CA125 antibody | X75
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|Mouse anti Human CA125 antibody, clone X75 recognizes the ovarian cancer mucin CA125 (also known as MUCIN 16). CA125 consists of a short cytoplasmic tail, a transmembrane domain and an exceptionally large glycosylated extracellular domain. The extracellular domain is dominated by a large number of 156-amino acid repeat units, over 60 in all. These repeats are known to bind the antibodies OC125 and M-11.
CA125 is present within normal ovarian, endometrium, endocervix and fallopian tissue, but levels are elevated in over 90% of women with advanced ovarian cancer. CA125 levels may also be elevated in other cancers and non-cancerous conditions such as peritonitis, endometriosis and liver cirrhosis. Levels may also fluctuate during the menstrual cycle and occasionally increases in pregnancy, making CA125 an unreliable marker for ovarian cancer in premenopausal women. The function of CA125 in healthy tissue is unknown but it is thought to provide a protective lubricating barrier against particles and infectious agents at mucosal surfaces.
CA125 was concluded by the International Society of Oncology and BioMarkers (ISOBM) to contain two major antigenic domains. Mouse anti Human CA125 antibody, clone X75 recognizes the epitope specificity group B, similar to the M-11 antibody (Nustad et al. 2002). Mouse anti Human CA125 antibody, clone X75 has been used successfully as both a capture and detection reagent in the development of sensitive ELISA assays for the measurement of CA125 antigen in human plasma samples.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by ion exchange chromatography from ascites
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- CA125 antigen purified from human ovarian carcinoma.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
References for CA125 antibody
Nustad, K. et al. (2002) Epitopes on CA 125 from cervical mucus and ascites fluid and characterization of six new antibodies. Third report from the ISOBM TD-1 workshop.
Tumour Biol. 23: 303-14.
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