Apolipoprotein A1 antibody | 1C5 (G2)
Mouse anti Human apolipoprotein A1, clone G2 (0650-0050) used to evaluate apolipoprotein A1 levels in patient samples by western blotting.
Image caption:
HDL-associated proteins. (A) Western blot analysis of AAT, PON-1 and APOA1 in isolated HDLs. (B) Quantification of HDL-associated PON1 and (C) AAT after normalization to APOA1 intensity in healthy donors (n = 12) and stroke patients with favorable (mRS 0–2, n = 22) or unfavorable (mRS 3–6, n = 24) outcomes. Values are means ± SEM. * p <0.05, ** p <0.01, *** p <0.001. Abbreviations: APOA1, apolipoprotein A1; PON-1, paraoxonase-1; AAT, alpha-1 anti-trypsin.
From:Varela LM, Meseguer E, Lapergue B, Couret D, Amarenco P, Meilhac O.
Changes in High-Density Lipoproteins Related to Outcomes in Patients with Acute Stroke.
J Clin Med. 2020 Jul 17;9(7):2269.
doi: 10.3390/jcm9072269.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human apolipoprotein A1, clone 1C5 (0650-0050) used to evaluate Apo-A1 levels in rabbit plasma samples by western blotting.
Image caption:
Analysis of plasma lipids, apolipoproteins, and lipoproteins in APOC3-KO rabbits. b Analysis of plasma apolipoproteins by western blotting. Rabbit plasma samples (8 μL) were electrophoresed on SDS-PAGE using 10% gels, transferred to a cellulose membrane, and probed with appropriate antibodies. ^, # and *: The content of plasma APOE, APOA1 and PCSK9 in AC1-AC3 compared with WT.
From: Zha Y, Lu Y, Zhang T, Yan K, Zhuang W, Liang J, Cheng Y, Wang Y.
CRISPR/Cas9-mediated knockout of APOC3 stabilizes plasma lipids and inhibits atherosclerosis in rabbits.
Lipids Health Dis. 2021 Dec 18;20(1):180.
doi: 10.1186/s12944-021-01605-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human apolipoprotein A1, clone 1C5 (0650-0050) used to assess Apo-A1 levels in rabbit plasma samples by western blotting.
Image caption:
CBS-KO rabbits are hyperlipidemic. Immunoblot showing levels of plasma apolipoproteins in the indicated groups. WT-NC: wild type rabbits fed normal chow. WT-SB: wild type rabbits fed normal chow supplemented with the vitamin B and betaine complex. C2♀, C8♀: CBS-KO rabbits on a normal chow diet. C9♀, C14♂, C15♀, C16♂: CBS-KO rabbits fed normal chow supplemented with the vitamin B and betaine complex
From: Zhang T, Lu R, Chen Y, Yuan Y, Song S, Yan K, Zha Y, Zhuang W, Cheng Y, Liang J.
Hyperhomocysteinemia and dyslipidemia in point mutation G307S of cystathionine β-synthase-deficient rabbit generated using CRISPR/Cas9.
Lipids Health Dis. 2020 Oct 14;19(1):224.
doi: 10.1186/s12944-020-01394-5.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Apolipoprotein A1
- Product Type
- Monoclonal Antibody
- Clone
- 1C5 (G2)
- Isotype
- IgG1
- Specificity
- Apolipoprotein A1
Antibody Quality and Performance Guarantee
| Mouse anti Human apolipoprotein A1, clone G2 recognizes Apolipoprotein A-1 (also known as Apo-A1) , a lipid-binding protein which enables the transport of dietary lipids for storage, metabolism and secretion. Apo-A1 plays an important part in the removal of cholesterol from cells. Mouse anti Human apolipoprotein A1, clone G2 reacts with both free human Apo-A1 and High Density Lipoprotein (HDL) bearing Apo-A1, but does not cross-react with ApoE, ApoB or albumin. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- 0.01M Sodium Phosphate
0.01M Sodium Borate
0.15M Sodium Chloride - Preservative Stabilisers
- 1% Dextran
1% Mannitol - Immunogen
- Native Apolipoprotein-A1 from human plasma
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/5000 | |
| Immunohistology - Frozen | |
1/40 | |
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- ELISA
- This antibody is suitable for coating microtitre plates in a sandwich ELISA using catalogue number 0650-0190 for detection.
References for Apolipoprotein A1 antibody
-
Derbali, H. et al. (2010) Increased biglycan in aortic valve stenosis leads to the overexpression of phospholipid transfer protein via Toll-like receptor 2.
Am J Pathol. 176: 2638-45. -
Mogilenko, D.A. et al. (2012) Endogenous apolipoprotein A-I stabilizes ATP-binding cassette transporter A1 and modulates Toll-like receptor 4 signaling in human macrophages.
FASEB J. 26: 2019-30. -
Berge, K.E. et al. (2014) Type 1 hyperlipoproteinemia due to a novel deletion of exons 3 and 4 in the GPIHBP1 gene.
Atherosclerosis. 234 (1): 30-3. -
Pingitore, P. et al. (2016) Identification and characterization of two novel mutations in the LPL gene causing type I hyperlipoproteinemia.
J Clin Lipidol. 10 (4): 816-23. -
Shavva, V.S. et al. (2016) PPARγ Represses Apolipoprotein A-I Gene but Impedes TNFα-Mediated ApoA-I Downregulation in HepG2 Cells.
J Cell Biochem. 117 (9): 2010-22. -
Shavva, V.S. et al. (2018) Tumor necrosis factor α stimulates endogenous apolipoprotein A-I expression and secretion by human monocytes and macrophages: role of MAP-kinases, NF-κB, and nuclear receptors PPARα and LXRs.
Mol Cell Biochem. 448 (1-2): 211-223. -
Botta, M. et al. (2019) Deciphering the role of V200A and N291S mutations leading to LPL deficiency.
Atherosclerosis. 282: 45-51. -
Varela, L.M. et al. (2020) Changes in High-Density Lipoproteins Related to Outcomes in Patients with Acute Stroke.
J Clin Med. 9 (7): 2269.
View The Latest Product References
-
Zha, Y. et al. (2021) CRISPR/Cas9-mediated knockout of APOC3 stabilizes plasma lipids and inhibits atherosclerosis in rabbits.
Lipids Health Dis. 20 (1): 180. -
Zhang, T. et al. (2020) Hyperhomocysteinemia and dyslipidemia in point mutation G307S of cystathionine β-synthase-deficient rabbit generated using CRISPR/Cas9.
Lipids Health Dis. 19 (1): 224.
- RRID
- AB_620040
- UniProt
- P02647
- Entrez Gene
- APOA1
- GO Terms
- GO:0005886 plasma membrane
- GO:0001540 beta-amyloid binding
- GO:0002576 platelet degranulation
- GO:0002740 negative regulation of cytokine secretion involved in immune response
- GO:0005543 phospholipid binding
- GO:0005788 endoplasmic reticulum lumen
- GO:0030141 stored secretory granule
- GO:0030139 endocytic vesicle
- GO:0006656 phosphatidylcholine biosynthetic process
- View More GO Terms
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0042802 identical protein binding
- GO:0010873 positive regulation of cholesterol esterification
- GO:0010903 negative regulation of very-low-density lipoprotein particle remodeling
- GO:0015485 cholesterol binding
- GO:0050821 protein stabilization
- GO:0017127 cholesterol transporter activity
- GO:0019899 enzyme binding
- GO:0030168 platelet activation
- GO:0032488 Cdc42 protein signal transduction
- GO:0033344 cholesterol efflux
- GO:0033700 phospholipid efflux
- GO:0034191 apolipoprotein A-I receptor binding
- GO:0034361 very-low-density lipoprotein particle
- GO:0034366 spherical high-density lipoprotein particle
- GO:0034375 high-density lipoprotein particle remodeling
- GO:0034380 high-density lipoprotein particle assembly
- GO:0034384 high-density lipoprotein particle clearance
- GO:0042632 cholesterol homeostasis
- GO:0043691 reverse cholesterol transport
- GO:0050713 negative regulation of interleukin-1 beta secretion
- GO:0051345 positive regulation of hydrolase activity
- GO:0060228 phosphatidylcholine-sterol O-acyltransferase activator activity
- GO:0070508 cholesterol import
- GO:0070653 high-density lipoprotein particle receptor binding
View more products with APOLIPOPROTEIN specificity
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