CD10 antibody | 56C6

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Mouse anti Human CD10

Product Type
Monoclonal Antibody
Clone
56C6
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1806T C P* 0.1 ml
Mouse anti Human CD10 antibody, clone 56C6 recognizes human Neprilysin, also known as CD10, Atriopeptidase, Common acute lymphocytic leukemia antigen (CALLA), Enkephalinase, Neutral endopeptidase 24.11, Neutral endopeptidase or Skin fibroblast elastase. CD10 is a 749 amino acid, ~100 kDa single pass type II transmembrane glycoprotein, expressed on a variety of cell types including immature and germinal centre B cells, lymphoblastic leukaemia cells and also on the brush border of kidney epithelial cells.

Mutations in the MME gene (encoding CD10) are involved in the development of Charcot-Marie-Tooth disease 2T (CMT2T), a disorder of the peripheral nervous system, demonstrating progerssive peripheral muscle weakness and atrophy (Higuchi et al. 2016). Mutations of the MME gene can also lead to Spinocerebellar ataxia 43 (SCA43) characterized by a progressive loss of gait coordination along with hand, speech and eye coordination. Degeneration of the cerebellum, with variable involvement of the brain stem and spinal cord are apparent in this slow progressive autosomal dominant form of the disease (Depondt et al. 2016).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant human CD10.
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P08473 Related reagents
Entrez Gene
MME Related reagents
GO Terms
GO:0005515 protein binding
GO:0004222 metalloendopeptidase activity
GO:0005887 integral to plasma membrane
GO:0046872 metal ion binding
GO:0006508 proteolysis
GO:0007267 cell-cell signaling
Regulatory
For research purposes only

Applications of CD10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/80
Immunohistology - Paraffin 1 1/80
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Tonsil, Intestine or kidney

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for CD10 antibody

  1. Angelin-Duclos, C. et al. (2000) Commitment of B lymphocytes to a plasma cell fate is associated with Blimp-1 expression in vivo.
    J Immunol. 165 (10): 5462-71.
  2. Carvounis, E.E. et al. (2003) Undifferentiated carcinoma with osteoclast-like giant cells of the pancreas.
    Int J Gastrointest Cancer. 33: 103-6.
  3. Scholzen, T.E. et al. (2007) Terminating the stress: peripheral peptidolysis of proopiomelanocortin-derived regulatory hormones by the dermal microvascular endothelial cell extracellular peptidases neprilysin and angiotensin-converting enzyme.
    Endocrinology. 148: 2793-805.
  4. de Moraes Schenka, N.G. et al. (2007) Use of p63 and CD10 in the differential diagnosis of papillary neoplasms of the breast.
    Breast J. 14: 68-75.
  5. Matsumoto, T. et al. (2005) Increase of bone marrow-derived secretory lineage epithelial cells during regeneration in the human intestine.
    Gastroenterology. 128: 1851-67.
  6. Rasini, V. et al. (2013) Mesenchymal stromal/stem cells markers in the human bone marrow.
    Cytotherapy. 15 (3): 292-306.
  7. Nishimoto-Kakiuchi, A. et al. (2016) Characteristics of histologically confirmed endometriosis in cynomolgus monkeys.
    Hum Reprod. Sep 2. [Epub ahead of print]
  8. Tasaki K et al. (2007) CD5-positive mucosa-associated lymphoid tissue (MALT) lymphoma of ocular adnexal origin: usefulness of fluorescence in situ hybridization for distinction between mantle cell lymphoma and MALT lymphoma.
    Pathol Int. 57 (2): 101-7.
  9. Mizowaki, T. et al. (2015) STAT3 activation is associated with cerebrospinal fluid interleukin-10 (IL-10) in primary central nervous system diffuse large B cell lymphoma.
    J Neurooncol. 124 (2): 165-74.
  10. Ince, T.A. et al. (2007) Transformation of different human breast epithelial cell types leads to distinct tumor phenotypes.
    Cancer Cell. 12 (2): 160-70.