CD103 antibody | LF61
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Mouse anti Human CD103
- Product Type
- Monoclonal Antibody
- Clone
- LF61
- Isotype
- IgG1
- Specificity
- CD103
Antibody Quality and Performance Guarantee
| Mouse anti Human CD103 antibody, clone LF61 recognizes the human CD103 cell surface antigen, a glycoprotein expressed by approximately 1% of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukemia cells. The antigen is also expressed by intraepithelial lymphocytes. It has recently been shown to be identical to the alpha E integrin. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Hairy cell leukemia cells.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
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| Immunohistology - Frozen 1 | |
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| Immunohistology - Paraffin | |||
| Immunoprecipitation | |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Flenghi, L. et al. (1990) LF61: a new monoclonal antibody directed against a trimeric molecule (150 kDa, 125 kDa, 105 kDa) associated with hairy cell leukaemia.
Br J Haematol. 76 (4): 451-9.
References for CD103 antibody
-
Falini, B. et al. (1990) Selection of a panel of monoclonal antibodies for monitoring residual disease in peripheral blood and bone marrow of interferon-treated hairy cell leukaemia patients.
Br J Haematol. 76 (4): 460-8. -
Ling, K.L. et al. (2007) Modulation of CD103 expression on human colon carcinoma-specific CTL.
J Immunol. 178: 2908-15. -
Hadley, G.A. et al. (2001) CD103+ CTL accumulate within the graft epithelium during clinical renal allograft rejection.
Transplantation. 72 (9): 1548-55.
- RRID
- AB_324525
- UniProt
- P38570
- Entrez Gene
- ITGAE
- GO Terms
- GO:0007155 cell adhesion
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007229 integrin-mediated signaling pathway
View more products with CD103 specificity
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