CD103 antibody | LF61

Mouse anti Human CD103

Product Type
Monoclonal Antibody
Clone
LF61
Isotype
IgG1
Specificity
CD103

Product Code Applications Pack Size List Price Your Price Qty
MCA1416G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IP 0.2 mg loader
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loader
MCA1416GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IP 0.1 mg loader
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loader
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Mouse anti Human CD103 antibody, clone LF61 recognizes the human CD103 cell surface antigen, a glycoprotein expressed by approximately 1% of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukemia cells. The antigen is also expressed by intraepithelial lymphocytes. It has recently been shown to be identical to the alpha E integrin.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Hairy cell leukemia cells
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunoprecipitation
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control

Source Reference

  1. Flenghi, L. et al. (1990) LF61: a new monoclonal antibody directed against a trimeric molecule (150 kDa, 125 kDa, 105 kDa) associated with hairy cell leukaemia.
    Br J Haematol. 76 (4): 451-9.

References for CD103 antibody

  1. Falini, B. et al. (1990) Selection of a panel of monoclonal antibodies for monitoring residual disease in peripheral blood and bone marrow of interferon-treated hairy cell leukaemia patients.
    Br J Haematol. 76 (4): 460-8.
  2. Ling, K.L. et al. (2007) Modulation of CD103 expression on human colon carcinoma-specific CTL.
    J Immunol. 178: 2908-15.
  3. Hadley, G.A. et al. (2001) CD103+ CTL accumulate within the graft epithelium during clinical renal allograft rejection.
    Transplantation. 72 (9): 1548-55.

RRID
AB_324525
UniProt
P38570
Entrez Gene
ITGAE
GO Terms
GO:0007155 cell adhesion
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007229 integrin-mediated signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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