Mouse anti Human C3b antibody, clone H11 recognizes human C3b, part of the complement system. C3 is cleaved by C3-convertase creating C3a and C3b, which binds to the surface of pathogens leading to their destruction by phagocytic cells. Mouse anti Human C3b antibody, clone H11 recognizes a determinant present on native (circulating) C3 and on the fragments C3b and C3c. The antibody is directed to a determinant present on the betachain of the C3 molecule (Western blot). It does not react with the anaphylatoxin C3a or the fragment C3d and does not inhibit the haemolytic function of C3.
- Target Species
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gentle mixing of the vial following reconstitution is recommended.
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide, 0.5% Bovine Serum Albumin.
These concentrations relate to the reconstituted product.
- Human C3b.
- Approx. Protein Concentrations
- IgG concentration 50 ug/ml after reconstitution
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed until date of expiry. Please see product label.
- Entrez Gene
- GO Terms
endopeptidase inhibitor activity
complement activation, alternative pathway
complement activation, classical pathway
G-protein coupled receptor protein signaling pathway
positive regulation vascular endothelial growth factor production
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of C3b antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available