of collagen biosynthetic process
GO:0034097 response to cytokine stimulus
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0042803 protein homodimerization activity
GO:0043410 positive regulation of MAPKKK cascade
GO:0045669 positive regulation of osteoblast differentiation
GO:0045768 positive regulation of anti-apoptosis
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0070102 interleukin-6-mediated signaling pathway
GO:0070119 ciliary neurotrophic factor binding
GO:0070120 ciliary neurotrophic factor-mediated signaling pathway
Regulatory
For research purposes only

Applications of CD126 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/50
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Immunofluorescence

Product Specific References

References for CD126 antibody

  1. Durandy, A. et al. (1994) Role of IL-6 in promoting growth of human EBV-induced B-cell tumors in severe combined immunodeficient mice.
    J Immunol. 152 (11): 5361-7.
  2. Lu, Z.Y. et al. (1995) Interleukin-10 is a proliferation factor but not a differentiation factor for human myeloma cells.
    Blood. 85 (9): 2521-7.
  3. Horiuchi, S. et al. (2006) Human T-cell leukemia virus type-I Tax induces expression of interleukin-6 receptor (IL-6R): Shedding of soluble IL-6R and activation of STAT3 signaling.
    Int J Cancer. 119 (4): 823-30.
  4. McKay, B.R. et al. (2009) Association of interleukin-6 signalling with the muscle stem cell response following muscle-lengthening contractions in humans.
    PLoS One. 4: e6027.