CCL8 antibody | E07-8G5

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Mouse anti Human CCL8

Product Type
Monoclonal Antibody
Clone
E07-8G5
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA6266GA
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human CCL8 antibody, clone E07-8G5, recognizes C-C motif chemokine-8 (CCL8) also known as HC14, monocyte chemoattractant protein 2, monocytes chemotactic protein 2 and small-inducible cytokine A8. CCL8 is a member of the C-C chemokine subfamily and is an agonist for C-C chemokine receptor type 2 and CCR5 (Ge et al. 2017). Through these receptors, CCL8 is involved in the immune response by attracting monocytes, T-lymphocytes, natural killer cells, basophils, mast cells and eosinophils (Farmaki et al. 2020). Proteolytic cleavage of CCL8 to CCL8 (6-76) converts the chemokine into a potent inhibitor of C-C chemokine-induced chemotaxis (Proost et al. 1998).

The purified Mouse anti Human CCL8 antibody, clone E07-8G5 (MCA6266GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human CCL8 antibody, clone E09-8A1 (MCA6267B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
E. coli derived recombinant human full length CCL8 (Gln24-Pro99)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P80075
Entrez Gene
CCL8
GO Terms
GO:0009615 response to virus
GO:0004871 signal transducer activity
GO:0005615 extracellular space
GO:0006816 calcium ion transport
GO:0006887 exocytosis
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0008009 chemokine activity
GO:0008201 heparin binding
Regulatory
For research purposes only

Applications of CCL8 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human CCL8:Biotin MCA6267B E 0.1 mg loader
Recombinant Human CCL8 PHP297 E 10 µg loader

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