CD10 antibody | 56C6
Mouse anti Human CD10 antibody, clone 56C6 recognizes human Neprilysin, also known as CD10, Atriopeptidase, Common acute lymphocytic leukemia antigen (CALLA), Enkephalinase, Neutral endopeptidase 24.11, Neutral endopeptidase or Skin fibroblast elastase. CD10 is a 749 amino acid, ~100 kDa single pass type II transmembrane glycoprotein, expressed on a variety of cell types including immature and germinal centre B cells, lymphoblastic leukaemia cells and also on the brush border of kidney epithelial cells.
Mutations in the MME gene (encoding CD10) are involved in the development of Charcot-Marie-Tooth disease 2T (CMT2T), a disorder of the peripheral nervous system, demonstrating progerssive peripheral muscle weakness and atrophy (Higuchi et al. 2016). Mutations of the MME gene can also lead to Spinocerebellar ataxia 43 (SCA43) characterized by a progressive loss of gait coordination along with hand, speech and eye coordination. Degeneration of the cerebellum, with variable involvement of the brain stem and spinal cord are apparent in this slow progressive autosomal dominant form of the disease (Depondt et al. 2016).
Mutations in the MME gene (encoding CD10) are involved in the development of Charcot-Marie-Tooth disease 2T (CMT2T), a disorder of the peripheral nervous system, demonstrating progerssive peripheral muscle weakness and atrophy (Higuchi et al. 2016). Mutations of the MME gene can also lead to Spinocerebellar ataxia 43 (SCA43) characterized by a progressive loss of gait coordination along with hand, speech and eye coordination. Degeneration of the cerebellum, with variable involvement of the brain stem and spinal cord are apparent in this slow progressive autosomal dominant form of the disease (Depondt et al. 2016).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Recombinant human CD10.
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P08473 Related reagents
- Entrez Gene
- MME Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0004222 metalloendopeptidase activity
- GO:0005887 integral to plasma membrane
- GO:0046872 metal ion binding
- GO:0006508 proteolysis
- GO:0007267 cell-cell signaling
- Regulatory
- For research purposes only
Applications of CD10 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | 1/80 | ||
| Immunohistology - Paraffin 1 | 1/80 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Tonsil, Intestine or kidney
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for CD10 antibody
-
Angelin-Duclos, C. et al. (2000) Commitment of B lymphocytes to a plasma cell fate is associated with Blimp-1 expression in vivo.
J Immunol. 165 (10): 5462-71. -
Carvounis, E.E. et al. (2003) Undifferentiated carcinoma with osteoclast-like giant cells of the pancreas.
Int J Gastrointest Cancer. 33: 103-6. -
Scholzen, T.E. et al. (2007) Terminating the stress: peripheral peptidolysis of proopiomelanocortin-derived regulatory hormones by the dermal microvascular endothelial cell extracellular peptidases neprilysin and angiotensin-converting enzyme.
Endocrinology. 148: 2793-805. -
de Moraes Schenka, N.G. et al. (2007) Use of p63 and CD10 in the differential diagnosis of papillary neoplasms of the breast.
Breast J. 14: 68-75. -
Matsumoto, T. et al. (2005) Increase of bone marrow-derived secretory lineage epithelial cells during regeneration in the human intestine.
Gastroenterology. 128: 1851-67. -
Rasini, V. et al. (2013) Mesenchymal stromal/stem cells markers in the human bone marrow.
Cytotherapy. 15 (3): 292-306. -
Nishimoto-Kakiuchi, A. et al. (2016) Characteristics of histologically confirmed endometriosis in cynomolgus monkeys.
Hum Reprod. Sep 2. [Epub ahead of print] -
Tasaki K et al. (2007) CD5-positive mucosa-associated lymphoid tissue (MALT) lymphoma of ocular adnexal origin: usefulness of fluorescence in situ hybridization for distinction between mantle cell lymphoma and MALT lymphoma.
Pathol Int. 57 (2): 101-7. -
Mizowaki, T. et al. (2015) STAT3 activation is associated with cerebrospinal fluid interleukin-10 (IL-10) in primary central nervous system diffuse large B cell lymphoma.
J Neurooncol. 124 (2): 165-74. -
Ince, T.A. et al. (2007) Transformation of different human breast epithelial cell types leads to distinct tumor phenotypes.
Cancer Cell. 12 (2): 160-70.
