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CD11c antibody | BU15

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Anti Human CD11c Antibody, clone BU15 thumbnail image 1 Anti Human CD11c Antibody, clone BU15 thumbnail image 2 Anti Human CD11c Antibody, clone BU15 thumbnail image 3 Anti Human CD11c Antibody, clone BU15 thumbnail image 4
Anti Human CD11c Antibody, clone BU15 gallery image 1
Figure A. FITC conjugated Mouse anti Human HLA DP/DQ/DR antibody (MCA477F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Human HLA DP/DQ/DR antibody (MCA477F) and RPE conjugated Mouse anti Human CD11c (MCA2087PE). All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070)
Anti Human CD11c Antibody, clone BU15 gallery image 2
Published customer image:
AlexaFluor 647 conjugated Mouse anti Human CD11c antibody, clone BU15 (MCA2087GA) used for the identification of accessory cells derived from human PBMCs by flow cytometry.
Image caption:
Accessory Cells Are Activated by iRBCs PBMCs from ten malaria-naïve donors were cultured with live iRBCs or E. coli LPS for 24 h or left unstimulated. Surface expression of HLA-DR, CD40, and CD80 on accessory cells was analysed by flow cytometry.
Image Caption:
Accessory cells were defined as HLA-DR+ CD11c+ cells (A, left), and CD14+ monocytes and CD14− mDCs were selected for further analysis (A, right). Representative examples of surface expression levels of CD40, HLA-DR, and CD80 on HLA-DR+ CD11c+ CD14+ monocytes (B) and HLA-DR+ CD11c+ CD14− mDCs (C) are shown. Data are based on the collection of 100,000 total events. Results for all ten donors are summarised in (D). *, p < 0.05; **, p < 0.01; ***, p < 0.001; p-values are for paired t tests comparing stimulated and unstimulated cells from the same donor.

From: Newman KC, Korbel DS, Hafalla JC, Riley EM (2006)
Cross-Talk with Myeloid Accessory Cells Regulates Human Natural Killer Cell Interferon-γ Responses to Malaria.
PLoS Pathog 2(12): e118.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD11c Antibody, clone BU15 gallery image 3
Published customer image:
Mouse anti Human CD11c antibody, clone Bu15 (MCA2087) used for the evaluation of CD11c expression on Huvecs following co-culture with B. malayi by flow cytometry.
Image caption:
Presence of C3 enhances Mf adherence.
Level of B. malayi Mf adherence to HUVEC after co-culturing for 24 hours (A) in the presence of 10% intact human serum or 10% human serum depleted of C3. (B) Mf were incubated in intact sera and C3 deposition was detected using FITC conjugated anti-C3 Ab. (C) HUVEC were stimulated with or without IFN-γ (10 ng/ml) for 48 hours prior to a 24 hour incubation with Mf. HUVEC were then analysed by FACS for surface expression of CR1 or CD11c. Data show mean fluorescence intensity (MFI) of CR1 and CD11c. (D) HUVEC were pre-treated either with an isotype control antibody or with anti-CR1 for 45 minutes immediately prior to Mf co-culture for 24 hours. In another experiment, HUVEC were pre-treated for 45 minutes in the presence or absence of 250μg/ml mannan prior to Mf co-culture. Mf binding was counted after 24 hours of co-culture. Data are shown as the mean ± standard deviation of three independent experiments and Student's t-test was used to analyse statistical significance where ** p<0.01.

From: Zhou H, Luby-Phelps K, Mickey BE, Habib AA, Mason RP, Zhao D (2009)
Dynamic Near-Infrared Optical Imaging of 2-Deoxyglucose Uptake by Intracranial Glioma of Athymic Mice.
PLoS ONE 4(11): e8051.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD11c Antibody, clone BU15 gallery image 4
Detection of Mouse anti Human CD11c (MCA2087) in an immunohistochemical analysis of acetone fixed, frozen human tonsil section detected using the Histar Detection Kit (STAR3000A).

Mouse anti Human CD11c:Alexa Fluor® 488

Mouse anti Human CD11c:Alexa Fluor® 647

Mouse anti Human CD11c:FITC

Mouse anti Human CD11c

Mouse anti Human CD11c:RPE

Product Type
Monoclonal Antibody
Clone
BU15
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2087A488
Datasheet
F
SDS
100 Tests/1ml loader

MCA2087A647
Datasheet
F
SDS
100 Tests/1ml loader

MCA2087A647T
Datasheet
F
SDS
25 Tests/0.25ml loader

MCA2087F
Datasheet
F
SDS
0.1 mg loader

MCA2087FT
Datasheet
F
SDS
25 µg loader

MCA2087GA
Datasheet
C* F IP
SDS
0.1 mg loader

MCA2087T
Datasheet
C* F IP
SDS
25 µg loader

MCA2087
Datasheet
C* F IP
SDS
0.2 mg loader

MCA2087PE
Datasheet
F
SDS
100 Tests loader

MCA2087PET
Datasheet
F
SDS
25 Tests/0.25ml loader

Mouse anti Human CD11c antibody, clone Bu15 recognizes the integrin alphax subunit (ITGAX), a ~150 kDa glycoprotein also known as CD11 antigen-like family member C or Leu M5, CD11c is expressed by macrophages, monocytes, NK cells and most dendritic cells (Kohrgruber et al. 1999). CD11c is also expressed at a lower level by granulocytes.

CD11c forms a heterodimeric integral membrane protein with the integrin beta 2 chain to form the leukocyte specific integrin 'inactivated-C3b receptor 4'. CD11c interacts with a number of ligands including the G-P-R sequence in fibrinogen, ICAM-1 (Frick et al. 2005), iC3b (Sadhu et al. 2007) and Junctional adhesion molecule-like (Bazzoni et al. 2011). CD11c is involved in cell-cell interactions during the inflammatory process and is important for monocyte adhesion and chemotaxis. CD11c also acts as a signalling receptor for polysaccharide (Inqualls et al. 1995)

CD11c is expressed by hairy cell leukaemia cells (Goodman et al. 2003; Nicolaou et al. 2003).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 488-liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647- liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Pack Size: 100 Tests
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Pack Size: 25 Tests/0.25ml
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared from tissue culture supernatant
Preparation
Purified IgG prepared from tissue culture supernatant
Preparation
Purified IgG prepared from tissue culture supernatant
Preparation
Purified IgG prepared from tissue culture supernatant
Preparation
Purified IgG prepared from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
Pack Size: 100 Tests
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Pack Size: 25 Tests/0.25ml
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 100 Tests
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

This product should be stored undiluted.

DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 25 Tests/0.25ml
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P20702
Entrez Gene
ITGAX
GO Terms
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0007155 cell adhesion
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007229 integrin-mediated signaling pathway
GO:0009887 organ morphogenesis
GO:0050900 leukocyte migration
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD11c antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Flow Cytometry 1/10 1/50
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunoprecipitation
Flow Cytometry Neat 1/10
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 488 MCA928A488 F 100 Tests/1ml loader
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Flow Cytometry

Functional Assays

Product Specific References

References for CD11c antibody

  1. Hogg, N. et al. (1986) The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules.
    Eur J Immunol. 16 (3): 240-8.
  2. Newman, K.C. et al. (2006) Cross-talk with myeloid accessory cells regulates human natural killer cell interferon-gamma responses to malaria.
    PLoS Pathog. 2: e118.
  3. Herman, S. et al. (2012) Regulatory T cells form stable and long-lasting cell cluster with myeloid dendritic cells (DC).
    Int Immunol. 24 (7): 417-26.
  4. Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
    J Biomed Biotechnol. 2012:172420.
  5. Xie, Z. et al. (2016) Human umbilical cord-derived mesenchymal stem cells elicit macrophages into an anti-inflammatory phenotype to alleviate insulin resistance in type 2 diabetic rats.
    Stem Cells. 34 (3): 627-39.
  6. Brown, D.P. et al. (2009) The inhibitory receptor LILRB4 (ILT3) modulates antigen presenting cell phenotype and, along with LILRB2 (ILT4), is upregulated in response to Salmonella infection.
    BMC Immunol. 10: 56.
  7. Schroeder JH et al. (2017) Brugia malayi microfilariae adhere to human vascular endothelial cells in a C3-dependent manner.
    PLoS Negl Trop Dis. 11 (5): e0005592.
  8. Levy, O. et al. (2003) Critical role of the complement system in group B streptococcus-induced tumor necrosis factor alpha release.
    Infect Immun. 71: 6344-53.

Further Reading

  1. Larson, R.S. & Springer, T.A. (1990) Structure and function of leukocyte integrins.
    Immunol Rev. 114: 181-217.
  2. Loike, J.D. et al. (1991) CD11c/CD18 on neutrophils recognizes a domain at the N terminus of the A alpha chain of fibrinogen.
    Proc Natl Acad Sci U S A. 88 (3): 1044-8.
  3. Sanchez-Madrid, F. and Corbi, A.L. (1992) Leukocyte integrins: structure, function and regulation of their activity.
    Seminars Cell Biol. 3: 199-210.

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