CD104 antibody | 450-9D
Mouse anti Human CD104 antibody, clone 450-9D (MCA1456) showing β4 integrin expression in reconstructed hemicorneal section by immunofluorescence.
Image caption:
Extract from Fig 1 showing β4 immunofluorescence:
Expression of epithelial cell markers in reconstructed hemicorneas. Human corneal epithelial stem cells seeded onto the HKL scaffold form a pluristratified and differentiated epithelium. Cryosections were analyzed through immunofluorescence. The layer of basal cuboidal cells was firmly attached to the underlying extra cellular matrix (ECM) and to the basement membrane through integrins α6 (blue, A), β1 (green, B), and β4 (red, C). The basal expression of p63 isoforms (red, D) and of the stem cell marker ΔNp63α (yellow, E) was always observed, thus suggesting the maintenance of undifferentiated progenitor cells interspersed between differentiated cells. Expression of keratin 14 (green, F) was also observed in the basal layers. Corneal differentiation occurred in all epithelial layers and was evaluated through the analysis of several markers, including 14-3-3σ (pink, G), keratin 19 (orange, H), keratin 3 (yellow, I), and keratin 12 (red, J). Note the presence of the Bowman's membrane in transmitted-light images (white arrows, D–E). Scale bar=50 μm.
From: Barbaro V, Ferrari S, Fasolo A, Ponzin D, Di Iorio E.
Reconstruction of a human hemicornea through natural scaffolds compatible with the growth of corneal epithelial stem cells and stromal keratocytes.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD104
- Product Type
- Monoclonal Antibody
- Clone
- 450-9D
- Isotype
- IgG1
- Specificity
- CD104
Antibody Quality and Performance Guarantee
| Mouse anti Human CD104 antibody, clone 450-9D recognizes the human beta4 integrin, also known as CD104. CD104 is a ~205 kDa glycoprotein which associates with the alpha6 integrin to form the alpha6/beta4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumor cell lines. Mouse anti Human CD104 antibody, clone 450-9D recognizes an extracellular epitope on the CD104 molecule. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mustelid Expected from Sequence Ferret Mink - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified alpha6 beta4 integrin from A431 cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/100 |
| Immunofluorescence | |
||
| Immunohistology - Frozen 1 | |
1/500 | 1/1000 |
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |
||
| Western Blotting | |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
- Histology Positive Control Tissue
- Human tonsil
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Kennel, S.J. et al. (1990) Second generation monoclonal antibodies to the human integrin alpha 6 beta 4.
Hybridoma. 9 (3): 243-55.
References for CD104 antibody
-
Aasted, B. & Viuff, B. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
Vet Immunol Immunopathol. 119 (1-2): 27-37. -
Barbaro, V. et al. (2009) Reconstruction of a human hemicornea through natural scaffolds compatible with the growth of corneal epithelial stem cells and stromal keratocytes.
Mol Vis. 15: 2084-93. -
Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
Vet Immunol Immunopathol. 132:109-15. -
Lai-cheong, J.E. et al. (2009) Loss-of-function FERMT1 mutations in kindler syndrome implicate a role for fermitin family homolog-1 in integrin activation.
Am J Pathol. 175 (4): 1431-41. -
Groves, R.W. et al. (2010) A homozygous nonsense mutation within the dystonin gene coding for the coiled-coil domain of the epithelial isoform of BPAG1 underlies a new subtype of autosomal recessive epidermolysis bullosa simplex.
J Invest Dermatol. 130 (6): 1551-7. -
Smith, K. et al. (2011) Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.
Immunology. 132: 256-65. -
Alam, H. et al. (2011) Loss of keratins 8 and 18 leads to alterations in α6β4-integrin-mediated signalling and decreased neoplastic progression in an oral-tumour-derived cell line.
J Cell Sci. 124: 2096-106.
- Synonyms
- Integrin Beta 4
- RRID
- AB_2129143
- UniProt
- P16144
- Entrez Gene
- ITGB4
- GO Terms
- GO:0005515 protein binding
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007154 cell communication
- GO:0007160 cell-matrix adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0007275 multicellular organismal development
- GO:0031581 hemidesmosome assembly
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