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Apolipoprotein E antibody | WUE-4

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Anti Human Apolipoprotein E Antibody, clone WUE-4 thumbnail image 1 Anti Human Apolipoprotein E Antibody, clone WUE-4 thumbnail image 2 Anti Human Apolipoprotein E Antibody, clone WUE-4 thumbnail image 3 Anti Human Apolipoprotein E Antibody, clone WUE-4 thumbnail image 4 Anti Human Apolipoprotein E Antibody, clone WUE-4 thumbnail image 5
Anti Human Apolipoprotein E Antibody, clone WUE-4 gallery image 1
Human apolipoprotein E detected with Mouse anti Human apolipoprotein E:HRP (MCA5639P).
Anti Human Apolipoprotein E Antibody, clone WUE-4 gallery image 2
Human apolipoprotein E detected with Mouse anti Human apolipoprotein E (MCA5639) followed by Rabbit F(ab')2 anti Mouse IgG:HRP (STAR13B)
Anti Human Apolipoprotein E Antibody, clone WUE-4 gallery image 3
Sandwich ELISA analysis of human apolipoprotein E using Mouse anti Human apolipoprotein E (MCA5639) as a capture reagnt and biotinylated Goat anti Human Apolipoprotein E (AHP2177) as a detection reagent with purified apolipoprotein E as antigen. Detection is with Streptavidin HRP. A serum sample 1/150 (green) and a plasma sample 1/150 (orange) are shown. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135).
Anti Human Apolipoprotein E Antibody, clone WUE-4 gallery image 4
Published investigator image:
Mouse anti Human Apolipoprotein E antibody, clone WUE-4 (MCA5639) used for the evaluation of Apo-E expression in culture media by western blotting.
Image caption:
Effects of the apoE-derived peptides and the HSPG-binding peptide 6a-P on heparin binding.
Heparin-immobilized beads (Pierce) were pre-equilibrated with PBS and then incubated with 500 μl of Huh-7.5 cell culture medium in the absence or presence of varying concentrations of the peptide hEP or 6a-P. The mutant peptides hEPm and 6a-Pm were used as controls. After 2 hrs incubation at room temperature, apoE-bound heparin-immobilized beads were spun down by centrifugation. The supernatant was collected and used for detection of the unbound apoE. The apoE-bound beads in pellet were washed three times with 1 ml of PBS. The heparin-bound and unbound apoE proteins were measured by Western blotting using an apoE-specific monoclonal antibody (WuE4).

From: Jiang J, Wu X, Tang H, Luo G (2013)
Apolipoprotein E Mediates Attachment of Clinical Hepatitis C Virus to Hepatocytes by Binding to Cell Surface Heparan Sulfate Proteoglycan Receptors.
PLoS ONE 8(7): e67982.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human Apolipoprotein E Antibody, clone WUE-4 gallery image 5
Sandwich ELISA analysis of human apolipoprotein E using Mouse anti Human apolipoprotein E (MCA5639) as a capture reagnt and biotinylated Goat anti Human Apolipoprotein E (AHP2177) as a detection reagent with purified apolipoprotein E as antigen. Detection is with Streptavidin HRP. A serum sample 1/150 (green) and a plasma sample 1/150 (orange) are shown. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135).

Mouse anti Human Apolipoprotein E:Biotin

Mouse anti Human Apolipoprotein E:Low Endotoxin

Mouse anti Human Apolipoprotein E

Product Type
Monoclonal Antibody
Clone
WUE-4
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA5639B
Datasheet
E WB
SDS
0.1 mg loader

MCA5639EL
Datasheet
E FN WB
SDS
0.5 mg loader

MCA5639GA
Datasheet
E WB
SDS
0.1 mg loader

MCA5639
Datasheet
E WB
SDS
0.2 mg loader

Mouse anti Human Apolipoprotein E antibody, clone WUE-4 recognizes an epitope within amino acids 140-160 of human apolipoprotein E (Apo-E), a major component of very low-density lipoproteins (VLDLs). Apo-E is the principle apolipoprotein in the central nervous system, and is secreted by most organs into the plasma, playing a vital role in the binding, internalization and catabolism of triglyceride-rich lipoprotein constituents.

Apo-E acts as a ligand for both the specific apo-E receptor (chylomicron remnant) of hepatic tissues, and the apoB,E (LDL) receptor. Three isoforms of Apo-E have been identified, ApoE2, E3 and E4, and have been linked with various disorders. ApoE2 has been shown to bind LPL receptors with low affinity, resulting in increased plasma cholesterol and triglyceride levels, and thereby an increased risk in cardiovascular disorders. ApoE4 is a high risk factor for Alzheimers disease (Sanan et al. 1994), and in particular late onset Alzheimer disease 2 (AD2), whilst ApoE3 is the most common isoform, and considered the normal/natural Apo-E genotype.

Mouse anti Human Apolipoprotein E antibody, clone WUE-4 has been shown to inhibit Apo-E mediated binding of lipoproteins to the apoB,E cell receptor (Krul et al. 1998).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sea Lion
Harbour seal
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Purified ApoHDL fraction.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/O-Ag14 mouse myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P02649
Entrez Gene
APOE
GO Terms
GO:0000302 response to reactive oxygen species
GO:0001540 beta-amyloid binding
GO:0001937 negative regulation of endothelial cell proliferation
GO:0007010 cytoskeleton organization
GO:0005543 phospholipid binding
GO:0006641 triglyceride metabolic process
GO:0006898 receptor-mediated endocytosis
GO:0006916 anti-apoptosis
GO:0006917 induction of apoptosis
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0007263 nitric oxide mediated signal transduction
GO:0007271 synaptic transmission, cholinergic
GO:0008201 heparin binding
GO:0008219 cell death
GO:0010544 negative regulation of platelet activation
GO:0010873 positive regulation of cholesterol esterification
GO:0010875 positive regulation of cholesterol efflux
GO:0016209 antioxidant activity
GO:0019934 cGMP-mediated signaling
GO:0030425 dendrite
GO:0030516 regulation of axon extension
GO:0030828 positive regulation of cGMP biosynthetic process
GO:0032488 Cdc42 protein signal transduction
GO:0032805 positive regulation of low-density lipoprotein particle receptor catabolic process
GO:0033344 cholesterol efflux
GO:0033700 phospholipid efflux
GO:0034361 very-low-density lipoprotein particle
GO:0034362 low-density lipoprotein particle
GO:0034363 intermediate-density lipoprotein particle
GO:0034364 high-density lipoprotein particle
GO:0034372 very-low-density lipoprotein particle remodeling
GO:0034375 high-density lipoprotein particle remodeling
GO:0034380 high-density lipoprotein particle assembly
GO:0034382 chylomicron remnant clearance
GO:0034384 high-density lipoprotein particle clearance
GO:0034447 very-low-density lipoprotein particle clearance
GO:0046911 metal chelating activity
GO:0042627 chylomicron
GO:0042632 cholesterol homeostasis
GO:0042803 protein homodimerization activity
GO:0043025 neuronal cell body
GO:0043407 negative regulation of MAP kinase activity
GO:0043537 negative regulation of blood vessel endothelial cell migration
GO:0043691 reverse cholesterol transport
GO:0045541 negative regulation of cholesterol biosynthetic process
GO:0046907 intracellular transport
GO:0046982 protein heterodimerization activity
GO:0048156 tau protein binding
GO:0048168 regulation of neuronal synaptic plasticity
GO:0050728 negative regulation of inflammatory response
GO:0070326 very-low-density lipoprotein particle receptor binding
GO:0050750 low-density lipoprotein particle receptor binding
GO:0051000 positive regulation of nitric-oxide synthase activity
GO:0051044 positive regulation of membrane protein ectodomain proteolysis
GO:0060228 phosphatidylcholine-sterol O-acyltransferase activator activity
Regulatory
For research purposes only

Applications of Apolipoprotein E antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
ELISA 1/100 1/1000
Functional Assays
Western Blotting
ELISA 1/100 1/1000
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA5639B detects a major band of approximately 34-36kDa in human liver cell lysates.
Western Blotting
MCA5639EL detects a major band of approximately 34-36kDa in human liver cell lysates.
Western Blotting
MCA5639 detects a major band of approximately 34-36kDa in human liver cell lysates.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

ELISA

Immunoprecipitation

Western Blotting

Product Specific References

References for Apolipoprotein E antibody

  1. Krul, E.S. et al. (1988) Heterogeneity of apolipoprotein E epitope expression on human lipoproteins: importance for apolipoprotein E function.
    J Lipid Res. 29 (10): 1309-25.
  2. Fryer, J.D. et al. (2005) The low density lipoprotein receptor regulates the level of central nervous system human and murine apolipoprotein E but does not modify amyloid plaque pathology in PDAPP mice.
    J Biol Chem. 280 (27): 25754-9.
  3. Lee, C.Y. et al. (2012) Apolipoprotein E promotes β-amyloid trafficking and degradation by modulating microglial cholesterol levels.
    J Biol Chem. 287: 2032-44.
  4. Davis, R.W. et al. (1991) Lipoproteins in pinnipeds: analysis of a high molecular weight form of apolipoprotein E.
    J Lipid Res. 32: 1013-23.
  5. Wahrle, S.E. et al. (2007) Apolipoprotein E levels in cerebrospinal fluid and the effects of ABCA1 polymorphisms.
    Mol Neurodegener. 2: 7.
  6. Jiang, J. et al. (2012) Hepatitis C virus attachment mediated by apolipoprotein E binding to cell surface heparan sulfate.
    J Virol. 86: 7256-67.
  7. Hirsch-Reinshagen, V. et al. (2009) LCAT synthesized by primary astrocytes esterifies cholesterol on glia-derived lipoproteins.
    J Lipid Res. 50: 885-93.
  8. Wildsmith KR et al. (2012) In vivo human apolipoprotein E isoform fractional turnover rates in the CNS.
    PLoS One. 7 (6): e38013.
  9. Jiang, J. et al. (2013) Apolipoprotein e mediates attachment of clinical hepatitis C virus to hepatocytes by binding to cell surface heparan sulfate proteoglycan receptors.
    PLoS One. 8: e67982.
  10. Youmans, K.L. et al. (2011) Amyloid-β42 alters apolipoprotein E solubility in brains of mice with five familial AD mutations.
    J Neurosci Methods. 196: 51-9.
  11. Fan, J. et al. (2011) An ABCA1-independent pathway for recycling a poorly lipidated 8.1 nm apolipoprotein E particle from glia.
    J Lipid Res. 52: 1605-16.
  12. Fagan, A.M. et al. (2004) ApoAI deficiency results in marked reductions in plasma cholesterol but no alterations in amyloid-beta pathology in a mouse model of Alzheimer's disease-like cerebral amyloidosis.
    Am J Pathol. 165: 1413-22.
  13. Kim, J. et al. (2012) Anti-apoE immunotherapy inhibits amyloid accumulation in a transgenic mouse model of Aβ amyloidosis.
    J Exp Med. 209: 2149-56.
  14. Fu,Y. et al. (2016) Apolipoprotein E lipoprotein particles inhibit amyloid-β uptake through cell surface heparan sulphate proteoglycan.
    Mol Neurodegener. 11 (1): 37.

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