CD11c antibody | BU15




Mouse anti Human CD11c:Alexa Fluor® 488
Mouse anti Human CD11c:Alexa Fluor® 647
Mouse anti Human CD11c:FITC
Mouse anti Human CD11c
Mouse anti Human CD11c:RPE
- Product Type
- Monoclonal Antibody
- Clone
- BU15
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA2087A488 | F | 100 Tests/1ml |
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MCA2087A647T | F | 25 Tests/0.25ml |
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MCA2087A647 | F | 100 Tests/1ml |
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MCA2087FT | F | 25 µg |
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MCA2087F | F | 0.1 mg |
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MCA2087T | C* F IP | 25 µg |
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MCA2087GA | C* F IP | 0.1 mg |
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MCA2087 | C* F IP | 0.2 mg |
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MCA2087PET | F | 25 Tests/0.25ml |
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MCA2087PE | F | 100 Tests |
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CD11c forms a heterodimeric integral membrane protein with the integrin beta 2 chain to form the leukocyte specific integrin 'inactivated-C3b receptor 4'. CD11c interacts with a number of ligands including the G-P-R sequence in fibrinogen, ICAM-1 (Frick et al. 2005), iC3b (
CD11c is expressed by hairy cell leukaemia cells (Goodman et al. 2003; Nicolaou et al. 2003).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488-liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647- liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Pack Size: 25 Tests/0.25mlPurified IgG conjugated to R. Phycoerythrin (RPE) - liquidPack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared from tissue culture supernatant
- Preparation
- Purified IgG prepared from tissue culture supernatant
- Preparation
- Purified IgG prepared from tissue culture supernatant
- Preparation
- Purified IgG prepared from tissue culture supernatant
- Preparation
- Purified IgG prepared from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
- Pack Size: 25 Tests/0.25ml0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% SucrosePack Size: 100 Tests0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 25 Tests/0.25mlStore at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.
This product should be stored undiluted.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P20702
- Entrez Gene
- ITGAX
- GO Terms
- GO:0005515 protein binding
- GO:0007596 blood coagulation
- GO:0007155 cell adhesion
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007229 integrin-mediated signaling pathway
- GO:0009887 organ morphogenesis
- GO:0050900 leukocyte migration
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD11c antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | 1/10 | 1/50 | |
Immunohistology - Frozen 1 | |||
Immunohistology - Paraffin | |||
Immunoprecipitation | |||
Flow Cytometry | Neat | 1/10 |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | F | 100 Tests/1ml |
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Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
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Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD11c antibody
-
Hogg, N. et al. (1986) The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules.
Eur J Immunol. 16 (3): 240-8. -
Newman, K.C. et al. (2006) Cross-talk with myeloid accessory cells regulates human natural killer cell interferon-gamma responses to malaria.
PLoS Pathog. 2: e118. -
Herman, S. et al. (2012) Regulatory T cells form stable and long-lasting cell cluster with myeloid dendritic cells (DC).
Int Immunol. 24 (7): 417-26. -
Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
J Biomed Biotechnol. 2012:172420. -
Xie, Z. et al. (2016) Human umbilical cord-derived mesenchymal stem cells elicit macrophages into an anti-inflammatory phenotype to alleviate insulin resistance in type 2 diabetic rats.
Stem Cells. 34 (3): 627-39. -
Brown, D.P. et al. (2009) The inhibitory receptor LILRB4 (ILT3) modulates antigen presenting cell phenotype and, along with LILRB2 (ILT4), is upregulated in response to Salmonella infection.
BMC Immunol. 10: 56. -
Schroeder JH et al. (2017) Brugia malayi microfilariae adhere to human vascular endothelial cells in a C3-dependent manner.
PLoS Negl Trop Dis. 11 (5): e0005592. -
Levy, O. et al. (2003) Critical role of the complement system in group B streptococcus-induced tumor necrosis factor alpha release.
Infect Immun. 71: 6344-53.
Further Reading
-
Larson, R.S. & Springer, T.A. (1990) Structure and function of leukocyte integrins.
Immunol Rev. 114: 181-217. -
Loike, J.D. et al. (1991) CD11c/CD18 on neutrophils recognizes a domain at the N terminus of the A alpha chain of fibrinogen.
Proc Natl Acad Sci U S A. 88 (3): 1044-8. -
Sanchez-Madrid, F. and Corbi, A.L. (1992) Leukocyte integrins: structure, function and regulation of their activity.
Seminars Cell Biol. 3: 199-210.
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