C3d antibody | 053A-514.3.1.4
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Mouse anti Human C3d antibody, clone 053A-514.3.1.4 recognizes human complement component 3d (C3d) neoantigen, a ~33 kDa polypeptide fragment generated over the course of complement activation in which where complement 3 (C3) convertases cleave C3 to C3b, which is further degraded into iC3b and C3dg/C3d.
C3d is involved in the regulation of many aspects of the immune response, including antigen processing and presentation. The C3d/antigen complex mediates B cell activation by simultaneously binding antigen-specific surface bound immunoglobulin and CD21, lowering the complement activation threshold. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.
C3d is involved in the regulation of many aspects of the immune response, including antigen processing and presentation. The C3d/antigen complex mediates B cell activation by simultaneously binding antigen-specific surface bound immunoglobulin and CD21, lowering the complement activation threshold. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide (NaN3) - Immunogen
- Purified human C3d (neoantigen).
- Approx. Protein Concentrations
- Current, batch-specific concentration 1.14 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P01024
- Entrez Gene
- C3
- GO Terms
- GO:0005102 receptor binding
- GO:0004866 endopeptidase inhibitor activity
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006957 complement activation, alternative pathway
- GO:0006958 complement activation, classical pathway
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0010575 positive regulation vascular endothelial growth factor production
- Regulatory
- For research purposes only
Applications of C3d antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry | |||
| Immunohistology - Frozen |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Kidney from post streptococcal glomerulonephritis patients
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for C3d antibody
-
Verghese PS et al. (2016) The clinical implications of the unique glomerular complement deposition pattern in transplant glomerulopathy.
J Nephrol. Nov 15. [Epub ahead of print] -
Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody.
US patent Application US20170315118A1
Further Reading
-
Bergmann-Leitner, E.S. et al. (2006) Complement 3d: from molecular adjuvant to target of immune escape mechanisms.
Clin Immunol. 121 (2): 177-85. -
Barilla-LaBarca, M.L. et al. (2002) Role of membrane cofactor protein (CD46) in regulation of C4b and C3b deposited on cells.
J Immunol. 168 (12): 6298-304.
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