CD11c antibody | BU15
AlexaFluor 647 conjugated Mouse anti Human CD11c antibody, clone BU15 (MCA2087GA) used for the identification of accessory cells derived from human PBMCs by flow cytometry.
Image caption:
Accessory Cells Are Activated by iRBCs PBMCs from ten malaria-naïve donors were cultured with live iRBCs or E. coli LPS for 24 h or left unstimulated. Surface expression of HLA-DR, CD40, and CD80 on accessory cells was analysed by flow cytometry.
Image Caption:
Accessory cells were defined as HLA-DR+ CD11c+ cells (A, left), and CD14+ monocytes and CD14− mDCs were selected for further analysis (A, right). Representative examples of surface expression levels of CD40, HLA-DR, and CD80 on HLA-DR+ CD11c+ CD14+ monocytes (B) and HLA-DR+ CD11c+ CD14− mDCs (C) are shown. Data are based on the collection of 100,000 total events. Results for all ten donors are summarised in (D). *, p < 0.05; **, p < 0.01; ***, p < 0.001; p-values are for paired t tests comparing stimulated and unstimulated cells from the same donor.
From: Newman KC, Korbel DS, Hafalla JC, Riley EM (2006) Cross-Talk with Myeloid Accessory Cells Regulates Human Natural Killer Cell Interferon-γ Responses to Malaria. PLoS Pathog 2(12): e118.
Mouse anti Human CD11c antibody, clone Bu15 (MCA2087) used for the evaluation of CD11c expression on Huvecs following co-culture with B. malayi by flow cytometry.
Image caption:
Presence of C3 enhances Mf adherence.
Level of B. malayi Mf adherence to HUVEC after co-culturing for 24 hours (A) in the presence of 10% intact human serum or 10% human serum depleted of C3. (B) Mf were incubated in intact sera and C3 deposition was detected using FITC conjugated anti-C3 Ab. (C) HUVEC were stimulated with or without IFN-γ (10 ng/ml) for 48 hours prior to a 24 hour incubation with Mf. HUVEC were then analysed by FACS for surface expression of CR1 or CD11c. Data show mean fluorescence intensity (MFI) of CR1 and CD11c. (D) HUVEC were pre-treated either with an isotype control antibody or with anti-CR1 for 45 minutes immediately prior to Mf co-culture for 24 hours. In another experiment, HUVEC were pre-treated for 45 minutes in the presence or absence of 250μg/ml mannan prior to Mf co-culture. Mf binding was counted after 24 hours of co-culture. Data are shown as the mean ± standard deviation of three independent experiments and Student's t-test was used to analyse statistical significance where ** p<0.01.
From: Zhou H, Luby-Phelps K, Mickey BE, Habib AA, Mason RP, Zhao D (2009)
Dynamic Near-Infrared Optical Imaging of 2-Deoxyglucose Uptake by Intracranial Glioma of Athymic Mice.
PLoS ONE 4(11): e8051.
Mouse anti Human CD11c:Alexa Fluor® 488
Mouse anti Human CD11c:Alexa Fluor® 647
Mouse anti Human CD11c:FITC
Mouse anti Human CD11c
Mouse anti Human CD11c:RPE
- Product Type
- Monoclonal Antibody
- Clone
- BU15
- Isotype
- IgG1
Mouse anti Human CD11c antibody, clone Bu15 recognizes the integrin alphax subunit (ITGAX), a ~150 kDa glycoprotein also known as CD11 antigen-like family member C or Leu M5, CD11c is expressed by macrophages, monocytes, NK cells and most dendritic cells (Kohrgruber et al. 1999). CD11c is also expressed at a lower level by granulocytes.
CD11c forms a heterodimeric integral membrane protein with the integrin beta 2 chain to form the leukocyte specific integrin 'inactivated-C3b receptor 4'. CD11c interacts with a number of ligands including the G-P-R sequence in fibrinogen, ICAM-1 (Frick et al. 2005), iC3b (Sadhu et al. 2007) and Junctional adhesion molecule-like (Bazzoni et al. 2011). CD11c is involved in cell-cell interactions during the inflammatory process and is important for monocyte adhesion and chemotaxis. CD11c also acts as a signalling receptor for polysaccharide (Inqualls et al. 1995)
CD11c is expressed by hairy cell leukaemia cells (Goodman et al. 2003; Nicolaou et al. 2003).
CD11c forms a heterodimeric integral membrane protein with the integrin beta 2 chain to form the leukocyte specific integrin 'inactivated-C3b receptor 4'. CD11c interacts with a number of ligands including the G-P-R sequence in fibrinogen, ICAM-1 (Frick et al. 2005), iC3b (Sadhu et al. 2007) and Junctional adhesion molecule-like (Bazzoni et al. 2011). CD11c is involved in cell-cell interactions during the inflammatory process and is important for monocyte adhesion and chemotaxis. CD11c also acts as a signalling receptor for polysaccharide (Inqualls et al. 1995)
CD11c is expressed by hairy cell leukaemia cells (Goodman et al. 2003; Nicolaou et al. 2003).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488-liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647- liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Pack Size: 25 Tests/0.25mlPurified IgG conjugated to R. Phycoerythrin (RPE) - liquidPack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared from tissue culture supernatant
- Preparation
- Purified IgG prepared from tissue culture supernatant
- Preparation
- Purified IgG prepared from tissue culture supernatant
- Preparation
- Purified IgG prepared from tissue culture supernatant
- Preparation
- Purified IgG prepared from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
- Pack Size: 25 Tests/0.25ml0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% SucrosePack Size: 100 Tests0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 25 Tests/0.25mlStore at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.
This product should be stored undiluted.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P20702 Related reagents
- Entrez Gene
- ITGAX Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0007596 blood coagulation
- GO:0007155 cell adhesion
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007229 integrin-mediated signaling pathway
- GO:0009887 organ morphogenesis
- GO:0050900 leukocyte migration
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD11c antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/10 | 1/50 | |
| Immunohistology - Frozen 1 | |||
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat | 1/10 |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | 100 Tests/1ml | F | ||
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | 100 Tests/1ml | F | ||
| Mouse IgG1 Negative Control:FITC | MCA928F | 100 Tests | F | ||
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F | ||
| Mouse IgG1 Negative Control:RPE | MCA928PE | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F |
Product Specific References
References for CD11c antibody
-
Hogg, N. et al. (1986) The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules.
Eur J Immunol. 16 (3): 240-8. -
Loike, J.D. et al. (1991) CD11c/CD18 on neutrophils recognizes a domain at the N terminus of the A alpha chain of fibrinogen.
Proc Natl Acad Sci U S A. 88 (3): 1044-8. -
Newman, K.C. et al. (2006) Cross-talk with myeloid accessory cells regulates human natural killer cell interferon-gamma responses to malaria.
PLoS Pathog. 2: e118. -
Sanchez-Madrid, F. and Corbi, A.L. (1992) Leukocyte integrins: structure, function and regulation of their activity.
Seminars Cell Biol. 3: 199-210. -
Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
J Biomed Biotechnol. 2012:172420. -
Bird, R.C. et al. (2011) An autologous dendritic cell canine mammary tumor hybrid-cell fusion vaccine.
Cancer Immunol Immunother. 60 (1): 87-97. -
Eisenhardt, S.U. et al. (2007) Generation of activation-specific human anti-alphaMbeta2 single-chain antibodies as potential diagnostic tools and therapeutic agents.
Blood. 109: 3521-8. -
Herman, S. et al. (2012) Regulatory T cells form stable and long-lasting cell cluster with myeloid dendritic cells (DC).
Int Immunol. 24 (7): 417-26. -
Levy, O. et al. (2003) Critical role of the complement system in group B streptococcus-induced tumor necrosis factor alpha release.
Infect Immun. 71: 6344-53. -
Xie, Z. et al. (2016) Human umbilical cord-derived mesenchymal stem cells elicit macrophages into an anti-inflammatory phenotype to alleviate insulin resistance in type 2 diabetic rats.
Stem Cells. 34 (3): 627-39. -
Brown, D.P. et al. (2009) The inhibitory receptor LILRB4 (ILT3) modulates antigen presenting cell phenotype and, along with LILRB2 (ILT4), is upregulated in response to Salmonella infection.
BMC Immunol. 10: 56. -
Schroeder JH et al. (2017) Brugia malayi microfilariae adhere to human vascular endothelial cells in a C3-dependent manner.
PLoS Negl Trop Dis. 11 (5): e0005592.
Further Reading
-
Larson, R.S. & Springer, T.A. (1990) Structure and function of leukocyte integrins.
Immunol Rev. 114: 181-217.
