C3d antibody | 053A-514.3.1.4

Mouse anti Human C3d

Product Type
Monoclonal Antibody
Clone
053A-514.3.1.4
Isotype
IgG1
Specificity
C3d

Product Code Applications Pack Size List Price Your Price Qty
MCA2648
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F 0.1 mg
List Price Your Price
Search for Batch Specific Datasheets

Mouse anti Human C3d antibody, clone 053A-514.3.1.4 recognizes human complement component 3d (C3d) neoantigen, a ~33 kDa polypeptide fragment generated over the course of complement activation in which where complement 3 (C3) convertases cleave C3 to C3b, which is further degraded into iC3b and C3dg/C3d.

C3d is involved in the regulation of many aspects of the immune response, including antigen processing and presentation. The C3d/antigen complex mediates B cell activation by simultaneously binding antigen-specific surface bound immunoglobulin and CD21, lowering the complement activation threshold. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
Immunogen
Purified human C3d (neoantigen).
Approx. Protein Concentrations
Current, batch-specific concentration 1.14 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Kidney from post streptococcal glomerulonephritis patients

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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control

References for C3d antibody

  1. Verghese PS et al. (2016) The clinical implications of the unique glomerular complement deposition pattern in transplant glomerulopathy.
    J Nephrol. Nov 15. [Epub ahead of print]
  2. Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody.
    US patent Application US20170315118A1

Further Reading

  1. Bergmann-Leitner, E.S. et al. (2006) Complement 3d: from molecular adjuvant to target of immune escape mechanisms.
    Clin Immunol. 121 (2): 177-85.
  2. Barilla-LaBarca, M.L. et al. (2002) Role of membrane cofactor protein (CD46) in regulation of C4b and C3b deposited on cells.
    J Immunol. 168 (12): 6298-304.

Synonyms
Complement Component 3d
RRID
AB_2259274
UniProt
P01024
Entrez Gene
C3
GO Terms
GO:0005102 receptor binding
GO:0004866 endopeptidase inhibitor activity
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006957 complement activation, alternative pathway
GO:0006958 complement activation, classical pathway
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0010575 positive regulation vascular endothelial growth factor production
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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