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CD122 antibody | TU27

Product Code Applications Pack Size List Price Your Price Qty
MCA6060GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader

Mouse anti Human CD122 recognizes CD122 also known as IL-2 receptor beta chain. CD122 is a 70-75 kDa transmembrane protein and member of the Ig superfamily. CD122 is primarily expressed by NK cells and but also by certain subsets of T cells. CD122 dimerises with either CD132 or CD25 to form IL-2 receptors. Activation of the IL-2 receptor induces signaling within MAPK, PI3K and JAK-STAT pathways.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
TL-Mor cell line.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells from the murine SP2/0-Ag14 non-secreting myeloma.
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/40
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul

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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

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Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

Source Reference

  1. Takeshita, T. et al. (1989) Monoclonal antibody defining a molecule possibly identical to the p75 subunit of interleukin 2 receptor.
    J Exp Med. 169 (4): 1323-32.

References for CD122 antibody

  1. Phillips, J.H. et al. (1989) Activation of natural killer cells via the p75 interleukin 2 receptor.
    J Exp Med. 170 (1): 291-6.
  2. Yagita, H. et al. (1989) Activation of peripheral blood T cells via the p75 interleukin 2 receptor.
    J Exp Med. 170 (4): 1445-50.
  3. Suzuki, J. et al. (1989) IL-2 receptor subunit, p75: direct demonstration of its IL-2 binding ability by using a novel monoclonal antibody.
    Int Immunol. 1 (4): 373-7.
  4. Espinoza-delgado, I. et al. (1990) Expression and role of p75 interleukin 2 receptor on human monocytes.
    J Exp Med. 171 (5): 1821-6.
  5. Starkey, P.M. (1991) Expression on cells of early human pregnancy decidua, of the p75, IL-2 and p145, IL-4 receptor proteins.
    Immunology. 73 (1): 64-70.
  6. Trentin, L. et al. (1992) Expression and functional role of the p75 interleukin 2 receptor chain on leukemic hairy cells.
    Cancer Res. 52 (19): 5223-8.

UniProt
P14784
Entrez Gene
IL2RB
GO Terms
GO:0004911 interleukin-2 receptor activity
GO:0005887 integral to plasma membrane
GO:0006461 protein complex assembly
GO:0009897 external side of plasma membrane
GO:0044419 interspecies interaction between organisms
GO:0045885 positive regulation of survival gene product expression

MCA6060GA

151938 166799

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