C3a antibody | 048-18.6.3.X (10-21)
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Mouse anti Human C3a antibody, clone 048-18.6.3.X (10-21) recognizes the ~9 kDa complement component 3a (C3a) which is present in the blood serum. C3a is generated over the course of complement activation where complement 3 (C3) convertases cleave C3 to C3a and C3b, which is further degraded into iC3b and C3dg/C3d. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.
C3a mediates local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3a is expressed at significantly higher levels in colorectal cancer.
Mouse anti Human C3a antibody, clone 10-21 shows minimal reactivity with other complement proteins in radioimmunoassays and haemagglutination.
Mouse anti Human C3a antibody, clone 10-21 has been reported to work in western blotting applications.
C3a mediates local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3a is expressed at significantly higher levels in colorectal cancer.
Mouse anti Human C3a antibody, clone 10-21 shows minimal reactivity with other complement proteins in radioimmunoassays and haemagglutination.
Mouse anti Human C3a antibody, clone 10-21 has been reported to work in western blotting applications.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Native purified human C3a.
- Approx. Protein Concentrations
- Current, batch-specific concentration 1.1 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P01024
- Entrez Gene
- C3
- GO Terms
- GO:0005102 receptor binding
- GO:0004866 endopeptidase inhibitor activity
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006957 complement activation, alternative pathway
- GO:0006958 complement activation, classical pathway
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0010575 positive regulation vascular endothelial growth factor production
- Regulatory
- For research purposes only
Applications of C3a antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/1500 | 1/6000 | |
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
Further Reading
-
Hugli, T.E. (1975) Human anaphylatoxin (C3a) from the third component of complement. Primary structure.
J Biol Chem. 250 (21): 8293-301. -
Habermann, J.K. et al. (2006) Increased serum levels of complement C3a anaphylatoxin indicate the presence of colorectal tumors.
Gastroenterology. 131 (4): 1020-9; quiz 1284.
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