Aurora-A Kinase antibody | 35C1

Mouse anti Aurora-A Kinase

Product Type
Monoclonal Antibody
Clone
35C1
Isotype
IgG2b
Specificity
Aurora-A Kinase

Product Code Applications Pack Size List Price Your Price Qty
MCA2249
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF IP WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anri Human Aurora -A kinase monoclonal antibody, clone 35C1 recognizes human Aurora-A kinase also known as Aurora 2, breast tumor-amplified kinase and serine/threonine-protein kinase 6 or 15. Aurora kinase A is member of the Ser/Thr protein kinase family containing a single protein kinase domain, has a molecular weight of ~46kDa and is involved in mitotic spindle assembly (Ducat et al. 2004).

Aurora-A kinase is reported to be overexpressed in many epithelial cancers and is thought to play an important role in tumorigenesis (Katayama et al. 2003). Aurora A kinase appears to facilitate phosphorylation of centrin and co-localizes with it at centrosomes with maximum expression through prophase to late metaphase (Lukasiewicz et al. 2011).

Mouse anti human Aurora-A kinase, clone 35C1 recognizes an epitope within the non-catalytic N-terminal domain of Aurora-A. Clone 35C1 does not inhibit Aurora-A kinase activity (Cremet et al. 2003).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Recombinant Aurora-A
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c were fused with cells of the SP2/0-Ag14 mouse myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunoprecipitation
Western Blotting 1/500 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
Histology Positive Control Tissue
Human 293 and mouse LLC1 cell lines.
Western Blotting
MCA2249 detects a band of approximately 46kDa in human HeLa and mouse M-ICc12 cell lysates.

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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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References for Aurora-A Kinase antibody

  1. Cremet, J.Y. et al. (2003) Preparation and characterization of a human aurora-A kinase monoclonal antibody.
    Mol Cell Biochem. 243 (1-2): 123-31.
  2. Hartsink-Segers SA et al. (2013) Inhibiting Polo-like kinase 1 causes growth reduction and apoptosis in pediatric acute lymphoblastic leukemia cells.
    Haematologica. 98 (10): 1539-46.
  3. Hartsink-Segers, S.A. et al. (2013) Aurora kinases in childhood acute leukemia: the promise of aurora B as therapeutic target.
    Leukemia. 27: 560-8.
  4. Lukasiewicz, K.B. et al. (2011) Control of centrin stability by Aurora A.
    PLoS One. 6: e21291.
  5. Douglas, P. et al. (2015) Phosphorylation of SAF-A/hnRNP-U Serine 59 by Polo-Like Kinase 1 Is Required for Mitosis.
    Mol Cell Biol. 35 (15): 2699-713.

Immunoblotting

Western Blotting

Synonyms
Serine/Threonine Kinase 15
RRID
AB_567387
UniProt
O14965
Entrez Gene
AURKA
GO Terms
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005876 spindle microtubule
GO:0006468 protein phosphorylation
GO:0007051 spindle organization
GO:0007067 mitosis
GO:0019901 protein kinase binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0031616 spindle pole centrosome
GO:0031647 regulation of protein stability
GO:0048471 perinuclear region of cytoplasm
GO:0051301 cell division
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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