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CD122 antibody | TU27

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Mouse anti Human CD122

Mouse anti Human CD122:RPE

Product Type
Monoclonal Antibody
Clone
TU27
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6060GA
Datasheet
F
MSDS
0.1 mg loader

MCA6060PE
Datasheet
F
MSDS
100 Tests/0.5ml loader

Mouse anti Human CD122 recognizes CD122 also known as IL-2 receptor beta chain. CD122 is a 70-75 kDa transmembrane protein and member of of the Ig superfamily. CD122 is primarily expressed by NK cells and but also by certain subsets of T cells. CD122 dimerises with either CD132 or CD25 to form IL-2 receptors. Activation of the IL-2 receptor induces signaling within MAPK, PI3K and JAK-STAT pathways.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Immunogen
TL-Mor cell line
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells from the murine SP2/0-Ag14 non-secreting myeloma.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
12 months from date of despatch

More Information

UniProt
P14784 Related reagents
Entrez Gene
IL2RB Related reagents
GO Terms
GO:0004911 interleukin-2 receptor activity
GO:0005887 integral to plasma membrane
GO:0006461 protein complex assembly
GO:0009897 external side of plasma membrane
GO:0044419 interspecies interaction between organisms
GO:0045885 positive regulation of survival gene product expression
Regulatory
For research purposes only

Applications of CD122 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/40
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 5μl of the suggested working dilution to label 1x106 cells in 100μl
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader

Product Specific References

Source Reference

  1. Takeshita, T. et al. (1989) Monoclonal antibody defining a molecule possibly identical to the p75 subunit of interleukin 2 receptor.
    J Exp Med. 169 (4): 1323-32.

References for CD122 antibody

  1. Phillips, J.H. et al. (1989) Activation of natural killer cells via the p75 interleukin 2 receptor.
    J Exp Med. 170 (1): 291-6.
  2. Espinoza-delgado, I. et al. (1990) Expression and role of p75 interleukin 2 receptor on human monocytes.
    J Exp Med. 171 (5): 1821-6.
  3. Yagita, H. et al. (1989) Activation of peripheral blood T cells via the p75 interleukin 2 receptor.
    J Exp Med. 170 (4): 1445-50.
  4. Suzuki, J. et al. (1989) IL-2 receptor subunit, p75: direct demonstration of its IL-2 binding ability by using a novel monoclonal antibody.
    Int Immunol. 1 (4): 373-7.
  5. Starkey, P.M. (1991) Expression on cells of early human pregnancy decidua, of the p75, IL-2 and p145, IL-4 receptor proteins.
    Immunology. 73 (1): 64-70.
  6. Trentin, L. et al. (1992) Expression and functional role of the p75 interleukin 2 receptor chain on leukemic hairy cells.
    Cancer Res. 52 (19): 5223-8.
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