CD126 antibody | B-R6
Mouse anti Human CD126 antibody, clone B-R6 used for the evaluation of IL6Rα expression on human muscle tissue by immunofluorescence.
Image caption:
Circulating IL-6 and muscle IL-6 mRNA and IL-6 receptor response muscle lengthening contractions (MLC).(1a) Average serum interleukin-6 (IL-6) concentration. Time 0 hr corresponds to pre-intervention values; all other time-points correspond to post-intervention time (hr). (1b) Relative IL-6 mRNA expression, expressed as fold-change from 0 hr. (1c) Pearson correlation of serum IL-6 concentration versus muscle IL-6 mRNA (fold-change), correlation is representative of the individual data points and presented as mean values (●)±SD (error bars). (1d) Relative IL-6 receptor (IL-6Rα) mRNA expression, expressed as fold-change from 0 hr. (1e) Immunofluorescent image (40×) of a muscle cross-section triple-stained for Pax7 (red), IL-6Rα (green) and nuclei (DAPI = blue); IL-6Rα staining is apparent on the sarcolemma and satellite cell membrane. White arrow denotes one of the Pax7+ nuclei which co-localized with IL-6Rα (scale bar = 100 μm). Values are reported as mean ±S.E.M. Mean values represent the mean for all 8 subjects per time-point (8 samples per time-point, 40 samples total). *p<0.05 vs. 0 hr.
From: McKay BR, De Lisio M, Johnston APW, O'Reilly CE, Phillips SM, Tarnopolsky MA, et al. (2009)
Association of Interleukin-6 Signalling with the Muscle Stem Cell Response Following Muscle-Lengthening Contractions in Humans.
PLoS ONE 4(6): e6027.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD126 antibody, clone B-R6 used for the evaluation of IL6Rα expression on human muscle tissue by immunofluorescence.
Image caption:
Control Images: (S5a): Immunofluorescent (IF) stain of 7 µm muscle cross-sections for IL-6Rα (green - streptavidin-FITC), nuclei (DAPI) and the secondary antibody used for Pax7 (secondary only - Alexa 594) acquired using the 20× objective. Arrows denote nuclei that co-localize with IL-6Rα and do not show any non-specific secondary binding of the Alexa 594, or any Alexa 594 interaction with the IL-6Rα antibody. (S5b): Immunofluorescent (IF) stain of 7 μm muscle cross-sections for Pax7 (red - Alexa 594), nuclei (DAPI) and the secondary antibody used for IL-6 (secondary - tertiary only: immunoglobulin biotinylated secondary antibody + streptavidin-FITC) acquired using the 20× objective. Arrows denote nuclei that co-localize with Pax7 and do not show any non-specific secondary binding of the secondary antibodies (FITC), or any FITC interaction with the Pax7 antibody. (S5c): Immunofluorescent (IF) stain of 7 μm muscle cross-sections for IL-6 (green - streptavidin FITC), nuclei (DAPI) and the secondary antibody used for Pax7 (secondary only - Alexa 594) acquired using the 20× objective. Arrows denote nuclei that co-localize with IL-6 and do not show any non-specific secondary binding of the Alexa 594, or any Alexa 594 interaction with the IL-6 antibody. (S5d): Immunofluorescent (IF) stain of 7 μm muscle cross-sections for PCNA (red - Texas Red), nuclei (DAPI) and the secondary antibody used for Pax7 (secondary only - Alexa 488) acquired using the 20× objective. Arrows denote nuclei that co-localize with PCNA and do not show any non-specific secondary binding of the Alexa 488, or any Alexa 488 interaction with the PCNA antibody. (S5e): Immunofluorescent (IF) stain of 7 μm muscle cross-sections for Pax7 (green - Alexa 488), nuclei (DAPI) and the secondary antibody used for PCNA (secondary only: Texas Red) acquired using the 20× objective. Arrows denote nuclei that co-localize with Pax7 and do not show any non-specific secondary binding of the secondary antibody (Texas Red), or any Texas Red interaction with the Pax7 antibody. (S5f): Immunofluorescent (IF) stain of 7 μm muscle cross-sections for phosphorylated STAT3 (p-STAT3) (red - Texas Red), nuclei (DAPI) and the secondary antibody used for Pax7 (secondary only - Alexa 488) acquired using the 20× objective. Arrows denote nuclei that co-localize with p-STAT3 and do not show any non-specific secondary binding of the Alexa 488, or any Alexa 488 interaction with the PCNA antibody.
From: McKay BR, De Lisio M, Johnston APW, O'Reilly CE, Phillips SM, Tarnopolsky MA, et al. (2009)
Association of Interleukin-6 Signalling with the Muscle Stem Cell Response Following Muscle-Lengthening Contractions in Humans.
PLoS ONE 4(6): e6027.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD126 antibody, clone B-R6 recognizes human Interleukin-6 receptor subunit alpha, also known as CD126, IL-6R or Membrane glycoprotein 80. Human CD126 is a 468 amino acid single pass type I transmembrane glycoprotein bearing a single Ig-like C2 type domain, two fibronectin type III domains and a single WSXWS motif which may be involved in receptor activation (Daqil et al. 2012).
Mouse anti Human CD126 antibody, clone B-R6 inhibits IL-6 mediated proliferation of XG-1 cells (Lu et al. 1995) and partially blocks binding of IL-6 to its receptor (Fourcin et al. 1994), clone B-R6 recognizes both the soluble and membranous human CD126
Removal of sodium azide is recommended prior to use in functional assays.
Mouse anti Human CD126 antibody, clone B-R6 inhibits IL-6 mediated proliferation of XG-1 cells (Lu et al. 1995) and partially blocks binding of IL-6 to its receptor (Fourcin et al. 1994), clone B-R6 recognizes both the soluble and membranous human CD126
Removal of sodium azide is recommended prior to use in functional assays.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- CHO cells transfected with cDNA of the IL-6 receptor.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cellls from immunised BALB/c (Iffa Credo) mice were fused with the X63/Ag.8653 mouse myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P08887
- Entrez Gene
- IL6R
- GO Terms
- GO:0002384 hepatic immune response
- GO:0002446 neutrophil mediated immunity
- GO:0002548 monocyte chemotaxis
- GO:0002690 positive regulation of leukocyte chemotaxis
- GO:0006953 acute-phase response
- GO:0005615 extracellular space
- GO:0005896 interleukin-6 receptor complex
- GO:0050830 defense response to Gram-positive bacterium
- GO:0050829 defense response to Gram-negative bacterium
- GO:0010536 positive regulation of activation of Janus kinase activity
- GO:0016323 basolateral plasma membrane
- GO:0016324 apical plasma membrane
- GO:0042981 regulation of apoptosis
- GO:0019899 enzyme binding
- GO:0031018 endocrine pancreas development
- GO:0032717 negative regulation of interleukin-8 production
- GO:0032722 positive regulation of chemokine production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0032966 negative regulation of collagen biosynthetic process
- GO:0034097 response to cytokine stimulus
- GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
- GO:0042803 protein homodimerization activity
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0045669 positive regulation of osteoblast differentiation
- GO:0045768 positive regulation of anti-apoptosis
- GO:0048661 positive regulation of smooth muscle cell proliferation
- GO:0070102 interleukin-6-mediated signaling pathway
- GO:0070119 ciliary neurotrophic factor binding
- GO:0070120 ciliary neurotrophic factor-mediated signaling pathway
- Regulatory
- For research purposes only
Applications of CD126 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry | 1/50 | ||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for CD126 antibody
-
Durandy, A. et al. (1994) Role of IL-6 in promoting growth of human EBV-induced B-cell tumors in severe combined immunodeficient mice.
J Immunol. 152 (11): 5361-7. -
Lu, Z.Y. et al. (1995) Interleukin-10 is a proliferation factor but not a differentiation factor for human myeloma cells.
Blood. 85 (9): 2521-7. -
Horiuchi, S. et al. (2006) Human T-cell leukemia virus type-I Tax induces expression of interleukin-6 receptor (IL-6R): Shedding of soluble IL-6R and activation of STAT3 signaling.
Int J Cancer. 119 (4): 823-30. -
McKay, B.R. et al. (2009) Association of interleukin-6 signalling with the muscle stem cell response following muscle-lengthening contractions in humans.
PLoS One. 4: e6027.
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