CD120b antibody | 22221 (2/220)
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Mouse anti Human CD120b
- Product Type
- Monoclonal Antibody
- Clone
- 22221 (2/220)
- Isotype
- IgG2a
- Specificity
- CD120b
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| Mouse anti Human CD120b antibody, clone 22221 (2/220) Reacts with recombinant and native human soluble TNF RII. This antibody neutralizes the biological effect of rhTNF RII, but not rhTNF RI. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
5% Trehalose - Preservative Stabilisers
- None present.
- Immunogen
- E. coli-derived recombinant peptide corresponding to amino acids 23 to 257 of human CD120b
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Endotoxin Level
- < 0.1 EU/ug
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 1.0 µg/m |
| Functional Assays | |
||
| Immunohistology - Frozen | |
15 | 25 µg/ml |
| Western Blotting 1 | |
1.0 | 2.0 µg/ml |
- 1Using the concentration stated the detection limit is 10 ng/lane (reducing conditions)
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- ELISA
- Using the concentrations stated the detection limit is 5 ng/well
- Synonyms
- TNF-R2
- RRID
- AB_620274
- UniProt
- P20333
- Entrez Gene
- TNFRSF1B
- GO Terms
- GO:0005886 plasma membrane
- GO:0006915 apoptosis
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0005031 tumor necrosis factor receptor activity
- GO:0045121 membrane raft
View more products with CD120 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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