Aurora-A Kinase antibody | 35C1







Aurora-A kinase is reported to be overexpressed in many epithelial cancers and is thought to play an important role in tumorigenesis (Katayama et al. 2003). Aurora A kinase appears to facilitate phosphorylation of centrin and co-localizes with it at centrosomes with maximum expression through prophase to late metaphase (Lukasiewicz et al. 2011).
Mouse anti human Aurora-A kinase, clone 35C1 recognizes an epitope within the non-catalytic N-terminal domain of Aurora-A. Clone 35C1 does not inhibit Aurora-A kinase activity (Cremet et al. 2003).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Recombinant Aurora-A
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c were fused with cells of the SP2/0-Ag14 mouse myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O14965
- Entrez Gene
- AURKA
- GO Terms
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0005524 ATP binding
- GO:0005634 nucleus
- GO:0005876 spindle microtubule
- GO:0006468 protein phosphorylation
- GO:0007051 spindle organization
- GO:0007067 mitosis
- GO:0019901 protein kinase binding
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
- GO:0031616 spindle pole centrosome
- GO:0031647 regulation of protein stability
- GO:0048471 perinuclear region of cytoplasm
- GO:0051301 cell division
- Regulatory
- For research purposes only
Applications of Aurora-A Kinase antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunofluorescence | |||
Immunoprecipitation | |||
Western Blotting | 1/500 | 1/1000 |
- Histology Positive Control Tissue
- Human 293 and mouse LLC1 cell lines.
- Western Blotting
- MCA2249 detects a band of approximately 46kDa in human HeLa and mouse M-ICc12 cell lysates.
Secondary Antibodies Available
Product Specific References
References for Aurora-A Kinase antibody
-
Cremet, J.Y. et al. (2003) Preparation and characterization of a human aurora-A kinase monoclonal antibody.
Mol Cell Biochem. 243 (1-2): 123-31. -
Hartsink-Segers SA et al. (2013) Inhibiting Polo-like kinase 1 causes growth reduction and apoptosis in pediatric acute lymphoblastic leukemia cells.
Haematologica. 98 (10): 1539-46. -
Hartsink-Segers, S.A. et al. (2013) Aurora kinases in childhood acute leukemia: the promise of aurora B as therapeutic target.
Leukemia. 27: 560-8. -
Lukasiewicz, K.B. et al. (2011) Control of centrin stability by Aurora A.
PLoS One. 6: e21291. -
Douglas, P. et al. (2015) Phosphorylation of SAF-A/hnRNP-U Serine 59 by Polo-Like Kinase 1 Is Required for Mitosis.
Mol Cell Biol. 35 (15): 2699-713.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra