BLTR antibody | 202/7B1
Mouse anti Human BLTR antibody, clone 202/7B1 (MCA2108) used for the detection of Leukotriene B4 receptor 1 expressing cultured peripheral blood mononuclear cells by immunofluorescence.
Image caption:
Leukotriene B4 biosynthetic enzymes and receptors are expressed in both macrophages and osteoclasts. (a) Quantification of leukotriene B4 (LTB4) levels by enzyme-linked immunoassay in the conditioned medium of human peripheral blood mononuclear cells (PBMCs) treated with 25 ng/ml macrophage colony-stimulating factor (M-CSF) and 10 ng/ml interleukin 23 (IL-23) for 3 days. Representative data from three experiments are shown. **P < 0.01. (b) Immunofluorescence of human PBMCs cultured with either M-CSF alone for 8 days or M-CSF + receptor activator of nuclear factor κB ligand (RANKL) for 14 days showing the expression of BLT1, BLT2, 5-lipoxygenase (5-LO) and leukotriene A4 hydrolase (LTA4H) in mononuclear and multinucleated giant cells. Representative images from three experiments are shown. 4′,6-diamidino-2-phenylindole (DAPI) is blue and BLT1, BLT2, 5-LO and LTA4H are in red. The scale bars represent 20 μm in Figure 1b.
From: Dixit N, Wu DJ, Belgacem YH, Borodinsky LN, Gershwin M, Adamopoulos IE.
Leukotriene B4 activates intracellular calcium and augments human osteoclastogenesis.
Arthritis Res Ther. 2014 Dec 2;16(6):496.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
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F IF ResetMouse anti Human BLTR
- Product Type
- Monoclonal Antibody
- Clone
- 202/7B1
- Isotype
- IgG2a
- Specificity
- BLTR
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Antibody Quality and 
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| Mouse anti Human BLTR antibody, clone 202/7B1 recognizes Human leukotriene B4 receptor, also known as BLTR, Chemoattractant receptor-like 1, G-protein coupled receptor 16 or P2Y purinoceptor 7. BLTR is a 353 ~40 kDa multipass transmembrane glycoprotein BLTR is expressed by peripheral blood granulocytes and is important in the pro-inflammatory response. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- MCA2108GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- MCA2108: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Pack Size: 0.1 mg
0.09% Sodium Azide Pack Size: 0.2 mg0.09% sodium azide. - Carrier Free
- Yes
- Immunogen
- HeLa cell transfectants expressing human BLTR.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 Pack Size: 0.1 mg 1/50 Pack Size: 0.2 mg |
1/200 Pack Size: 0.1 mg 1/100 Pack Size: 0.2 mg |
| Immunoassay | |
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| Immunofluorescence | |
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| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
References for BLTR antibody
-
Pettersson, A. et al. (2000) First-generation monoclonal antibodies identifying the human leukotriene B(4) receptor-1.
Biochem Biophys Res Commun. 279 (2): 520-5. -
Costa, M.F. et al. (2010) Leukotriene B4 mediates gammadelta T lymphocyte migration in response to diverse stimuli.
J Leukoc Biol. 87: 323-32. -
Islam, S.A. et al. (2006) The leukotriene B4 lipid chemoattractant receptor BLT1 defines antigen-primed T cells in humans.
Blood. 107: 444-53. -
Medoff, B.D. et al. (2005) BLT1-mediated T cell trafficking is critical for rejection and obliterative bronchiolitis after lung transplantation.
J Exp Med. 202: 97-110. -
Serezani, C.H. et al. (2009) FcgammaRI ligation leads to a complex with BLT1 in lipid rafts that enhances rat lung macrophage antimicrobial functions.
Blood. 114: 3316-24. -
Heller, E.A. et al. (2005) Inhibition of atherogenesis in BLT1-deficient mice reveals a role for LTB4 and BLT1 in smooth muscle cell recruitment.
Circulation. 112: 578-86. -
Thomas, S.Y. et al. (2007) Multiple chemokine receptors, including CCR6 and CXCR3, regulate antigen-induced T cell homing to the human asthmatic airway.
J Immunol. 179: 1901-12. -
Sabirsh, A. et al. (2005) Fluorescent leukotriene B4: potential applications.
J Lipid Res. 46: 1339-46.
View The Latest Product References
-
Loell, I. et al. (2012) Activated LTB4 pathway in muscle tissue of patients with polymyositis or dermatomyositis.
Ann Rheum Dis. 72: 293-9. -
Wang, L. et al. (2012) BLT1-Dependent Alveolar Recruitment of CD4+CD25+ Foxp3+ Regulatory T Cells is Important for Resolution of Acute Lung Injury.
Am J Respir Crit Care Med.186: 989-98. -
Laarman, A.J. et al. (2012) Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis.
EMBO J. 31: 3607-19. -
Dixit, N. et al. (2014) Leukotriene B4 activates intracellular calcium and augments human osteoclastogenesis.
Arthritis Res Ther. 16: 496. -
Sezin, T. et al. (2019) Dual inhibition of complement factor 5 and leukotriene B4 synergistically suppresses murine pemphigoid disease.
JCI Insight. 4 (15)Aug 08 [Epub ahead of print]. -
Askarian, F. et al. (2021) The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa. virulence in systemic infection.
Nat Commun. 12 (1): 1230. -
Williams, K.M. et al. (2022) Prospective Phase II Trial of Montelukast to Treat Bronchiolitis Obliterans Syndrome after Hematopoietic Cell Transplantation and Investigation into Bronchiolitis Obliterans Syndrome Pathogenesis.
Transplant Cell Ther. S2666-6367(22)00049-5.
- Synonyms
- Leukotriene B4 Receptor
- RRID
- AB_323834
- UniProt
- Q15722
- Entrez Gene
- LTB4R
- GO Terms
- GO:0000166 nucleotide binding
- GO:0006936 muscle contraction
- GO:0005887 integral to plasma membrane
- GO:0006928 cellular component movement
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0007200 activation of phospholipase C activity by G-protein coupled receptor protein signaling pathway coupled to IP3 second messenger
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