CD11c antibody | BU15
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Mouse anti Human CD11c
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD11c antibody, clone Bu15 recognizes the integrin alphax subunit (ITGAX), a ~150 kDa glycoprotein also known as CD11 antigen-like family member C or Leu M5, CD11c is expressed by macrophages, monocytes, NK cells and most dendritic cells (Kohrgruber et al. 1999). CD11c is also expressed at a lower level by granulocytes.
CD11c forms a heterodimeric integral membrane protein with the integrin beta 2 chain to form the leukocyte specific integrin 'inactivated-C3b receptor 4'. CD11c interacts with a number of ligands including the G-P-R sequence in fibrinogen, ICAM-1 (Frick et al. 2005), iC3b (
CD11c is expressed by hairy cell leukaemia cells (Goodman et al. 2003; Nicolaou et al. 2003).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Cynomolgus monkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen 1|
|Immunohistology - Paraffin|
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD11c antibody
Hogg, N. et al. (1986) The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules.
Eur J Immunol. 16 (3): 240-8.
Levy, O. et al. (2003) Critical role of the complement system in group B streptococcus-induced tumor necrosis factor alpha release.
Infect Immun. 71: 6344-53.
Newman, K.C. et al. (2006) Cross-talk with myeloid accessory cells regulates human natural killer cell interferon-gamma responses to malaria.
PLoS Pathog. 2: e118.
Brown, D.P. et al. (2009) The inhibitory receptor LILRB4 (ILT3) modulates antigen presenting cell phenotype and, along with LILRB2 (ILT4), is upregulated in response to Salmonella infection.
BMC Immunol. 10: 56.
Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
J Biomed Biotechnol. 2012:172420.
Herman, S. et al. (2012) Regulatory T cells form stable and long-lasting cell cluster with myeloid dendritic cells (DC).
Int Immunol. 24 (7): 417-26.
Xie, Z. et al. (2016) Human umbilical cord-derived mesenchymal stem cells elicit macrophages into an anti-inflammatory phenotype to alleviate insulin resistance in type 2 diabetic rats.
Stem Cells. 34 (3): 627-39.
Schroeder JH et al. (2017) Brugia malayi microfilariae adhere to human vascular endothelial cells in a C3-dependent manner.
PLoS Negl Trop Dis. 11 (5): e0005592.
View The Latest Product References
Simões, R.D. et al. (2019) Effects of Regulatory T Cell Depletion on NK Cell Responses against Listeria monocytogenes in Feline Immunodeficiency Virus Infected Cats.
Viruses. 11 (11)Oct 24 [Epub ahead of print].
Larson, R.S. & Springer, T.A. (1990) Structure and function of leukocyte integrins.
Immunol Rev. 114: 181-217.
Loike, J.D. et al. (1991) CD11c/CD18 on neutrophils recognizes a domain at the N terminus of the A alpha chain of fibrinogen.
Proc Natl Acad Sci U S A. 88 (3): 1044-8.
Sanchez-Madrid, F. and Corbi, A.L. (1992) Leukocyte integrins: structure, function and regulation of their activity.
Seminars Cell Biol. 3: 199-210.
- Integrin Alpha X Chain
- Entrez Gene
- GO Terms
- GO:0005515 protein binding
- GO:0007596 blood coagulation
- GO:0007155 cell adhesion
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007229 integrin-mediated signaling pathway
- GO:0009887 organ morphogenesis
- GO:0050900 leukocyte migration
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