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BLTR antibody | 202/7B1

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Anti Human BLTR Antibody, clone 202/7B1 thumbnail image 1 Anti Human BLTR Antibody, clone 202/7B1 thumbnail image 2 Anti Human BLTR Antibody, clone 202/7B1 thumbnail image 3 Anti Human BLTR Antibody, clone 202/7B1 thumbnail image 4 Anti Human BLTR Antibody, clone 202/7B1 thumbnail image 5 Anti Human BLTR Antibody, clone 202/7B1 thumbnail image 6
Anti Human BLTR Antibody, clone 202/7B1 gallery image 1
Staining of human peripheral blood granulocytes with Mouse anti Human BLTR:FITC (MCA2108F).
Anti Human BLTR Antibody, clone 202/7B1 gallery image 2
Staining of human peripheral blood granulocytes with Mouse anti Human BLTR:Alexa Fluor® 647 (MCA2108A647).
Anti Human BLTR Antibody, clone 202/7B1 gallery image 3
Staining of human peripheral blood granulocytes with Mouse anti Human BLTR:RPE (MCA2108PE).
Anti Human BLTR Antibody, clone 202/7B1 gallery image 4
Staining of human peripheral blood monocytes with Mouse anti Human BLTR (MCA2108GA).
Anti Human BLTR Antibody, clone 202/7B1 gallery image 5
Staining of human peripheral blood monocytes with preservative free Mouse anti Human BLTR (MCA2108XZ).
Anti Human BLTR Antibody, clone 202/7B1 gallery image 6
Published customer image:
Mouse anti Human BLTR antibody, clone 202/7B1 (MCA2108) used for the detection of Leukotriene B4 receptor 1 expressing cultured peripheral blood mononuclear cells by immunofluorescence.
Image caption:
Leukotriene B4 biosynthetic enzymes and receptors are expressed in both macrophages and osteoclasts. (a) Quantification of leukotriene B4 (LTB4) levels by enzyme-linked immunoassay in the conditioned medium of human peripheral blood mononuclear cells (PBMCs) treated with 25 ng/ml macrophage colony-stimulating factor (M-CSF) and 10 ng/ml interleukin 23 (IL-23) for 3 days. Representative data from three experiments are shown. **P < 0.01. (b) Immunofluorescence of human PBMCs cultured with either M-CSF alone for 8 days or M-CSF + receptor activator of nuclear factor κB ligand (RANKL) for 14 days showing the expression of BLT1, BLT2, 5-lipoxygenase (5-LO) and leukotriene A4 hydrolase (LTA4H) in mononuclear and multinucleated giant cells. Representative images from three experiments are shown. 4′,6-diamidino-2-phenylindole (DAPI) is blue and BLT1, BLT2, 5-LO and LTA4H are in red. The scale bars represent 20 μm in Figure 1b.

From: Dixit N, Wu DJ, Belgacem YH, Borodinsky LN, Gershwin M, Adamopoulos IE.
Leukotriene B4 activates intracellular calcium and augments human osteoclastogenesis.
Arthritis Res Ther. 2014 Dec 2;16(6):496.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human BLTR:Alexa Fluor® 647

Mouse anti Human BLTR:FITC

Mouse anti Human BLTR

Product Type
Monoclonal Antibody
Clone
202/7B1
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA2108A647
Datasheet
F
SDS
100 Tests/1ml loader

MCA2108FT
Datasheet
C F
SDS
25 µg loader

MCA2108F
Datasheet
C F
SDS
0.1 mg loader

MCA2108
Datasheet
C F IF IY
SDS
0.2 mg loader

MCA2108GA
Datasheet
C F IF IY
SDS
0.1 mg loader

Mouse anti Human BLTR antibody, clone 202/7B1 recognizes Human leukotriene B4 receptor, also known as BLTR, Chemoattractant receptor-like 1, G-protein coupled receptor 16 or P2Y purinoceptor 7. BLTR is a 353 ~40 kDa multipass transmembrane glycoprotein

BLTR is expressed by peripheral blood granulocytes and is important in the pro-inflammatory response.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor® 647- liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
Pack Size: 25 µg
0.09%
1%
Sodium Azide
Bovine Serum Albumin
Pack Size: 0.1 mg
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
Pack Size: 0.2 mg
0.09% sodium azide.
Pack Size: 0.1 mg
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
HeLa cell transfectants expressing human BLTR.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
Q15722
Entrez Gene
LTB4R
GO Terms
GO:0000166 nucleotide binding
GO:0006936 muscle contraction
GO:0005887 integral to plasma membrane
GO:0006928 cellular component movement
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007200 activation of phospholipase C activity by G-protein coupled receptor protein signaling pathway coupled to IP3 second messenger
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of BLTR antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/10
Flow Cytometry Neat
Immunohistology - Frozen
Flow Cytometry 1/50 Pack Size: 0.2 mg
1/100 Pack Size: 0.1 mg		 1/100 Pack Size: 0.2 mg
1/200 Pack Size: 0.1 mg
Immunoassay
Immunofluorescence
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positiv controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positiv controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control:Alexa Fluor® 647 MCA929A647 F 100 Tests/1ml loader
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
Mouse IgG2a Negative Control MCA929 F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Flow Cytometry

Immunoassay

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for BLTR antibody

  1. Pettersson, A. et al. (2000) First-generation monoclonal antibodies identifying the human leukotriene B(4) receptor-1.
    Biochem Biophys Res Commun. 279 (2): 520-5.
  2. Costa, M.F. et al. (2010) Leukotriene B4 mediates gammadelta T lymphocyte migration in response to diverse stimuli.
    J Leukoc Biol. 87: 323-32.
  3. Islam, S.A. et al. (2006) The leukotriene B4 lipid chemoattractant receptor BLT1 defines antigen-primed T cells in humans.
    Blood. 107: 444-53.
  4. Medoff, B.D. et al. (2005) BLT1-mediated T cell trafficking is critical for rejection and obliterative bronchiolitis after lung transplantation.
    J Exp Med. 202: 97-110.
  5. Serezani, C.H. et al. (2009) FcgammaRI ligation leads to a complex with BLT1 in lipid rafts that enhances rat lung macrophage antimicrobial functions.
    Blood. 114: 3316-24.
  6. Heller, E.A. et al. (2005) Inhibition of atherogenesis in BLT1-deficient mice reveals a role for LTB4 and BLT1 in smooth muscle cell recruitment.
    Circulation. 112: 578-86.
  7. Thomas, S.Y. et al. (2007) Multiple chemokine receptors, including CCR6 and CXCR3, regulate antigen-induced T cell homing to the human asthmatic airway.
    J Immunol. 179: 1901-12.
  8. Sabirsh, A. et al. (2005) Fluorescent leukotriene B4: potential applications.
    J Lipid Res. 46: 1339-46.
  9. Loell, I. et al. (2012) Activated LTB4 pathway in muscle tissue of patients with polymyositis or dermatomyositis.
    Ann Rheum Dis. 72: 293-9.
  10. Wang, L. et al. (2012) BLT1-Dependent Alveolar Recruitment of CD4+CD25+ Foxp3+ Regulatory T Cells is Important for Resolution of Acute Lung Injury.
    Am J Respir Crit Care Med.186: 989-98.
  11. Laarman, A.J. et al. (2012) Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis.
    EMBO J. 31: 3607-19.
  12. Dixit, N. et al. (2014) Leukotriene B4 activates intracellular calcium and augments human osteoclastogenesis.
    Arthritis Res Ther. 16: 496.
  13. Sezin, T. et al. (2019) Dual inhibition of complement factor 5 and leukotriene B4 synergistically suppresses murine pemphigoid disease.
    JCI Insight. 4 (15)Aug 08 [Epub ahead of print].
  14. Askarian, F. et al. (2021) The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa. virulence in systemic infection.
    Nat Commun. 12 (1): 1230.

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