BLTR antibody | 202/7B1
Mouse anti Human BLTR antibody, clone 202/7B1 used for the detection of Leukotriene B4 receptor 1 expressing cultured peripheral blood mononuclear cells by immunofluorescence.
Image caption:
Leukotriene B4 biosynthetic enzymes and receptors are expressed in both macrophages and osteoclasts. (a) Quantification of leukotriene B4 (LTB4) levels by enzyme-linked immunoassay in the conditioned medium of human peripheral blood mononuclear cells (PBMCs) treated with 25 ng/ml macrophage colony-stimulating factor (M-CSF) and 10 ng/ml interleukin 23 (IL-23) for 3 days. Representative data from three experiments are shown. **P < 0.01. (b) Immunofluorescence of human PBMCs cultured with either M-CSF alone for 8 days or M-CSF + receptor activator of nuclear factor κB ligand (RANKL) for 14 days showing the expression of BLT1, BLT2, 5-lipoxygenase (5-LO) and leukotriene A4 hydrolase (LTA4H) in mononuclear and multinucleated giant cells. Representative images from three experiments are shown. 4′,6-diamidino-2-phenylindole (DAPI) is blue and BLT1, BLT2, 5-LO and LTA4H are in red. The scale bars represent 20 μm in Figure 1b.
From:Dixit N, Wu DJ, Belgacem YH, Borodinsky LN, Gershwin M, Adamopoulos IE. Leukotriene B4 activates intracellular calcium and augments human osteoclastogenesis. Arthritis Res Ther. 2014 Dec 2;16(6):496.
Mouse anti Human BLTR:Alexa Fluor® 647
Mouse anti Human BLTR:Preservative Free
Mouse anti Human BLTR:FITC
Mouse anti Human BLTR
Mouse anti Human BLTR:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 202/7B1
- Isotype
- IgG2a
Mouse anti Human BLTR antibody, clone 202/7B1 recognizes Human leukotriene B4 receptor, also known as BLTR, Chemoattractant receptor-like 1, G-protein coupled receptor 16 or P2Y purinoceptor 7. BLTR is a 353 ~40 kDa multipass transmembrane glycoprotein
BLTR is expressed by peripheral blood granulocytes and is important in the pro-inflammatory response.
BLTR is expressed by peripheral blood granulocytes and is important in the pro-inflammatory response.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647- liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Pack Size: 100 TestsReconstitute with 1 ml distilled waterPack Size: 25 TestsReconstitute in 0.25 ml disilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Pack Size: 25 Tests/0.25ml0.09%
1%
Sodium Azide
Bovine Serum AlbuminPack Size: 100 Tests/1ml0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
- 0.09%
1%
Sodium Azide
Bovine Serum Albumin - Preservative Stabilisers
- Pack Size: 0.2 mg0.09% sodium azide.Pack Size: 0.1 mg
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- HeLa cell transfectants expressing human BLTR.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- Q15722 Related reagents
- Entrez Gene
- LTB4R Related reagents
- GO Terms
- GO:0000166 nucleotide binding
- GO:0006936 muscle contraction
- GO:0005887 integral to plasma membrane
- GO:0006928 cellular component movement
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0007200 activation of phospholipase C activity by G-protein coupled receptor protein signaling pathway coupled to IP3 second messenger
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of BLTR antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/5 | 1/10 | |
| Flow Cytometry | 1/100 | 1/200 | |
| Immunoassay | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Flow Cytometry | Neat | ||
| Immunohistology - Frozen | |||
| Flow Cytometry | 1/50 Pack Size: 0.2 mg 1/100 Pack Size: 0.1 mg |
1/100 Pack Size: 0.2 mg 1/200 Pack Size: 0.1 mg |
|
| Immunoassay | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Flow Cytometry | Neat | 1/2 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positiv controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive control.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positiv controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive control.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:Alexa Fluor® 647 | MCA929A647 | 100 Tests/1ml | F | ||
| Mouse IgG2a Negative Control | MCA929 | 100 Tests | F | ||
| Mouse IgG2a Negative Control:FITC | MCA929F | 100 Tests | F | ||
| Mouse IgG2a Negative Control | MCA929 | 100 Tests | F | ||
| Mouse IgG2a Negative Control:RPE | MCA929PE | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F |
Application Based External Images
Flow Cytometry
Immunoassay
Immunofluorescence
Immunohistology - Frozen
Product Specific References
References for BLTR antibody
-
Pettersson, A. et al. (2000) First-generation monoclonal antibodies identifying the human leukotriene B(4) receptor-1.
Biochem Biophys Res Commun. 279 (2): 520-5. -
Costa, M.F. et al. (2010) Leukotriene B4 mediates gammadelta T lymphocyte migration in response to diverse stimuli.
J Leukoc Biol. 87: 323-32. -
Islam, S.A. et al. (2006) The leukotriene B4 lipid chemoattractant receptor BLT1 defines antigen-primed T cells in humans.
Blood. 107: 444-53. -
Medoff, B.D. et al. (2005) BLT1-mediated T cell trafficking is critical for rejection and obliterative bronchiolitis after lung transplantation.
J Exp Med. 202: 97-110. -
Serezani, C.H. et al. (2009) FcgammaRI ligation leads to a complex with BLT1 in lipid rafts that enhances rat lung macrophage antimicrobial functions.
Blood. 114: 3316-24. -
Heller, E.A. et al. (2005) Inhibition of atherogenesis in BLT1-deficient mice reveals a role for LTB4 and BLT1 in smooth muscle cell recruitment.
Circulation. 112: 578-86. -
Thomas, S.Y. et al. (2007) Multiple chemokine receptors, including CCR6 and CXCR3, regulate antigen-induced T cell homing to the human asthmatic airway.
J Immunol. 179: 1901-12. -
Sabirsh, A. et al. (2005) Fluorescent leukotriene B4: potential applications.
J Lipid Res. 46: 1339-46. -
Loell, I. et al. (2012) Activated LTB4 pathway in muscle tissue of patients with polymyositis or dermatomyositis.
Ann Rheum Dis. 72: 293-9. -
Wang, L. et al. (2012) BLT1-Dependent Alveolar Recruitment of CD4+CD25+ Foxp3+ Regulatory T Cells is Important for Resolution of Acute Lung Injury.
Am J Respir Crit Care Med.186: 989-98. -
Laarman, A.J. et al. (2012) Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis.
EMBO J. 31: 3607-19. -
Dixit, N. et al. (2014) Leukotriene B4 activates intracellular calcium and augments human osteoclastogenesis.
Arthritis Res Ther. 16: 496.
