CD122 antibody | TU27

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Mouse anti Human CD122

Mouse anti Human CD122:RPE

Product Type: Monoclonal Antibody
Clone: TU27
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA6060GA	F			0.1 mg
MCA6060PE	F			100 Tests/0.5ml

Mouse anti Human CD122 recognizes CD122 also known as IL-2 receptor beta chain. CD122 is a 70-75 kDa transmembrane protein and member of of the Ig superfamily. CD122 is primarily expressed by NK cells and but also by certain subsets of T cells. CD122 dimerises with either CD132 or CD25 to form IL-2 receptors. Activation of the IL-2 receptor induces signaling within MAPK, PI3K and JAK-STAT pathways.

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Product Form: Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation: Purified IgG prepared by affinity chromatography
Preparation: Purified IgG prepared by affinity chromatography
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
0.2% Bovine Serum Albumin
Immunogen: TL-Mor cell line
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Fusion Partners: Spleen cells from immunized Balb/c mice were fused with cells from the murine SP2/0-Ag14 non-secreting myeloma.

Storage Information

Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch
Shelf Life: 12 months from date of despatch

More Information

UniProt: P14784 Related reagents
Entrez Gene: IL2RB Related reagents
GO Terms: GO:0004911 interleukin-2 receptor activity; GO:0005887 integral to plasma membrane; GO:0006461 protein complex assembly; GO:0009897 external side of plasma membrane; GO:0044419 interspecies interaction between organisms; GO:0045885 positive regulation of survival gene product expression
Regulatory: For research purposes only

Applications of CD122 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		1/10	1/40
Flow Cytometry		Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10 μl of the suggested working dilution to label 1x10⁶ cells in 100ul
Flow Cytometry: Use 5μl of the suggested working dilution to label 1x10⁶ cells in 100μl

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Product Specific References

Source Reference

Takeshita, T. et al. (1989) Monoclonal antibody defining a molecule possibly identical to the p75 subunit of interleukin 2 receptor.
J Exp Med. 169 (4): 1323-32.

References for CD122 antibody

Phillips, J.H. et al. (1989) Activation of natural killer cells via the p75 interleukin 2 receptor.
J Exp Med. 170 (1): 291-6.
Espinoza-delgado, I. et al. (1990) Expression and role of p75 interleukin 2 receptor on human monocytes.
J Exp Med. 171 (5): 1821-6.
Yagita, H. et al. (1989) Activation of peripheral blood T cells via the p75 interleukin 2 receptor.
J Exp Med. 170 (4): 1445-50.
Suzuki, J. et al. (1989) IL-2 receptor subunit, p75: direct demonstration of its IL-2 binding ability by using a novel monoclonal antibody.
Int Immunol. 1 (4): 373-7.
Starkey, P.M. (1991) Expression on cells of early human pregnancy decidua, of the p75, IL-2 and p145, IL-4 receptor proteins.
Immunology. 73 (1): 64-70.
Trentin, L. et al. (1992) Expression and functional role of the p75 interleukin 2 receptor chain on leukemic hairy cells.
Cancer Res. 52 (19): 5223-8.

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