C1q antibody | 004-43.X (3R9/2)
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Mouse anti human C1q antibody, clone 004-43.X (3R9/2), recognizes human complement component 1 q (C1q), a ~156 kDa secreted protein.
C1q associates with proenzymes C1r and C1s to form the calcium-dependent C1 complex, the first component of the serum complement system. C1q is composed of six A-, six B-chains and six C-polypeptide chains. Each chain contains a collagen-like region located near the N-terminus and a C-terminal globular region. These regions bind the Fc region of IgM and IgG molecules, initiating the classical pathway of complement activation.
C1q deficiency has been associated with lupus erythematosus and glomerulonephritis (Troedson et al. 2013).
C1q associates with proenzymes C1r and C1s to form the calcium-dependent C1 complex, the first component of the serum complement system. C1q is composed of six A-, six B-chains and six C-polypeptide chains. Each chain contains a collagen-like region located near the N-terminus and a C-terminal globular region. These regions bind the Fc region of IgM and IgG molecules, initiating the classical pathway of complement activation.
C1q deficiency has been associated with lupus erythematosus and glomerulonephritis (Troedson et al. 2013).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Globular head domain of C1q, purified from human plasma.
- Approx. Protein Concentrations
- Current, batch-specific concentration 1.09 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P02745
- P02746
- P02747
- Entrez Gene
- C1QA
- C1QB
- C1QC
- GO Terms
- GO:0005602 complement component C1 complex
- GO:0006958 complement activation, classical pathway
- GO:0007267 cell-cell signaling
- GO:0045087 innate immune response
- GO:0005576 extracellular region
- GO:0030853 negative regulation of granulocyte differentiation
- GO:0045650 negative regulation of macrophage differentiation
- Regulatory
- For research purposes only
Applications of C1q antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | 1:500 | 1:1000 | |
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Kidney from patients with streptococcal glomerulonephritis
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for C1q antibody
-
Petry, F. (1998) Molecular basis of hereditary C1q deficiency.
Immunobiology. 199 (2): 286-94. -
Gershov, D. et al. (2000) C-Reactive protein binds to apoptotic cells, protects the cells from assembly of the terminal complement components, and sustains an antiinflammatory innate immune response: implications for systemic autoimmunity.
J Exp Med. 192 (9): 1353-64. -
Castellano, G. et al. (2010) Therapeutic targeting of classical and lectin pathways of complement protects from ischemia-reperfusion-induced renal damage.
Am J Pathol. 176: 1648-59. -
Ma, W. et al. (2012) RAGE binds C1q and enhances C1q-mediated phagocytosis.
Cell Immunol. 274: 72-82. -
Lachmann, N. et al. (2013) Systematic comparison of four cell- and Luminex-based methods for assessment of complement-activating HLA antibodies.
Transplantation. 95 (5): 694-700. -
Cai, Y. et al. (2015) C1q protein binds to the apoptotic nucleolus and causes C1 protease degradation of nucleolar proteins.
J Biol Chem. 290 (37): 22570-80. -
Madhukaran, S.P. et al. (2015) Decidual expression and localization of human surfactant protein SP-A and SP-D, and complement protein C1q.
Mol Immunol. 66 (2): 197-207. -
Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody
US Patent Application US20170315118A1
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