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C1q antibody | 004-43.X (3R9/2)

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Mouse anti Human C1q

Product Type
Monoclonal Antibody
Clone
004-43.X (3R9/2)
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2603
Datasheet
C E F IF WB
MSDS
0.1 ml loader

Mouse anti human C1q antibody, clone 004-43.X (3R9/2), recognizes human complement component 1 q (C1q), a ~156 kDa secreted protein.
C1q associates with proenzymes C1r and C1s to form the calcium-dependent C1 complex, the first component of the serum complement system. C1q is composed of six A-, six B-chains and six C-polypeptide chains. Each chain contains a collagen-like region located near the N-terminus and a C-terminal globular region. These regions bind the Fc region of IgM and IgG molecules, initiating the classical pathway of complement activation.
C1q deficiency has been associated with lupus erythematosus and glomerulonephritis (Troedson et al. 2013).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Globular head domain of C1q, purified from human plasma.
Approx. Protein Concentrations
1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch

More Information

UniProt
P02745 Related reagents
P02746 Related reagents
P02747 Related reagents
Entrez Gene
C1QA Related reagents
C1QB Related reagents
C1QC Related reagents
GO Terms
GO:0005602 complement component C1 complex
GO:0006958 complement activation, classical pathway
GO:0007267 cell-cell signaling
GO:0045087 innate immune response
GO:0005576 extracellular region
GO:0030853 negative regulation of granulocyte differentiation
GO:0045650 negative regulation of macrophage differentiation
Regulatory
For research purposes only

Applications of C1q antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen 1:500 1:1000
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
For best results Bio-Rad recommend HISTAR detection kits.
Histology Positive Control Tissue
Kidney from patients with streptococcal glomerulonephritis
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for C1q antibody

  1. Gershov, D. et al. (2000) C-Reactive protein binds to apoptotic cells, protects the cells from assembly of the terminal complement components, and sustains an antiinflammatory innate immune response: implications for systemic autoimmunity.
    J Exp Med. 192 (9): 1353-64.
  2. Castellano, G. et al. (2010) Therapeutic targeting of classical and lectin pathways of complement protects from ischemia-reperfusion-induced renal damage.
    Am J Pathol. 176: 1648-59.
  3. Ma, W. et al. (2012) RAGE binds C1q and enhances C1q-mediated phagocytosis.
    Cell Immunol. 274: 72-82.
  4. Lachmann, N. et al. (2013) Systematic comparison of four cell- and Luminex-based methods for assessment of complement-activating HLA antibodies.
    Transplantation. 95 (5): 694-700.
  5. Cai, Y. et al. (2015) C1q protein binds to the apoptotic nucleolus and causes C1 protease degradation of nucleolar proteins.
    J Biol Chem. 290 (37): 22570-80.
  6. Madhukaran, S.P. et al. (2015) Decidual expression and localization of human surfactant protein SP-A and SP-D, and complement protein C1q.
    Mol Immunol. 66 (2): 197-207.
  7. Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody
    US Patent Application US20170315118A1
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