CCL11 antibody | 1.3_17H09-02C06
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Mouse anti Human CCL11:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 1.3_17H09-02C06
- Isotype
- IgG1
- Specificity
- CCL11
Antibody Quality and Performance Guarantee
| Mouse anti Human CCL11 antibody, clone 1.3_17H09-02C06, recognizes C-C motif chemokine 11 (CCL11), also known as eotaxin, eosinophil chemotactic protein and small inducible cytokine A11. CCL11 is produced by a range of cell types including epithelial cells, fibroblasts, smooth muscle cells, astrocytes, chondrocytes and tissue resident macrophages. Additionally, in the central nervous system, it is expressed by choroid plexus epithelial cells, pericytes, and microglia under inflammatory conditions (Teixeira et al. 2018). CCL11 acts as a chemoattractant for eosinophil recruitment to inflammatory sites, including during asthma attacks (Wen & Rothenberg 2016). Upon binding of CCL11 to its receptor CCR3, a series of signaling events are triggered which can result in calcium mobilization, CD11b upregulation, MAPK activation and actin polymerization. It can also result in a rapid change of shape associated with chemotaxis and granule release (Conroy & Williams 2001). |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- E. coli derived recombinant human CCL11
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
- Endotoxin Level
- < 1 EU/μg
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
2.0 ug/ml | 1.0 ug/ml |
| Flow Cytometry | |
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| Western Blotting | |
0.25 ug/ml | 0.5 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
- Synonyms
- Eotaxin
- UniProt
- P51671
- Entrez Gene
- CCL11
- GO Terms
- GO:0001938 positive regulation of endothelial cell proliferation
- GO:0009615 response to virus
- GO:0007155 cell adhesion
- GO:0007165 signal transduction
- GO:0005615 extracellular space
- GO:0006468 protein phosphorylation
- GO:0006874 cellular calcium ion homeostasis
- GO:0006935 chemotaxis
- GO:0006954 inflammatory response
- View More GO Terms
- GO:0006955 immune response
- GO:0008009 chemokine activity
- GO:0009314 response to radiation
- GO:0030335 positive regulation of cell migration
- GO:0030838 positive regulation of actin filament polymerization
- GO:0032855 positive regulation of Rac GTPase activity
MCA6337Z
156491
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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