CD104 antibody | 450-9D


Mouse anti Human CD104:Preservative Free
Mouse anti Human CD104:FITC
Mouse anti Human CD104
- Product Type
- Monoclonal Antibody
- Clone
- 450-9D
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1456XZ | C* F IF IP WB | 1 mg |
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MCA1456F | F IF | 0.1 mg |
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MCA1456T | C* F IF IP WB | 25 µg |
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MCA1456 | C* F IF IP WB | 0.2 mg |
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Mouse anti Human CD104 antibody, clone 450-9D recognizes an extracellular epitope on the CD104 molecule.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mustelid Ferret - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Normal saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Purified alpha6 beta4 integrin from A431 cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- P16144 Related reagents
- Entrez Gene
- ITGB4 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007154 cell communication
- GO:0007160 cell-matrix adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0007275 multicellular organismal development
- GO:0031581 hemidesmosome assembly
- Regulatory
- For research purposes only
Applications of CD104 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/25 | 1/100 | |
Immunofluorescence | |||
Immunohistology - Frozen 1 | 1/500 | 1/1000 | |
Immunohistology - Paraffin | |||
Immunoprecipitation | |||
Western Blotting | |||
Flow Cytometry | Neat | 1/10 | |
Immunofluorescence | |||
Flow Cytometry | 1/25 | 1/100 | |
Immunofluorescence | |||
Immunohistology - Frozen 1 | 1/500 | 1/1000 | |
Immunohistology - Paraffin | |||
Immunoprecipitation | |||
Western Blotting |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Human Tonsil
- Histology Positive Control Tissue
- Human Tonsil
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD104 antibody
-
Kennel, S.J. et al. (1990) Second generation monoclonal antibodies to the human integrin alpha 6 beta 4.
Hybridoma. 9 (3): 243-55. -
Barbaro, V. et al. (2009) Reconstruction of a human hemicornea through natural scaffolds compatible with the growth of corneal epithelial stem cells and stromal keratocytes.
Mol Vis. 15: 2084-93. -
Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
Vet Immunol Immunopathol. 132:109-15. -
Smith, K. et al. (2011) Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.
Immunology. 132: 256-65. -
Alam, H. et al. (2011) Loss of keratins 8 and 18 leads to alterations in α6β4-integrin-mediated signalling and decreased neoplastic progression in an oral-tumour-derived cell line.
J Cell Sci. 124: 2096-106. -
Lai-cheong, J.E. et al. (2009) Loss-of-function FERMT1 mutations in kindler syndrome implicate a role for fermitin family homolog-1 in integrin activation.
Am J Pathol. 175 (4): 1431-41. -
Groves, R.W. et al. (2010) A homozygous nonsense mutation within the dystonin gene coding for the coiled-coil domain of the epithelial isoform of BPAG1 underlies a new subtype of autosomal recessive epidermolysis bullosa simplex.
J Invest Dermatol. 130 (6): 1551-7.