Apolipoprotein E antibody | WUE-4

100% Secure

Anti Human Apolipoprotein E Antibody, clone WUE-4 thumbnail image 1

Anti Human Apolipoprotein E Antibody, clone WUE-4 thumbnail image 2

Anti Human Apolipoprotein E Antibody, clone WUE-4 thumbnail image 3

Anti Human Apolipoprotein E Antibody, clone WUE-4 thumbnail image 4

Anti Human Apolipoprotein E Antibody, clone WUE-4 gallery image 1 — Human apolipoprotein E detected with Mouse anti Human apolipoprotein E:HRP (**MCA5639P**).

Anti Human Apolipoprotein E Antibody, clone WUE-4 gallery image 2 — Human apolipoprotein E detected with Mouse anti Human apolipoprotein E (**MCA5639**) followed by Rabbit F(ab')₂ anti Mouse IgG:HRP (STAR13B)

Anti Human Apolipoprotein E Antibody, clone WUE-4 gallery image 3 — Sandwich ELISA analysis of human apolipoprotein E using Mouse anti Human apolipoprotein E (**MCA5639**) as a capture reagnt and biotinylated Goat anti Human Apolipoprotein E (AHP2177) as a detection reagent with purified apolipoprotein E as antigen. Detection is with Streptavidin HRP. A serum sample 1/150 (green) and a plasma sample 1/150 (orange) are shown. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135).

Anti Human Apolipoprotein E Antibody, clone WUE-4 gallery image 4 — **Published investigator image:**
Mouse anti Human Apolipoprotein E antibody, clone WUE-4 (**MCA5639**) used for the evaluation of Apo-E expression in culture media by western blotting.
**Image caption:**
Effects of the apoE-derived peptides and the HSPG-binding peptide 6a-P on heparin binding.
Heparin-immobilized beads (Pierce) were pre-equilibrated with PBS and then incubated with 500 μl of Huh-7.5 cell culture medium in the absence or presence of varying concentrations of the peptide hEP or 6a-P. The mutant peptides hEPm and 6a-Pm were used as controls. After 2 hrs incubation at room temperature, apoE-bound heparin-immobilized beads were spun down by centrifugation. The supernatant was collected and used for detection of the unbound apoE. The apoE-bound beads in pellet were washed three times with 1 ml of PBS. The heparin-bound and unbound apoE proteins were measured by Western blotting using an apoE-specific monoclonal antibody (WuE4).

From: Jiang J, Wu X, Tang H, Luo G (2013)
Apolipoprotein E Mediates Attachment of Clinical Hepatitis C Virus to Hepatocytes by Binding to Cell Surface Heparan Sulfate Proteoglycan Receptors.
PLoS ONE 8(7): e67982.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human Apolipoprotein E:Biotin

Mouse anti Human Apolipoprotein E:HRP

Mouse anti Human Apolipoprotein E:Low Endotoxin

Mouse anti Human Apolipoprotein E

Product Type: Monoclonal Antibody
Clone: WUE-4
Isotype: IgG1

Product Code	Applications	Pack Size
MCA5639B	E WB	0.1 mg
MCA5639P	E WB	0.1 mg
MCA5639EL	E FN WB	0.5 mg
MCA5639GA	E WB	0.1 mg
MCA5639	E WB	0.2 mg

Mouse anti Human Apolipoprotein E antibody, clone WUE-4 recognizes an epitope within amino acids 140-160 of human apolipoprotein E (Apo-E), a major component of very low-density lipoproteins (VLDLs). Apo-E is the principle apolipoprotein in the central nervous system, and is secreted by most organs into the plasma, playing a vital role in the binding, internalization and catabolism of triglyceride-rich lipoprotein constituents.

Apo-E acts as a ligand for both the specific apo-E receptor (chylomicron remnant) of hepatic tissues, and the apoB,E (LDL) receptor. Three isoforms of Apo-E have been identified, ApoE2, E3 and E4, and have been linked with various disorders. ApoE2 has been shown to bind LPL receptors with low affinity, resulting in increased plasma cholesterol and triglyceride levels, and thereby an increased risk in cardiovascular disorders. ApoE4 is a known high risk factor for Alzheimers disease, and in particular late onset Alzheimer disease 2 (AD2), whilst ApoE3 is the most common isoform, and considered the normal/natural Apo-E genotype.

Mouse anti Human Apolipoprotein E antibody, clone WUE-4 has been shown to inhibit Apo-E mediated binding of lipoproteins to the apoB,E cell receptor (Krul et al. 1998).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Sea Lion
Harbour seal
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Biotin - liquid

Product Form

Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Preservative Stabilisers

HRP Stabiliser (BUF052A) used in a 1:1 ratio

0.01% Thiomersal

Preservative Stabilisers

None present

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Carrier Free

Yes

Carrier Free

Yes

Immunogen

Purified ApoHDL fraction.

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/O-Ag14 mouse myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P02649 Related reagents
Entrez Gene: APOE Related reagents
GO Terms: GO:0000302 response to reactive oxygen species; GO:0001540 beta-amyloid binding; GO:0001937 negative regulation of endothelial cell proliferation; GO:0007010 cytoskeleton organization; GO:0005543 phospholipid binding; GO:0006641 triglyceride metabolic process; GO:0006898 receptor-mediated endocytosis; GO:0006916 anti-apoptosis; GO:0006917 induction of apoptosis; GO:0007186 G-protein coupled receptor protein signaling pathway; GO:0007263 nitric oxide mediated signal transduction; GO:0007271 synaptic transmission, cholinergic; GO:0008201 heparin binding; GO:0008219 cell death; GO:0010544 negative regulation of platelet activation; GO:0010873 positive regulation of cholesterol esterification; GO:0010875 positive regulation of cholesterol efflux; GO:0016209 antioxidant activity; GO:0019934 cGMP-mediated signaling; GO:0030425 dendrite; GO:0030516 regulation of axon extension; GO:0030828 positive regulation of cGMP biosynthetic process; GO:0032488 Cdc42 protein signal transduction; GO:0032805 positive regulation of low-density lipoprotein particle receptor catabolic process; GO:0033344 cholesterol efflux; GO:0033700 phospholipid efflux; GO:0034361 very-low-density lipoprotein particle; GO:0034362 low-density lipoprotein particle; GO:0034363 intermediate-density lipoprotein particle; GO:0034364 high-density lipoprotein particle; GO:0034372 very-low-density lipoprotein particle remodeling; GO:0034375 high-density lipoprotein particle remodeling; GO:0034380 high-density lipoprotein particle assembly; GO:0034382 chylomicron remnant clearance; GO:0034384 high-density lipoprotein particle clearance; GO:0034447 very-low-density lipoprotein particle clearance; GO:0046911 metal chelating activity; GO:0042627 chylomicron; GO:0042632 cholesterol homeostasis; GO:0042803 protein homodimerization activity; GO:0043025 neuronal cell body; GO:0043407 negative regulation of MAP kinase activity; GO:0043537 negative regulation of blood vessel endothelial cell migration; GO:0043691 reverse cholesterol transport; GO:0045541 negative regulation of cholesterol biosynthetic process; GO:0046907 intracellular transport; GO:0046982 protein heterodimerization activity; GO:0048156 tau protein binding; GO:0048168 regulation of neuronal synaptic plasticity; GO:0050728 negative regulation of inflammatory response; GO:0070326 very-low-density lipoprotein particle receptor binding; GO:0050750 low-density lipoprotein particle receptor binding; GO:0051000 positive regulation of nitric-oxide synthase activity; GO:0051044 positive regulation of membrane protein ectodomain proteolysis; GO:0060228 phosphatidylcholine-sterol O-acyltransferase activator activity
Regulatory: For research purposes only

Applications of Apolipoprotein E antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA
Western Blotting
ELISA	1/100	1/1000
Western Blotting
ELISA	1/100	1/1000
Functional Assays
Western Blotting
ELISA	1/100	1/1000
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: MCA5639B detects a major band of approximately 34-36kDa in human liver cell lysates.
Western Blotting: MCA5639P detects a major band of approximately 34-36kDa in human liver cell lysates.
Western Blotting: MCA5639EL detects a major band of approximately 34-36kDa in human liver cell lysates.
Western Blotting: MCA5639 detects a major band of approximately 34-36kDa in human liver cell lysates.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Application Based External Images

ELISA

Immunoprecipitation

Western Blotting

Product Specific References

References for Apolipoprotein E antibody

Krul, E.S. et al. (1988) Heterogeneity of apolipoprotein E epitope expression on human lipoproteins: importance for apolipoprotein E function.
J Lipid Res. 29 (10): 1309-25.
Fryer, J.D. et al. (2005) The low density lipoprotein receptor regulates the level of central nervous system human and murine apolipoprotein E but does not modify amyloid plaque pathology in PDAPP mice.
J Biol Chem. 280 (27): 25754-9.
Lee, C.Y. et al. (2012) Apolipoprotein E promotes β-amyloid trafficking and degradation by modulating microglial cholesterol levels.
J Biol Chem. 287: 2032-44.
Davis, R.W. et al. (1991) Lipoproteins in pinnipeds: analysis of a high molecular weight form of apolipoprotein E.
J Lipid Res. 32: 1013-23.
Wahrle, S.E. et al. (2007) Apolipoprotein E levels in cerebrospinal fluid and the effects of ABCA1 polymorphisms.
Mol Neurodegener. 2: 7.
Jiang, J. et al. (2012) Hepatitis C virus attachment mediated by apolipoprotein E binding to cell surface heparan sulfate.
J Virol. 86: 7256-67.
Hirsch-Reinshagen, V. et al. (2009) LCAT synthesized by primary astrocytes esterifies cholesterol on glia-derived lipoproteins.
J Lipid Res. 50: 885-93.
Wildsmith KR et al. (2012) In vivo human apolipoprotein E isoform fractional turnover rates in the CNS.
PLoS One. 7 (6): e38013.
Jiang, J. et al. (2013) Apolipoprotein e mediates attachment of clinical hepatitis C virus to hepatocytes by binding to cell surface heparan sulfate proteoglycan receptors.
PLoS One. 8: e67982.
Youmans, K.L. et al. (2011) Amyloid-β42 alters apolipoprotein E solubility in brains of mice with five familial AD mutations.
J Neurosci Methods. 196: 51-9.
Fan, J. et al. (2011) An ABCA1-independent pathway for recycling a poorly lipidated 8.1 nm apolipoprotein E particle from glia.
J Lipid Res. 52: 1605-16.
Fagan, A.M. et al. (2004) ApoAI deficiency results in marked reductions in plasma cholesterol but no alterations in amyloid-beta pathology in a mouse model of Alzheimer's disease-like cerebral amyloidosis.
Am J Pathol. 165: 1413-22.
Kim, J. et al. (2012) Anti-apoE immunotherapy inhibits amyloid accumulation in a transgenic mouse model of Aβ amyloidosis.
J Exp Med. 209: 2149-56.
Fu,Y. et al. (2016) Apolipoprotein E lipoprotein particles inhibit amyloid-β uptake through cell surface heparan sulphate proteoglycan.
Mol Neurodegener. 11 (1): 37.

View more products with Apolipoprotein E specificity