BRCA1 antibody | MS110

Mouse anti Human BRCA1 (N-Terminal)

Product Type
Monoclonal Antibody
Clone
MS110
Isotype
IgG1
Specificity
BRCA1
Region
(N-TERMINAL)

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MCA1682GT
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C * F IF IP P * WB 50 µg loader
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Mouse anti Human BRCA1 antibody, clone MS110 recognizes an epitope within the 304 amino acid N-Terminal (NT) region of human BRCA1, otherwise known as Breast cancer type 1 susceptibility protein, a tumor suppressor gene and major player in DNA damage repair, predominantly expressed in the nucleus during the S/G2 phase of the cell cycle.

Deleterious BRCA1 mutations corralate with increased risk of cancers in both males and females including breast, pancreatic and prostate cancer, although in males pancreatic and prostate cancer appear to be more strongly associated with BRCA2 gene mutations.

BRCA1 is a key marker of triple-negative breast cancer/TNBC (ER-/PR-/HER2-), a high risk aggressive cancer which makes up about 15% of invasive breast cancers, and which lacks the benefit of specific therapy that targets the three major proteins ER/PR/HER2. Triple-negative tumors are predominantly basal-like, poorly differentiated and of higher histological grade. Younger women have an increased rate of basal or BRCA related TNBC, compared with the higher proportion of apocrine, normal-like and rare subtypes of TNBC, seen in older women.

Mouse anti human BRCA1 antibody, clone MS110 is suitable for use in both immunohistochemical and immunofluorescence staining of human breast and ovarian tissue, and clone MS110 is widely used and reported (Milner et al.. 2013).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
TRIS-glycine buffered saline, NaCl
Preservative Stabilisers
<0.1% sodium azide (NaN3)
Immunogen
Recombinant protein corresponding to the N-Terminal region of human BRCA1.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse NSI myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen 1
Immunohistology - Paraffin 2
Immunoprecipitation
Western Blotting 1.0ug/ml
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of frozen sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. See Yoshikawa, K.et al. for details.
  2. 2 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. See Yoshikawa, K.et al. for details.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human breast carcinoma
Western Blotting
Mouse anti Human BRCA1 antibody, clone MS110 detects a band of approximately 220kDa in HeLa nuclear extract. A smaller band of 85kDa may also be seen, which may represent post-translational modification.

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References for BRCA1 antibody

  1. Scully, R. et al. (1996) Location of BRCA1 in human breast and ovarian cancer cells.
    Science. 272 (5258): 123-6.
  2. Yoshikawa, K. et al. (1999) Reduction of BRCA1 protein expression in Japanese sporadic breast carcinomas and its frequent loss in BRCA1-associated cases.
    Clin Cancer Res. 5 (6): 1249-61.
  3. Scully, R. et al. (1997) Association of BRCA1 with Rad51 in mitotic and meiotic cells.
    Cell. 88: 265-75.
  4. Chen, J. (2000) Ataxia telangiectasia-related protein is involved in the phosphorylation of BRCA1 following deoxyribonucleic acid damage.
    Cancer Res. 60: 5037-9.
  5. Fraser, J.A. et al. (2003) A role for BRCA1 in sporadic breast cancer.
    Br J Cancer. 88: 1263-70.
  6. Kleiman, F.E. et al. (2005) BRCA1/BARD1 inhibition of mRNA 3' processing involves targeted degradation of RNA polymerase II.
    Genes Dev. 19: 1227-37.
  7. Pageau, G.J. and Lawrence, J.B. (2006) BRCA1 foci in normal S-phase nuclei are linked to interphase centromeres and replication of pericentric heterochromatin.
    J Cell Biol. 175: 693-701.

Further Reading

  1. Milner, R. et al. (2013) Validation of the BRCA1 antibody MS110 and the utility of BRCA1 as a patient selection biomarker in immunohistochemical analysis of breast and ovarian tumours.
    Virchows Arch. 462 (3): 269-79.

Immunocytochemistry

Immunofluorescence

Immunohistology - Frozen

Western Blotting

Synonyms
Breast Cancer Type 1 Susceptibility Protein
UniProt
P38398
Entrez Gene
BRCA1
GO Terms
GO:0007059 chromosome segregation
GO:0005515 protein binding
GO:0000724 double-strand break repair via homologous recombination
GO:0003677 DNA binding
GO:0003713 transcription coactivator activity
GO:0003723 RNA binding
GO:0030529 ribonucleoprotein complex
GO:0006915 apoptosis
GO:0004842 ubiquitin-protein ligase activity
GO:0005654 nucleoplasm
GO:0006301 postreplication repair
GO:0006359 regulation of transcription from RNA polymerase III promoter
GO:0006633 fatty acid biosynthetic process
GO:0006978 DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator
GO:0007049 cell cycle
GO:0008270 zinc ion binding
GO:0008274 gamma-tubulin ring complex
GO:0008630 DNA damage response, signal transduction resulting in induction of apoptosis
GO:0042802 identical protein binding
GO:0010212 response to ionizing radiation
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0015631 tubulin binding
GO:0016481 negative regulation of transcription
GO:0016563 transcription activator activity
GO:0030521 androgen receptor signaling pathway
GO:0031398 positive regulation of protein ubiquitination
GO:0031436 BRCA1-BARD1 complex
GO:0031572 G2/M transition DNA damage checkpoint
GO:0031625 ubiquitin protein ligase binding
GO:0042127 regulation of cell proliferation
GO:0043627 response to estrogen stimulus
GO:0045717 negative regulation of fatty acid biosynthetic process
GO:0045739 positive regulation of DNA repair
GO:0046600 negative regulation of centriole replication
GO:0050681 androgen receptor binding
GO:0051865 protein autoubiquitination
GO:0070531 BRCA1-A complex
GO:0071681 cellular response to indole-3-methanol
GO:0085020 protein K6-linked ubiquitination
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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